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Mouse IL-1 beta /IL-1F2 Biotinylated Antibody

Catalog #: BAF401
30 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
BAF401

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Citations (30)
FAQs
Supplemental Products
Reviews (1)
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IL-1 beta /IL-1F2 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-1 beta in ELISAs and Western blots. In sandwich immunoassays, less than 4% cross-reactivity with recombinant rat (rr) IL-1 beta is observed and less than 0.05% cross-reactivity with rhIL-1 beta and rmIL-1 alpha is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse IL-1 beta (R&D Systems, Catalog # 401-ML)
Val118-Ser269
Accession # NP_032387
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse IL‑1 beta /IL‑1F2 (Catalog # 401-ML)

Mouse IL-1 beta /IL-1F2 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Mouse IL‑1 beta /IL‑1F2 Antibody (Catalog # MAB401)

Standard: Recombinant Mouse IL-1 beta/IL-1F2 Protein (Catalog # 401-ML)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-1 beta/IL-1F2

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2, IL1B), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 17% amino acid (aa) identity in mouse. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. The 17 kDa molecular weight mature mouse IL-1 beta shares 90% aa sequence identity with cotton rat and rat and 67%-78% with canine, equine, feline, human, porcine, and rhesus macaque IL-1 beta. IL-1 beta functions in a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases.

Long Name
Interleukin 1 beta
Entrez Gene IDs
3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
Alternate Names
catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta

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Citations for Mouse IL-1 beta /IL-1F2 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

30 Citations: Showing 1 - 10
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  1. Assessing the Association of Mitochondrial Function and Inflammasome Activation in Murine Macrophages Exposed to Select Mitotoxic Tri-Organotin Compounds
    Authors: GM Childers, CA Perry, B Blachut, N Martin, CD Bortner, S Sieber, JL Li, MB Fessler, GJ Harry
    Environmental health perspectives, 2021-04-30;129(4):47015.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Gasdermin D mediates the maturation and release of IL-1&alpha downstream of inflammasomes
    Authors: K Tsuchiya, S Hosojima, H Hara, H Kushiyama, MR Mahib, T Kinoshita, T Suda
    Cell Reports, 2021-03-23;34(12):108887.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Peripheral Taenia infection increases immunoglobulins in the central nervous system
    Authors: S Van Belle, A de Lange, H Tomes, R Lucas, V Naidoo, JV Raimondo
    International journal for parasitology, 2021-03-20;51(8):685-692.
    Species: Mouse
    Sample Types: Tissue Homogenates
  4. Interleukin (IL)-25 suppresses IL-22-induced osteoclastogenesis in rheumatoid arthritis via STAT3 and p38 MAPK/I&kappaB&alpha pathway
    Authors: HK Min, JY Won, BM Kim, KA Lee, SJ Lee, SH Lee, HR Kim, KW Kim
    Arthritis Research & Therapy, 2020-09-23;22(1):222.
    Species: Human
    Sample Types: Synovial Fluid
  5. &beta-Hydroxybutyrate inhibits inflammasome activation to attenuate Alzheimer's disease pathology
    Authors: DC Shippy, C Wilhelm, PA Viharkumar, TJ Raife, TK Ulland
    Journal of Neuroinflammation, 2020-09-21;17(1):280.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Capture
  6. A Two-Cell Model for IL-1&beta Release Mediated by Death-Receptor Signaling
    Authors: CA Donado, AB Cao, DP Simmons, BA Croker, PJ Brennan, MB Brenner
    Cell Rep, 2020-04-07;31(1):107466.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Early growth response 1 deficiency protects host against Pseudomonas aeruginosa lung infection
    Authors: Z Pang, R Raudonis, C McCormick, Z Cheng
    Infect. Immun., 2019-12-17;0(0):.
    Species: Mouse
    Sample Types: BALF
    Applications: ELISA Detection
  8. Berberine ameliorates lipopolysaccharide?induced inflammatory responses in mouse inner medullary collecting duct?3 cells by downregulation of NF?kappaB pathway
    Authors: DG Kim, JW Choi, IJ Jo, MJ Kim, HS Lee, SH Hong, HJ Song, GS Bae, SJ Park
    Mol Med Rep, 2019-11-19;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  9. A20 prevents inflammasome-dependent arthritis by inhibiting macrophage necroptosis through its ZnF7 ubiquitin-binding domain
    Authors: A Polykratis, A Martens, RO Eren, Y Shirasaki, M Yamagishi, Y Yamaguchi, S Uemura, M Miura, B Holzmann, G Kollias, M Armaka, G van Loo, M Pasparakis
    Nat. Cell Biol., 2019-05-13;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Bioassay
  10. Obesity-associated inflammation promotes angiogenesis and breast cancer via angiopoietin-like 4
    Authors: R Kolb, P Kluz, ZW Tan, N Borcherdin, N Bormann, A Vishwakarm, L Balcziak, P Zhu, BS Davies, F Gourronc, LZ Liu, X Ge, BH Jiang, K Gibson-Cor, A Klingelhut, NS Tan, Y Zhu, FS Sutterwala, X Shen, W Zhang
    Oncogene, 2018-12-05;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ELISA Development (Detection)
  11. New mitochondrial DNA synthesis enables NLRP3 inflammasome activation
    Authors: Z Zhong, S Liang, E Sanchez-Lo, F He, S Shalapour, XJ Lin, J Wong, S Ding, E Seki, B Schnabl, AL Hevener, HB Greenberg, T Kisseleva, M Karin
    Nature, 2018-07-25;0(0):.
    Species: Mouse
    Sample Types: Serum
  12. Anti-inflammatory effects of a traditional Korean medicine: Ojayeonjonghwan
    Authors: SY Nam, KY Kim, MH Kim, JB Jang, SY Rah, HJ Chae, JM Lee, HM Kim, HJ Jeong
    Pharm Biol, 2017-12-01;55(1):1856-1862.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Detection)
  13. The calcineurin-NFAT axis contributes to host defense during Pseudomonas aeruginosa lung infection
    Authors: Z Pang, RD Junkins, AJ MacNeil, C McCormick, Z Cheng, WM Chen, TJ Lin
    J. Leukoc. Biol., 2017-10-10;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Development (Detection)
  14. Anti-inflammatory Effects of the Natural Compounds Cortex Phellodendri and Humulus japonicus on Pelvic Inflammatory Disease in Mice
    Authors: Y Oh, YS Kwon, BD Jung
    Int J Med Sci, 2017-07-18;14(8):729-734.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Development (Detection)
  15. Oryza sativa (Rice) Hull Extract Inhibits Lipopolysaccharide-Induced Inflammatory Response in RAW264.7 Macrophages by Suppressing Extracellular Signal-regulated Kinase, c-Jun N-terminal Kinase, and Nuclear Factor-?B Activation
    Authors: Yoonsook Kim
    Pharmacogn Mag, 2016-10-01;12(48):295-301.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Detection)
  16. AMCase is a crucial regulator of type 2 immune responses to inhaled house dust mites.
    Authors: Kim L, Morita R, Kobayashi Y, Eisenbarth S, Lee C, Elias J, Eynon E, Flavell R
    Proc Natl Acad Sci U S A, 2015-05-18;112(22):E2891-9.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Detection)
  17. Myeloid cell TRAF3 regulates immune responses and inhibits inflammation and tumor development in mice.
    Authors: Lalani A, Moore C, Luo C, Kreider B, Liu Y, Morse H, Xie P
    J Immunol, 2014-11-24;194(1):334-48.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Development
  18. Glimepiride reduces CD14 expression and cytokine secretion from macrophages.
    Authors: Ingham V, Williams A, Bate C
    J Neuroinflammation, 2014-06-21;11(0):115.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Detection)
  19. A tissue-specific role for Nlrp3 in tubular epithelial repair after renal ischemia/reperfusion.
    Authors: Bakker P, Butter L, Claessen N, Teske G, Sutterwala F, Florquin S, Leemans J
    Am J Pathol, 2014-05-11;184(7):2013-22.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  20. Phosphorylation of the adaptor ASC acts as a molecular switch that controls the formation of speck-like aggregates and inflammasome activity.
    Authors: Hara, Hideki, Tsuchiya, Kohsuke, Kawamura, Ikuo, Fang, Rendong, Hernandez-Cuellar, Eduardo, Shen, Yanna, Mizuguchi, Junichir, Schweighoffer, Edina, Tybulewicz, Victor, Mitsuyama, Masao
    Nat Immunol, 2013-11-03;14(12):1247-55.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  21. Regulator of calcineurin 1 suppresses inflammation during respiratory tract infections.
    Authors: Junkins R, MacNeil A, Wu Z, McCormick C, Lin T
    J Immunol, 2013-04-15;190(10):5178-86.
    Species: Mouse
    Sample Types: BALF
    Applications: ELISA Development (Detection)
  22. Adenosine is required for sustained inflammasome activation via the A(2)A receptor and the HIF-1alpha pathway.
    Authors: Ouyang X, Ghani A, Malik A, Wilder T, Colegio O, Flavell R, Cronstein B, Mehal W
    Nat Commun, 2013-01-01;4(0):2909.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  23. NLRP10 is a NOD-like receptor essential to initiate adaptive immunity by dendritic cells.
    Authors: Eisenbarth, Stephani, Williams, Adam, Colegio, Oscar R, Meng, Hailong, Strowig, Till, Rongvaux, Anthony, Henao-Mejia, Jorge, Thaiss, Christop, Joly, Sophie, Gonzalez, David G, Xu, Lan, Zenewicz, Lauren A, Haberman, Ann M, Elinav, Eran, Kleinstein, Steven H, Sutterwala, Fayyaz S, Flavell, Richard
    Nature, 2012-04-25;484(7395):510-3.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  24. Response patterns of cytokines/chemokines in two murine strains after irradiation.
    Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
    Cytokine, 2012-01-25;58(2):169-77.
    Species: Mouse
    Sample Types: Plasma
    Applications: Luminex Development
  25. Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin.
    Authors: Wu W, Mehta H, Chakrabarty K, Booth JL, Duggan ES, Patel KB, Ballard JD, Coggeshall KM, Metcalf JP
    J. Immunol., 2009-10-07;183(9):5799-806.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  26. TNF-alpha and IL-1beta mediate inflammatory hypernociception in mice triggered by B1 but not B2 kinin receptor.
    Authors: Cunha TM, Verri WA, Jr, Fukada SY, Guerrero AT, Santodomingo-Garzon T, Poole S, Parada CA, Ferreira SH, Cunha FQ
    Eur. J. Pharmacol., 2007-07-13;573(1):221-9.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Development
  27. Antiinflammatory effects of natural tetranortriterpenoids isolated from Carapa guianensis Aublet on zymosan-induced arthritis in mice.
    Authors: Penido C, Conte FP, Chagas MS, Rodrigues CA, Pereira JF, Henriques MG
    Inflamm. Res., 2006-11-01;55(11):457-64.
    Species: Mouse
    Sample Types: Synovial Fluid
    Applications: ELISA Development
  28. Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality.
    Authors: Osuchowski MF, Welch K, Siddiqui J, Remick DG
    J. Immunol., 2006-08-01;177(3):1967-74.
    Species: Mouse
    Sample Types: Plasma
    Applications: ELISA Development
  29. Sequential ELISA to profile multiple cytokines from small volumes.
    Authors: Osuchowski MF, Siddiqui J, Copeland S, Remick DG
    J. Immunol. Methods, 2005-07-01;302(1):172-81.
    Species: Mouse
    Sample Types: Plasma
    Applications: ELISA Development
  30. Selective macrophage suppression during sepsis.
    Authors: Ellaban E, Bolgos G, Remick D
    Cell. Immunol., 2005-02-26;231(1):103-11.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development

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Mouse IL-1 beta /IL-1F2 Biotinylated Antibody
By Anonymous on 11/21/2017
Application: ELISA Sample Tested: Serum and Plasma,Tissue Culture Media Species: Mouse

This antibody was paired with MAB4012 to create an ELISA to measure IL-1b in mouse samples.

ELISA Mouse IL-1 beta /IL-1F2 Biotinylated Antibody BAF401