Mouse IgG HRP-conjugated Antibody

(26 citations)
(4 Reviews)
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse IgG1, IgG2A, IgG2B, and IgG3 heavy and light chains in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross‑reactivity with human IgG and rabbit IgG is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse IgG
  • Formulation
    Sodium Phosphate and Proclin 300.
  • Label
    HRP (Horseradish Peroxidase)
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot

    Use at a 1:1000 dilution.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse IgG Primary Antibody byWestern Blot Westernblot shows detection of Mouse Anti-Human/Mouse/Rat STAT3 Monoclonal Antibody
(Catalog #MAB1799)using a 1:1000 dilution of HRP-conjugated Anti-Mouse IgG Secondary Antibody
(Catalog # HAF007).  This experiment wasconducted under reducing conditions and using ImmunoblotBuffer Group 1.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Do not freeze.
    • 6 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

  • Long Name:
    Immunoglobulin G
  • Alternate Names:
    IgG; immunoglobulin heavy constant gamma 1 (G1m marker)
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

26 Citations: Showing 1 - 10
Filter your results:

Species
Applications
Sample Type
  1. Fasting selectively blocks development of acute lymphoblastic leukemia via leptin-receptor upregulation
    Nat. Med, 2016;0(0):.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  2. Cellular Stress and p53-Associated Apoptosis by Juniperus communis L. Berry Extract Treatment in the Human SH-SY5Y Neuroblastoma Cells
    Int J Mol Sci, 2016;17(7):.
    Application: WB
  3. The GTPase ARF6 Controls ROS Production to Mediate Angiotensin II-Induced Vascular Smooth Muscle Cell Proliferation.
    Authors: Bourmoum M, Charles R, Claing A
    PLoS ONE, 2016;11(1):e0148097.
  4. microRNA-155, induced by interleukin-1ss, represses the expression of microphthalmia-associated transcription factor (MITF-M) in melanoma cells.
    Authors: Arts N, Cane S, Hennequart M, Lamy J, Bommer G, Van den Eynde B, De Plaen E
    PLoS ONE, 2015;10(4):e0122517.
    Species: Human
    Sample Type: Cell Lysates
    Application: Neut
  5. MicroRNA-7 Promotes Glycolysis to Protect against 1-Methyl-4-phenylpyridinium-induced Cell Death.
    Authors: Chaudhuri A, Kabaria S, Choi D, Mouradian M, Junn E
    J Biol Chem, 2015;290(19):12425-34.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  6. Decidualization and syndecan-1 knock down sensitize endometrial stromal cells to apoptosis induced by embryonic stimuli.
    Authors: Boeddeker S, Baston-Buest D, Fehm T, Kruessel J, Hess A
    PLoS ONE, 2015;10(4):e0121103.
    Species: Human
    Sample Type: Cell Lysates
    Application: Bioassay
  7. Mucosal pemphigus vulgaris anti-Dsg3 IgG is pathogenic to the oral mucosa of humanized Dsg3 mice.
    Authors: Culton D, McCray S, Park M, Roberts J, Li N, Zedek D, Anhalt G, Cowley D, Liu Z, Diaz L
    J Invest Dermatol, 2015;135(6):1590-7.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  8. Identification and characterization of PERK activators by phenotypic screening and their effects on NRF2 activation.
    Authors: Xie W, Pariollaud M, Wixted W, Chitnis N, Fornwald J, Truong M, Pao C, Liu Y, Ames R, Callahan J, Solari R, Sanchez Y, Diehl A, Li H
    PLoS ONE, 2015;10(3):e0119738.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  9. Symmorphosis through dietary regulation: a combinatorial role for proteolysis, autophagy and protein synthesis in normalising muscle metabolism and function of hypertrophic mice after acute starvation.
    Authors: Collins-Hooper H, Sartori R, Giallourou N, Matsakas A, Mitchell R, Makarenkova H, Flasskamp H, Macharia R, Ray S, Swann J, Sandri M, Patel K
    PLoS ONE, 2015;10(3):e0120524.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  10. Up-regulation of VEGF by retinoic acid during hyperoxia prevents retinal neovascularization and retinopathy.
    Authors: Wang L, Shi P, Xu Z, Li J, Xie Y, Mitton K, Drenser K, Yan Q
    Invest Ophthalmol Vis Sci, 2014;55(7):4276-87.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  11. ADAMTS1 inhibits lymphangiogenesis by attenuating phosphorylation of the lymphatic endothelial cell-specific VEGF receptor.
    Authors: Inagaki J, Takahashi K, Ogawa H, Asano K, Faruk Hatipoglu O, Cilek M, Obika M, Ohtsuki T, Hofmann M, Kusachi S, Ninomiya Y, Hirohata S
    Exp Cell Res, 2014;323(2):263-75.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  12. HIV and HCV activate the inflammasome in monocytes and macrophages via endosomal Toll-like receptors without induction of type 1 interferon.
    Authors: Chattergoon M, Latanich R, Quinn J, Winter M, Buckheit R, Blankson J, Pardoll D, Cox A
    PLoS Pathog, 2014;10(5):e1004082.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  13. Exploring codon optimization and response surface methodology to express biologically active transmembrane RANKL in E. coli.
    Authors: Maharjan S, Singh B, Bok J, Kim J, Jiang T, Cho C, Kang S, Choi Y
    PLoS ONE, 2014;9(5):e96259.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: WB
  14. Proangiogenic tumor proteins as potential predictive or prognostic biomarkers for bevacizumab therapy in metastatic colorectal cancer.
    Authors: Bruhn M, Townsend A, Khoon Lee C, Shivasami A, Price T, Wrin J, Arentz G, Tebbutt N, Hocking C, Cunningham D, Hardingham J
    Int J Cancer, 2014;135(3):731-41.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: WB
  15. Ligand-independent canonical Wnt activity in canine mammary tumor cell lines associated with aberrant LEF1 expression.
    Authors: Gracanin A, Timmermans-Sprang E, van Wolferen M, Rao N, Grizelj J, Vince S, Hellmen E, Mol J
    PLoS ONE, 2014;9(6):e98698.
    Species: N/A
    Sample Type: N/A
    Application: WB
  16. MicroRNA-7 protects against 1-methyl-4-phenylpyridinium-induced cell death by targeting RelA.
    Authors: Choi D, Chae Y, Kabaria S, Chaudhuri A, Jain M, Li H, Mouradian M, Junn E
    J Neurosci, 2014;34(38):12725-37.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  17. Cell proliferation and apoptosis in rat mammary glands following combinational exposure to bisphenol A and genistein.
    Authors: Wang J, Jenkins S, Lamartiniere C
    BMC Cancer, 2014;14(0):379.
    Species: Rat
    Sample Type: Cell Lysates
    Application: WB
  18. Iron-responsive miR-485-3p regulates cellular iron homeostasis by targeting ferroportin.
    Authors: Sangokoya, Carolyn, Doss, Jennifer, Chi, Jen-Tsan
    PLoS Genet, 2013;9(4):e1003408.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  19. Proprotein convertase subtilisin/kexin type 7 (PCSK7) is essential for the zebrafish development and bioavailability of transforming growth factor beta1a (TGFbeta1a).
    Authors: Turpeinen H, Oksanen A, Kivinen V, Kukkurainen S, Uusimaki A, Ramet M, Parikka M, Hytonen V, Nykter M, Pesu M
    J Biol Chem, 2013;288(51):36610-23.
    Species: Human
    Sample Type:
    Application: WB
  20. Characterization of proprotein convertase subtilisin/kexin type 9 (PCSK9) trafficking reveals a novel lysosomal targeting mechanism via amyloid precursor-like protein 2 (APLP2).
    Authors: DeVay, Rachel M, Shelton, David L, Liang, Hong
    J Biol Chem, 2013;288(15):10805-18.
    Species: Human
    Sample Type: Cell Lysates
    Application: ELISA Development
  21. Surface molecule CD229 as a novel target for the diagnosis and treatment of multiple myeloma.
    Authors: Atanackovic D, Panse J, Hildebrandt Y, Jadczak A, Kobold S, Cao Y, Templin J, Meyer S, Reinhard H, Bartels K, Lajmi N, Zander AR, Marx AH, Luetkens T, Bokemeyer C, Kroger N
    Haematologica, 2011;0(0):.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  22. Anti-inflammatory activity of nanocrystalline silver-derived solutions in porcine contact dermatitis.
    Authors: Nadworny PL, Wang J, Tredget EE
    J Inflamm (Lond), 2010;7(0):13.
    Species: Porcine
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  23. BAMBI is expressed in endothelial cells and is regulated by lysosomal/autolysosomal degradation.
    Authors: Xavier S, Gilbert V, Rastaldi MP, Krick S, Kollins D, Reddy A, Bottinger E, Cohen CD, Schlondorff D
    PLoS ONE, 2010;5(9):e12995.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  24. Comparison of proteomic and transcriptomic profiles in the bronchial airway epithelium of current and never smokers.
    Authors: Steiling K, Kadar AY, Bergerat A
    PLoS ONE, 2009;4(4):e5043.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  25. Mannan-binding lectin-associated serine protease 3 cleaves synthetic peptides and insulin-like growth factor-binding protein 5.
    Authors: Cortesio CL, Jiang W
    Arch. Biochem. Biophys., 2006;449(1):164-70.
    Species: N/A
    Sample Type: N/A
    Application: WB
  26. Characterization of cathepsin L secreted by Sf21 insect cells.
    Authors: Johnson GD, Jiang W
    Arch. Biochem. Biophys., 2005;444(1):7-14.
    Species: N/A
    Sample Type: N/A
    Application: WB
Expand to show all 26 Citations
Secondary Antibodies
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Mouse IgG HRP-conjugated Antibody

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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

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Mouse IgG Antibody

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Goat Anti-Mouse IgG Biotinylated Antibody

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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

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Average Rating: 4.3 (Based on 4 reviews)

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Read what people are saying who have used Mouse IgG HRP-conjugated Antibody.

We have 3 reviews tested in 3 species: E. coli, Goat, Mouse.
We have 4 reviews tested in 2 applications: ELISA, Western Blot.

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Images Ratings Applications Species Reviewed By Date Details
  Excellent
 ELISA Goat Anonymous 10/20/2017
Show

Summary

ApplicationELISA
Sample Testedantibody
SpeciesGoat
Western Blot Mouse IgG HRP-conjugated Antibody HAF007
Enlarge
Excellent
 WB Anonymous 06/28/2017
Show
Image Details
Western Blot Mouse IgG HRP-conjugated Antibody HAF007
Western Blot: Mouse IgG HRP-conjugated Antibody [HAF007]

Summary

ApplicationWestern Blot
Sample TestedCell Lysates
Western Blot Mouse IgG Horseradish Peroxidase-conjugated Antibody HAF007
Enlarge
Very Good
 WB Mouse Anonymous 02/22/2016
Show
Image Details
Western Blot Mouse IgG Horseradish Peroxidase-conjugated Antibody HAF007
Western Blot: Mouse IgG Horseradish Peroxidase-conjugated Antibody [HAF007]

Summary

ApplicationWestern Blot
Sample TestedAdipose tissue,Small intestine
SpeciesMouse
Western Blot Mouse IgG Horseradish Peroxidase-conjugated Antibody HAF007
Enlarge
Average
 WB E. coli Anonymous 02/22/2016
Show
Image Details
Western Blot Mouse IgG Horseradish Peroxidase-conjugated Antibody HAF007
Western Blot: Mouse IgG Horseradish Peroxidase-conjugated Antibody [HAF007]

Summary

ApplicationWestern Blot
Sample TestedCell Lysates
SpeciesE. coli

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