Mouse IGFALS/ALS Antibody

Catalog # Availability Size / Price Qty
Detection of Mouse IGFALS/ALS by Western Blot.
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Product Details
Citations (3)
Supplemental Products

Mouse IGFALS/ALS Antibody Summary

Species Reactivity
Detects mouse IGFALS/ALS in direct ELISAs and Western blots.
Polyclonal Goat IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant mouse IGFALS/ALS
Accession # P70389
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse IGFALS/ALS antibody by Western Blot. View Larger

Detection of Mouse IGFALS/ALS by Western Blot. Western blot shows lysates of mouse spleen tissue, mouse liver tissue, and mouse bone marrow. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse IGFALS/ALS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1436) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for IGFALS/ALS at approximately 80-90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGFALS/ALS

ALS (Acid labile subunit) is an 84-86 kDa glycoprotein member of the leucine rich repeat superfamily of molecules.  It is secreted by hepatocytes in response to growth hormone stimulation, and forms a 140-150 kDa ternary complex with IGFBP3 (or IGFBP5) and IGF-I.  This complex increases the half-life of IGF-I and retains it in the circulation for use in multiple tissues.  Mature mouse ALS is 580 amino acids (aa) in length (aa 24-603).  It contains twenty-one 20-23 aa LRRs that participate in protein-protein interactions.  There are two potential isoform variants that show N-terminal extensions.  One contains a 66 aa substitution for aa 1-5, while another possesses an 89 aa substitution again for aa 1-5.  Over aa 28-603, mouse ALS shares 79% and 93% aa identity with human and rat ALS, respectively.        

Long Name
IGF-binding Protein Acid Labile Subunit
Entrez Gene IDs
3483 (Human); 16005 (Mouse)
Alternate Names

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Citations for Mouse IGFALS/ALS Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Growth Hormone Control of Hepatic Lipid Metabolism
    Authors: Zhongbo Liu, Jose Cordoba-Chacon, Rhonda D. Kineman, Bruce N. Cronstein, Radhika Muzumdar, Zhenwei Gong et al.
  2. Longevity effect of IGF-1R(+/-) mutation depends on genetic background-specific receptor activation
    Authors: Jie Xu, Géraldine Gontier, Zayna Chaker, Philippe Lacube, Joëlle Dupont, Martin Holzenberger
    Aging Cell
  3. Early postnatal nutrition determines somatotropic function in mice.
    Authors: Kappeler L, De Magalhaes Filho C, Leneuve P, Xu J, Brunel N, Chatziantoniou C, Le Bouc Y, Holzenberger M
    Endocrinology, 2008-09-18;150(1):314-23.
    Species: Mouse
    Sample Types: Plasma
    Applications: Western Blot


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