Mouse HAI-2 Ectodomain Antibody

Catalog # Availability Size / Price Qty
AF1107
AF1107-SP
Product Details
Citations (3)
FAQs
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Mouse HAI-2 Ectodomain Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse HAI-2 Ectodomain in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human HAI-2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse HAI-2B
Arg30-Lys140
Accession # NP_001076017
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse HAI‑2 Ectodomain
Immunohistochemistry
5-15 µg/mL
Perfusion fixed frozen sections of mouse liver

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HAI-2

Two alternatively spliced forms of HAI-2 are found in human tissues. HAI-2A, the full-length molecule and also known as placental bikunin, is a major form expressed in human tissues. HAI-2A consists of two Kunitz domains, and a C-terminal transmembrane domain. Both Kunitz domains can function as inhibitors independent of each other. In addition to HGF activator and trypsin, HAI-2A strongly inhibits plasmin, tissue and plasma kallikreins, and factor XIa.

Three alternatively spliced forms of HAI-2 have been found in mouse tissues. HAI-2A is a minor form expressed in mouse tissues. HAI-2B, with residues 37 through 93 spliced out and thus lack of the first Kunitz domain, is a major form in mouse tissues. HAI-2B is an effective inhibitor of both HGF activator and trypsin.

Long Name
HGF Activator Inhibitor Type 2
Entrez Gene IDs
10653 (Human); 20733 (Mouse)
Alternate Names
HAI2; HAI-2; HAI-2DIAR3; HAI2FLJ45571; Hepatocyte growth factor activator inhibitor type 2; Kop; kunitz-type protease inhibitor 2; PB; Placental bikunin; serine peptidase inhibitor, Kunitz type, 2; serine protease inhibitor, Kunitz type, 2

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Citations for Mouse HAI-2 Ectodomain Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Matriptase Cleaves EpCAM and TROP2 in Keratinocytes, Destabilizing Both Proteins and Associated Claudins
    Authors: CJ Wu, M Lu, X Feng, G Nakato, MC Udey
    Cells, 2020-04-21;9(4):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. Matriptase promotes inflammatory cell accumulation and progression of established epidermal tumors.
    Authors: Sales K, Friis S, Abusleme L, Moutsopoulos N, Bugge T
    Oncogene, 2014-12-08;34(35):4664-72.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. Potent Inhibition and Global Co-localization Implicate the Transmembrane Kunitz-type Serine Protease Inhibitor Hepatocyte Growth Factor Activator Inhibitor-2 in the Regulation of Epithelial Matriptase Activity.
    Authors: Szabo R, Hobson JP, List K, Molinolo A, Lin CY, Bugge TH
    J. Biol. Chem., 2008-08-19;283(43):29495-504.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Western Blot

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