Mouse IgG HRP-conjugated Antibody

Name: Mouse IgG HRP-conjugated Antibody
Brand: R&D Systems
Rating: 4.6 (7 reviews)

(33 citations)

(7 Reviews)

Datasheet / CoA / SDS

Western Blot

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Product Details

Citations (33)

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Supplemental Products

Reviews

Species Reactivity

Mouse
Specificity

Detects mouse IgG₁, IgG_2A, IgG_2B, and IgG₃ heavy and light chains in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross‑reactivity with human IgG and rabbit IgG is observed.
Source

Polyclonal Goat IgG
Purification

Antigen Affinity-purified
Immunogen

Mouse IgG
Formulation

Sodium Phosphate and Proclin 300.
Label

HRP (Horseradish Peroxidase)

Product Datasheets

Product Datasheet

COA

Certificate of Analysis Lookup

Certificate of Analysis Request Form

SDS

Applications

Recommended
Concentration

Sample

Western Blot

Use at a 1:1000 dilution.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot

click image to view larger

Detection of Mouse IgG Primary Antibody byWestern Blot Westernblot shows detection of Mouse Anti-Human/Mouse/Rat STAT3 Monoclonal Antibody
(Catalog #MAB1799)using a 1:1000 dilution of HRP-conjugated Anti-Mouse IgG Secondary Antibody
(Catalog # HAF007). This experiment wasconducted under reducing conditions and using ImmunoblotBuffer Group 1.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Do not freeze.
- 6 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name:

Immunoglobulin G

Alternate Names:

IgG; immunoglobulin heavy constant gamma 1 (G1m marker)

Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

33 Citations: Showing 1 - 10
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Species	Applications	Sample Type
Allopregnanolone alters follicular and luteal dynamics during the estrous cycle Authors: JA Asensio, ARR Cáceres, LT Pelegrina, MLÁ Sanhueza, L Scotti, F Parborell, MR Laconi Reprod. Biol. Endocrinol., 2018;16(1):35. Species: Rat Sample Type: Whole Tissue Application: IHC AAV1.NT-3 gene therapy increases muscle fiber diameter through activation of mTOR pathway and metabolic remodeling in a CMT mouse model Authors: ME Yalvac, J Amornvit, L Chen, KM Shontz, S Lewis, Z Sahenk Gene Ther., 2018;0(0):. Species: Mouse Sample Type: Application: WB Key Proteins Involved in Spheroid Formation and Angiogenesis in Endothelial Cells After Long-Term Exposure to Simulated Microgravity Authors: A Dittrich, D Grimm, J Sahana, J Bauer, M Krüger, M Infanger, NE Magnusson Cell. Physiol. Biochem., 2018;45(2):429-445. Species: Human Sample Type: Cell Lysates Application: WB Low-dose paclitaxel downregulates MYC proto-oncogene bHLH transcription factor expression in colorectal carcinoma cells Authors: W Li, W Zhu, C Lv, H Qu, K Xu, H Li, H Li, Y Du, G Liu, Y Wang, HJ Wei, HY Zhao Oncol Lett, 2018;15(2):1881-1887. Species: Human Sample Type: Cell Lysates Application: WB Parthenolide reduces gene transcription of prosurvival mediators in U937 cells Authors: S Mohammadi, M Zahedpanah, M Nikbakht, M Shaiegan, G Hamidollah, M Nikugoftar, B Rahmani, D Hamedi Asl Exp. Oncol., 2017;39(1):30-35. Species: Mouse Sample Type: Application: WB Inflammatory Properties and Adjuvant Potential of Synthetic Glycolipids Homologous to Mycolate Esters of the Cell Wall of Mycobacterium tuberculosis Authors: Marta Romano J Innate Immun, 2016;0(0):. Application: Secondary antibody The GTPase ARF6 Controls ROS Production to Mediate Angiotensin II-Induced Vascular Smooth Muscle Cell Proliferation. Authors: Bourmoum M, Charles R, Claing A PLoS ONE, 2016;11(1):e0148097. Gamma secretase inhibitor impairs epithelial-to-mesenchymal transition induced by TGF-? in ovarian tumor cell lines Authors: M C Pazos Mol. Cell. Endocrinol, 2016;440(0):125-137. Species: Mouse Sample Type: Antibody Application: WB Fasting selectively blocks development of acute lymphoblastic leukemia via leptin-receptor upregulation Nat. Med, 2016;0(0):. Species: Mouse Sample Type: Cell Lysates Application: WB Cellular Stress and p53-Associated Apoptosis by Juniperus communis L. Berry Extract Treatment in the Human SH-SY5Y Neuroblastoma Cells Int J Mol Sci, 2016;17(7):. Application: WB microRNA-155, induced by interleukin-1ss, represses the expression of microphthalmia-associated transcription factor (MITF-M) in melanoma cells. Authors: Arts N, Cane S, Hennequart M, Lamy J, Bommer G, Van den Eynde B, De Plaen E PLoS ONE, 2015;10(4):e0122517. Species: Human Sample Type: Cell Lysates Application: Neut MicroRNA-7 Promotes Glycolysis to Protect against 1-Methyl-4-phenylpyridinium-induced Cell Death. Authors: Chaudhuri A, Kabaria S, Choi D, Mouradian M, Junn E J Biol Chem, 2015;290(19):12425-34. Species: Human Sample Type: Cell Lysates Application: WB Decidualization and syndecan-1 knock down sensitize endometrial stromal cells to apoptosis induced by embryonic stimuli. Authors: Boeddeker S, Baston-Buest D, Fehm T, Kruessel J, Hess A PLoS ONE, 2015;10(4):e0121103. Species: Human Sample Type: Cell Lysates Application: Bioassay Symmorphosis through dietary regulation: a combinatorial role for proteolysis, autophagy and protein synthesis in normalising muscle metabolism and function of hypertrophic mice after acute starvation. Authors: Collins-Hooper H, Sartori R, Giallourou N, Matsakas A, Mitchell R, Makarenkova H, Flasskamp H, Macharia R, Ray S, Swann J, Sandri M, Patel K PLoS ONE, 2015;10(3):e0120524. Species: Mouse Sample Type: Tissue Homogenates Application: WB Mucosal pemphigus vulgaris anti-Dsg3 IgG is pathogenic to the oral mucosa of humanized Dsg3 mice. Authors: Culton D, McCray S, Park M, Roberts J, Li N, Zedek D, Anhalt G, Cowley D, Liu Z, Diaz L J Invest Dermatol, 2015;135(6):1590-7. Species: Mouse Sample Type: Tissue Homogenates Application: WB Identification and characterization of PERK activators by phenotypic screening and their effects on NRF2 activation. Authors: Xie W, Pariollaud M, Wixted W, Chitnis N, Fornwald J, Truong M, Pao C, Liu Y, Ames R, Callahan J, Solari R, Sanchez Y, Diehl A, Li H PLoS ONE, 2015;10(3):e0119738. Species: Mouse Sample Type: Cell Lysates Application: WB ADAMTS1 inhibits lymphangiogenesis by attenuating phosphorylation of the lymphatic endothelial cell-specific VEGF receptor. Authors: Inagaki J, Takahashi K, Ogawa H, Asano K, Faruk Hatipoglu O, Cilek M, Obika M, Ohtsuki T, Hofmann M, Kusachi S, Ninomiya Y, Hirohata S Exp Cell Res, 2014;323(2):263-75. Species: Human Sample Type: Cell Lysates Application: WB MicroRNA-7 protects against 1-methyl-4-phenylpyridinium-induced cell death by targeting RelA. Authors: Choi D, Chae Y, Kabaria S, Chaudhuri A, Jain M, Li H, Mouradian M, Junn E J Neurosci, 2014;34(38):12725-37. Species: Human Sample Type: Cell Lysates Application: WB Ligand-independent canonical Wnt activity in canine mammary tumor cell lines associated with aberrant LEF1 expression. Authors: Gracanin A, Timmermans-Sprang E, van Wolferen M, Rao N, Grizelj J, Vince S, Hellmen E, Mol J PLoS ONE, 2014;9(6):e98698. Species: N/A Sample Type: N/A Application: WB Cell proliferation and apoptosis in rat mammary glands following combinational exposure to bisphenol A and genistein. Authors: Wang J, Jenkins S, Lamartiniere C BMC Cancer, 2014;14(0):379. Species: Rat Sample Type: Cell Lysates Application: WB HIV and HCV activate the inflammasome in monocytes and macrophages via endosomal Toll-like receptors without induction of type 1 interferon. Authors: Chattergoon M, Latanich R, Quinn J, Winter M, Buckheit R, Blankson J, Pardoll D, Cox A PLoS Pathog, 2014;10(5):e1004082. Species: Human Sample Type: Cell Lysates Application: WB Exploring codon optimization and response surface methodology to express biologically active transmembrane RANKL in E. coli. Authors: Maharjan S, Singh B, Bok J, Kim J, Jiang T, Cho C, Kang S, Choi Y PLoS ONE, 2014;9(5):e96259. Species: Mouse Sample Type: Cell Culture Supernates Application: WB Up-regulation of VEGF by retinoic acid during hyperoxia prevents retinal neovascularization and retinopathy. Authors: Wang L, Shi P, Xu Z, Li J, Xie Y, Mitton K, Drenser K, Yan Q Invest Ophthalmol Vis Sci, 2014;55(7):4276-87. Species: Mouse Sample Type: Tissue Homogenates Application: WB Proangiogenic tumor proteins as potential predictive or prognostic biomarkers for bevacizumab therapy in metastatic colorectal cancer. Authors: Bruhn M, Townsend A, Khoon Lee C, Shivasami A, Price T, Wrin J, Arentz G, Tebbutt N, Hocking C, Cunningham D, Hardingham J Int J Cancer, 2014;135(3):731-41. Species: Human Sample Type: Tissue Homogenates Application: WB Iron-responsive miR-485-3p regulates cellular iron homeostasis by targeting ferroportin. Authors: Sangokoya, Carolyn, Doss, Jennifer, Chi, Jen-Tsan PLoS Genet, 2013;9(4):e1003408. Species: Human Sample Type: Cell Lysates Application: WB Characterization of proprotein convertase subtilisin/kexin type 9 (PCSK9) trafficking reveals a novel lysosomal targeting mechanism via amyloid precursor-like protein 2 (APLP2). Authors: DeVay, Rachel M, Shelton, David L, Liang, Hong J Biol Chem, 2013;288(15):10805-18. Species: Human Sample Type: Cell Lysates Application: ELISA Development Proprotein convertase subtilisin/kexin type 7 (PCSK7) is essential for the zebrafish development and bioavailability of transforming growth factor beta1a (TGFbeta1a). Authors: Turpeinen H, Oksanen A, Kivinen V, Kukkurainen S, Uusimaki A, Ramet M, Parikka M, Hytonen V, Nykter M, Pesu M J Biol Chem, 2013;288(51):36610-23. Species: Human Sample Type: Application: WB Surface molecule CD229 as a novel target for the diagnosis and treatment of multiple myeloma. Authors: Atanackovic D, Panse J, Hildebrandt Y, Jadczak A, Kobold S, Cao Y, Templin J, Meyer S, Reinhard H, Bartels K, Lajmi N, Zander AR, Marx AH, Luetkens T, Bokemeyer C, Kroger N Haematologica, 2011;0(0):. Species: Human Sample Type: Cell Lysates Application: WB Anti-inflammatory activity of nanocrystalline silver-derived solutions in porcine contact dermatitis. Authors: Nadworny PL, Wang J, Tredget EE J Inflamm (Lond), 2010;7(0):13. Species: Porcine Sample Type: Whole Tissue Application: IHC Paraffin-embedded BAMBI is expressed in endothelial cells and is regulated by lysosomal/autolysosomal degradation. Authors: Xavier S, Gilbert V, Rastaldi MP, Krick S, Kollins D, Reddy A, Bottinger E, Cohen CD, Schlondorff D PLoS ONE, 2010;5(9):e12995. Species: Mouse Sample Type: Tissue Homogenates Application: WB Comparison of proteomic and transcriptomic profiles in the bronchial airway epithelium of current and never smokers. Authors: Steiling K, Kadar AY, Bergerat A PLoS ONE, 2009;4(4):e5043. Species: Human Sample Type: Cell Lysates Application: WB Mannan-binding lectin-associated serine protease 3 cleaves synthetic peptides and insulin-like growth factor-binding protein 5. Authors: Cortesio CL, Jiang W Arch. Biochem. Biophys., 2006;449(1):164-70. Species: N/A Sample Type: N/A Application: WB Characterization of cathepsin L secreted by Sf21 insect cells. Authors: Johnson GD, Jiang W Arch. Biochem. Biophys., 2005;444(1):7-14. Species: N/A Sample Type: N/A Application: WB Expand to show all Citations

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Mouse IgG HRP-conjugated Antibody

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Certificate of Analysis Request Form

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