Mouse CRISP-1 Antibody

Catalog #: AF4675 Datasheet / COA / SDS

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AF4675
AF4675-SP

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Detection of Mouse CRISP‑1 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CRISP-1 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CRISP-1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse CRISP-3 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CRISP-1
Gln20-His244
Accession # Q03401

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.2 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse CRISP‑1 by Western Blot. Western blot shows lysates of mouse testis tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse CRISP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4675) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CRISP-1 at approximately 25-29 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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CRISP‑1 in Mouse Epididymus. CRISP-1 was detected in immersion fixed frozen sections of mouse epididymus using Goat Anti-Mouse CRISP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4675) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CRISP-1

Cysteine‑rich secretory protein 1 (CRISP‑1), also known as Acidic Epididymal Glycoprotein 1 (AEG1) and Sperm‑Coating Glycoprotein 1 (SCP1), is a 29-32 kDa (26.4 kDa predicted) protein that is involved in male reproductive biology (1, 2). CRISPs belong to the CAP superfamily of molecules that also includes several snake, insect, and lizard venom proteins (3). Structurally, CRISPs consist of an N‑terminal SCP/CAP domain, a hinge region, and a Cys‑rich domain with 16 invariant cysteine residues (4). CRISPs are expressed in discrete but overlapping regions of the male reproductive tract and play a role in spermatozoa adhesion with Sertoli cells and oocytes, decapacitation, and the acrosomal reaction (1). Expression patterns, genomic structure, and sequence conservation indicate that the likely ortholog of human CRISP‑1 in mouse and rat is CRISP‑4 (5, 6). Mature mouse CRISP‑1 shares 68% amino acid (aa) sequence identity with rat CRISP‑1. It shares 55%, 73%, and 43% aa sequence identity with mouse CRISP‑2, ‑3, and ‑4, respectively. Rodent CRISP‑1 is secreted by the epididymal epithelium and associates with the surface of spermatozoa in the epididymis where it inhibits capacitation (7‑9). It is released from the sperm cell surface once capacitation begins in the female reproductive tract or in vitro (10). CRISP‑1 is involved in the interaction of spermatozoa with the oocyte zona pellucida as well as in the fusion of sperm and egg (11, 12). CRISP‑1 is additionally expressed in the lower medulla of hair shafts and under androgen control in the submandibular salivary glands of male mice (13‑15).

References

Koppers, A.J. et al. (2011) Asian J. Androl. 13:111.
Gibbs, G.M. et al. (2008) Endocr. Rev. 29:865.
Yamazaki, Y. and T. Morita (2004) Toxicon 44:227.
Guo, M. et al. (2005) J. Biol. Chem. 280:12405.
Jalkanen, J. et al. (2005) Biol. Reprod. 72:1268.
Nolan, M.A. et al. (2006) Biol. Reprod. 74:984.
Charest, N.J. et al. (1989) Endocrinology 125:942.
Maldera, J.A. et al. (2011) Biol. Reprod. 85:503.
Nixon, B. et al. (2006) Biol. Reprod. 74:275.
Roberts, K.P. et al. (2003) Biol. Reprod. 69:572.
Busso, D. et al. (2007) Biol. Reprod. 77:848.
Da Ros, V.G. et al. (2008) Dev. Biol. 320:12.
Haendler, B. et al. (1993) Endocrinology 133:192.
Mizuki, N. and M. Kasahara (1992) Mol. Cell. Endocrinol. 89:25.
Peterson, R.L. et al. (2005) J. Investig. Dermatol. Symp. Proc. 10:238.

Long Name

Cysteine-rich Secretory Protein 1

Entrez Gene IDs

167 (Human); 11571 (Mouse); 64827 (Rat)

Alternate Names

Acidic epididymal glycoprotein homolog; acidic epididymal glycoprotein-like 1; AEGL1; AEG-related protein; ARPAEG-like protein; CRISP1; CRISP-1; CRISP-1AEGL1HSCRISP1D; cysteine-rich secretory protein 1; cysteine-rich secretory protein-1 delta; HSCRISP1G; HUMARP