Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all DPPA5/ESG1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse DPPA5/ESG1
Met1-Glu118
Accession # Q9CQS7

Specificity

Detects mouse DPPA5/ESG1 in direct ELISAs and Western blots. In Western blots, approximately 25% cross-reactivity with recombinant human DPPA5 is observed and approximately 10% cross-reactivity with recombinant mouse DPPA4 is observed. 

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse DPPA5/ESG1 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed D3 mouse embryonic stem cell line

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse DPPA5/ESG1

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DPPA5/ESG1

DPPA5, also known as Esg-1 and ECAT2, is a 14 kDa RNA-binding protein expressed in early embryos, primordial germ cells, and embryonic stem cells and is used as a marker of the pluripotent state. Mouse DPPA5 is 118 amino acids in length and shows 63% amino acid identity with human and 81% amino acid identity with rat DPPA5 proteins.

Long Name

Developmental Pluripotency Associated 5

Alternate Names

ESG1

Entrez Gene IDs

340168 (Human); 434423 (Mouse); 301101 (Rat)

Gene Symbol

DPPA5

UniProt

Q9CQS7

Additional DPPA5/ESG1 Products

Product Documents for Mouse DPPA5/ESG1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse DPPA5/ESG1 Antibody

For research use only

Related Research Areas

Primordial and Mature Germ Cells

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Protocols

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FAQs

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