Mouse IL-10 Antibody

(27 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse IL-10 in sandwich ELISAs and Western blots. In sandwich ELISAs, less than 8% cross-reactivity with recombinant rat IL-10 and 1% cross-reactivity with recombinant cotton rat IL‑10 is observed. In sandwich ELISAs, no cross-reactivity with recombinant human (rh) IL-10, recombinant mouse IL-10 R, rhIL‑10 R, recombinant canine IL-10, recombinant feline IL-10, or recombinant porcine IL-10 is observed.
  • Source
    Monoclonal Rat IgG1 Clone # JES052A5
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant mouse IL-10
    Accession # P18893
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
    • Mouse IL-10 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Mouse IL‑10 Antibody (Catalog # MAB417)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Mouse IL‑10 Biotinylated Antibody (Catalog # BAF417)
  • ELISA Standard
     
    Recombinant Mouse IL-10 Protein (Catalog # 417-ML)
  • Neutralization
    Measured by its ability to neutralize IL‑10-induced proliferation in the MC/9‑2 mouse mast cell line. Thompson-Snipes, L.  et al. (1991) J. Exp. Med. 173:507. The Neutralization Dose (ND50) is typically 0.005-0.015 µg/mL in the presence of 2.5 ng/mL Recombinant Mouse IL‑10.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Recombinant Mouse IL‑10 by Western Blot. Western blot shows 25 ng of Recombinant Mouse IL‑10 (Catalog #
417-ML), Recombinant Human IL‑10 (Catalog # 217-IL), Recombinant Rat IL‑10 (Catalog # 522-RLB), and Recombinant Human IL‑26/AK155 Monomer (Catalog # 1375-IL). PVDF Membrane was probed with 1 µg/mL of Rat Anti-Mouse IL‑10 Monoclonal Antibody (Catalog # MAB417) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IL‑10 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Cell Proliferation Induced by IL‑10 and Neutralization by Mouse IL‑10 Antibody. Recombinant Mouse IL‑10 (Catalog # 417-ML) stimulates proliferation in the MC/9‑2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑10 (2.5 ng/mL) is neutralized (green line) by increasing concen­trations of Rat Anti-Mouse IL‑10 Monoclonal Antibody (Catalog # MAB417). The ND50 is typically 0.005-0.015 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-10
Interleukin 10 (IL-10) is a pleiotropic cytokine that can exert either immunostimulatory or immunosuppressive effects on a variety of cell types. It is produced predominantly by Th2 lymphocytes and other activated lymphoid cells.
  • Long Name:
    Interleukin 10
  • Entrez Gene IDs:
    3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 493683 (Feline); 100135553 (Guinea Pig); 2949786 (Viral)
  • Alternate Names:
    CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

27 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. IL-33 contributes to sepsis-induced long-term immunosuppression by expanding the regulatory T cell population
    Authors: DC Nascimento, PH Melo, AR Piñeros, RG Ferreira, DF Colón, PB Donate, FV Castanheir, A Gozzi, PG Czaikoski, W Niedbala, MC Borges, DS Zamboni, FY Liew, FQ Cunha, JC Alves-Filh
    Nat Commun, 2017;8(0):14919.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Functional Assay
  2. CCR7 Deficiency Allows Accelerated Clearance of Chlamydia from the Female Reproductive Tract
    Authors: LX Li, JC Labuda, DM Imai, SM Griffey, SJ McSorley
    J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Capture
  3. A critical role for IRF5 in regulating allergic airway inflammation
    Authors: AJ Byrne, M Weiss, SA Mathie, SA Walker, HL Eames, D Saliba, CM Lloyd, IA Udalova
    Mucosal Immunol, 2017;10(3):716-726.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: ELISA Capture
  4. Enhanced immunoregulation of mesenchymal stem cells by IL-10-producing type 1 regulatory T cells in collagen-induced arthritis
    Authors: Jung-Yeon Lim
    Sci Rep, 2016;6(0):26851.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Capture
  5. Neuropathic pain is constitutively suppressed in early life by anti-inflammatory neuroimmune regulation.
    Authors: McKelvey R, Berta T, Old E, Ji R, Fitzgerald M
    J Neurosci, 2015;35(2):457-66.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  6. Myeloid cell TRAF3 regulates immune responses and inhibits inflammation and tumor development in mice.
    Authors: Lalani A, Moore C, Luo C, Kreider B, Liu Y, Morse H, Xie P
    J Immunol, 2015;194(1):334-48.
    Species: Mouse
    Sample Type: Serum
    Application: ELISA Development
  7. M2b macrophage elimination and improved resistance of mice with chronic alcohol consumption to opportunistic infections.
    Authors: Ohama H, Asai A, Ito I, Suzuki S, Kobayashi M, Higuchi K, Suzuki F
    Am J Pathol, 2015;185(2):420-31.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Functional Assay
  8. IL-33-mediated protection against experimental cerebral malaria is linked to induction of type 2 innate lymphoid cells, M2 macrophages and regulatory T cells.
    Authors: Besnard A, Guabiraba R, Niedbala W, Palomo J, Reverchon F, Shaw T, Couper K, Ryffel B, Liew F
    PLoS Pathog, 2015;11(2):e1004607.
    Species: Mouse
    Sample Type: In Vivo
  9. TLR9 ligands induce S100A8 in macrophages via a STAT3-dependent pathway which requires IL-10 and PGE2.
    Authors: Hsu, Kenneth, Chung, Yuen Min, Endoh, Yasumi, Geczy, Carolyn
    PLoS ONE, 2014;9(8):e103629.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  10. Delayed wound healing due to increased interleukin-10 expression in mice with lymphatic dysfunction.
    Authors: Kimura T, Sugaya M, Blauvelt A, Okochi H, Sato S
    J Leukoc Biol, 2013;94(1):137-45.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  11. Response patterns of cytokines/chemokines in two murine strains after irradiation.
    Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
    Cytokine, 2012;58(2):169-77.
    Species: Mouse
    Sample Type: Plasma
    Application: Luminex Assay Development
  12. Interleukin-10 haplotype may predict survival and relapse in resected non-small cell lung cancer.
    Authors: Wang YC, Sung WW, Wu TC, Wang L, Chien WP, Cheng YW, Chen CY, Shieh SH, Lee H
    PLoS ONE, 2012;7(7):e39525.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  13. Treatment failure of a TLR-7 agonist occurs due to self-regulation of acute inflammation and can be overcome by IL-10 blockade.
    Authors: Lu H, Wagner WM, Gad E, Yang Y, Duan H, Amon LM, Van Denend N, Larson ER, Chang A, Tufvesson H, Disis ML
    J. Immunol., 2010;184(9):5360-7.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  14. ST2 deficient mice display a normal host defense against pulmonary infection with Mycobacterium tuberculosis.
    Authors: Wieland CW, van der Windt GJ, Florquin S, McKenzie AN, van der Poll T
    Microbes Infect., 2009;11(4):524-30.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  15. Tr1 regulatory T cells induced by ConA pretreatment prevent mice from ConA-induced hepatitis.
    Authors: Ye F, Yan S, Xu L, Jiang Z, Liu N, Xiong S, Wang Y, Chu Y
    Immunol. Lett., 2009;122(2):198-207.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  16. CD14 contributes to pulmonary inflammation and mortality during murine tuberculosis.
    Authors: Wieland CW, van der Windt GJ, Wiersinga WJ, Florquin S, van der Poll T
    Immunology, 2008;125(2):272-9.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: ELISA Development
  17. CXCL12 (SDF-1alpha) suppresses ongoing experimental autoimmune encephalomyelitis by selecting antigen-specific regulatory T cells.
    Authors: Meiron M, Zohar Y, Anunu R, Wildbaum G, Karin N
    J. Exp. Med., 2008;205(11):2643-55.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  18. Tumor-specific Th17-polarized cells eradicate large established melanoma.
    Authors: Muranski P, Boni A, Antony PA, Cassard L, Irvine KR, Kaiser A, Paulos CM, Palmer DC, Touloukian CE, Ptak K, Gattinoni L, Wrzesinski C, Hinrichs CS, Kerstann KW, Feigenbaum L, Chan CC, Restifo NP
    Blood, 2008;112(2):362-73.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  19. Induction of antigen-specific regulatory T lymphocytes by human dendritic cells expressing the glucocorticoid-induced leucine zipper.
    Authors: Hamdi H, Godot V, Maillot MC, Prejean MV, Cohen N, Krzysiek R, Lemoine FM, Zou W, Emilie D
    Blood, 2007;110(1):211-9.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  20. Relative importance of T-cell subsets in monocytotropic ehrlichiosis: a novel effector mechanism involved in Ehrlichia-induced immunopathology in murine ehrlichiosis.
    Authors: Ismail N, Crossley EC, Stevenson HL, Walker DH
    Infect. Immun., 2007;75(9):4608-20.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  21. Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality.
    Authors: Osuchowski MF, Welch K, Siddiqui J, Remick DG
    J. Immunol., 2006;177(3):1967-74.
    Species: Mouse
    Sample Type: Plasma
    Application: ELISA Development
  22. Lack of in vitro and in vivo recognition of Francisella tularensis subspecies lipopolysaccharide by Toll-like receptors.
    Authors: Hajjar AM, Harvey MD, Shaffer SA, Goodlett DR, Sjostedt A, Edebro H, Forsman M, Bystrom M, Pelletier M, Wilson CB, Miller SI, Skerrett SJ, Ernst RK
    Infect. Immun., 2006;74(12):6730-8.
    Species: Human
    Sample Type: BALF
    Application: ELISA Development
  23. Severe sepsis exacerbates cell-mediated immunity in the lung due to an altered dendritic cell cytokine profile.
    Authors: Wen H, Hogaboam CM, Gauldie J, Kunkel SL
    Am. J. Pathol., 2006;168(6):1940-50.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: ELISA Development
  24. Sequential ELISA to profile multiple cytokines from small volumes.
    Authors: Osuchowski MF, Siddiqui J, Copeland S, Remick DG
    J. Immunol. Methods, 2005;302(1):172-81.
    Species: Mouse
    Sample Type: Plasma
    Application: ELISA Development
  25. Selective macrophage suppression during sepsis.
    Authors: Ellaban E, Bolgos G, Remick D
    Cell. Immunol., 2004;231(1):103-11.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  26. Nonlymphoid peritoneal cells suppress the T cell response to Mls.
    Authors: Rosini L, Matlack R, Taylor J, Howell KF, Yeh K, Pennello A, Riggs JE
    Immunobiology, 2004;209(8):575-84.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  27. Attenuation of autoimmune disease in Fas-deficient mice by treatment with a cytotoxic benzodiazepine.
    Authors: Bednarski JJ, Warner RE, Rao T
    Arthritis Rheum., 2003;48(3):757-66.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ELISpot Development
Expand to show all 27 Citations
Isotype Controls
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Rat IgG1 Isotype Control

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Normal Rat IgG Control

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Rat IgG1 Isotype Control

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Secondary Antibodies
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Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
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Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL013 2
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Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Flow F0113 2  
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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 2  
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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
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Goat Anti-Rat IgG Antibody

WB AF005 1
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
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Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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