Mouse DPPA5/ESG1 Antibody

Catalog # Availability Size / Price Qty
AF3984
AF3984-SP
Product Details
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Mouse DPPA5/ESG1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse DPPA5/ESG1 in direct ELISAs and Western blots. In Western blots, approximately 25% cross-reactivity with recombinant human DPPA5 is observed and approximately 10% cross-reactivity with recombinant mouse DPPA4 is observed. 
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse DPPA5/ESG1
Met1-Glu118
Accession # Q9CQS7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse DPPA5/ESG1
Immunocytochemistry
5-15 µg/mL
Immersion fixed D3 mouse embryonic stem cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DPPA5/ESG1

DPPA5, also known as Esg-1 and ECAT2, is a 14 kDa RNA-binding protein expressed in early embryos, primordial germ cells, and embryonic stem cells and is used as a marker of the pluripotent state. Mouse DPPA5 is 118 amino acids in length and shows 63% amino acid identity with human and 81% amino acid identity with rat DPPA5 proteins.

Long Name
Developmental Pluripotency Associated 5
Entrez Gene IDs
340168 (Human); 434423 (Mouse); 301101 (Rat)
Alternate Names
developmental pluripotency associated 5; developmental pluripotency-associated 5 protein; DPPA5; embryonal stem cell specific gene 1; Embryonal stem cell-specific gene 1 protein; ESG1; ESG1ESG-1; hDPPA5

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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