Mouse IL-13 R alpha 2 Antibody

Catalog # Availability Size / Price Qty
AF539
AF539-SP
Mouse IL-13 R alpha 2 Antibody in Data
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Product Details
Citations (8)
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Mouse IL-13 R alpha 2 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-13 R alpha 2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human (rh) IL-13 R alpha 2 is observed and less than 1% cross-reactivity with rhIL-5R alpha, rhIL-5R beta, rhIL-9 R, and rhIL-4 R is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IL‑13 R alpha 2
Leu22-Lys334
Accession # O88786
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse IL‑13 R alpha 2 Fc Chimera (Catalog # 539-IR)
Immunohistochemistry
5-15 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunocytochemistry IL‑13 R alpha 2 in Mouse Splenocytes. View Larger

IL‑13 R alpha 2 in Mouse Splenocytes. IL-13 Ra2 was detected in immersion fixed mouse splenocytes using Mouse IL-13 Ra2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF539) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry IL‑13 R alpha 2 in Mouse Thymus. View Larger

IL‑13 R alpha 2 in Mouse Thymus. IL-13 Ra2 was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse IL-13 Ra2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF539) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-13 R alpha 2

Two type1 membrane proteins belonging to the hemopoietin receptor family have been cloned and shown to bind IL-13 with differing affinities. The lower affinity IL-13 binding protein, previously designated IL-13 R alpha, IL-13 R alpha ' or NR4, is now referred to as IL-13 R alpha 1. The high affinity IL-13 binding protein, previously also designated IL-13 R or IL-13 R alpha ', is now referred to as IL-13 R alpha 2.

The mouse IL-13 R alpha 2 cDNA encodes a 383 amino acid (aa) residue precursor protein with a putative 21 aa residue signal peptide, a 313 residue extracellular domain, a 22 aa residue transmembrane region, and a 27 aa residue cytoplasmic tail. Human and mouse IL-13 R alpha 2 share 59% aa sequence identity. The extracellular domain of IL-13 R alpha 2 is also closely related to that of IL-13 R alpha 1. However, the cytoplasmic domain of IL-13 R alpha 2 lacks the box 1 and box 2 signaling motif and is much shorter than that of IL-13 R alpha 1, suggesting that the two receptors are functionally distinct. IL-13 R alpha 1 has been shown to combine with IL-4 R alpha to form a high-affinity receptor complex capable of transducing both an IL-4-dependent and an IL-13-dependent proliferative signal. The role of IL-13 R alpha 2 in IL-13 signaling remains to be elucidated. The amino-terminal 27 amino acid residues of mouse IL-13 R alpha 2 are identical to that of a soluble mouse IL-13 binding protein purified from mouse serum and urine. Recombinant mouse IL-13 R alpha /Fc chimera has been shown to bind IL-13 with high affinity and is a potent IL-13 antagonist.

References
  1. Donaldson, D.D. et al. (1998) J. Immunol. 161:2317.
  2. Chomarat, P. and J. Banchereau (1998) Int. Rev. Immunol. 17:1.
Long Name
Interleukin 13 Receptor alpha 2
Entrez Gene IDs
3598 (Human); 16165 (Mouse)
Alternate Names
cancer/testis antigen 19; CD213a2 antigen; CD213a2; CT19; IL-13 R alpha 2; IL-13 receptor subunit alpha-2; IL13BP; IL13R alpha 2; IL-13R subunit alpha-2; IL13R; IL-13R; IL13RA2; IL-13Ra2; IL-13R-alpha-2; interleukin 13 binding protein; interleukin 13 receptor alpha 2 chain; interleukin 13 receptor, alpha 2; interleukin-13 receptor subunit alpha-2; Interleukin-13-binding protein

Product Datasheets

Citations for Mouse IL-13 R alpha 2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. BCG vaccination reduces the mortality of Mycobacterium tuberculosis-infected type 2 diabetes mellitus mice
    Authors: RK Radhakrish, RS Thandi, D Tripathi, P Paidipally, MK McAllister, S Mulik, B Samten, R Vankayalap
    JCI Insight, 2020;5(5):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Unexpected involvement of IL-13 signalling via a STAT6 independent mechanism during murine IgG2a development following viral vaccination
    Authors: MA Hamid, RJ Jackson, S Roy, M Khanna, C Ranasinghe
    Eur. J. Immunol., 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Disruption of the TWEAK/Fn14 pathway prevents 5-fluorouracil-induced diarrhea in mice
    Authors: T Sezaki, Y Hirata, T Hagiwara, YI Kawamura, T Okamura, R Takanashi, K Nakano, M Tamura-Nak, LC Burkly, T Dohi
    World J. Gastroenterol., 2017;23(13):2294-2307.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  4. GdCl3 Attenuates Schistosomiasis japonicum Egg-Induced Granulomatosis Accompanied by Decreased Macrophage Infiltration in Murine Liver.
    Authors: Zheng S, Lu Q, Xu Y, Wang X, Shen J, Wang W
    PLoS ONE, 2015;10(8):e0132222.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  5. Matrix metalloproteinase 8 contributes to solubilization of IL-13 receptor alpha2 in vivo.
    Authors: Chen W, Tabata Y, Gibson AM, Daines MO, Warrier MR, Wills-Karp M, Hershey GK
    J. Allergy Clin. Immunol., 2008;122(3):625-32.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  6. Level of expression of IL-13R alpha 2 impacts receptor distribution and IL-13 signaling.
    Authors: Daines MO, Tabata Y, Walker BA, Chen W, Warrier MR, Basu S, Hershey GK
    J. Immunol., 2006;176(12):7495-501.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Loss of pro-apoptotic Bim promotes accumulation of pulmonary T lymphocytes and enhances allergen-induced goblet cell metaplasia.
    Authors: Pierce J, Rir-Sima-Ah J, Estrada I, Wilder J, Strasser A, Tesfaigzi Y
    Am. J. Physiol. Lung Cell Mol. Physiol., 2006;291(5):L862-70.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  8. Impact of interleukin-13 responsiveness on the synthetic and proliferative properties of Th1- and Th2-type pulmonary granuloma fibroblasts.
    Authors: Jakubzick C, Choi ES, Kunkel SL, Joshi BH, Puri RK, Hogaboam CM
    Am. J. Pathol., 2003;162(5):1475-86.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC

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