Mouse Ephrin-B1 Antibody

(13 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Ephrin-B1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) Ephrin-A3, rhEphrin-A4, recombinant mouse (rm) Ephrin-A1, rmEphrin-B2, rhEphrin-A5, rhEphrin-B3, rmEphrin-A2, rmEphrin‑A4, and recombinant zebrafish Ephrin‑B2 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Ephrin-B1
    Lys30-Ser229
    Accession # AAA53231
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.25 µg/mL
    See below
  • Simple Western
    25 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse Ephrin‑B1 by Western Blot. Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse Ephrin‑B1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF473) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Ephrin‑B1 at approximately 45-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
Ephrin-B1 in Mouse Kidney. Ephrin-B1 was detected in immersion fixed frozen sections of mouse kidney using Goat Anti-Mouse Ephrin-B1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF473) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Detection of Mouse Ephrin‑B1 by Simple WesternTM. Simple Western lane view shows lysates of mouse lung tissue and mouse spleen tissue, loaded at 0.2 mg/mL. A specific band was detected for Ephrin‑B1 at approximately 65 kDa (as indicated) using 25 µg/mL of Goat Anti-Mouse Ephrin‑B1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF473) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Ephrin-B1

Ephrin-B1, also known as LERK-2, ELK-L, EFL-3, Cek5-L, and STRA-1 (1), is a member of the ephrin ligand family which binds members of the Eph receptor family. All ligands share a conserved extracellular sequence, which most likely corresponds to the receptor binding domain. This conserved sequence consists of approximately 125 amino acids and includes four invariant cysteines. The B-class ligands are transmembrane proteins which can become tyrosine phosphorylated upon receptor ligation. The cytoplasmic domains are approximately 80 amino acids long and are highly conserved, especially the last 33 amino acids. Several signaling molecules have been shown to interact with the cytoplasmic region, although specific signaling roles have yet to be elucidated. Ephrin-B1 has been shown to bind EphA3, EphB1, EphB2, EphB3, and EphB4 (2, 3). The extracellular domains of human and mouse Ephrin-B1 share 94% amino acid identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. Soluble monomeric ligands bind the receptor but do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/ephrin families also appear to play a role in angiogenesis (3).

  • References:
    1. Eph Nomenclature Committee [letter] (1997) Cell 90:403.
    2. Flanagan, J.G. and P. Vanderhaeghen (1998) Annu. Rev. Neurosci. 21:309.
    3. Pasquale, E.B. (1997) Curr. Opin. Cell Biol. 9:608.
  • Entrez Gene IDs:
    1947 (Human); 13641 (Mouse); 25186 (Rat)
  • Alternate Names:
    Cek5-L; EFL-3; EFNB1; ELK-L; EphrinB1; Ephrin-B1; LERK-2; STRA-1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Multiple mouse models of primary lymphedema exhibit distinct defects in lymphovenous valve development.
    Authors: Geng X, Cha B, Mahamud M, Lim K, Silasi-Mansat R, Uddin M, Miura N, Xia L, Simon A, Engel J, Chen H, Lupu F, Srinivasan R
    Dev Biol, 2016;409(1):218-33.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC-OCT embedded
  2. GluA2 trafficking is involved in apoptosis of retinal ganglion cells induced by activation of EphB/EphrinB reverse signaling in a rat chronic ocular hypertension model.
    Authors: Dong L, Gao F, Wang X, Miao Y, Wang S, Wu Y, Li F, Wu J, Cheng X, Sun X, Yang X, Wang Z
    J Neurosci, 2015;35(13):5409-21.
    Species: Rat
    Sample Type: Tissue Homogenates
    Application: WB
  3. Tissue-Specific Effects of Reduced beta-catenin Expression on Adenomatous Polyposis Coli Mutation-Instigated Tumorigenesis in Mouse Colon and Ovarian Epithelium.
    Authors: Feng Y, Sakamoto N, Wu R, Liu J, Wiese A, Green M, Green M, Akyol A, Roy B, Zhai Y, Cho K, Fearon E
    PLoS Genet, 2015;11(11):e1005638.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - paraffin
  4. Cortical abnormalities and non-spatial learning deficits in a mouse model of CranioFrontoNasal syndrome.
    Authors: Arvanitis D, Behar A, Drougard A, Roullet P, Davy A
    PLoS ONE, 2014;9(2):e88325.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  5. Ephrin-B1 controls the columnar distribution of cortical pyramidal neurons by restricting their tangential migration.
    Authors: Dimidschstein J, Passante L, Dufour A, van den Ameele J, Tiberi L, Hrechdakian T, Adams R, Klein R, Lie D, Jossin Y, Vanderhaeghen P
    Neuron, 2013;79(6):1123-35.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: IP
  6. Possible role of Efnb1 protein, a ligand of Eph receptor tyrosine kinases, in modulating blood pressure.
    Authors: Wu Z, Luo H, Thorin E, Tremblay J, Peng J, Lavoie JL, Wang Y, Qi S, Wu T, Wu J
    J. Biol. Chem., 2012;287(19):15557-69.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  7. Ephrin-B reverse signaling promotes structural and functional synaptic maturation in vivo.
    Authors: Lim BK, Matsuda N, Poo MM
    Nat. Neurosci., 2008;11(2):160-9.
    Species: Xenopus
    Sample Type: Tissue Homogenates
    Application: IP
  8. Inhibition of inflammatory lymphangiogenesis by integrin alpha5 blockade.
    Authors: Dietrich T, Onderka J, Bock F, Kruse FE, Vossmeyer D, Stragies R, Zahn G, Cursiefen C
    Am. J. Pathol., 2007;171(1):361-72.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  9. Binding of ras to phosphoinositide 3-kinase p110alpha is required for ras-driven tumorigenesis in mice.
    Authors: Gupta S, Ramjaun AR, Haiko P, Wang Y, Warne PH, Nicke B, Nye E, Stamp G, Alitalo K, Downward J
    Cell, 2007;129(5):957-68.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Fresh
  10. Intracellular and trans-synaptic regulation of glutamatergic synaptogenesis by EphB receptors.
    Authors: Kayser MS, McClelland AC, Hughes EG, Dalva MB
    J. Neurosci., 2006;26(47):12152-64.
    Species: Rat
    Sample Type: Whole Cells
    Application: ICC
  11. EphB2 and ephrin-B1 expressed in the adult kidney regulate the cytoarchitecture of medullary tubule cells through Rho family GTPases.
    Authors: Ogawa K, Wada H, Okada N, Harada I, Nakajima T, Pasquale EB, Tsuyama S
    J. Cell. Sci., 2006;119(0):559-70.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  12. The EphB4 receptor-tyrosine kinase promotes the migration of melanoma cells through Rho-mediated actin cytoskeleton reorganization.
    Authors: Yang NY, Pasquale EB, Owen LB, Ethell IM
    J. Biol. Chem., 2006;281(43):32574-86.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  13. EphrinB1 is essential in T-cell-T-cell co-operation during T-cell activation.
    Authors: Yu G, Luo H, Wu Y, Wu J
    J. Biol. Chem., 2004;279(53):55531-9.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
Expand to show all 13 Citations
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