Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Neutralization

Cited:

Western Blot, Neutralization

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all IL-10 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse IL‑10
Ser19-Ser178
Accession # NP_034678

Specificity

Detects mouse IL-10 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant rat IL‑10 and recombinant cotton rat IL-10 is observed, and less than 5% cross-reactivity with recombinant porcine IL-10, recombinant canine IL-10, recombinant feline IL-10, recombinant equine IL-10, recombinant viral IL-10, recombinant guinea pig IL-10, and recombinant human IL‑10 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse IL‑10 Antibody

Detection of Recombinant Mouse IL-10 antibody by Western Blot.

Detection of Recombinant Mouse IL‑10 by Western Blot.

Western blot shows 25 ng of Recombinant Mouse IL-10 (Catalog # 417-ML), Recombinant Human IL-10 (Catalog # 217-IL), Recombinant Rat IL-10 (Catalog # 522-RLB), and Recombinant Human IL-26/AK155 Monomer (Catalog # 1375-IL). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Mouse IL-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-417-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IL-10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Cell Proliferation Induced by IL‑10 and Neutralization by Mouse IL‑10 Antibody.

Cell Proliferation Induced by IL‑10 and Neutralization by Mouse IL‑10 Antibody.

Recombinant Mouse IL-10 (Catalog # 417-ML) stimulates proliferation in the MC/9-2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-10 (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-417-NA). The ND50 is typically 0.01-0.05 µg/mL.

Applications for Mouse IL‑10 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse IL‑10 (Catalog # 417-ML)

Neutralization

Measured by its ability to neutralize IL‑10-induced proliferation in the MC/9‑2 mouse mast cell line. Thompson-Snipes, L.  et al. (1991) J. Exp. Med. 173:507. The Neutralization Dose (ND50) is typically 0.01-0.05 µg/mL in the presence of 2.5 ng/mL Recombinant Mouse IL‑10.

Reviewed Applications

Read 1 review rated 5 using AF-417-NA in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-10

Interleukin 10, also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 family of alpha -helical cytokines that also includes IL-19, IL‑20, IL-22, and IL-24. IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts. Mature mouse IL-10 shares 85% amino acid sequence identity with rat and 70%‑77% with bovine, canine, equine, feline, human, ovine, and porcine IL-10. Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells. IL-10 is a 178 amino acid molecule that contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently associated homodimer. The IL-10 dimer binds to two IL-10 R alpha /IL-10 R1 chains, resulting in recruitment of two IL‑10 R beta /IL‑10 R2 chains and activation of a signaling cascade involving JAK1, TYK2, and STAT3. IL-10 R beta does not bind IL-10 by itself but is required for signal transduction. IL-10 R beta also associates with IL-20 R alpha, IL-22 R alpha, or IL-28 R alpha to form the receptor complexes for IL‑22, IL-26, IL-28, and IL-29. IL-10 is a critical molecule in the control of viral infections and allergic and autoimmune inflammation. It promotes phagocytic uptake and Th2 responses but suppresses antigen presentation and Th1 proinflammatory responses.

Long Name

Interleukin 10

Alternate Names

CSIF, GVHDS, IL10, IL10A, TGIF

Entrez Gene IDs

3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 102133450 (Cynomolgus Monkey); 100034187 (Equine); 493683 (Feline); 100715618 (Guinea Pig); 2949786 (Viral)

Gene Symbol

IL10

UniProt

NP_034678

Additional IL-10 Products

Product Documents for Mouse IL‑10 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse IL‑10 Antibody

For research use only

Citations for Mouse IL‑10 Antibody

Customer Reviews for Mouse IL‑10 Antibody (1)

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  • Mouse IL-10 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Aorta tissue
    Species: Mouse
    Verified Customer | Posted 04/06/2021
    Mouse IL‑10 Antibody AF-417-NA

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