Mouse EphB4 Antibody

(23 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse EphB4 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse (rm) EphB6 and rmEphA3 is observed and less than 1% cross-reactivity with rmEphA2, rmEphA4, rmEphA6, rmEphA7, rmEphA8, rmEphB2, and rmEphB3 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse EphB4
    Leu16-Ala539
    Accession # P54761
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse EphB4 Fc Chimera (Catalog # 446-B4)
  • Flow Cytometry
    2.5 µg/106 cells
    MCF‑7 human breast cancer cell line
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
EphB4 in Mouse Embryo. EphB4 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Goat Anti-Mouse EphB4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF446) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: EphB4
EphB4, also known as Htk, Myk1, Tyro11, and Mdk2 (1), is a member of the Eph receptor family which binds members of the ephrin ligand family. There are two classes of receptors, designated A and B. Both the A and B class receptors have an extracellular region consisting of a globular domain, a cysteine-rich domain, and two fibronectin type III domains. This is followed by the transmembrane region and cytoplasmic region. The cytoplasmic region contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxy tail which contains one conserved tyrosine residue. Activation of kinase activity occurs after ligand recognition and binding. EphB4 has been shown to bind ephrin‑B2 and ephrin‑B1 (2, 3). The extracellular domains of human and mouse EphB4 share 88% amino acid identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/ephrin families also appear to play a role in angiogenesis (3).
  • References:
    1. Eph Nomenclature Committee [letter] (1997) Cell 90:403.
    2. Flanagan, J.G. and P. Vanderhaeghen (1998) Annu. Rev. Neurosci. 21:309.
    3. Pasquale, E.B. (1997) Curr. Opin. Cell Biol. 9:608.
  • Long Name:
    Eph Receptor B4
  • Entrez Gene IDs:
    2050 (Human); 13846 (Mouse)
  • Alternate Names:
    EC 2.7.10; EC 2.7.10.1; EPH receptor B4; EphB4; ephrin type-B receptor 4; hepatoma transmembrane kinase; Htk; HTKephrin receptor EphB4; Mdk2; Myk1; soluble EPHB4 variant 1; soluble EPHB4 variant 2; soluble EPHB4 variant 3; Tyro11; Tyrosine-protein kinase receptor HTK; Tyrosine-protein kinase TYRO11
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

23 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Histochemical assessment for osteoblastic activity coupled with dysfunctional osteoclasts in c-src deficient mice
    Authors: H Toray, T Hasegawa, N Sakagami, E Tsuchiya, A Kudo, S Zhao, Y Moritani, M Abe, T Yoshida, T Yamamoto, T Yamamoto, K Oda, N Udagawa, PH Luiz de Fr, M Li
    Biomed. Res., 2017;38(2):123-134.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  2. EPHB4 kinase-inactivating mutations cause autosomal dominant lymphatic-related hydrops fetalis
    J Clin Invest, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Fresh
  3. Downregulation of proinflammatory cytokines in HTLV-1-infected T cells by Resveratrol
    J Exp Clin Cancer Res, 2016;35(1):118.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  4. EphrinB2 repression through ZEB2 mediates tumour invasion and anti-angiogenic resistance
    Nat Commun, 2016;7(0):12329.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  5. EphB4 Expressing Stromal Cells Exhibit an Enhanced Capacity for Hematopoietic Stem Cell Maintenance.
    Authors: Nguyen T, Arthur A, Panagopoulos R, Paton S, Hayball J, Zannettino A, Purton L, Matsuo K, Gronthos S
    Stem Cells, 2015;33(9):2838-49.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  6. EphB4 forward signalling regulates lymphatic valve development.
    Authors: Zhang, Gu, Brady, John, Liang, Wei-Chin, Wu, Yan, Henkemeyer, Mark, Yan, Minhong
    Nat Commun, 2015;6(0):6625.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  7. The in vivo effect of prophylactic subchondral bone protection of osteoarthritic synovial membrane in bone-specific Ephb4-overexpressing mice.
    Authors: Valverde-Franco G, Hum D, Matsuo K, Lussier B, Pelletier J, Fahmi H, Kapoor M, Martel-Pelletier J
    Am J Pathol, 2015;185(2):335-46.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  8. Sox17 is required for normal pulmonary vascular morphogenesis.
    Authors: Lange A, Haitchi H, LeCras T, Sridharan A, Xu Y, Wert S, James J, Udell N, Thurner P, Whitsett J
    Dev Biol, 2014;387(1):109-20.
  9. Molecular identification of venous progenitors in the dorsal aorta reveals an aortic origin for the cardinal vein in mammals.
    Authors: Lindskog, Henrik, Kim, Yung Hae, Jelin, Eric B, Kong, Yupeng, Guevara-Gallardo, Salvador, Kim, Tyson N, Wang, Rong A
    Development, 2014;141(5):1120-8.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  10. Single-cell western blotting.
    Authors: Hughes A, Spelke D, Xu Z, Kang C, Schaffer D, Herr A
    Nat Methods, 2014;11(7):749-55.
    Species: Rat
    Sample Type: Cell Lysates
    Application: WB
  11. A novel feedback mechanism by Ephrin-B1/B2 in T-cell activation involves a concentration-dependent switch from costimulation to inhibition.
    Authors: Kawano H, Katayama Y, Minagawa K, Shimoyama M, Henkemeyer M, Matsui T
    Eur. J. Immunol., 2012;42(6):1562-72.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: IP
  12. Eph-B4 prevents venous adaptive remodeling in the adult arterial environment.
    Authors: Muto A, Yi T, Harrison KD, Davalos A, Fancher TT, Ziegler KR, Feigel A, Kondo Y, Nishibe T, Sessa WC, Dardik A
    J. Exp. Med., 2011;208(3):561-75.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  13. EphB-ephrin-B2 interactions are required for thymus migration during organogenesis.
    Authors: Foster KE, Gordon J, Cardenas K
    Proc. Natl. Acad. Sci. U.S.A., 2010;107(30):13414-9.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  14. Direct transcriptional regulation of neuropilin-2 by COUP-TFII modulates multiple steps in murine lymphatic vessel development.
    Authors: Lin FJ, Chen X, Qin J, Hong YK, Tsai MJ, Tsai SY
    J. Clin. Invest., 2010;120(5):1694-707.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  15. Ephrin-B2 controls VEGF-induced angiogenesis and lymphangiogenesis.
    Authors: Wang Y, Nakayama M, Pitulescu ME, Schmidt TS, Bochenek ML, Sakakibara A, Adams S, Davy A, Deutsch U, Luthi U, Barberis A, Benjamin LE, Makinen T, Nobes CD, Adams RH
    Nature, 2010;465(7297):483-6.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  16. Endothelial-specific expression of WNK1 kinase is essential for angiogenesis and heart development in mice.
    Authors: Xie J, Wu T, Xu K, Huang IK, Cleaver O, Huang CL
    Am. J. Pathol., 2009;175(3):1315-27.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  17. Dynamic changes occur in patterns of endometrial EFNB2/EPHB4 expression during the period of spiral arterial modification in mice.
    Authors: Zhang J, Dong H, Wang B, Zhu S, Croy BA
    Biol. Reprod., 2008;79(3):450-8.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  18. EphrinB2 regulation by PTH and PTHrP revealed by molecular profiling in differentiating osteoblasts.
    Authors: Allan EH, Hausler KD, Wei T, Gooi JH, Quinn JM, Crimeen-Irwin B, Pompolo S, Sims NA, Gillespie MT, Onyia JE, Martin TJ
    J. Bone Miner. Res., 2008;23(8):1170-81.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  19. Artery and vein size is balanced by Notch and ephrin B2/EphB4 during angiogenesis.
    Authors: Kim YH, Hu H, Guevara-Gallardo S, Lam MT, Fong SY, Wang RA
    Development, 2008;135(22):3755-64.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Fresh
  20. EphB2 and ephrin-B1 expressed in the adult kidney regulate the cytoarchitecture of medullary tubule cells through Rho family GTPases.
    Authors: Ogawa K, Wada H, Okada N, Harada I, Nakajima T, Pasquale EB, Tsuyama S
    J. Cell. Sci., 2006;119(0):559-70.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  21. The EphB4 receptor-tyrosine kinase promotes the migration of melanoma cells through Rho-mediated actin cytoskeleton reorganization.
    Authors: Yang NY, Pasquale EB, Owen LB, Ethell IM
    J. Biol. Chem., 2006;281(43):32574-86.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: IP
  22. Inhibition of tumor growth and angiogenesis by soluble EphB4.
    Authors: Martiny-Baron G, Korff T, Schaffner F, Esser N, Eggstein S, Marme D, Augustin HG
    Neoplasia, 2004;6(3):248-57.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  23. Forward EphB4 signaling in endothelial cells controls cellular repulsion and segregation from ephrinB2 positive cells.
    Authors: Fuller T, Korff T, Kilian A, Dandekar G, Augustin HG
    J. Cell. Sci., 2003;116(0):2461-70.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
Expand to show all 23 Citations
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