Mouse DPPIV/CD26 Antibody

(6 citations)
(2 Reviews)
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse DPPIV/CD26 in direct ELISAs and Western blots. In direct ELISAs, less than  20% cross‑reactivity with recombinant human DPPIV/CD26 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse DPPIV/CD26
    Ser29-His760
    Accession # P28843
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    .25 µg/mL
    See below
  • Simple Western
    5 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse and Rat DPPIV/CD26 by Western Blot. Western blot shows lysates of mouse thymus tissue and rat lung tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF954) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for DPPIV/CD26 at approximately 100-110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
DPPIV/CD26 in Mouse Thymus. DPPIV/CD26 was detected in immersion fixed frozen sections of mouse thymus using Goat Anti-Mouse DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF954) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Detection of Mouse and Rat DPPIV/CD26 by Simple WesternTM. Simple Western lane view shows lysates of rat lung tissue and mouse thymus tissue, loaded at 0.2 mg/mL. A specific band was detected for DPPIV/CD26 at approximately 144-169 kDa (as indicated) using 5 µg/mL of Goat Anti-Mouse DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF954) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody
(Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.        
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DPPIV/CD26

DPPIV/CD26 (EC 3.4.14.5) is a serine exopeptidase that releases Xaa-Pro dipeptides from the N-terminus of oligo- and polypeptides (1, 2). It is a type II membrane protein consisting of a short cytoplasmic tail, a transmembrane domain, and a long extracellular domain (3‑5). The extracellular domain contains glycosylation sites, a cysteine-rich region and the catalytic active site (Ser, Asp and His charge relay system). The amino acid sequence of the mouse DPPIV/CD26 extracellular domain is 84% and 91% identical to the human and rat counterparts, respectively. In the native state, DPPIV/CD26 is present as a noncovalently linked homodimer on the cell surface of a variety of cell types. The soluble form is also detectable in human serum and other body fluids, the levels of which may have clinical significance in patients with cancer, liver and kidney diseases, and depression.

DPPIV/CD26 plays an important role in many biological and pathological processes. It functions as T cell-activating molecule (THAM). It serves as a co‑factor for entry of HIV in CD4+ cells (6). It binds adenosine deaminase, the deficiency of which causes severe combined immunodeficiency disease in humans (7). It cleaves chemokines such as stromal-cell-derived factor 1 alpha and macrophage-derived chemokine (8, 9). It degrades peptide hormones such as glucagon (10). It truncates procalcitonin, a marker for systemic bacterial infections with elevated levels detected in patients with thermal injury, sepsis and severe infection, and in children with bacterial meningitis (11).

  • References:
    1. Misumi, Y. and Y. Ikehara (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. (eds), p. 1905, Elsevier, London.
    2. Ikehara, Y. et al. (1994) Methods Enzymol. 244:215.
    3. Marguet, D. et al. (1992) J. Biol. Chem. 267:2200.
    4. Bernard, A.M. et al. (1994) Biochemistry 33:15204.
    5. Vivier, I. et al. (1991) J. Immunol. 147:447.
    6. Callebaut, C. et al. (1993) Science 262:2045.
    7. Kameoka, J. et al. (1993) Science 261:466.
    8. Ohtsuki, T. et al. (1998) FEBS Lett. 431:236.
    9. Proost, P. et al. (1999) J. Biol. Chem. 274:3988.
    10. Hinke, S.A. et al. (2000) J. Biol. Chem. 275:3827.
    11. Wrenger, S. et al. (2000) FEBS Lett. 466:155.
  • Long Name:
    Dipeptidyl-peptidase IV
  • Entrez Gene IDs:
    1803 (Human); 13482 (Mouse); 102133935 (Cynomolgus Monkey)
  • Alternate Names:
    ADABP; ADCP-2; ADCP2DPP IV; Adenosine deaminase complexing protein 2TP103; CD26 antigen; CD26; CD26T-cell activation antigen CD26; dipeptidyl peptidase 4; Dipeptidyl peptidase IV; dipeptidylpeptidase 4; dipeptidyl-peptidase 4; dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2); DPP4; DPPIV; EC 3.4.14.5
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Species
Applications
Sample Type
  1. Effect of Antifibrotic MicroRNAs Crosstalk on the Action of N-acetyl-seryl-aspartyl-lysyl-proline in Diabetes-related Kidney Fibrosis
    Sci Rep, 2016;6(0):29884.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Not Specified
  2. Contribution of Mature Hepatocytes to Biliary Regeneration in Rats with Acute and Chronic Biliary Injury.
    Authors: Chen Y, Chen H, Chien C, Wu S, Ho Y, Yu C, Chang M
    PLoS ONE, 2015;10(8):e0134327.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC Not Specified
  3. Linagliptin-mediated DPP-4 inhibition ameliorates kidney fibrosis in streptozotocin-induced diabetic mice by inhibiting endothelial-to-mesenchymal transition in a therapeutic regimen.
    Authors: Kanasaki K, Shi S, Kanasaki M, He J, Nagai T, Nakamura Y, Ishigaki Y, Kitada M, Srivastava S, Koya D
    Diabetes, 2014;63(6):2120-31.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  4. Distinct fibroblast lineages determine dermal architecture in skin development and repair.
    Authors: Driskell R, Lichtenberger B, Hoste E, Kretzschmar K, Simons B, Charalambous M, Ferron S, Herault Y, Pavlovic G, Ferguson-Smith A, Watt F
    Nature, 2013;504(7479):277-81.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  5. KDR identifies a conserved human and murine hepatic progenitor and instructs early liver development.
    Authors: Goldman O, Han S, Sourisseau M, Dziedzic N, Hamou W, Corneo B, D'Souza S, Sato T, Kotton D, Bissig K, Kalir T, Jacobs A, Evans T, Evans M, Gouon-Evans V
    Cell Stem Cell, 2013;12(6):748-60.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  6. Inhibition of dipeptidyl peptidase 4 regulates microvascular endothelial growth induced by inflammatory cytokines.
    Authors: Takasawa W, Ohnuma K, Hatano R, Endo Y, Dang NH, Morimoto C
    Biochem. Biophys. Res. Commun., 2010;401(1):7-12.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
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Average Rating: 5 (Based on 2 reviews)

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We have 2 reviews tested in 2 applications: Immunocytochemistry/Immunofluorescence, Immunoprecipitation.

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Images Ratings Applications Species Reviewed By Date Details
Immunocytochemistry/Immunofluorescence Mouse DPPIV/CD26 Antibody AF954
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Excellent
 ICC/IF Human Anonymous 10/18/2016
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Image Details
Immunocytochemistry/Immunofluorescence Mouse DPPIV/CD26 Antibody AF954
Immunocytochemistry/Immunofluorescence: Mouse DPPIV/CD26 Antibody [AF954]

Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample Testedmesenchymal cells
SpeciesHuman
  Excellent
 IP Mouse Anonymous 08/11/2016
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Summary

ApplicationImmunoprecipitation
Sample TestedHeparin Plasma
SpeciesMouse

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