Mouse DPPIV/CD26 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF954
Product Details
FAQs
Supplemental Products
Reviews

Mouse DPPIV/CD26 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse DPPIV/CD26 in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant human DPPIV/CD26 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse DPPIV/CD26
Ser29-His760
Accession # P28843
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse DPPIV/CD26 (Catalog # 954-SE)
Immunohistochemistry
5-15 µg/mL
Perfusion fixed frozen sections of mouse thymus

Mouse DPPIV/CD26 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Mouse DPPIV/CD26 Antibody (Catalog # MAB954)

Standard: Recombinant Mouse DPPIV/CD26 Protein, CF (Catalog # 954-SE)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DPPIV/CD26

DPPIV/CD26 (EC 3.4.14.5) is a serine exopeptidase that releases Xaa-Pro dipeptides from the N-terminus of oligo- and polypeptides (1, 2). It is a type II membrane protein consisting of a short cytoplasmic tail, a transmembrane domain, and a long extracellular domain (3‑5). The extracellular domain contains glycosylation sites, a cysteine-rich region and the catalytic active site (Ser, Asp and His charge relay system). The amino acid sequence of the mouse DPPIV/CD26 extracellular domain is 84% and 91% identical to the human and rat counterparts, respectively. In the native state, DPPIV/CD26 is present as a noncovalently linked homodimer on the cell surface of a variety of cell types. The soluble form is also detectable in human serum and other body fluids, the levels of which may have clinical significance in patients with cancer, liver and kidney diseases, and depression.

DPPIV/CD26 plays an important role in many biological and pathological processes. It functions as T cell-activating molecule (THAM). It serves as a co‑factor for entry of HIV in CD4+ cells (6). It binds adenosine deaminase, the deficiency of which causes severe combined immunodeficiency disease in humans (7). It cleaves chemokines such as stromal-cell-derived factor 1 alpha and macrophage-derived chemokine (8, 9). It degrades peptide hormones such as glucagon (10). It truncates procalcitonin, a marker for systemic bacterial infections with elevated levels detected in patients with thermal injury, sepsis and severe infection, and in children with bacterial meningitis (11).

References
  1. Misumi, Y. and Y. Ikehara (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. (eds), p. 1905, Elsevier, London.
  2. Ikehara, Y. et al. (1994) Methods Enzymol. 244:215.
  3. Marguet, D. et al. (1992) J. Biol. Chem. 267:2200.
  4. Bernard, A.M. et al. (1994) Biochemistry 33:15204.
  5. Vivier, I. et al. (1991) J. Immunol. 147:447.
  6. Callebaut, C. et al. (1993) Science 262:2045.
  7. Kameoka, J. et al. (1993) Science 261:466.
  8. Ohtsuki, T. et al. (1998) FEBS Lett. 431:236.
  9. Proost, P. et al. (1999) J. Biol. Chem. 274:3988.
  10. Hinke, S.A. et al. (2000) J. Biol. Chem. 275:3827.
  11. Wrenger, S. et al. (2000) FEBS Lett. 466:155.
Long Name
Dipeptidyl-peptidase IV
Entrez Gene IDs
1803 (Human); 13482 (Mouse); 102133935 (Cynomolgus Monkey)
Alternate Names
ADABP; ADCP-2; ADCP2DPP IV; Adenosine deaminase complexing protein 2TP103; CD26 antigen; CD26; CD26T-cell activation antigen CD26; dipeptidyl peptidase 4; Dipeptidyl peptidase IV; dipeptidylpeptidase 4; dipeptidyl-peptidase 4; dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2); DPP4; DPPIV; EC 3.4.14.5

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Staining Reagents

Supplemental Cell Selection Products

Supplemental ELISA Products

Reviews for Mouse DPPIV/CD26 Biotinylated Antibody

There are currently no reviews for this product. Be the first to review Mouse DPPIV/CD26 Biotinylated Antibody and earn rewards!

Have you used Mouse DPPIV/CD26 Biotinylated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review