Mouse IgG HRP-conjugated Antibody
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Summary
Data Examples
Preparation and Storage
Reconstitution Calculator
Background
Product Datasheets
Related Research Areas
Mouse IgG HRP-conjugated Antibody Summary
Species Reactivity
Mouse
Specificity
Detects mouse IgG1, IgG2A, IgG2B, and IgG3 heavy and light chains in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross‑reactivity with human IgG and rabbit IgG is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse IgG
Formulation
Sodium Phosphate and Proclin 300.
Label
HRP (Horseradish Peroxidase)
Applications
Recommended Concentration
Sample
Western Blot
Use at a 1:1000 dilution.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Western Blot
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Detection of Mouse IgG Primary Antibody byWestern Blot
Westernblot shows detection of Mouse Anti-Human/Mouse/Rat STAT3 Monoclonal Antibody
(Catalog #MAB1799)using a 1:1000 dilution of HRP-conjugated Anti-Mouse IgG Secondary Antibody
(Catalog # HAF007). This experiment wasconducted under reducing conditions and using ImmunoblotBuffer Group 1.
Reconstitution Calculator
Reconstitution Calculator
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Do not freeze.
- 6 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Long Name
Immunoglobulin G
Alternate Names
IgG; immunoglobulin heavy constant gamma 1 (G1m marker)
Product Datasheets
Citations for Mouse IgG HRP-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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AAV1.NT-3 gene therapy increases muscle fiber diameter through activation of mTOR pathway and metabolic remodeling in a CMT mouse model
Authors: ME Yalvac, J Amornvit, L Chen, KM Shontz, S Lewis, Z Sahenk
Gene Ther., 2018;0(0):.
Species: Mouse
Sample Types:
Applications: WB -
Key Proteins Involved in Spheroid Formation and Angiogenesis in Endothelial Cells After Long-Term Exposure to Simulated Microgravity
Authors: A Dittrich, D Grimm, J Sahana, J Bauer, M Krüger, M Infanger, NE Magnusson
Cell. Physiol. Biochem., 2018;45(2):429-445.
Species: Human
Sample Types: Cell Lysates
Applications: WB -
Allopregnanolone alters follicular and luteal dynamics during the estrous cycle
Authors: JA Asensio, ARR Cáceres, LT Pelegrina, MLÁ Sanhueza, L Scotti, F Parborell, MR Laconi
Reprod. Biol. Endocrinol., 2018;16(1):35.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
Low-dose paclitaxel downregulates MYC proto-oncogene bHLH transcription factor expression in colorectal carcinoma cells
Authors: W Li, W Zhu, C Lv, H Qu, K Xu, H Li, H Li, Y Du, G Liu, Y Wang, HJ Wei, HY Zhao
Oncol Lett, 2018;15(2):1881-1887.
Species: Human
Sample Types: Cell Lysates
Applications: WB -
Parthenolide reduces gene transcription of prosurvival mediators in U937 cells
Authors: S Mohammadi, M Zahedpanah, M Nikbakht, M Shaiegan, G Hamidollah, M Nikugoftar, B Rahmani, D Hamedi Asl
Exp. Oncol., 2017;39(1):30-35.
Species: Mouse
Sample Types:
Applications: WB -
Cellular Stress and p53-Associated Apoptosis by Juniperus communis L. Berry Extract Treatment in the Human SH-SY5Y Neuroblastoma Cells
Int J Mol Sci, 2016;17(7):.
Applications: WB -
Inflammatory Properties and Adjuvant Potential of Synthetic Glycolipids Homologous to Mycolate Esters of the Cell Wall of Mycobacterium tuberculosis
Authors: Marta Romano
J Innate Immun, 2016;0(0):.
Applications: Secondary antibody -
Gamma secretase inhibitor impairs epithelial-to-mesenchymal transition induced by TGF-? in ovarian tumor cell lines
Authors: M C Pazos
Mol. Cell. Endocrinol, 2016;440(0):125-137.
Species: Mouse
Sample Types: Antibody
Applications: WB -
Fasting selectively blocks development of acute lymphoblastic leukemia via leptin-receptor upregulation
Nat. Med, 2016;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: WB -
The GTPase ARF6 Controls ROS Production to Mediate Angiotensin II-Induced Vascular Smooth Muscle Cell Proliferation.
Authors: Bourmoum M, Charles R, Claing A
PLoS ONE, 2016;11(1):e0148097.
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microRNA-155, induced by interleukin-1ss, represses the expression of microphthalmia-associated transcription factor (MITF-M) in melanoma cells.
Authors: Arts N, Cane S, Hennequart M, Lamy J, Bommer G, Van den Eynde B, De Plaen E
PLoS ONE, 2015;10(4):e0122517.
Species: Human
Sample Types: Cell Lysates
Applications: Neut -
MicroRNA-7 Promotes Glycolysis to Protect against 1-Methyl-4-phenylpyridinium-induced Cell Death.
Authors: Chaudhuri A, Kabaria S, Choi D, Mouradian M, Junn E
J Biol Chem, 2015;290(19):12425-34.
Species: Human
Sample Types: Cell Lysates
Applications: WB -
Identification and characterization of PERK activators by phenotypic screening and their effects on NRF2 activation.
Authors: Xie W, Pariollaud M, Wixted W, Chitnis N, Fornwald J, Truong M, Pao C, Liu Y, Ames R, Callahan J, Solari R, Sanchez Y, Diehl A, Li H
PLoS ONE, 2015;10(3):e0119738.
Species: Mouse
Sample Types: Cell Lysates
Applications: WB -
Decidualization and syndecan-1 knock down sensitize endometrial stromal cells to apoptosis induced by embryonic stimuli.
Authors: Boeddeker S, Baston-Buest D, Fehm T, Kruessel J, Hess A
PLoS ONE, 2015;10(4):e0121103.
Species: Human
Sample Types: Cell Lysates
Applications: Bioassay -
Symmorphosis through dietary regulation: a combinatorial role for proteolysis, autophagy and protein synthesis in normalising muscle metabolism and function of hypertrophic mice after acute starvation.
Authors: Collins-Hooper H, Sartori R, Giallourou N, Matsakas A, Mitchell R, Makarenkova H, Flasskamp H, Macharia R, Ray S, Swann J, Sandri M, Patel K
PLoS ONE, 2015;10(3):e0120524.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: WB -
Mucosal pemphigus vulgaris anti-Dsg3 IgG is pathogenic to the oral mucosa of humanized Dsg3 mice.
Authors: Culton D, McCray S, Park M, Roberts J, Li N, Zedek D, Anhalt G, Cowley D, Liu Z, Diaz L
J Invest Dermatol, 2015;135(6):1590-7.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: WB -
Proangiogenic tumor proteins as potential predictive or prognostic biomarkers for bevacizumab therapy in metastatic colorectal cancer.
Authors: Bruhn M, Townsend A, Khoon Lee C, Shivasami A, Price T, Wrin J, Arentz G, Tebbutt N, Hocking C, Cunningham D, Hardingham J
Int J Cancer, 2014;135(3):731-41.
Species: Human
Sample Types: Tissue Homogenates
Applications: WB -
Cell proliferation and apoptosis in rat mammary glands following combinational exposure to bisphenol A and genistein.
Authors: Wang J, Jenkins S, Lamartiniere C
BMC Cancer, 2014;14(0):379.
Species: Rat
Sample Types: Cell Lysates
Applications: WB -
ADAMTS1 inhibits lymphangiogenesis by attenuating phosphorylation of the lymphatic endothelial cell-specific VEGF receptor.
Authors: Inagaki J, Takahashi K, Ogawa H, Asano K, Faruk Hatipoglu O, Cilek M, Obika M, Ohtsuki T, Hofmann M, Kusachi S, Ninomiya Y, Hirohata S
Exp Cell Res, 2014;323(2):263-75.
Species: Human
Sample Types: Cell Lysates
Applications: WB -
Exploring codon optimization and response surface methodology to express biologically active transmembrane RANKL in E. coli.
Authors: Maharjan S, Singh B, Bok J, Kim J, Jiang T, Cho C, Kang S, Choi Y
PLoS ONE, 2014;9(5):e96259.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: WB -
HIV and HCV activate the inflammasome in monocytes and macrophages via endosomal Toll-like receptors without induction of type 1 interferon.
Authors: Chattergoon M, Latanich R, Quinn J, Winter M, Buckheit R, Blankson J, Pardoll D, Cox A
PLoS Pathog, 2014;10(5):e1004082.
Species: Human
Sample Types: Cell Lysates
Applications: WB -
MicroRNA-7 protects against 1-methyl-4-phenylpyridinium-induced cell death by targeting RelA.
Authors: Choi D, Chae Y, Kabaria S, Chaudhuri A, Jain M, Li H, Mouradian M, Junn E
J Neurosci, 2014;34(38):12725-37.
Species: Human
Sample Types: Cell Lysates
Applications: WB -
Up-regulation of VEGF by retinoic acid during hyperoxia prevents retinal neovascularization and retinopathy.
Authors: Wang L, Shi P, Xu Z, Li J, Xie Y, Mitton K, Drenser K, Yan Q
Invest Ophthalmol Vis Sci, 2014;55(7):4276-87.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: WB -
Ligand-independent canonical Wnt activity in canine mammary tumor cell lines associated with aberrant LEF1 expression.
Authors: Gracanin A, Timmermans-Sprang E, van Wolferen M, Rao N, Grizelj J, Vince S, Hellmen E, Mol J
PLoS ONE, 2014;9(6):e98698.
Species: N/A
Sample Types: N/A
Applications: WB -
Proprotein convertase subtilisin/kexin type 7 (PCSK7) is essential for the zebrafish development and bioavailability of transforming growth factor beta1a (TGFbeta1a).
Authors: Turpeinen H, Oksanen A, Kivinen V, Kukkurainen S, Uusimaki A, Ramet M, Parikka M, Hytonen V, Nykter M, Pesu M
J Biol Chem, 2013;288(51):36610-23.
Species: N/A
Sample Types: N/A
Applications: WB -
Iron-responsive miR-485-3p regulates cellular iron homeostasis by targeting ferroportin.
Authors: Sangokoya, Carolyn, Doss, Jennifer, Chi, Jen-Tsan
PLoS Genet, 2013;9(4):e1003408.
Species: Human
Sample Types: Cell Lysates
Applications: WB -
Characterization of proprotein convertase subtilisin/kexin type 9 (PCSK9) trafficking reveals a novel lysosomal targeting mechanism via amyloid precursor-like protein 2 (APLP2).
Authors: DeVay, Rachel M, Shelton, David L, Liang, Hong
J Biol Chem, 2013;288(15):10805-18.
Species: Human
Sample Types: Cell Lysates
Applications: ELISA Development -
Surface molecule CD229 as a novel target for the diagnosis and treatment of multiple myeloma.
Authors: Atanackovic D, Panse J, Hildebrandt Y, Jadczak A, Kobold S, Cao Y, Templin J, Meyer S, Reinhard H, Bartels K, Lajmi N, Zander AR, Marx AH, Luetkens T, Bokemeyer C, Kroger N
Haematologica, 2011;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: WB -
Anti-inflammatory activity of nanocrystalline silver-derived solutions in porcine contact dermatitis.
Authors: Nadworny PL, Wang J, Tredget EE
J Inflamm (Lond), 2010;7(0):13.
Species: Porcine
Sample Types: Whole Tissue
Applications: IHC Paraffin-embedded -
BAMBI is expressed in endothelial cells and is regulated by lysosomal/autolysosomal degradation.
Authors: Xavier S, Gilbert V, Rastaldi MP, Krick S, Kollins D, Reddy A, Bottinger E, Cohen CD, Schlondorff D
PLoS ONE, 2010;5(9):e12995.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: WB -
Comparison of proteomic and transcriptomic profiles in the bronchial airway epithelium of current and never smokers.
Authors: Steiling K, Kadar AY, Bergerat A
PLoS ONE, 2009;4(4):e5043.
Species: Human
Sample Types: Cell Lysates
Applications: WB -
Mannan-binding lectin-associated serine protease 3 cleaves synthetic peptides and insulin-like growth factor-binding protein 5.
Authors: Cortesio CL, Jiang W
Arch. Biochem. Biophys., 2006;449(1):164-70.
Species: N/A
Sample Types: N/A
Applications: WB -
Characterization of cathepsin L secreted by Sf21 insect cells.
Authors: Johnson GD, Jiang W
Arch. Biochem. Biophys., 2005;444(1):7-14.
Species: N/A
Sample Types: N/A
Applications: WB
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Reviews for Mouse IgG HRP-conjugated Antibody
Average Rating: 4.6 (Based on 7 Reviews)
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Mouse IgG HRP-conjugated Antibody
By Anonymous on 05/01/2018
Application: ICC/IF
Sample Tested: Liver cancer tissue
Species: Mouse
Mouse IgG HRP-conjugated Antibody
By Anonymous on 04/16/2018
Application: ELISA
Sample Tested: Recombinant protein
Species: Human
We are using this product for Elisa assay for various human samples, we used at 1:1000 dilution, and it works very well, no artificial background.
Mouse IgG HRP-conjugated Antibody
By Anonymous on 02/22/2018
Application: WB
Sample Tested: T cells
Species: Human
Mouse anti-human beta Actin was used as primary and then R&D Systems goat-anti mouse was used a a secondary (Incubated for one hour at RT in 5% BSA). Membrane was developed using ECL substrate.
Mouse IgG HRP-conjugated Antibody
By Anonymous on 10/20/2017
Application: ELISA
Sample Tested: antibody
Species: Goat
Mouse IgG HRP-conjugated Antibody
By Anonymous on 06/28/2017
Application: WB
Sample Tested: Cell Lysates
Species: Mouse,Human
Mouse IgG Horseradish Peroxidase-conjugated Antibody
By Anonymous on 02/22/2016
Application: WB
Sample Tested: Adipose tissue,Small intestine
Species: Mouse
Mouse IgG Horseradish Peroxidase-conjugated Antibody
By Anonymous on 02/22/2016
Application: WB
Sample Tested: Cell Lysates
Species: E. coli
