Human Myocilin Antibody
Human Myocilin Antibody Summary
Arg33-Met504
Accession # Q99972
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Myocilin in Human Skeletal Muscle. Myocilin was detected in immersion fixed paraffin-embedded sections of Human Skeletal Muscle using Mouse Anti-Human Myocilin Monoclonal Antibody (Catalog # MAB3446) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sarcoplasm and sarcolemma in skeletal muscle cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Myocilin
Myocilin (also known as TIGR) is a variably glycosylated, 65 kDa, secreted polypeptide that belongs to the family of olfactomedin-related proteins. Human Myocilin is synthesized as a 490 aa precursor that contains an 18 aa signal sequence, an 84 aa N-terminus, a 53 aa alpha ‑helical leucine zipper and a 335 aa C-terminus that contains a 260 aa OLF-domain. An alternate start site generates a signal-sequenceless 504 aa mature protein. Myocilin forms nondisulfide-linked dimers and multimers. The human 32 kDa OLF-domain shares 87% aa sequence identity with the OLF-domain in bovine, rat, and mouse.
Product Datasheets
Citations for Human Myocilin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 7
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Disambiguation of benign and misfolded glaucoma-causing myocilin variants on the basis of protein thermal stability
Authors: HF Scelsi, KR Hill, BM Barlow, MD Martin, RL Lieberman
Disease Models & Mechanisms, 2022;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Anterior Segment Anatomy and Conventional Outflow Physiology of the Tree Shrew (Tupaia belangeri)
Authors: JV Jasien, AT Read, J van Batenb, KM Perkumas, CR Ethier, WD Stamer, BC Samuels
Investigative Ophthalmology & Visual Science, 2022;63(1):21.
Species: Tree Shrew
Sample Types: Whole Tissue
Applications: IHC -
Recombinant antibodies recognize conformation-dependent epitopes of the leucine zipper of misfolding-prone myocilin
Authors: AC Patterson-, AN Qerqez, LR Azouz, MT Ma, SE Hill, Y Ku, LA Schildmeye, JA Maynard, RL Lieberman
The Journal of Biological Chemistry, 2021;0(0):101067.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Assessment of differential intraocular pressure response to dexamethasone treatment in perfusion cultured Indian cadaveric eyes
Authors: R Haribalaga, C Gowri Priy, R Sharmila, S Krishnadas, V Muthukkaru, CE Willoughby, S Senthilkum
Scientific Reports, 2021;11(1):605.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Stable calcium-free myocilin olfactomedin domain variants reveal challenges in differentiating between�benign and glaucoma-causing mutations
Authors: SE Hill, MS Kwon, MD Martin, A Suntharali, A Hazel, CA Dickey, JC Gumbart, RL Lieberman
J. Biol. Chem., 2019;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Glucose-regulated protein 94 triage of mutant myocilin through endoplasmic reticulum-associated degradation subverts a more efficient autophagic clearance mechanism.
Authors: Suntharalingam A, Abisambra J, O'Leary J, Koren J, Zhang B, Joe M, Blair L, Hill S, Jinwal U, Cockman M, Duerfeldt A, Tomarev S, Blagg B, Lieberman R, Dickey C
J Biol Chem, 2012;287(48):40661-9.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation -
Expression of myocilin mutants sensitizes cells to oxidative stress-induced apoptosis: implication for glaucoma pathogenesis.
Authors: Joe MK, Tomarev SI,
Am. J. Pathol., 2010;176(6):2880-90.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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