Human Myocilin Antibody

Catalog # Availability Size / Price Qty
MAB3446
MAB3446-SP
Myocilin in Human Skeletal Muscle.
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Product Details
Citations (14)
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Human Myocilin Antibody Summary

Species Reactivity
Human
Specificity
Detects human Myocilin in direct ELISAs. In direct ELISAs, 75% cross-reactivity with recombinant mouse Myocilin is observed.
Source
Monoclonal Mouse IgG2B Clone # 297817
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Myocilin
Arg33-Met504
Accession # Q99972
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
5-25 µg/mL
Immersion fixed paraffin-embedded sections of Human Skeletal Muscle

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry View Larger

Myocilin in Human Skeletal Muscle. Myocilin was detected in immersion fixed paraffin-embedded sections of Human Skeletal Muscle using Mouse Anti-Human Myocilin Monoclonal Antibody (Catalog # MAB3446) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sarcoplasm and sarcolemma in skeletal muscle cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Myocilin

Myocilin (also known as TIGR) is a variably glycosylated, 65 kDa, secreted polypeptide that belongs to the family of olfactomedin-related proteins. Human Myocilin is synthesized as a 490 aa precursor that contains an 18 aa signal sequence, an 84 aa N-terminus, a 53 aa alpha ‑helical leucine zipper and a 335 aa C-terminus that contains a 260 aa OLF-domain. An alternate start site generates a signal-sequenceless 504 aa mature protein. Myocilin forms nondisulfide-linked dimers and multimers. The human 32 kDa OLF-domain shares 87% aa sequence identity with the OLF-domain in bovine, rat, and mouse.

Entrez Gene IDs
4653 (Human)
Alternate Names
GLC1A; GLC1Amyocilin; GPOA; JOAG; JOAG1; MYOC; Myocilin; myocilin, trabecular meshwork inducible glucocorticoid response; TIGR; TIGRmutated trabecular meshwork-induced glucocorticoid response protein; Trabecular meshwork-induced glucocorticoid response protein

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Citations for Human Myocilin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. Disambiguation of benign and misfolded glaucoma-causing myocilin variants on the basis of protein thermal stability
    Authors: HF Scelsi, KR Hill, BM Barlow, MD Martin, RL Lieberman
    Disease Models & Mechanisms, 2023-01-13;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Calcium dysregulation potentiates wild-type myocilin misfolding: implications for glaucoma pathogenesis
    Authors: Emily G. Saccuzzo, Mackenzie D. Martin, Kamisha R. Hill, Minh Thu Ma, Yemo Ku, Raquel L. Lieberman
    JBIC Journal of Biological Inorganic Chemistry
  3. Anterior Segment Anatomy and Conventional Outflow Physiology of the Tree Shrew (Tupaia belangeri)
    Authors: JV Jasien, AT Read, J van Batenb, KM Perkumas, CR Ethier, WD Stamer, BC Samuels
    Investigative Ophthalmology & Visual Science, 2022-01-03;63(1):21.
    Species: Tree Shrew
    Sample Types: Whole Tissue
    Applications: IHC
  4. Molecular architecture and modifications of full-length myocilin
    Authors: Mackenzie D. Martin, Dustin J. E. Huard, Ricardo C. Guerrero-Ferreira, Ishani M. Desai, Brett M. Barlow, Raquel L. Lieberman
    Experimental Eye Research
  5. Recombinant antibodies recognize conformation-dependent epitopes of the leucine zipper of misfolding-prone myocilin
    Authors: AC Patterson-, AN Qerqez, LR Azouz, MT Ma, SE Hill, Y Ku, LA Schildmeye, JA Maynard, RL Lieberman
    The Journal of Biological Chemistry, 2021-08-09;0(0):101067.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  6. Stem cell transplantation rescued a primary open-angle glaucoma mouse model
    Authors: Siqi Xiong, Ajay Kumar, Shenghe Tian, Eman E Taher, Enzhi Yang, Paul R Kinchington et al.
    eLife
  7. Assessment of differential intraocular pressure response to dexamethasone treatment in perfusion cultured Indian cadaveric eyes
    Authors: R Haribalaga, C Gowri Priy, R Sharmila, S Krishnadas, V Muthukkaru, CE Willoughby, S Senthilkum
    Scientific Reports, 2021-01-12;11(1):605.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  8. Isolation and Characterization of primary human trabecular meshwork cells from segmental flow regions: New tools for understanding segmental flow
    Authors: Julia Staverosky, Kamesh Dhamodaran, Ted Acott, VijayKrishna Raghunathan, Janice Vranka
    Experimental Eye Research
  9. Stable calcium-free myocilin olfactomedin domain variants reveal challenges in differentiating between�benign and glaucoma-causing mutations
    Authors: SE Hill, MS Kwon, MD Martin, A Suntharali, A Hazel, CA Dickey, JC Gumbart, RL Lieberman
    J. Biol. Chem., 2019-07-02;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Identification of myocilin as a blood plasma protein and analysis of its role in leukocyte adhesion to endothelial cell monolayers
    Authors: José-Daniel Aroca-Aguilar, Ana Fernández-Navarro, Jesús Ontañón, Miguel Coca-Prados, Julio Escribano
    PLOS ONE
  11. Epitope mapping of commercial antibodies that detect myocilin
    Authors: Athéna C. Patterson-Orazem, Shannon E. Hill, Michael P. Fautsch, Raquel L. Lieberman
    Experimental Eye Research
  12. Consensus recommendations for trabecular meshwork cell isolation, characterization and culture
    Authors: Kate E. Keller, Sanjoy K. Bhattacharya, Theresa Borrás, Thomas M. Brunner, Sunee Chansangpetch, Abbott F. Clark et al.
    Experimental Eye Research
  13. Glucose-regulated protein 94 triage of mutant myocilin through endoplasmic reticulum-associated degradation subverts a more efficient autophagic clearance mechanism.
    Authors: Suntharalingam A, Abisambra J, O'Leary J, Koren J, Zhang B, Joe M, Blair L, Hill S, Jinwal U, Cockman M, Duerfeldt A, Tomarev S, Blagg B, Lieberman R, Dickey C
    J Biol Chem, 2012-10-03;287(48):40661-9.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  14. Expression of myocilin mutants sensitizes cells to oxidative stress-induced apoptosis: implication for glaucoma pathogenesis.
    Authors: Joe MK, Tomarev SI
    Am. J. Pathol., 2010-04-09;176(6):2880-90.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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