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Mouse IgG Horseradish Peroxidase-conjugated Antibody

R&D Systems | Catalog # HAF007

R&D Systems

Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Western Blot

Label

HRP

Antibody Source

Polyclonal Goat IgG
View all IgG Secondary Antibodies »

Product Specifications

Immunogen

Mouse IgG

Specificity

Detects mouse IgG

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse IgG Horseradish Peroxidase-conjugated Antibody

Detection of Mouse IgG Primary Antibody by Western Blot

Detection of Mouse IgG Primary Antibody by Western Blot

Western blot shows detection of Mouse Anti-Human/Mouse/Rat STAT3 Monoclonal Antibody (MAB1799) using a 1:1000 dilution of HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Myocilin in human skeletal muscle

Detection of Myocilin in human skeletal muscle using 5 ug/mL of Mouse monoclonal anti-human Myocilin primary antibody (MAB3446) followed by 30 min incubation with Goat anti Mouse HRP conjugated secondary antibodies (Catalog # HAF007) at 1:20 dilution + DAB chromogen (brown). The tissue was counterstained with Hematoxylin (blue). Control was done by omitting primary antibody.
Detection of Human IgG by Western Blot

Detection of Human IgG by Western Blot

Inverse correlation between the expression of MITF-M and that of miR-155 in melanoma cell lines incubated with IL-1 beta.(A) The expression of MITF-M, Tyrosinase and miR-155 was analyzed by quantitative RT-PCR in 8 melanoma cell lines incubated with IL-1 beta (10 ng/ml) for 4h or 24h. The results were normalized to beta -actin expression for MITF-M and Tyrosinase and to RNU44 for miR-155 (means ± SD for 3 independent experiments). (B) The expression of MITF-M and Tyrosinase proteins was analyzed by Western Blot in the same samples (1 of 3 independent experiments). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25853464), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human IgG by Western Blot

Detection of Human IgG by Western Blot

miR-155 downregulates MITF-M in LB2201-MEL melanoma cell line.(A) MITF-M and (B) Tyrosinase expression was analyzed by quantitative RT-PCR in melanoma cell line LB2201-MEL 24h and 48h after the transfection with a mimic of miR-155 (30 nM). beta -actin expression was used to normalize MITF-M expression (means ± SD for 3 independent experiments). (C) MITF-M and Tyrosinase protein expression was analyzed by Western Blot in the same cell line 24h and 48h after the transfection with the mimic of miR-155 (30 nM) (1 of 3 independent experiments). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25853464), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse IgG by Western Blot

Detection of Mouse IgG by Western Blot

Quantitative and qualitative analysis of protein synthesis in wild-type and Mstn−/− Tibialis anterior and gastrocnemius muscle using SUnSET.(A-B) Slot-blot western analysis for quantification of protein synthesis in the gastrocnemius and TA muscle. (C) Western blot showing total protein distribution and puromycin labelled polypeptides from wild-type and Mstn−/− TA muscle. Note that some bands remain of similar intensity whereas others decrease. (D) Altered profile of high levels of protein synthesis in IIB fibres of Mstn−/− TA muscle. (E) Immunochistochemistry for the expression of MHC IIB and high levels of protein synthesis determined by puromycin incorporation. (Scale bar = 100μm). (Two-way ANOVA; *p<0.05) Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25807490), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse IgG Horseradish Peroxidase-conjugated Antibody

Application
Recommended Usage

Immunohistochemistry

Use at a 1:20 dilution 

Western Blot

Use at a 1:1000 dilution.

Reviewed Applications

Read 16 reviews rated 4.8 using HAF007 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Sodium Phosphate and Proclin 300.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Do not freeze.
  • 6 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Additional IgG Products

Product Documents for Mouse IgG Horseradish Peroxidase-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse IgG Horseradish Peroxidase-conjugated Antibody

For research use only

Citations for Mouse IgG Horseradish Peroxidase-conjugated Antibody

Customer Reviews for Mouse IgG Horseradish Peroxidase-conjugated Antibody (16)

4.8 out of 5
16 Customer Ratings
5 Stars
81%
4 Stars
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3 Stars
6%
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1 Stars
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Showing  1 - 5 of 16 reviews Showing All
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  • Mouse IgG Horseradish Peroxidase-conjugated Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Cell Lines
    Species: Human
    Verified Customer | Posted 08/05/2025
    Mouse IgG Horseradish Peroxidase-conjugated Antibody HAF007
  • Mouse IgG Horseradish Peroxidase-conjugated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: mouse serum
    Species: Mouse
    Verified Customer | Posted 08/12/2023
    il-6 elisa
    Mouse IgG Horseradish Peroxidase-conjugated Antibody HAF007
  • Mouse IgG Horseradish Peroxidase-conjugated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum
    Species: Mouse
    Verified Customer | Posted 08/12/2023
    ELISA
    Mouse IgG Horseradish Peroxidase-conjugated Antibody HAF007
  • Mouse IgG HRP-conjugated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum
    Species: Human
    Verified Customer | Posted 03/03/2023
    stable and reproducible
    Mouse IgG Horseradish Peroxidase-conjugated Antibody HAF007
  • Mouse IgG HRP-conjugated Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Cell Lysates
    Species: Mouse
    Verified Customer | Posted 07/15/2021
    Mouse IgG Horseradish Peroxidase-conjugated Antibody HAF007
  • Mouse IgG HRP-conjugated Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Cell Lysates
    Species: Mouse
    Verified Customer | Posted 04/29/2021
    Mouse IgG Horseradish Peroxidase-conjugated Antibody HAF007
  • Mouse IgG HRP-conjugated Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HEK293T and NIH3T3 whole cell lysate
    Species: Human
    Verified Customer | Posted 04/15/2021
    Cell lysate was tagged with Tyrosine Mouse Primary antibody. Mouse IgG 2nd antibody works well.
    Mouse IgG Horseradish Peroxidase-conjugated Antibody HAF007
  • Mouse IgG HRP-conjugated Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HEK293 human embryonic kidney cell line and NIH-3T3 mouse embryonic fibroblast cell line
    Species: Human
    Verified Customer | Posted 04/12/2021
    Mouse IgG Horseradish Peroxidase-conjugated Antibody HAF007
  • Mouse IgG HRP-conjugated Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Mv1Lu mink lung epithelial cell line
    Species: Mink
    Verified Customer | Posted 11/20/2019
    used as secondary antibody for WB at 1:1000 with 1hr incubation at RT
    Mouse IgG Horseradish Peroxidase-conjugated Antibody HAF007
  • Mouse IgG HRP-conjugated Antibody
    Name: Danlee Enzler
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Liver cancer tissue
    Species: Mouse
    Verified Customer | Posted 05/01/2018
  • Mouse IgG HRP-conjugated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Recombinant protein
    Species: Human
    Verified Customer | Posted 04/16/2018
    We are using this product for Elisa assay for various human samples, we used at 1:1000 dilution, and it works very well, no artificial background.
  • Mouse IgG HRP-conjugated Antibody
    Name: Stephanie Watkins
    Application: Western Blot
    Sample Tested: T cells
    Species: Human
    Verified Customer | Posted 02/22/2018
    Mouse anti-human beta Actin was used as primary and then R&D Systems goat-anti mouse was used a a secondary (Incubated for one hour at RT in 5% BSA). Membrane was developed using ECL substrate.
    Mouse IgG Horseradish Peroxidase-conjugated Antibody HAF007
  • Mouse IgG HRP-conjugated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: antibody
    Species: Goat
    Verified Customer | Posted 10/20/2017
  • Mouse IgG HRP-conjugated Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Cell Lysates
    Species: Mouse and Human
    Verified Customer | Posted 06/28/2017
    Mouse IgG Horseradish Peroxidase-conjugated Antibody HAF007
  • Mouse IgG Horseradish Peroxidase-conjugated Antibody
    Name: Alexandria Schieber
    Application: Western Blot
    Sample Tested: Adipose tissue and Small intestine
    Species: Mouse
    Verified Customer | Posted 02/22/2016
    Mouse IgG Horseradish Peroxidase-conjugated Antibody HAF007
  • Mouse IgG Horseradish Peroxidase-conjugated Antibody
    Name: Jaiyanth Daniel
    Application: Western Blot
    Sample Tested: Cell Lysates
    Species: E. coli
    Verified Customer | Posted 02/22/2016
    Mouse IgG Horseradish Peroxidase-conjugated Antibody HAF007

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