Mouse IL-17E/IL-25 Antibody

Catalog # Availability Size / Price Qty
AF1399
AF1399-SP
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews (1)

Mouse IL-17E/IL-25 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-17E/IL-25 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross‑reactivity with recombinant human (rh) IL-17E and recombinant rat IL-17E is observed, and less than 1% cross‑reactivity with recombinant mouse (rm) IL-17, rmIL-17B, rhIL-17C, rhIL-17D, and rhIL‑17F is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse IL‑17E/IL-25
Val17-Ala169
Accession # NP_542767
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse IL-17E/IL-25 (Catalog # 1399-IL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-17E/IL-25

The Interleukin 17 (IL-17) family proteins, comprising six members (IL-17, IL-17B through IL-17F), are secreted, structurally related proteins that share a conserved cysteine-knot fold near the C-terminus, but have considerable sequence divergence at the N-terminus. With the exception of IL-17B, which exists as a non-covalently linked dimer, all IL-17 family members are disulfide-linked dimers. IL-17 family proteins are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions (1, 2).

Mouse IL-17E cDNA encodes a 169 amino acid residues (aa) precursor protein with a putative 16 aa signal peptide (5). Mature mouse IL-17E shares 76% and 91% amino sequence (aa) identity with mature human and rat IL-17E, respectively. Mouse IL-17E also shares from 24% to 32% sequence identity with the other mouse IL‑17 family members. IL-17E expression was detected at very low levels by PCR in various peripheral tissues including brain, kidney, lung, prostate, testis, adrenal gland spinal cord and trachea. IL-17E binds and activates IL-17 B Receptor (IL-17B R) (alternatively known as IL-17 Rh1, IL-17E R, and EVI27) (3, 4), which is expressed in kidney and liver, and at lower levels in brain, testis and other endocrine tissues. The expression of IL-17B R is up regulated under inflammatory conditions. Ligation of IL-17E to IL-17 RB induces activation of nuclear factor kappa-B and stimulates the production of the proinflamatory cytokine IL-8 (3). IL-17 has also been found to promote the expression of the prototypical Th2 genes (4, 5).

References
  1. Aggarwal, S. and A.L. Gurney (2002) J. Leukoc. Biol. 71:1.
  2. Moseley, T.A. et al. (2003) Cytokine & Growth Factor Rev. 14:155.
  3. Lee, J. et al. (2001) J. Biol. Chem. 276:1660.
  4. Hurst, S.D. et al. (2002) J. Immunol. 169:443.
  5. Pan, G. et al. (2001) J. Immunol. 167:6569.
Long Name
Interleukin 17E
Entrez Gene IDs
64806 (Human); 140806 (Mouse); 501996 (Rat)
Alternate Names
IL17E; IL-17E; IL25; IL-25; interleukin 25; Interleukin-17E; interleukin-25

Product Datasheets

Citations for Mouse IL-17E/IL-25 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Thymic tuft cells promote an IL-4-enriched medulla and shape thymocyte development
    Authors: CN Miller, I Proekt, J von Moltke, KL Wells, AR Rajpurkar, H Wang, K Rattay, IS Khan, TC Metzger, JL Pollack, AC Fries, WW Lwin, EJ Wigton, AV Parent, B Kyewski, DJ Erle, KA Hogquist, LM Steinmetz, RM Locksley, MS Anderson
    Nature, 2018;559(7715):627-631.
    Species: Mouse
    Sample Types: Protein
    Applications: Radioimmunoassay (RIA)
  2. Critical role of IL-25 in nematode infection-induced alterations in intestinal function.
    Authors: Zhao A, Urban JF, Sun R
    J. Immunol., 2010;185(0):6921-9.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Mouse IL-17E/IL-25 Antibody

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Mouse IL-17E/IL-25 Antibody
By Anonymous on 10/06/2019
Application: ICC/IF Sample Tested: Small intestine Species: Mouse

RnD Systems Response: Thank you for reviewing our product. We are sorry to that that this antibody did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.