Mouse DNER Antibody

Catalog #: AF2254
6 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2254
AF2254-SP

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Citations (6)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse DNER Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse DNER in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 40% cross-reactivity with recombinant human DNER is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse DNER
Ala26-His637
Accession # Q8JZM4

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse DNER (Catalog # 2254-DN)

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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DNER in Mouse Brain. DNER was detected in perfusion fixed frozen sections of mouse brain (cerebellum) using Goat Anti-Mouse DNER Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2254) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to Purkinje neurons and molecular layer. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DNER

DNER, also known as BET, is a type I transmembrane glycoprotein that is specifically expressed on nonaxonal areas of post-mitotic neurons. The protein has an extracellular domain containing ten distinct EGF-like repeats similar to those found on Delta and Notch. Human and mouse DNER share 90% amino acid sequence identity.

Long Name

Delta/Notch-like EGF Repeat-containing Transmembrane

Entrez Gene IDs

92737 (Human); 227325 (Mouse)

Alternate Names

bet; delta and Notch-like epidermal growth factor-related receptor; delta/notch-like EGF repeat containing; delta-notch-like EGF repeat-containing transmembrane; DNER; H_NH0150O02.1; UNQ26; WUGSC:H_NH0150O02.1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse DNER Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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AAV-mediated delivery of an anti-BACE1 VHH alleviates pathology in an Alzheimer's disease model
Authors: M Marino, L Zhou, MY Rincon, Z Callaerts-, J Verhaert, J Wahis, E Creemers, L Yshii, K Wierda, T Saito, C Marneffe, I Voytyuk, Y Wouters, M Dewilde, SI Duqué, C Vincke, Y Levites, TE Golde, TC Saido, S Muylderman, A Liston, B De Stroope, MG Holt
Embo Molecular Medicine, 2022;14(4):e09824.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot
A conserved YAP/Notch/REST network controls the neuroendocrine cell fate in the lungs
Authors: YT Shue, AP Drainas, NY Li, SM Pearsall, D Morgan, N Sinnott-Ar, SQ Hipkins, GL Coles, JS Lim, AE Oro, KL Simpson, C Dive, J Sage
Nature Communications, 2022;13(1):2690.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
The Notch ligand DNER regulates macrophage IFN? release in chronic obstructive pulmonary disease
Authors: C Ballester-, TM Conlon, Z Ertüz, FR Greiffo, M Irmler, SE Verleden, J Beckers, IE Fernandez, O Eickelberg, AÖ Yildirim
EBioMedicine, 2019;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P
DNER modulates the length, polarity and synaptogenesis of spiral ganglion neurons via the Notch signaling pathway
Authors: J Du, X Wang, X Zhang, X Zhang, H Jiang
Mol Med Rep, 2018;17(2):2357-2365.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
Delta/Notch-Like EGF-Related Receptor (DNER) Is Not a Notch Ligand
PLoS ONE, 2016;11(9):e0161157.
Species: Human
Sample Types: Whole Cells
Applications: ICC
A search for factors specifying tonotopy implicates DNER in hair-cell development in the chick's cochlea.
Authors: Kowalik L, Hudspeth AJ
Dev. Biol., 2011;354(2):221-31.
Species: Chicken
Sample Types: Whole Tissue
Applications: IHC-Fr