Mouse HSF1 Antibody

Catalog # Availability Size / Price Qty
AF7130
AF7130-SP
HSF1 in Mouse Testes.
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Mouse HSF1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse HSF1 in direct ELISAs. In direct ELISAs, approximately 6% cross-reactivity with recombinant mouse HSF4 is observed and less than 1% cross-reactivity with recombinant human HSF2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse HSF1
Thr124-Tyr247
Accession # P38532
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry HSF1 antibody in Mouse Testes by Immunohistochemistry (IHC-Fr). View Larger

HSF1 in Mouse Testes. HSF1 was detected in perfusion fixed frozen sections of mouse testes using Goat Anti-Mouse HSF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7130) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to the nuclei of spermatocytes. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HSF1

HSF1 (Heat Shock Factor 1; also HSTF 1) is a 67-75 kDa member of the HSF family of proteins. It is constitutively expressed and functions as a transcriptional activator under heat shock conditions. Normally, it exists as an inactive, monomeric member of the HSP multichaperone complex. Following stress, it dissociates from the complex, homotrimerizes, undergoes multiple phosphorylations, and translocates to the nucleus. Nuclear HSF1 is SUMOylated on Lys298 and binds to heat shock elements in select genes such as HSP70. Mouse HSF1 is 525 amino acids (aa) in length. It contains a DNA binding domain (aa 15-120), two hydrophobic oligomerization regions (aa 130-203 and 380-405), a regulatory domain (aa 221-310), and a transactivation domain (aa 367-525). There are at least 13 potential Ser/Thr phosphorylation sites. When highly phosphorylated, HSF1 appears to run at about 82-85 kDa in SDS-PAGE; when SUMOylated, it runs anomalously at around 170 kDa in SDS-PAGE. One splice variant exists that shows a deletion of aa 413-434. Over aa 124-247, mouse HSF1 shares 94% and 99% aa identity with human and rat HSF1, respectively.

Long Name
Heat Shock Transcription Factor 1
Entrez Gene IDs
3297 (Human); 154991 (Mouse); 79245 (Rat)
Alternate Names
heat shock factor protein 1; heat shock transcription factor 1HSTF1HSF 1; HSF1; HSTF 1; HSTF1

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