Mouse IgG HRP-conjugated Antibody

  (29 citations)
(6 Reviews)
    
Datasheet / CoA / SDS
Product Details
Citations (29)
FAQs
Supplemental Products
Reviews
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse IgG1, IgG2A, IgG2B, and IgG3 heavy and light chains in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross‑reactivity with human IgG and rabbit IgG is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse IgG
  • Formulation
    Sodium Phosphate and Proclin 300.
  • Label
    HRP (Horseradish Peroxidase)
Product Datasheets

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Certificate of Analysis Lookup

Certificate of Analysis Request Form

To download a Certificate of Analysis, please enter the catalog and lot numbers in the search box below.

Note: Certificate of Analysis not available for kit components.

Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot

    Use at a 1:1000 dilution.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of Mouse IgG Primary Antibody byWestern Blot Westernblot shows detection of Mouse Anti-Human/Mouse/Rat STAT3 Monoclonal Antibody
(Catalog #MAB1799)using a 1:1000 dilution of HRP-conjugated Anti-Mouse IgG Secondary Antibody
(Catalog # HAF007).  This experiment wasconducted under reducing conditions and using ImmunoblotBuffer Group 1.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Do not freeze.
    • 6 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

  • Long Name:
    Immunoglobulin G
  • Alternate Names:
    IgG; immunoglobulin heavy constant gamma 1 (G1m marker)
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

29 Citations: Showing 1 - 10
Filter your results:

Species
Applications
Sample Type
  1. AAV1.NT-3 gene therapy increases muscle fiber diameter through activation of mTOR pathway and metabolic remodeling in a CMT mouse model
    Authors: ME Yalvac, J Amornvit, L Chen, KM Shontz, S Lewis, Z Sahenk
    Gene Ther., 2018;0(0):.
    Species: Mouse
    Sample Type:
    Application: WB
  2. Key Proteins Involved in Spheroid Formation and Angiogenesis in Endothelial Cells After Long-Term Exposure to Simulated Microgravity
    Authors: A Dittrich, D Grimm, J Sahana, J Bauer, M Krüger, M Infanger, NE Magnusson
    Cell. Physiol. Biochem., 2018;45(2):429-445.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  3. Low-dose paclitaxel downregulates MYC proto-oncogene bHLH transcription factor expression in colorectal carcinoma cells
    Authors: W Li, W Zhu, C Lv, H Qu, K Xu, H Li, H Li, Y Du, G Liu, Y Wang, HJ Wei, HY Zhao
    Oncol Lett, 2018;15(2):1881-1887.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  4. Cellular Stress and p53-Associated Apoptosis by Juniperus communis L. Berry Extract Treatment in the Human SH-SY5Y Neuroblastoma Cells
    Int J Mol Sci, 2016;17(7):.
    Application: WB
  5. Fasting selectively blocks development of acute lymphoblastic leukemia via leptin-receptor upregulation
    Nat. Med, 2016;0(0):.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  6. The GTPase ARF6 Controls ROS Production to Mediate Angiotensin II-Induced Vascular Smooth Muscle Cell Proliferation.
    Authors: Bourmoum M, Charles R, Claing A
    PLoS ONE, 2016;11(1):e0148097.
  7. Mucosal pemphigus vulgaris anti-Dsg3 IgG is pathogenic to the oral mucosa of humanized Dsg3 mice.
    Authors: Culton D, McCray S, Park M, Roberts J, Li N, Zedek D, Anhalt G, Cowley D, Liu Z, Diaz L
    J Invest Dermatol, 2015;135(6):1590-7.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  8. Decidualization and syndecan-1 knock down sensitize endometrial stromal cells to apoptosis induced by embryonic stimuli.
    Authors: Boeddeker S, Baston-Buest D, Fehm T, Kruessel J, Hess A
    PLoS ONE, 2015;10(4):e0121103.
    Species: Human
    Sample Type: Cell Lysates
    Application: Bioassay
  9. MicroRNA-7 Promotes Glycolysis to Protect against 1-Methyl-4-phenylpyridinium-induced Cell Death.
    Authors: Chaudhuri A, Kabaria S, Choi D, Mouradian M, Junn E
    J Biol Chem, 2015;290(19):12425-34.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  10. Identification and characterization of PERK activators by phenotypic screening and their effects on NRF2 activation.
    Authors: Xie W, Pariollaud M, Wixted W, Chitnis N, Fornwald J, Truong M, Pao C, Liu Y, Ames R, Callahan J, Solari R, Sanchez Y, Diehl A, Li H
    PLoS ONE, 2015;10(3):e0119738.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  11. microRNA-155, induced by interleukin-1ss, represses the expression of microphthalmia-associated transcription factor (MITF-M) in melanoma cells.
    Authors: Arts N, Cane S, Hennequart M, Lamy J, Bommer G, Van den Eynde B, De Plaen E
    PLoS ONE, 2015;10(4):e0122517.
    Species: Human
    Sample Type: Cell Lysates
    Application: Neut
  12. Symmorphosis through dietary regulation: a combinatorial role for proteolysis, autophagy and protein synthesis in normalising muscle metabolism and function of hypertrophic mice after acute starvation.
    Authors: Collins-Hooper H, Sartori R, Giallourou N, Matsakas A, Mitchell R, Makarenkova H, Flasskamp H, Macharia R, Ray S, Swann J, Sandri M, Patel K
    PLoS ONE, 2015;10(3):e0120524.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  13. HIV and HCV activate the inflammasome in monocytes and macrophages via endosomal Toll-like receptors without induction of type 1 interferon.
    Authors: Chattergoon M, Latanich R, Quinn J, Winter M, Buckheit R, Blankson J, Pardoll D, Cox A
    PLoS Pathog, 2014;10(5):e1004082.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  14. Exploring codon optimization and response surface methodology to express biologically active transmembrane RANKL in E. coli.
    Authors: Maharjan S, Singh B, Bok J, Kim J, Jiang T, Cho C, Kang S, Choi Y
    PLoS ONE, 2014;9(5):e96259.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: WB
  15. Ligand-independent canonical Wnt activity in canine mammary tumor cell lines associated with aberrant LEF1 expression.
    Authors: Gracanin A, Timmermans-Sprang E, van Wolferen M, Rao N, Grizelj J, Vince S, Hellmen E, Mol J
    PLoS ONE, 2014;9(6):e98698.
    Species: N/A
    Sample Type: N/A
    Application: WB
  16. MicroRNA-7 protects against 1-methyl-4-phenylpyridinium-induced cell death by targeting RelA.
    Authors: Choi D, Chae Y, Kabaria S, Chaudhuri A, Jain M, Li H, Mouradian M, Junn E
    J Neurosci, 2014;34(38):12725-37.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  17. ADAMTS1 inhibits lymphangiogenesis by attenuating phosphorylation of the lymphatic endothelial cell-specific VEGF receptor.
    Authors: Inagaki J, Takahashi K, Ogawa H, Asano K, Faruk Hatipoglu O, Cilek M, Obika M, Ohtsuki T, Hofmann M, Kusachi S, Ninomiya Y, Hirohata S
    Exp Cell Res, 2014;323(2):263-75.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  18. Proangiogenic tumor proteins as potential predictive or prognostic biomarkers for bevacizumab therapy in metastatic colorectal cancer.
    Authors: Bruhn M, Townsend A, Khoon Lee C, Shivasami A, Price T, Wrin J, Arentz G, Tebbutt N, Hocking C, Cunningham D, Hardingham J
    Int J Cancer, 2014;135(3):731-41.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: WB
  19. Cell proliferation and apoptosis in rat mammary glands following combinational exposure to bisphenol A and genistein.
    Authors: Wang J, Jenkins S, Lamartiniere C
    BMC Cancer, 2014;14(0):379.
    Species: Rat
    Sample Type: Cell Lysates
    Application: WB
  20. Up-regulation of VEGF by retinoic acid during hyperoxia prevents retinal neovascularization and retinopathy.
    Authors: Wang L, Shi P, Xu Z, Li J, Xie Y, Mitton K, Drenser K, Yan Q
    Invest Ophthalmol Vis Sci, 2014;55(7):4276-87.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  21. Iron-responsive miR-485-3p regulates cellular iron homeostasis by targeting ferroportin.
    Authors: Sangokoya, Carolyn, Doss, Jennifer, Chi, Jen-Tsan
    PLoS Genet, 2013;9(4):e1003408.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  22. Characterization of proprotein convertase subtilisin/kexin type 9 (PCSK9) trafficking reveals a novel lysosomal targeting mechanism via amyloid precursor-like protein 2 (APLP2).
    Authors: DeVay, Rachel M, Shelton, David L, Liang, Hong
    J Biol Chem, 2013;288(15):10805-18.
    Species: Human
    Sample Type: Cell Lysates
    Application: ELISA Development
  23. Proprotein convertase subtilisin/kexin type 7 (PCSK7) is essential for the zebrafish development and bioavailability of transforming growth factor beta1a (TGFbeta1a).
    Authors: Turpeinen H, Oksanen A, Kivinen V, Kukkurainen S, Uusimaki A, Ramet M, Parikka M, Hytonen V, Nykter M, Pesu M
    J Biol Chem, 2013;288(51):36610-23.
    Species: Human
    Sample Type:
    Application: WB
  24. Surface molecule CD229 as a novel target for the diagnosis and treatment of multiple myeloma.
    Authors: Atanackovic D, Panse J, Hildebrandt Y, Jadczak A, Kobold S, Cao Y, Templin J, Meyer S, Reinhard H, Bartels K, Lajmi N, Zander AR, Marx AH, Luetkens T, Bokemeyer C, Kroger N
    Haematologica, 2011;0(0):.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  25. Anti-inflammatory activity of nanocrystalline silver-derived solutions in porcine contact dermatitis.
    Authors: Nadworny PL, Wang J, Tredget EE
    J Inflamm (Lond), 2010;7(0):13.
    Species: Porcine
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  26. BAMBI is expressed in endothelial cells and is regulated by lysosomal/autolysosomal degradation.
    Authors: Xavier S, Gilbert V, Rastaldi MP, Krick S, Kollins D, Reddy A, Bottinger E, Cohen CD, Schlondorff D
    PLoS ONE, 2010;5(9):e12995.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  27. Comparison of proteomic and transcriptomic profiles in the bronchial airway epithelium of current and never smokers.
    Authors: Steiling K, Kadar AY, Bergerat A
    PLoS ONE, 2009;4(4):e5043.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  28. Mannan-binding lectin-associated serine protease 3 cleaves synthetic peptides and insulin-like growth factor-binding protein 5.
    Authors: Cortesio CL, Jiang W
    Arch. Biochem. Biophys., 2006;449(1):164-70.
    Species: N/A
    Sample Type: N/A
    Application: WB
  29. Characterization of cathepsin L secreted by Sf21 insect cells.
    Authors: Johnson GD, Jiang W
    Arch. Biochem. Biophys., 2005;444(1):7-14.
    Species: N/A
    Sample Type: N/A
    Application: WB
Expand to show all Citations

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Secondary Antibodies
Description Application Cat# Citations Images  

Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 12  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow , IHC , ICC NL007 10
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow , IHC , ICC NL008 8
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 3  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG Antibody

WB AF007 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow , IHC , ICC NL009 5
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Average Rating: 4.5 (Based on 6 reviews)

Have you used Mouse IgG HRP-conjugated Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Read what people are saying who have used Mouse IgG HRP-conjugated Antibody.

We have 6 reviews tested in 5 species: E. coli, Goat, Human, Mouse, Mouse,Human.
We have 6 reviews tested in 3 applications: We are using this product for Elisa assay for various human samples, we used at 1:1000 dilution, and it works very well, no artificial background. , Western Blot, ELISA.

Filter by Ratings
Excellent
(4)
Very Good
(1)
Average
(1)
All Ratings
Filter by Applications
We are using this product for Elisa assay for various human samples, we used at 1:1000 dilution, and it works very well, no artificial background.
(1)
WB
(4)
ELISA
(1)
All Applications
Filter by Species
E. coli
(1)
Goat
(1)
Human
(2)
Mouse
(1)
Mouse,Human
(1)
All Species

Images Ratings Applications Species Reviewed By Date Details
  Excellent
 We are using this product for Elisa assay for various human samples, we used at 1:1000 dilution, and it works very well, no artificial background. Human Anonymous 04/16/2018
Show

Summary

Application We are using this product for Elisa assay for various human samples, we used at 1:1000 dilution, and it works very well, no artificial background.
Sample Tested Recombinant protein
Species Human
Western Blot Mouse IgG HRP-conjugated Antibody HAF007 Array HAF007
Enlarge
Excellent
 WB Human Anonymous 02/22/2018
Show
Image Details
Western Blot Mouse IgG HRP-conjugated Antibody HAF007
Western Blot: Mouse IgG HRP-conjugated Antibody [HAF007].

Summary

Application Western Blot
Sample Tested T cells
Species Human

Other Experimental Details

Other Experimental Details Mouse anti-human beta Actin was used as primary and then R&D Systems goat-anti mouse was used a a secondary (Incubated for one hour at RT in 5% BSA). Membrane was developed using ECL substrate.
  Excellent
 ELISA Goat Anonymous 10/20/2017
Show

Summary

Application ELISA
Sample Tested antibody
Species Goat
Western Blot Mouse IgG HRP-conjugated Antibody HAF007 Array HAF007
Enlarge
Excellent
 WB Mouse,Human Anonymous 06/28/2017
Show
Image Details
Western Blot Mouse IgG HRP-conjugated Antibody HAF007
Western Blot: Mouse IgG HRP-conjugated Antibody [HAF007].

Summary

Application Western Blot
Sample Tested Cell Lysates
Species Mouse,Human
Western Blot Mouse IgG Horseradish Peroxidase-conjugated Antibody HAF007 Array HAF007
Enlarge
Very Good
 WB Mouse Anonymous 02/22/2016
Show
Image Details
Western Blot Mouse IgG Horseradish Peroxidase-conjugated Antibody HAF007
Western Blot: Mouse IgG Horseradish Peroxidase-conjugated Antibody [HAF007].

Summary

Application Western Blot
Sample Tested Adipose tissue,Small intestine
Species Mouse
Western Blot Mouse IgG Horseradish Peroxidase-conjugated Antibody HAF007 Array HAF007
Enlarge
Average
 WB E. coli Anonymous 02/22/2016
Show
Image Details
Western Blot Mouse IgG Horseradish Peroxidase-conjugated Antibody HAF007
Western Blot: Mouse IgG Horseradish Peroxidase-conjugated Antibody [HAF007].

Summary

Application Western Blot
Sample Tested Cell Lysates
Species E. coli

Please scroll the above reviews for more

Order Details

Contact Us to Order
reload

Feedback

Customer Information
Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies
R&D Systems Guarantee