Mouse EphA3 Antibody

Catalog # Availability Size / Price Qty
AF640
AF640-SP
EphA3 in Mouse Embryo.
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Product Details
Citations (4)
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Mouse EphA3 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse EphA3 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse (rm) EphA4, rmEphA8, recombinant rat EphA5, and rmEphA6 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse EphA3
Glu21-His541 (Thr323Ala, Glu476Gln)
Accession # Q8BRB1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse EphA3 Fc Chimera (Catalog # 640-A3)
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry EphA3 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

EphA3 in Mouse Embryo. EphA3 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Mouse EphA3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF640) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to developing brain. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EphA3

EphA3, also known as Cek4, Mek4, Hek, Tyro4, and Hek4 (1), is a member of the Eph receptor family which binds members of the ephrin ligand family. There are two classes of receptors, designated A and B. Both the A and B class receptors have an extracellular region consisting of a globular domain, a cysteine-rich domain, and two fibronectin type III domains, followed by the transmembrane region and cytoplasmic region. The cytoplasmic region contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxy tail which contains one conserved tyrosine residue. Activation of kinase activity occurs after ligand recognition and binding. EphA3 has been shown to bind ephrin-A5, ephrin-A2, ephrin-A3, ephrin-A1, ephrin-A4, and ephrin-B1 (2, 3). The extracellular domains of mouse and human EphA3 share greater than 96% amino acid identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/ephrin families also appear to play a role in angiogenesis (3).

References
  1. Eph Nomenclature Committee [letter] (1997) Cell 90:403.
  2. Flanagan, J.G. and P. Vanderhaegen (1998) Annu. Rev. Neurosci. 21:309.
  3. Pasquale, E.B. (1997) Curr. Opin. Cell. Biol. 9:608.
Long Name
Eph Receptor A3
Entrez Gene IDs
2042 (Human); 13837 (Mouse)
Alternate Names
Cek4; EC 2.7.10; EC 2.7.10.1; EK4; EPH receptor A3; EphA3; EPH-like kinase 4; ephrin type-A receptor 3; ETK; Hek4; HEKETK1eph-like tyrosine kinase 1; human embryo kinase 1; TYRO4 protein tyrosine kinase; Tyro4; Tyrosine-protein kinase receptor ETK1; Tyrosine-protein kinase TYRO4

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Citations for Mouse EphA3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. IL-26 mediates epidermal growth factor receptor-tyrosine kinase inhibitor resistance through endoplasmic reticulum stress signaling pathway in triple-negative breast cancer cells
    Authors: T Itoh, R Hatano, Y Horimoto, T Yamada, D Song, H Otsuka, Y Shirakawa, S Mastuoka, N Iwao, TM Aune, NH Dang, Y Kaneko, K Okumura, C Morimoto, K Ohnuma
    Cell Death & Disease, 2021-05-21;12(6):520.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  2. BMAL1 Knockdown Leans Epithelial–Mesenchymal Balance toward Epithelial Properties and Decreases the Chemoresistance of Colon Carcinoma Cells
    Authors: Yuan Zhang, Aurore Devocelle, Christophe Desterke, Lucas Eduardo Botelho de Souza, Éva Hadadi, Hervé Acloque et al.
    International Journal of Molecular Sciences
  3. Expression and activation of ephexin is altered after spinal cord injury
    Authors: Odrick R. Rosas, Johnny D. Figueroa, Aranza I. Torrado, Mónica Rivera, José M. Santiago, Franchesca Konig-Toro et al.
    Developmental Neurobiology
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  4. EphA3 null mutants do not demonstrate motor axon guidance defects.
    Authors: Vaidya A, Pniak A, Lemke G, Brown A
    Mol. Cell. Biol., 2003-11-01;23(22):8092-8.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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