Detection of Recombinant Mouse IL‑10 by Western Blot. Western blot shows 25 ng of Recombinant Mouse IL‑10 (Catalog #|
417-ML), Recombinant Human IL‑10 (Catalog # 217-IL), Recombinant Rat IL‑10 (Catalog # 522-RLB), and Recombinant Human IL‑26/AK155 Monomer (Catalog # 1375-IL). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Mouse IL‑10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-417-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IL‑10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Cell Proliferation Induced by IL‑10 and Neutralization by Mouse IL‑10 Antibody. |
Recombinant Mouse IL‑10 (Catalog # 417-ML) stimulates proliferation in the MC/9‑2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑10 (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL‑10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-417-NA). The ND50 is typically 0.01‑0.05 µg/mL.
Interleukin 10, also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 family of alpha -helical cytokines that also includes IL-19, IL‑20, IL-22, and IL-24. IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts. Mature mouse IL-10 shares 85% amino acid sequence identity with rat and 70%‑77% with bovine, canine, equine, feline, human, ovine, and porcine IL-10. Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells. IL-10 is a 178 amino acid molecule that contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently associated homodimer. The IL-10 dimer binds to two IL-10 R alpha /IL-10 R1 chains, resulting in recruitment of two IL‑10 R beta /IL‑10 R2 chains and activation of a signaling cascade involving JAK1, TYK2, and STAT3. IL-10 R beta does not bind IL-10 by itself but is required for signal transduction. IL-10 R beta also associates with IL-20 R alpha, IL-22 R alpha, or IL-28 R alpha to form the receptor complexes for IL‑22, IL-26, IL-28, and IL-29. IL-10 is a critical molecule in the control of viral infections and allergic and autoimmune inflammation. It promotes phagocytic uptake and Th2 responses but suppresses antigen presentation and Th1 proinflammatory responses.
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