Mouse IgG HRP-conjugated Antibody Summary
Applications
Use at a 1:1000 dilution.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example

Detection of Mouse IgG Primary Antibody by Western Blot Western blot shows detection of Mouse Anti-Human/Mouse/Rat STAT3 Monoclonal Antibody (Catalog # MAB1799) using a 1:1000 dilution of HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Preparation and Storage
- 6 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Product Datasheets
Citations for Mouse IgG HRP-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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RAGE impairs murine diabetic atherosclerosis regression and implicates IRF7 in macrophage inflammation and cholesterol metabolism
Authors: L Senatus, R López-Díez, L Egaña-Gorr, J Liu, J Hu, G Daffu, Q Li, K Rahman, Y Vengrenyuk, TJ Barrett, MZ Dewan, L Guo, D Fuller, AV Finn, R Virmani, H Li, RA Friedman, EA Fisher, R Ramasamy, AM Schmidt
JCI Insight, 2020;5(13):.
Species: Mouse
Sample Types: Plasma
Applications: Western Blot -
Chemotherapeutic Agents Sensitize Resistant Cancer Cells to the DR5-Specific Variant DR5-B more Efficiently than to TRAIL by Modulating the Surface Expression of Death and Decoy Receptors
Authors: ?? ?rtykov, DA Belov, VO Shipunova, DB Trushina, SM Deyev, DA Dolgikh, MP Kirpichnik, ME Gasparian
Cancers (Basel), 2020;12(5):.
Species: Human
Sample Types: Whole Cells
Applications: Western Blot -
Epigallocatechin-3-gallate (EGCG) Alters Histone Acetylation and Methylation and Impacts Chromatin Architecture Profile in Human Endothelial Cells
Authors: O Ciesielski, M Biesiekier, A Balcerczyk
Molecules, 2020;25(10):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Immunogenicity and Protective Effect of a Virus-Like Particle Containing the SAG1 Antigen of Toxoplasma gondii as a Potential Vaccine Candidate for Toxoplasmosis
Authors: WH Choi, JS Park
Biomedicines, 2020;8(4):.
Species: Mouse
Sample Types: blood
Applications: Functional Assay -
Tissue factor expression predicts outcome in children with neuroblastoma: A retrospective study
Authors: LM Sherief, TH Hassan, M Zakaria, M Fathy, EA Eshak, MA Bebars, A Esh
Oncol Lett, 2019;18(6):6347-6354.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Purification and characterization of a fimbrial protein from Porphyromonas salivosa ATCC 49407
Authors: Y Koyata, K Watanabe, T Toyama, H Sasaki, N Hamada
J. Vet. Med. Sci., 2019;0(0):.
Species: Mouse
Sample Types: Protein
Applications: Western Blot -
P-cadherin mutations are associated with high basal Wnt activity and stemness in canine mammary tumor cell lines
Authors: E Timmermans, R Collin, A Henkes, M Philipsen, JA Mol
Oncotarget, 2019;10(31):2930-2946.
Species: Canine
Sample Types: Cell Lysates
Applications: Western Blot -
The Structural Function of Nestin in Cell Body Softening is Correlated with Cancer Cell Metastasis
Authors: A Yamagishi, M Susaki, Y Takano, M Mizusawa, M Mishima, M Iijima, S Kuroda, T Okada, C Nakamura
Int. J. Biol. Sci., 2019;15(7):1546-1556.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Induction of 3-hydroxy-3-methylglutaryl-CoA reductase mediates statin resistance in breast cancer cells
Authors: A Göbel, D Breining, M Rauner, LC Hofbauer, TD Rachner
Cell Death Dis, 2019;10(2):91.
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UBE2T promotes proliferation and regulates PI3K/Akt signaling in renal cell carcinoma
Authors: P Hao, B Kang, Y Li, W Hao, F Ma
Mol Med Rep, 2019;20(2):1212-1220.
Species: Human
Sample Types: Protein
Applications: Western Blot -
Lipid storage droplet protein 5 reduces sodium palmitate?induced lipotoxicity in human normal liver cells by regulating lipid metabolism?related factors
Authors: X Ma, F Cheng, K Yuan, K Jiang, T Zhu
Mol Med Rep, 2019;20(2):879-886.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Silencing Lysine-Specific Histone Demethylase 1 (LSD1) Causes Increased HP1-Positive Chromatin, Stimulation of DNA Repair Processes, and Dysregulation of Proliferation by Chk1 Phosphorylation in Human Endothelial Cells
Authors: M Wojtala, A D?bek, D Rybaczek, A ?liwi?ska, E ?widerska, K S?apek, A El-Osta, A Balcerczyk
Cells, 2019;8(10):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Thioredoxin Reductase-1 Inhibition Augments Endogenous Glutathione-Dependent Antioxidant Responses in Experimental Bronchopulmonary Dysplasia
Authors: SB Wall, R Wood, K Dunigan, Q Li, R Li, LK Rogers, TE Tipple
Oxid Med Cell Longev, 2019;2019(0):7945983.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Proteomics analysis of colon cancer progression
Authors: S Saleem, S Tariq, I Aleem, Sadr-Ul Sh, M Tahseen, A Atiq, S Hassan, M Abu Bakar, S Khattak, AA Syed, AH Ahmad, M Hussain, MA Yusuf, C Sutton
Clin Proteomics, 2019;16(0):44.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot -
Effects of age on feeding response: Focus on the rostral C1 neuron and its glucoregulatory proteins
Authors: H Ramlan, HA Damanhuri
Exp. Gerontol., 2019;129(0):110779.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot -
Bee venom phospholipase A2 ameliorates Alzheimer's disease pathology in A? vaccination treatment without inducing neuro-inflammation in a 3xTg-AD mouse model
Authors: H Baek, CJ Lee, DB Choi, NS Kim, YS Kim, YJ Ye, YS Kim, JS Kim, I Shim, H Bae
Sci Rep, 2018;8(1):17369.
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Influence of bradykinin B2 receptor and dopamine D2 receptor on the oxidative stress, inflammatory response, and apoptotic process in human endothelial cells
Authors: A Niewiarows, A Kozik, I Guevara-Lo
PLoS ONE, 2018;13(11):e0206443.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Microphthalmia-Associated Transcription Factor (MITF) Regulates Immune Cell Migration into Melanoma
Authors: GM Wiedemann, C Aithal, A Kraechan, C Heise, BL Cadilha, J Zhang, P Duewell, R Ballotti, S Endres, C Bertolotto, S Kobold
Transl Oncol, 2018;12(2):350-360.
Species: Mouse
Sample Types: Cell Lysates
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The Parkinson's disease gene product DJ-1 modulates miR-221 to promote neuronal survival against oxidative stress
Authors: SE Oh, HJ Park, L He, C Skibiel, E Junn, MM Mouradian
Redox Biol, 2018;19(0):62-73.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
mRNA vaccination with charge-altering releasable transporters elicits human T cell responses and cures established tumors in mice
Authors: OAW Haabeth, TR Blake, CJ McKinlay, RM Waymouth, PA Wender, R Levy
Proc. Natl. Acad. Sci. U.S.A., 2018;0(0):.
Species: Mouse
Sample Types: Plasma
Applications: ELISA Development (Secondary) -
Liraglutide Treatment Reduces Endothelial Endoplasmic Reticulum Stress and Insulin Resistance in Patients With Diabetes Mellitus
Authors: R Bretón-Rom, RM Weisbrod, B Feng, M Holbrook, D Ko, MM Stathos, JY Zhang, JL Fetterman, NM Hamburg
J Am Heart Assoc, 2018;7(18):e009379.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Key Proteins Involved in Spheroid Formation and Angiogenesis in Endothelial Cells After Long-Term Exposure to Simulated Microgravity
Authors: A Dittrich, D Grimm, J Sahana, J Bauer, M Krüger, M Infanger, NE Magnusson
Cell. Physiol. Biochem., 2018;45(2):429-445.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Allopregnanolone alters follicular and luteal dynamics during the estrous cycle
Authors: JA Asensio, ARR Cáceres, LT Pelegrina, MLÁ Sanhueza, L Scotti, F Parborell, MR Laconi
Reprod. Biol. Endocrinol., 2018;16(1):35.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
Bat lung epithelial cells show greater host species-specific innate resistance than MDCK cells to human and avian influenza viruses
Authors: T Slater, I Eckerle, KC Chang
Virol. J., 2018;15(1):68.
Species: Bat
Sample Types: Cell Lysates
Applications: Western Blot -
Low-dose paclitaxel downregulates MYC proto-oncogene bHLH transcription factor expression in colorectal carcinoma cells
Authors: W Li, W Zhu, C Lv, H Qu, K Xu, H Li, H Li, Y Du, G Liu, Y Wang, HJ Wei, HY Zhao
Oncol Lett, 2018;15(2):1881-1887.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
The Role of Autophagy in the Degradation of Misfolded HLA-B27 Heavy Chains
Authors: F Navid, G Layh-Schmi, KA Sikora, A Cougnoux, RA Colbert
Arthritis & rheumatology (Hoboken, N.J.), 2018;0(0):.
Species: Rat
Sample Types: Whole Cells
Applications: Western Blot -
Deregulation of microRNA?31a?5p is involved in the development of primary hypertension by suppressing apoptosis of pulmonary artery smooth muscle cells via targeting TP53
Authors: Q Feng, T Tian, J Liu, L Zhang, J Qi, X Lin
Int. J. Mol. Med., 2018;0(0):.
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot -
AAV1.NT-3 gene therapy increases muscle fiber diameter through activation of mTOR pathway and metabolic remodeling in a CMT mouse model
Authors: ME Yalvac, J Amornvit, L Chen, KM Shontz, S Lewis, Z Sahenk
Gene Ther., 2018;0(0):.
Species: Mouse
Sample Types:
Applications: Western Blot -
Parthenolide reduces gene transcription of prosurvival mediators in U937 cells
Authors: S Mohammadi, M Zahedpanah, M Nikbakht, M Shaiegan, G Hamidollah, M Nikugoftar, B Rahmani, D Hamedi Asl
Exp. Oncol., 2017;39(1):30-35.
Species: Mouse
Sample Types:
Applications: Western Blot -
Gamma secretase inhibitor impairs epithelial-to-mesenchymal transition induced by TGF-? in ovarian tumor cell lines
Authors: M C Pazos
Mol. Cell. Endocrinol, 2016;440(0):125-137.
Species: Mouse
Sample Types: Antibody
Applications: Western Blot -
Inflammatory Properties and Adjuvant Potential of Synthetic Glycolipids Homologous to Mycolate Esters of the Cell Wall of Mycobacterium tuberculosis
Authors: Marta Romano
J Innate Immun, 2016;0(0):.
Applications: Secondary Antibody -
Cellular Stress and p53-Associated Apoptosis by Juniperus communis L. Berry Extract Treatment in the Human SH-SY5Y Neuroblastoma Cells
Int J Mol Sci, 2016;17(7):.
Applications: Western Blot -
The GTPase ARF6 Controls ROS Production to Mediate Angiotensin II-Induced Vascular Smooth Muscle Cell Proliferation.
Authors: Bourmoum M, Charles R, Claing A
PLoS ONE, 2016;11(1):e0148097.
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Fasting selectively blocks development of acute lymphoblastic leukemia via leptin-receptor upregulation
Nat. Med, 2016;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Decidualization and syndecan-1 knock down sensitize endometrial stromal cells to apoptosis induced by embryonic stimuli.
Authors: Boeddeker S, Baston-Buest D, Fehm T, Kruessel J, Hess A
PLoS ONE, 2015;10(4):e0121103.
Species: Human
Sample Types: Cell Lysates
Applications: Bioassay -
Symmorphosis through dietary regulation: a combinatorial role for proteolysis, autophagy and protein synthesis in normalising muscle metabolism and function of hypertrophic mice after acute starvation.
Authors: Collins-Hooper H, Sartori R, Giallourou N, Matsakas A, Mitchell R, Makarenkova H, Flasskamp H, Macharia R, Ray S, Swann J, Sandri M, Patel K
PLoS ONE, 2015;10(3):e0120524.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Identification and characterization of PERK activators by phenotypic screening and their effects on NRF2 activation.
Authors: Xie W, Pariollaud M, Wixted W, Chitnis N, Fornwald J, Truong M, Pao C, Liu Y, Ames R, Callahan J, Solari R, Sanchez Y, Diehl A, Li H
PLoS ONE, 2015;10(3):e0119738.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
microRNA-155, induced by interleukin-1ss, represses the expression of microphthalmia-associated transcription factor (MITF-M) in melanoma cells.
Authors: Arts N, Cane S, Hennequart M, Lamy J, Bommer G, Van den Eynde B, De Plaen E
PLoS ONE, 2015;10(4):e0122517.
Species: Human
Sample Types: Cell Lysates
Applications: Neutralization -
MicroRNA-7 Promotes Glycolysis to Protect against 1-Methyl-4-phenylpyridinium-induced Cell Death.
Authors: Chaudhuri A, Kabaria S, Choi D, Mouradian M, Junn E
J Biol Chem, 2015;290(19):12425-34.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Mucosal pemphigus vulgaris anti-Dsg3 IgG is pathogenic to the oral mucosa of humanized Dsg3 mice.
Authors: Culton D, McCray S, Park M, Roberts J, Li N, Zedek D, Anhalt G, Cowley D, Liu Z, Diaz L
J Invest Dermatol, 2015;135(6):1590-7.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Proangiogenic tumor proteins as potential predictive or prognostic biomarkers for bevacizumab therapy in metastatic colorectal cancer.
Authors: Bruhn M, Townsend A, Khoon Lee C, Shivasami A, Price T, Wrin J, Arentz G, Tebbutt N, Hocking C, Cunningham D, Hardingham J
Int J Cancer, 2014;135(3):731-41.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot -
ADAMTS1 inhibits lymphangiogenesis by attenuating phosphorylation of the lymphatic endothelial cell-specific VEGF receptor.
Authors: Inagaki J, Takahashi K, Ogawa H, Asano K, Faruk Hatipoglu O, Cilek M, Obika M, Ohtsuki T, Hofmann M, Kusachi S, Ninomiya Y, Hirohata S
Exp Cell Res, 2014;323(2):263-75.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Cell proliferation and apoptosis in rat mammary glands following combinational exposure to bisphenol A and genistein.
Authors: Wang J, Jenkins S, Lamartiniere C
BMC Cancer, 2014;14(0):379.
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot -
HIV and HCV activate the inflammasome in monocytes and macrophages via endosomal Toll-like receptors without induction of type 1 interferon.
Authors: Chattergoon M, Latanich R, Quinn J, Winter M, Buckheit R, Blankson J, Pardoll D, Cox A
PLoS Pathog, 2014;10(5):e1004082.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Exploring codon optimization and response surface methodology to express biologically active transmembrane RANKL in E. coli.
Authors: Maharjan S, Singh B, Bok J, Kim J, Jiang T, Cho C, Kang S, Choi Y
PLoS ONE, 2014;9(5):e96259.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: Western Blot -
MicroRNA-7 protects against 1-methyl-4-phenylpyridinium-induced cell death by targeting RelA.
Authors: Choi D, Chae Y, Kabaria S, Chaudhuri A, Jain M, Li H, Mouradian M, Junn E
J Neurosci, 2014;34(38):12725-37.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Up-regulation of VEGF by retinoic acid during hyperoxia prevents retinal neovascularization and retinopathy.
Authors: Wang L, Shi P, Xu Z, Li J, Xie Y, Mitton K, Drenser K, Yan Q
Invest Ophthalmol Vis Sci, 2014;55(7):4276-87.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Ligand-independent canonical Wnt activity in canine mammary tumor cell lines associated with aberrant LEF1 expression.
Authors: Gracanin A, Timmermans-Sprang E, van Wolferen M, Rao N, Grizelj J, Vince S, Hellmen E, Mol J
PLoS ONE, 2014;9(6):e98698.
Species: N/A
Sample Types: N/A
Applications: Western Blot -
Iron-responsive miR-485-3p regulates cellular iron homeostasis by targeting ferroportin.
Authors: Sangokoya, Carolyn, Doss, Jennifer, Chi, Jen-Tsan
PLoS Genet, 2013;9(4):e1003408.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Proprotein convertase subtilisin/kexin type 7 (PCSK7) is essential for the zebrafish development and bioavailability of transforming growth factor beta1a (TGFbeta1a).
Authors: Turpeinen H, Oksanen A, Kivinen V, Kukkurainen S, Uusimaki A, Ramet M, Parikka M, Hytonen V, Nykter M, Pesu M
J Biol Chem, 2013;288(51):36610-23.
Species: N/A
Sample Types: N/A
Applications: Western Blot -
Characterization of proprotein convertase subtilisin/kexin type 9 (PCSK9) trafficking reveals a novel lysosomal targeting mechanism via amyloid precursor-like protein 2 (APLP2).
Authors: DeVay, Rachel M, Shelton, David L, Liang, Hong
J Biol Chem, 2013;288(15):10805-18.
Species: Human
Sample Types: Cell Lysates
Applications: ELISA Development -
Surface molecule CD229 as a novel target for the diagnosis and treatment of multiple myeloma.
Authors: Atanackovic D, Panse J, Hildebrandt Y, Jadczak A, Kobold S, Cao Y, Templin J, Meyer S, Reinhard H, Bartels K, Lajmi N, Zander AR, Marx AH, Luetkens T, Bokemeyer C, Kroger N
Haematologica, 2011;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Anti-inflammatory activity of nanocrystalline silver-derived solutions in porcine contact dermatitis.
Authors: Nadworny PL, Wang J, Tredget EE
J Inflamm (Lond), 2010;7(0):13.
Species: Porcine
Sample Types: Whole Tissue
Applications: IHC-P -
BAMBI is expressed in endothelial cells and is regulated by lysosomal/autolysosomal degradation.
Authors: Xavier S, Gilbert V, Rastaldi MP, Krick S, Kollins D, Reddy A, Bottinger E, Cohen CD, Schlondorff D
PLoS ONE, 2010;5(9):e12995.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Comparison of proteomic and transcriptomic profiles in the bronchial airway epithelium of current and never smokers.
Authors: Steiling K, Kadar AY, Bergerat A
PLoS ONE, 2009;4(4):e5043.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Mannan-binding lectin-associated serine protease 3 cleaves synthetic peptides and insulin-like growth factor-binding protein 5.
Authors: Cortesio CL, Jiang W
Arch. Biochem. Biophys., 2006;449(1):164-70.
Species: N/A
Sample Types: N/A
Applications: Western Blot -
Characterization of cathepsin L secreted by Sf21 insect cells.
Authors: Johnson GD, Jiang W
Arch. Biochem. Biophys., 2005;444(1):7-14.
Species: N/A
Sample Types: N/A
Applications: Western Blot
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used as secondary antibody for WB at 1:1000 with 1hr incubation at RT
We are using this product for Elisa assay for various human samples, we used at 1:1000 dilution, and it works very well, no artificial background.
Mouse anti-human beta Actin was used as primary and then R&D Systems goat-anti mouse was used a a secondary (Incubated for one hour at RT in 5% BSA). Membrane was developed using ECL substrate.