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Mouse IgG HRP-conjugated Antibody

HAF007 42 Citations 7 Reviews Datasheet / COA / SDS
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HAF007
Detection of Mouse IgG Primary Antibody byWestern Blot
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Product Details
Citations (42)
FAQs
Supplemental Products
Reviews (7)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IgG HRP-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IgG1, IgG2A, IgG2B, and IgG3 heavy and light chains in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross‑reactivity with human IgG and rabbit IgG is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse IgG
Formulation
Sodium Phosphate and Proclin 300.
Label
HRP (Horseradish Peroxidase)

Applications

Recommended Concentration
Sample
Western Blot

Use at a 1:1000 dilution.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Mouse IgG Primary Antibody byWestern Blot View Larger

Detection of Mouse IgG Primary Antibody byWestern Blot Westernblot shows detection of Mouse Anti-Human/Mouse/Rat STAT3 Monoclonal Antibody
(Catalog #MAB1799)using a 1:1000 dilution of HRP-conjugated Anti-Mouse IgG Secondary Antibody
(Catalog # HAF007).  This experiment wasconducted under reducing conditions and using ImmunoblotBuffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Do not freeze.
  • 6 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG

Product Datasheets

Product Datasheet
COA
SDS

Citations for Mouse IgG HRP-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

42 Citations: Showing 1 - 10
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  1. Purification and characterization of a fimbrial protein from Porphyromonas salivosa ATCC 49407
    Authors: Y Koyata, K Watanabe, T Toyama, H Sasaki, N Hamada
    J. Vet. Med. Sci., 2019;0(0):.
    Species: Mouse
    Sample Types: Protein
    Applications: Western Blot
  2. Induction of 3-hydroxy-3-methylglutaryl-CoA reductase mediates statin resistance in breast cancer cells
    Authors: A Göbel, D Breining, M Rauner, LC Hofbauer, TD Rachner
    Cell Death Dis, 2019;10(2):91.
  3. Thioredoxin Reductase-1 Inhibition Augments Endogenous Glutathione-Dependent Antioxidant Responses in Experimental Bronchopulmonary Dysplasia
    Authors: SB Wall, R Wood, K Dunigan, Q Li, R Li, LK Rogers, TE Tipple
    Oxid Med Cell Longev, 2019;2019(0):7945983.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  4. AAV1.NT-3 gene therapy increases muscle fiber diameter through activation of mTOR pathway and metabolic remodeling in a CMT mouse model
    Authors: ME Yalvac, J Amornvit, L Chen, KM Shontz, S Lewis, Z Sahenk
    Gene Ther., 2018;0(0):.
    Species: Mouse
    Sample Types:
    Applications: Western Blot
  5. Bee venom phospholipase A2 ameliorates Alzheimer's disease pathology in A? vaccination treatment without inducing neuro-inflammation in a 3xTg-AD mouse model
    Authors: H Baek, CJ Lee, DB Choi, NS Kim, YS Kim, YJ Ye, YS Kim, JS Kim, I Shim, H Bae
    Sci Rep, 2018;8(1):17369.
  6. Influence of bradykinin B2 receptor and dopamine D2 receptor on the oxidative stress, inflammatory response, and apoptotic process in human endothelial cells
    Authors: A Niewiarows, A Kozik, I Guevara-Lo
    PLoS ONE, 2018;13(11):e0206443.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Microphthalmia-Associated Transcription Factor (MITF) Regulates Immune Cell Migration into Melanoma
    Authors: GM Wiedemann, C Aithal, A Kraechan, C Heise, BL Cadilha, J Zhang, P Duewell, R Ballotti, S Endres, C Bertolotto, S Kobold
    Transl Oncol, 2018;12(2):350-360.
    Species: Mouse
    Sample Types: Cell Lysates
  8. The Parkinson's disease gene product DJ-1 modulates miR-221 to promote neuronal survival against oxidative stress
    Authors: SE Oh, HJ Park, L He, C Skibiel, E Junn, MM Mouradian
    Redox Biol, 2018;19(0):62-73.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. mRNA vaccination with charge-altering releasable transporters elicits human T cell responses and cures established tumors in mice
    Authors: OAW Haabeth, TR Blake, CJ McKinlay, RM Waymouth, PA Wender, R Levy
    Proc. Natl. Acad. Sci. U.S.A., 2018;0(0):.
    Species: Mouse
    Sample Types: Plasma
    Applications: ELISA Development (Secondary)
  10. Liraglutide Treatment Reduces Endothelial Endoplasmic Reticulum Stress and Insulin Resistance in Patients With Diabetes Mellitus
    Authors: R Bretón-Rom, RM Weisbrod, B Feng, M Holbrook, D Ko, MM Stathos, JY Zhang, JL Fetterman, NM Hamburg
    J Am Heart Assoc, 2018;7(18):e009379.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. Allopregnanolone alters follicular and luteal dynamics during the estrous cycle
    Authors: JA Asensio, ARR Cáceres, LT Pelegrina, MLÁ Sanhueza, L Scotti, F Parborell, MR Laconi
    Reprod. Biol. Endocrinol., 2018;16(1):35.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  12. Low-dose paclitaxel downregulates MYC proto-oncogene bHLH transcription factor expression in colorectal carcinoma cells
    Authors: W Li, W Zhu, C Lv, H Qu, K Xu, H Li, H Li, Y Du, G Liu, Y Wang, HJ Wei, HY Zhao
    Oncol Lett, 2018;15(2):1881-1887.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  13. Key Proteins Involved in Spheroid Formation and Angiogenesis in Endothelial Cells After Long-Term Exposure to Simulated Microgravity
    Authors: A Dittrich, D Grimm, J Sahana, J Bauer, M Krüger, M Infanger, NE Magnusson
    Cell. Physiol. Biochem., 2018;45(2):429-445.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  14. Parthenolide reduces gene transcription of prosurvival mediators in U937 cells
    Authors: S Mohammadi, M Zahedpanah, M Nikbakht, M Shaiegan, G Hamidollah, M Nikugoftar, B Rahmani, D Hamedi Asl
    Exp. Oncol., 2017;39(1):30-35.
    Species: Mouse
    Sample Types:
    Applications: Western Blot
  15. Fasting selectively blocks development of acute lymphoblastic leukemia via leptin-receptor upregulation
    Nat. Med, 2016;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  16. Cellular Stress and p53-Associated Apoptosis by Juniperus communis L. Berry Extract Treatment in the Human SH-SY5Y Neuroblastoma Cells
    Int J Mol Sci, 2016;17(7):.
    Applications: Western Blot
  17. The GTPase ARF6 Controls ROS Production to Mediate Angiotensin II-Induced Vascular Smooth Muscle Cell Proliferation.
    Authors: Bourmoum M, Charles R, Claing A
    PLoS ONE, 2016;11(1):e0148097.
  18. Inflammatory Properties and Adjuvant Potential of Synthetic Glycolipids Homologous to Mycolate Esters of the Cell Wall of Mycobacterium tuberculosis
    Authors: Marta Romano
    J Innate Immun, 2016;0(0):.
    Applications: Secondary Antibody
  19. Gamma secretase inhibitor impairs epithelial-to-mesenchymal transition induced by TGF-? in ovarian tumor cell lines
    Authors: M C Pazos
    Mol. Cell. Endocrinol, 2016;440(0):125-137.
    Species: Mouse
    Sample Types: Antibody
    Applications: Western Blot
  20. Decidualization and syndecan-1 knock down sensitize endometrial stromal cells to apoptosis induced by embryonic stimuli.
    Authors: Boeddeker S, Baston-Buest D, Fehm T, Kruessel J, Hess A
    PLoS ONE, 2015;10(4):e0121103.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay
  21. Mucosal pemphigus vulgaris anti-Dsg3 IgG is pathogenic to the oral mucosa of humanized Dsg3 mice.
    Authors: Culton D, McCray S, Park M, Roberts J, Li N, Zedek D, Anhalt G, Cowley D, Liu Z, Diaz L
    J Invest Dermatol, 2015;135(6):1590-7.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  22. Symmorphosis through dietary regulation: a combinatorial role for proteolysis, autophagy and protein synthesis in normalising muscle metabolism and function of hypertrophic mice after acute starvation.
    Authors: Collins-Hooper H, Sartori R, Giallourou N, Matsakas A, Mitchell R, Makarenkova H, Flasskamp H, Macharia R, Ray S, Swann J, Sandri M, Patel K
    PLoS ONE, 2015;10(3):e0120524.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  23. Identification and characterization of PERK activators by phenotypic screening and their effects on NRF2 activation.
    Authors: Xie W, Pariollaud M, Wixted W, Chitnis N, Fornwald J, Truong M, Pao C, Liu Y, Ames R, Callahan J, Solari R, Sanchez Y, Diehl A, Li H
    PLoS ONE, 2015;10(3):e0119738.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  24. microRNA-155, induced by interleukin-1ss, represses the expression of microphthalmia-associated transcription factor (MITF-M) in melanoma cells.
    Authors: Arts N, Cane S, Hennequart M, Lamy J, Bommer G, Van den Eynde B, De Plaen E
    PLoS ONE, 2015;10(4):e0122517.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Neutralization
  25. MicroRNA-7 Promotes Glycolysis to Protect against 1-Methyl-4-phenylpyridinium-induced Cell Death.
    Authors: Chaudhuri A, Kabaria S, Choi D, Mouradian M, Junn E
    J Biol Chem, 2015;290(19):12425-34.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  26. Proangiogenic tumor proteins as potential predictive or prognostic biomarkers for bevacizumab therapy in metastatic colorectal cancer.
    Authors: Bruhn M, Townsend A, Khoon Lee C, Shivasami A, Price T, Wrin J, Arentz G, Tebbutt N, Hocking C, Cunningham D, Hardingham J
    Int J Cancer, 2014;135(3):731-41.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  27. ADAMTS1 inhibits lymphangiogenesis by attenuating phosphorylation of the lymphatic endothelial cell-specific VEGF receptor.
    Authors: Inagaki J, Takahashi K, Ogawa H, Asano K, Faruk Hatipoglu O, Cilek M, Obika M, Ohtsuki T, Hofmann M, Kusachi S, Ninomiya Y, Hirohata S
    Exp Cell Res, 2014;323(2):263-75.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  28. Up-regulation of VEGF by retinoic acid during hyperoxia prevents retinal neovascularization and retinopathy.
    Authors: Wang L, Shi P, Xu Z, Li J, Xie Y, Mitton K, Drenser K, Yan Q
    Invest Ophthalmol Vis Sci, 2014;55(7):4276-87.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  29. HIV and HCV activate the inflammasome in monocytes and macrophages via endosomal Toll-like receptors without induction of type 1 interferon.
    Authors: Chattergoon M, Latanich R, Quinn J, Winter M, Buckheit R, Blankson J, Pardoll D, Cox A
    PLoS Pathog, 2014;10(5):e1004082.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  30. Cell proliferation and apoptosis in rat mammary glands following combinational exposure to bisphenol A and genistein.
    Authors: Wang J, Jenkins S, Lamartiniere C
    BMC Cancer, 2014;14(0):379.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  31. Ligand-independent canonical Wnt activity in canine mammary tumor cell lines associated with aberrant LEF1 expression.
    Authors: Gracanin A, Timmermans-Sprang E, van Wolferen M, Rao N, Grizelj J, Vince S, Hellmen E, Mol J
    PLoS ONE, 2014;9(6):e98698.
    Species: N/A
    Sample Types: N/A
    Applications: Western Blot
  32. Exploring codon optimization and response surface methodology to express biologically active transmembrane RANKL in E. coli.
    Authors: Maharjan S, Singh B, Bok J, Kim J, Jiang T, Cho C, Kang S, Choi Y
    PLoS ONE, 2014;9(5):e96259.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  33. MicroRNA-7 protects against 1-methyl-4-phenylpyridinium-induced cell death by targeting RelA.
    Authors: Choi D, Chae Y, Kabaria S, Chaudhuri A, Jain M, Li H, Mouradian M, Junn E
    J Neurosci, 2014;34(38):12725-37.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  34. Iron-responsive miR-485-3p regulates cellular iron homeostasis by targeting ferroportin.
    Authors: Sangokoya, Carolyn, Doss, Jennifer, Chi, Jen-Tsan
    PLoS Genet, 2013;9(4):e1003408.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  35. Proprotein convertase subtilisin/kexin type 7 (PCSK7) is essential for the zebrafish development and bioavailability of transforming growth factor beta1a (TGFbeta1a).
    Authors: Turpeinen H, Oksanen A, Kivinen V, Kukkurainen S, Uusimaki A, Ramet M, Parikka M, Hytonen V, Nykter M, Pesu M
    J Biol Chem, 2013;288(51):36610-23.
    Species: N/A
    Sample Types: N/A
    Applications: Western Blot
  36. Characterization of proprotein convertase subtilisin/kexin type 9 (PCSK9) trafficking reveals a novel lysosomal targeting mechanism via amyloid precursor-like protein 2 (APLP2).
    Authors: DeVay, Rachel M, Shelton, David L, Liang, Hong
    J Biol Chem, 2013;288(15):10805-18.
    Species: Human
    Sample Types: Cell Lysates
    Applications: ELISA Development
  37. Surface molecule CD229 as a novel target for the diagnosis and treatment of multiple myeloma.
    Authors: Atanackovic D, Panse J, Hildebrandt Y, Jadczak A, Kobold S, Cao Y, Templin J, Meyer S, Reinhard H, Bartels K, Lajmi N, Zander AR, Marx AH, Luetkens T, Bokemeyer C, Kroger N
    Haematologica, 2011;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  38. Anti-inflammatory activity of nanocrystalline silver-derived solutions in porcine contact dermatitis.
    Authors: Nadworny PL, Wang J, Tredget EE
    J Inflamm (Lond), 2010;7(0):13.
    Species: Porcine
    Sample Types: Whole Tissue
    Applications: IHC-P
  39. BAMBI is expressed in endothelial cells and is regulated by lysosomal/autolysosomal degradation.
    Authors: Xavier S, Gilbert V, Rastaldi MP, Krick S, Kollins D, Reddy A, Bottinger E, Cohen CD, Schlondorff D
    PLoS ONE, 2010;5(9):e12995.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  40. Comparison of proteomic and transcriptomic profiles in the bronchial airway epithelium of current and never smokers.
    Authors: Steiling K, Kadar AY, Bergerat A
    PLoS ONE, 2009;4(4):e5043.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  41. Mannan-binding lectin-associated serine protease 3 cleaves synthetic peptides and insulin-like growth factor-binding protein 5.
    Authors: Cortesio CL, Jiang W
    Arch. Biochem. Biophys., 2006;449(1):164-70.
    Species: N/A
    Sample Types: N/A
    Applications: Western Blot
  42. Characterization of cathepsin L secreted by Sf21 insect cells.
    Authors: Johnson GD, Jiang W
    Arch. Biochem. Biophys., 2005;444(1):7-14.
    Species: N/A
    Sample Types: N/A
    Applications: Western Blot

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Reviews for Mouse IgG HRP-conjugated Antibody

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Mouse IgG HRP-conjugated Antibody
By Anonymous on 05/01/2018
Application: ICC/IF Sample Tested: Liver cancer tissue Species: Mouse
Mouse IgG HRP-conjugated Antibody
By Anonymous on 04/16/2018
Application: ELISA Sample Tested: Recombinant protein Species: Human

We are using this product for Elisa assay for various human samples, we used at 1:1000 dilution, and it works very well, no artificial background.

Mouse IgG HRP-conjugated Antibody
By Anonymous on 02/22/2018
Application: WB Sample Tested: T cells Species: Human

Mouse anti-human beta Actin was used as primary and then R&D Systems goat-anti mouse was used a a secondary (Incubated for one hour at RT in 5% BSA). Membrane was developed using ECL substrate.

Western Blot Mouse IgG HRP-conjugated Antibody HAF007
Mouse IgG HRP-conjugated Antibody
By Anonymous on 10/20/2017
Application: ELISA Sample Tested: antibody Species: Goat
Mouse IgG HRP-conjugated Antibody
By Anonymous on 06/28/2017
Application: WB Sample Tested: Cell Lysates Species: Mouse,Human
Western Blot Mouse IgG HRP-conjugated Antibody HAF007
Mouse IgG Horseradish Peroxidase-conjugated Antibody
By Anonymous on 02/22/2016
Application: WB Sample Tested: Adipose tissue,Small intestine Species: Mouse
Western Blot Mouse IgG Horseradish Peroxidase-conjugated Antibody HAF007
Mouse IgG Horseradish Peroxidase-conjugated Antibody
By Anonymous on 02/22/2016
Application: WB Sample Tested: Cell Lysates Species: E. coli
Western Blot Mouse IgG Horseradish Peroxidase-conjugated Antibody HAF007