Mouse CTLA-4 Antibody

Catalog # Availability Size / Price Qty
MAB434-SP
MAB434-100
MAB434-500
CTLA‑4 Inhibition of B7‑1/CD80-induced IL‑2 Secretion and Neutralization by Mouse CTLA‑4 Antibody.
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Mouse CTLA-4 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CTLA-4 in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with recombinant mouse (rm) CD28, recombinant human CTLA-4, rmICOS, or rmPD-1.
Source
Monoclonal Rat IgG2A Clone # 63828
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CTLA-4
Ala36-Phe161
Accession # XP_001479180
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse CTLA‑4 Fc Chimera (Catalog # 434-CT) under non-reducing conditions only
Flow Cytometry
2.5 µg/106 cells
Mouse splenocytes treated with ConA
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Neutralization
Measured by its ability to neutralize CTLA‑4 inhibition of B7‑1/CD80-induced IL‑2 secretion in the Jurkat human acute T cell leukemia cell line. Linsley, P. et al. (1990) Proc. Natl. Acad. Sci. USA 87:5031. The Neutralization Dose (ND50) is typically 2.5-10 µg/mL in the presence of 1 µg/mL Recombinant Mouse CTLA‑4 Fc Chimera, 3 µg/mL Recombinant Human B7‑1/CD80 Fc Chimera, and 10 µg/mL PHA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization CTLA‑4 Inhibition of B7‑1/CD80-induced IL‑2 Secretion and Neutralization by Mouse CTLA‑4 Antibody. View Larger

CTLA‑4 Inhibition of B7‑1/CD80-induced IL‑2 Secretion and Neutralization by Mouse CTLA‑4 Antibody. Recombinant Mouse CTLA‑4 Fc Chimera (Catalog # 434-CT) inhibits Recombinant Human B7‑1/CD80 Fc Chimera (Catalog # 140-B1) induced IL‑2 secretion in the Jurkat human acute T cell leukemia cell line in a dose-dependent manner (orange line), as measured by the Human IL‑2 Quantikine ELISA Kit (Catalog # D2050). Inhibition of Recombinant Human B7‑1/CD80 Fc Chimera (3 µg/mL) activity elicited by Recombinant Mouse CTLA‑4 Fc Chimera (1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse CTLA‑4 Monoclonal Antibody (Catalog # MAB434). The ND50 is typically 2.5-10 µg/mL in the presence of PHA (10 µg/mL).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CTLA-4

CTLA-4 and CD28, together with their ligands B7-1 and B7-2, constitute one of the dominant costimulatory pathways that regulate T and B cell responses. CTLA-4 and CD28 are structurally homologous molecules that are members of the immunoglobulin (Ig) gene superfamily. Both CTLA-4 and CD28 are composed of a single Ig V‑like extracellular domain, a transmembrane domain and an intracellular domain. CTLA-4 and CD28 are both expressed on the cell surface as disulfide-linked homodimers or as monomers. The genes encoding these two molecules are closely linked on human chromosome 2. CTLA-4 was originally identified as a gene that was specifically expressed by cytotoxic T lymphocytes. However, CTLA-4 transcripts have since been found in both Th1 and Th2, and CD4+ and CD8+ T cell clones. Whereas, CD28 expression is constitutive on the surfaces of 95% of CD4+ T cells and 50% of CD8+ T cells and is down regulated upon T cell activation, CTLA-4 expression is upregulated rapidly following T cell activation and peaks approximately 24 hours following activation. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with 20-100-fold higher affinity than CD28. The physiological role of CTLA-4 in T cell costimulation is currently being studied. Recombinant human or mouse CTLA-4/Fc chimera preparations produced at R&D Systems have been shown to bind both B7-1 and B7-2 with high affinity and to inhibit CD28 signalling competitively.

References
  1. Lenschow, D.J. et al. (1996) Annu. Rev. Immunol. 14:233.
  2. Hathcock, K.S. and R.J. Hodes (1996) Advances in Immunol. 62:131.
  3. Ward, S.G. (1996) Biochem. J. 318:361.
Long Name
Cytotoxic T-lymphocyte-associated Molecule 4
Entrez Gene IDs
1493 (Human); 12477 (Mouse); 403696 (Canine); 102115124 (Cynomolgus Monkey)
Alternate Names
CD; CD152 antigen; CD152; CD152IDDM12; CD28; celiac disease 3; CELIAC3; CTLA4; CTLA-4; cytotoxic T-lymphocyte antigen 4; cytotoxic T-lymphocyte protein 4; Cytotoxic T-lymphocyte-associated antigen 4; cytotoxic T-lymphocyte-associated protein 4; cytotoxic T-lymphocyte-associated serine esterase-4; GRD4; GSE; ICOS; ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4

Product Datasheets

Citation for Mouse CTLA-4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Dopamine, through the extracellular signal-regulated kinase pathway, downregulates CD4+CD25+ regulatory T-cell activity: implications for neurodegeneration.
    Authors: Kipnis J, Cardon M, Avidan H, Lewitus GM, Mordechay S, Rolls A, Shani Y, Schwartz M
    J. Neurosci., 2004;24(27):6133-43.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay

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