Mouse EphA4 Antibody

Catalog # Availability Size / Price Qty
AF641
AF641-SP
Mouse EphA4 Antibody in Data
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Product Details
Citations (7)
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Mouse EphA4 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse EphA4 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human EphA4 is observed, and less than 5% cross-reactivity with recombinant mouse (rm) EphA3, rmEphA6, rmEphA7, and rmEphA8 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse EphA4
Val20-Thr547
Accession # Q03137
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse EphA4 Fc Chimera (Catalog # 641-A4)
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry EphA4 in Embryonic Rat Rib Cartilage Primordium. View Larger

EphA4 in Embryonic Rat Rib Cartilage Primordium. EphA4 was detected in immersion fixed frozen sections of embryonic rat rib cartilage primordium (E15) using 5 µg/mL Goat Anti-Mouse EphA4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF641) overnight at 4 °C. Tissue was stained (red). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EphA4

EphA4, also known as Sek, Sek1, Cek8, Hek8, and Tyro1 (1), is a member of the Eph receptor family which binds members of the ephrin ligand family. There are two classes of receptors, designated A and B. Both the A and B class receptors have an extracellular region consisting of a globular domain, a cysteine-rich domain, and two fibronectin type III domains. This is followed by the transmembrane region and cytoplasmic region. The cytoplasmic region contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxy tail which contains one conserved tyrosine residue. Activation of kinase activity occurs after ligand recognition and binding. EphA4 has been shown to bind ephrin-A5, ephrin-A1, ephrin‑A3, ephrin-A2, ephrin-B2, ephrin-B3, and ephrin-A4 (2, 3). The extracellular domains of mouse and human EphA4 share greater than 95% amino acid identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/ephrin families also appear to play a role in angiogenesis (3).

References
  1. Eph Nomenclature Committee [letter] (1997) Cell 9:403.
  2. Flanagan, J.G. and P. Vanderhaegen (1998) Annu. Rev. Neurosci. 21:309.
  3. Pasquale, E.B. (1997) Curr. Opin. Cell. Biol. 9:608.
Long Name
Eph Receptor A4
Entrez Gene IDs
2043 (Human); 13838 (Mouse)
Alternate Names
Cek8; EC 2.7.10; EC 2.7.10.1; EK8; EPH receptor A4; EphA4; EPH-like kinase 8; ephrin type-A receptor 4; Hek8; receptor protein-tyrosine kinase HEK8; SEK; Sek1; TYRO1 protein tyrosine kinase; Tyro1; Tyrosine-protein kinase receptor SEK; Tyrosine-protein kinase TYRO1

Product Datasheets

Citations for Mouse EphA4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. The developmental and genetic basis of 'clubfoot' in the peroneal muscular atrophy mutant mouse
    Authors: JM Collinson, NO Lindström, C Neves, K Wallace, C Meharg, RH Charles, ZK Ross, AM Fraser, I Mbogo, K Oras, M Nakamoto, S Barker, S Duce, Z Miedzybrod, N Vargesson
    Development, 2018;145(3):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. EphA4 regulates neuroblast and astrocyte organization in a neurogenic niche
    Authors: KL Todd, KL Baker, MB Eastman, FW Kolling, AG Trausch, CE Nelson, JC Conover
    J. Neurosci, 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. alpha2-chimaerin is required for Eph receptor-class-specific spinal motor axon guidance and coordinate activation of antagonistic muscles.
    Authors: Kao T, Nicholl G, Johansen J, Kania A, Beg A
    J Neurosci, 2015;35(6):2344-57.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Ephrin-B2 governs morphogenesis of endolymphatic sac and duct epithelia in the mouse inner ear.
    Authors: Raft S, Andrade L, Shao D, Akiyama H, Henkemeyer M, Wu D
    Dev Biol, 2014;390(1):51-67.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. A novel feedback mechanism by Ephrin-B1/B2 in T-cell activation involves a concentration-dependent switch from costimulation to inhibition.
    Authors: Kawano H, Katayama Y, Minagawa K, Shimoyama M, Henkemeyer M, Matsui T
    Eur. J. Immunol., 2012;42(6):1562-72.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  6. Neural RNA-binding protein Musashi1 controls midline crossing of precerebellar neurons through posttranscriptional regulation of Robo3/Rig-1 expression.
    Authors: Kuwako K, Kakumoto K, Imai T
    Neuron, 2010;67(3):407-21.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  7. Control of hippocampal dendritic spine morphology through ephrin-A3/EphA4 signaling.
    Authors: Murai KK, Nguyen LN, Irie F, Yamaguchi Y, Pasquale EB
    Nat. Neurosci., 2003;6(2):153-60.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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