Mouse IL-1 beta /IL-1F2 Antibody

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AF-401-NA
AF-401-SP
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Detection of Human and Mouse IL‑1 beta /IL‑1F2 by Western Blot.
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Mouse IL-1 beta /IL-1F2 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-1 beta /IL-1F2 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse IL-1 beta /IL-1F2
Val118-Ser269
Accession # NP_032387
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.25 µg/mL
See below
Simple Western
2.5 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize IL‑1 beta /IL‑1F2-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND50) is typically ≤0.25 µg/mL in the presence of 50 pg/mL Recombinant Mouse IL‑1 beta /IL‑1F2.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Mouse IL-1 beta /IL-1F2 antibody by Western Blot. View Larger

Detection of Human and Mouse IL‑1 beta /IL‑1F2 by Western Blot. Western blot shows lysates of THP-1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nM PMA for 24 hours and 10 µg/mL LPS for 4 hours and RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with 10 µg/mL LPS for 24 hours. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse IL-1 beta /IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-401-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for IL-1 beta /IL-1F2 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry IL-1 beta /IL-1F2 antibody in MCF-7 Human Cell Line by Immunocytochemistry (ICC). View Larger

IL‑1 beta /IL‑1F2 in MCF‑7 Human Cell Line. IL-1 beta /IL-1F2 was detected in immersion fixed MCF-7 human breast cancer cell line using Goat Anti-Mouse IL-1 beta /IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-401-NA) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry IL-1 beta /IL-1F2 antibody in Mouse Thymus by Immunohistochemistry (IHC-Fr). View Larger

IL‑1 beta /IL‑1F2 in Mouse Thymus. IL-1 beta /IL-1F2 was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse IL-1 beta /IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-401-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Simple Western Detection of Mouse IL-1 beta /IL-1F2 antibody by Simple Western TM. View Larger

Detection of Mouse IL‑1 beta /IL‑1F2 by Simple WesternTM. Simple Western lane view shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with 10 µg/mL LPS for 24 hours, loaded at 0.5 mg/mL. A specific band was detected for IL-1 beta /IL-1F2 at approximately 40 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Mouse IL-1 beta /IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-401-NA) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Neutralization Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutralization by Mouse IL-1 beta /IL-1F2 Antibody. View Larger

Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutralization by Mouse IL-1 beta /IL-1F2 Antibody. Recombinant Mouse IL-1 beta /IL-1F2 (401-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line) as measured by Resazurin (AR002). Proliferation elicited by Recombinant Mouse IL-1 beta /IL-1F2 (50 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-1 beta /IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-401-NA). The ND50 is typically ≤ 0.25 µg/mL.

Immunohistochemistry View Larger

Detection of IL‑1 beta /IL‑1F2 in Mouse Intestine. IL‑1 beta /IL‑1F2 was detected in perfusion fixed paraffin-embedded sections of Mouse Intestine using Goat Anti-Mouse IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-401-NA) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC006). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Western Blot Detection of Mouse IL-1 beta/IL-1F2 by Western Blot View Larger

Detection of Mouse IL-1 beta/IL-1F2 by Western Blot Quercetin inhibits auto-reactive NLRP3 inflammasome.(A) IL-1 beta concentrations in Nlrp3A350V/A350V and WT BMDM culture supernatants primed with LPS (500 ng/ml) for 90 min and treated with quercetin 1 h after LPS. (B) Supernatants of BMDM were analyzed by anti-ASC, anti-Caspase-1 immunoblotting. Lysates of BMDM were analyzed by anti-IL-1 beta and anti-beta -actin immunoblotting. Data shown are representative of two or more experiments (means ± SD) ***p < 0.001. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28148962), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse IL-1 beta/IL-1F2 by Western Blot View Larger

Detection of Mouse IL-1 beta/IL-1F2 by Western Blot GRA7-I-induced inflammasome activation was required for antimicrobial activity in MTB-infected macrophages.(A and B) BMDMs from PKC alpha +/+ and PKC alpha -/- mice (A) and BMDMs were transduced with lentivirus-shRNA-NS or lentivirus-shRNA-ASC for 2 days (B) infected with MTB (MOI = 1) for 4 h and then stimulated with rGRA7 (1, 5, 10 μg/ml) and its mutants for 18 h. IB analysis for IL-1 beta p17, IL-18 p18, or caspase-1 p10 in supernatants (SN), ASC, NLRP3, pro-IL-1 beta, pro-IL-18, or pro-caspase-1 in whole-cell lysates (WCL). Actin was used as a loading control. (C and D) Intracellular survival of MTB was assessed by CFU assay. BMDMs were infected with MTB for 4 h, followed by treatment with rGRA7, and then lysed to determine intracellular bacterial loads. The data are representative of five independent experiments with similar results (A and B). Data shown are the mean ± SD of five experiments (C and D). Significant differences (*P < 0.05; **P < 0.01; ***P < 0.001) compared with rVector. CFU, colony-forming units. ns, not significant. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.ppat.1006126), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse IL-1 beta/IL-1F2 by Western Blot View Larger

Detection of Mouse IL-1 beta/IL-1F2 by Western Blot M. bovis-induced inflammasome activation requires ERS. (A) Immunoblot analysis at different time-points of Bip and p-IRE1 alpha in lysates of BMDMs infected with M. bovis (MOI 10). (B) Immunoblot analysis at different time-points of IL-1 beta in supernatants of BMDMs infected with M. bovis (MOI 10). (C) ELISA for IL-1 beta analysis from supernatants of BMDMs infected with M. bovis (MOI 10). (D) IL-1 beta immunoblot analysis of supernatants from BMDM infected for 24 h with M. bovis (MOI 10) in the presence or absence of 4-PBA. (E) ELISA for IL-1 beta quantification from supernatants of BMDMs treated with or without 4-PBA for 1 h and then infected with M. bovis (MOI 10) for 24 h. ELISA for (F) TNF- alpha and (G) IL-6 detection in supernatants from M. bovis (MOI 10) infected BMDMs. ELISA for (H) TNF-alpha and (I) IL-6 detection from supernatants of BMDMs treated with or without 4-PBA for 1 h and then infected with M. bovis (MOI 10) for 24 h. TM, tunicamycin, positive control for ERS induced mitochondrial damage, 10 μg/mL; LPS+ATP, positive control for inflammasome activation, 200 ng/mL, and 1 mM, respectively; LPS, positive control for TNF-alpha and IL-6 production, 200 ng/mL; UNT, untreated; 4-PBA, 4-phenyl butyric acid, ERS inhibitor, 5 mM; MOI, multiplicity of infection. For (C,F,G), Data are representative of at least three independent experiments, each performed in triplicate for WB. The results are shown are the mean ± SD. The asterisks indicate statistically significant differences compared with untreated cells (*P < 0.05, **P < 0.01, ***P < 0.001, n.s., not significant). P-values were obtained by using one-way ANOVA followed by post-hoc Turkey' comparison test. For (E,H,I), data are representative of at least three independent experiments. The results are shown as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, n.s., not significant. P-values were analyzed using Student's t-test. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30846986), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Human IL-1 beta/IL-1F2 by Western Blot View Larger

Detection of Human IL-1 beta/IL-1F2 by Western Blot NLRP1A is activated by N-terminal proteolysis.(A) The amino acid sequence of the first 244 residues of NLRP1AB6 was aligned to NLRP1B129 and NLRP1BB6. The arrow above the alignment indicates the LF-cleavage site in NLRP1B129. (B) 293T cells were transfected for 36h with 200ng empty vector (V) or plasmids pcDNA3.1-HA-NLRP1B129 or -NLRP1AB6-MYC (CMV promoter) along with 200ng mCASP1 and 200ng mIL-1 beta expression vectors. Cells were treated overnight with anthrax lethal toxin (LeTx, 1μg/ml) 24h post-transfection, and then lysates were analyzed by immunoblotting (IB) with the indicated antibodies. (C) 293T cells were transfected for 36h with plasmids encoding 400ng GFP-HA-NLRP1B129 or GFP-HA-NLRP1AB6 (under the control of the LTR promoter in pMSCV) or with mutants engineered to contain an N-terminal TEV protease site. Cells were also co-transfected with 100ng empty vector (V) or plasmids encoding TEV-protease (pTEV) or lethal factor protease (pLF), plus 300ng empty pMSCV. (D) To assess IL-1 beta cleavage, cells were transfected as in C, but with 8ng of GFP-HA-NLRP1B and co-transfected with vectors encoding 200ng mCASP1 and 200ng mIL-1 beta. For panels B-D, data shown are representative of at least three similar experiments. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.ppat.1006052), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse IL-1 beta/IL-1F2 by Western Blot View Larger

Detection of Mouse IL-1 beta/IL-1F2 by Western Blot Colocalization of phagosome markers with internalized Bb in WT and MyD88−/− BMDMs. a-b Confocal 40x images of WT (a) and MyD88−/− (b) BMDMs after 6 h stimulation with Bb at MOI 10:1, depicting colocalization of Bb-containing phagosomes with LAMP-1. White box indicates phagosome depicted in inset. Graph shows the intensity of each indicated pixel across the white line (distance on x-axis). Green is Bb, red is LAMP-1 and yellow is actin. c Quantitation of colocalization between Bb and LAMP-1 in 10 phagosomes of WT (black dots) and MyD88−/− (red dots) BMDMs by measuring intensity difference between LAMP-1 staining and Bb staining. d Western blot of protein lysate isolated from WT BMDMs after 6 h stimulation with Bb +/− ATP (C = cell lysate, S = supernatant) Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/34000990), licensed under a CC-BY license. Not internally tested by R&D Systems.

In vivo assay Detection of Mouse IL-1 beta/IL-1F2 by In vivo assay View Larger

Detection of Mouse IL-1 beta/IL-1F2 by In vivo assay Differential effects of IL-1 beta neutralization on melanoma lung nodules in WT and DJ-1 KO mice.(A) IL-1 beta antibody (10μg) or control IgG was intravenously injected into mice once. The melanoma cells (6×104) were intravenously injected 30 minutes later. The mice were sacrificed three weeks later. Bar chart showed that the anti-IL-1 beta neutralizing antibody can effectively lower serum IL-1 beta levels as compared with control IgG in both WT and DJ-1 KO mice. Note that serum IL-1 beta levels were higher in DJ-1 KO mice than those in WT mice. (B) Inhibition of IL-1 beta neutralization on lung nodules formation in DJ-1 KO mice. Upper panels: melanoma nodules in lungs of WT (top) and KO (bottom) mice treated with control IgG (left) or neutralizing anti-IL-1 beta antibody (right). For each experimental condition, the gross and microscopic morphologies of tumor nodules are indicated by arrows, and are shown at left and right sites, respectively. Scale bars: 0.5 mm for lung photographs and 0.5 mm for H&E staining. Lower panel: Bar chart showed the summarized results of lung nodule numbers in WT and DJ-1 KO mice. Note that IL-1 beta neutralization enhanced and suppressed melanoma nodules formation in WT and DJ-1 KO mice, respectively. Data are presented as mean ± S.E.M. n = 5 for each group, * p < 0.05 compared with control IgG-treated WT mice, # p<0.05 compared with respective control IgG in WT and DJ-1 KO mice. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0115827), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse IL-1 beta/IL-1F2 by Western Blot View Larger

Detection of Mouse IL-1 beta/IL-1F2 by Western Blot Pro-IL-1 beta degradation is induced by the autophagy inhibitors chloroquine and bafilomycin A, and by the proteasome activator betulinic acid. (A) Chloroquine selectively attenuates IL-1 beta release from cardiac fibroblasts. The cells were incubated with LPS and/or 20 μM chloroquine phosphate as indicated for 18 h, then activated with 3 mM ATP for 60 min. IL-1 beta, TNF, MIP-2, and IL-6 were quantified in the conditioned media (n = 3). (B,C) Cardiac fibroblasts were incubated with LPS for 18 h with or without 20 μM chloroquine phosphate and pro-IL-1 beta quantified with western blot (n = 3). (D) Pro-IL-1 beta mRNA expression was quantified with qPCR (n = 3). (E,F) Cardiac fibroblasts were incubated with LPS for 20 h with or without 10% FCS or 20 μM chloroquine phosphate as indicated. The amount of total ubiquitinated protein was quantified with western blot (n = 4). (G,H) Cardiac fibroblasts were incubated with 10% FCS with or without 10 ng/mL LPS and/or 100 nM bafilomycin A1 for 20 h. Pro-IL-1 beta was quantified with western blot. (I,J) Cardiac fibroblasts were incubated with 10% FCS with or without 10 ng/mL LPS and/or 20 μM betulinic acid as indicated for 20 h. Pro-IL-1 beta was quantified with western blot. All columns are mean with SEM. *p < 0.05, **p < 0.01 (paired student t-test). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31244838), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Human IL-1 beta/IL-1F2 by Western Blot View Larger

Detection of Human IL-1 beta/IL-1F2 by Western Blot The B6 variant of NLRP1B is not cleaved by LF but can form an inflammasome in response to proteolysis.(A) The amino acid sequence of the first 244 residues of NLRP1BB6 was aligned to the homologous sequence of NLRP1B129. The arrow above the alignment indicates the LF-cleavage site in NLRP1B129. Asterisks indicate sites of amino acid identity. (B) For detection of NLRP1B expression, 293T cells were transfected with the indicated amounts of and empty vector (V) or plasmids encoding GFP-HA-NLRP1B129 or GFP-HA-NLRP1BB6 (construct schematics and the predicted molecular weight of each protein is depicted in the upper panel). Cells were treated overnight with anthrax lethal toxin protein (LeTx, 1μg/ml) 24h post-transfection, and then lysates were analyzed by immunoblotting (IB) with the indicated antibodies. For NLRP1 expression and cleavage, CASP1 and IL1B were omitted to prevent cell death and resulting apparent differences of expression. For blots probed with anti-HA (NLRP1B), the lysates were not boiled prior to loading to prevent aggregation and smearing of full-length and FIIND-processed NLRP1B on the immunoblot. To visualize the N-terminal processed form of NLRP1B, the lysates were boiled and resolved on a separate gel. (C) 293T Cells were transfected with the same amount of titrated NLRP1B encoding plasmid and treated as in B, but transfections also included plasmids encoding mouse CASP1 (200ng), IL-1 beta (200ng) and 200ng of empty vector. (D) For detection of NLRP1B expression, 293T cells were transfected for 36h with 250ng of plasmids encoding WT 129 or B6 NLRP1B or mutants engineered to express the TEV-protease site. Each plasmid was co-transfected with either 100ng of empty vector (V) or plasmids encoding TEV-protease (pTEV) or lethal factor protease (pLF), supplemented with 300ng empty vector (pMSCV). Cells were not treated with LeTx and lysates were analyzed by immunoblotting as in (B). (E) Cells were treated as in C, but with 8ng of plasmids encoding NLRP1B, along with 200ng of plasmids encoding mCASP-1 and mIL-1 beta, and 200ng empty vector to normalize plasmid quantities. Lower quantities of NLRP1B were transfected as compared to panel D so to avoid spontaneous NLPR1B activation. For panels B-E, data shown are representative of at least three similar experiments. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.ppat.1006052), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse IL-1 beta/IL-1F2 by Western Blot View Larger

Detection of Mouse IL-1 beta/IL-1F2 by Western Blot Pro-IL-1 beta degradation is induced by the autophagy inhibitors chloroquine and bafilomycin A, and by the proteasome activator betulinic acid. (A) Chloroquine selectively attenuates IL-1 beta release from cardiac fibroblasts. The cells were incubated with LPS and/or 20 μM chloroquine phosphate as indicated for 18 h, then activated with 3 mM ATP for 60 min. IL-1 beta, TNF, MIP-2, and IL-6 were quantified in the conditioned media (n = 3). (B,C) Cardiac fibroblasts were incubated with LPS for 18 h with or without 20 μM chloroquine phosphate and pro-IL-1 beta quantified with western blot (n = 3). (D) Pro-IL-1 beta mRNA expression was quantified with qPCR (n = 3). (E,F) Cardiac fibroblasts were incubated with LPS for 20 h with or without 10% FCS or 20 μM chloroquine phosphate as indicated. The amount of total ubiquitinated protein was quantified with western blot (n = 4). (G,H) Cardiac fibroblasts were incubated with 10% FCS with or without 10 ng/mL LPS and/or 100 nM bafilomycin A1 for 20 h. Pro-IL-1 beta was quantified with western blot. (I,J) Cardiac fibroblasts were incubated with 10% FCS with or without 10 ng/mL LPS and/or 20 μM betulinic acid as indicated for 20 h. Pro-IL-1 beta was quantified with western blot. All columns are mean with SEM. *p < 0.05, **p < 0.01 (paired student t-test). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31244838), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse IL-1 beta/IL-1F2 by Western Blot View Larger

Detection of Mouse IL-1 beta/IL-1F2 by Western Blot Rapamycin increases pro-IL-1 beta protein levels, rescues serum starvation-induced pro-IL-1 beta degradation, inhibits mTOR but induces no autophagy in LPS-stimulated cardiac fibroblasts. (A) IL-1 beta release was quantified in conditioned media from cardiac fibroblasts primed with 10 ng/mL LPS for 20 h with or without 500 nM rapamycin, as indicated, prior to activation with 3 mM ATP for 60 min. (B,C) Fibroblasts were primed with 10 ng/mL LPS for 20 h with or without 500 nM rapamycin and pro-IL-1 beta western blot performed (n = 3). (D,E) Control cells were incubated with 10% FCS, while serum deprived cells were incubated with or without 500 nM Rapamycin. Protein expressions were quantified with western blot (n = 4) (F,G). IL-1 beta and TNF-alpha were quantified in conditioned media from cardiac fibroblasts incubated with or without 10% FCS and 500 nM rapamycin and stimulated with LPS 10 ng/mL for 20 h prior to activation with 3 mM ATP for 60 min as indicated (n = 6) (H,I) IL-1 beta and TNF mRNA were quantified with PCR. (J–L) Cardiac fibroblasts were incubated with 10% FCS with or without 10 ng/mL LPS and/or 500 nM rapamycin for 20 h and mTOR (K) and p70 S6 kinase (L) activity were quantified with western blot (n = 5). (M,N) Cardiac fibroblasts primed with LPS (10 ng/mL) were incubated with 10% FCS (control) or no serum for 4 h. Cells were labeled with anti-LC3B and Hoechst and whole slides scanned with an automated immunofluorescence microscope. LC3B puncta in all cells in were automatically counted and ratio to number of Hoechst-labeled kernels calculated. Columns are mean with SEM of paired data normalized to control = 1 (n = 5). (O) Cardiac fibroblasts were incubated with 10% FCS, 10 ng/mL LPS, and/or 5 mM 3-methyladenine (3-MA) for 18 h and/or ATP 3 mM for 60 min as indicated (n = 3). Rapa: rapamycin. 3-MA: 3-methyladenine, ns: not significant, *p < 0.05, **p < 0.01, ****p < 0.0001 (paired student t-test). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31244838), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse IL-1 beta/IL-1F2 by Western Blot View Larger

Detection of Mouse IL-1 beta/IL-1F2 by Western Blot SHP deficiency increases caspase-1 cleavage and IL-1 beta maturation.(a–e) LPS-primed BMDMs from SHP+/+ and SHP−/− mice were stimulated with ATP (5 mM, a,d), nigericin (15 μM, b,e), or transfected with polyinosine–polycytidylic acid (poly I:C, 5 μg ml−1, c) for the indicated durations. (a–c) Supernatants were collected and subjected to ELISA for interleukin (IL)-1 beta, IL-18 and tumour necrosis factor (TNF)-alpha. (d,e) IB analysis for IL-1 beta p17 or caspase-1 p10 in supernatants (SN), SHP, pro-IL-1 beta or pro-caspase-1 in whole-cell lysates (WCL). Actin was used as a loading control. *P<0.05; **P<0.01; ***P<0.001, compared with SHP+/+ cell cultures (two-tailed Student’s t-test). Data are the means±s.d. of values from four independent experiments (a–c). Data are representative of three independent experiments with similar results (d,e). Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/ncomms7115), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse IL-1 beta/IL-1F2 by Western Blot View Larger

Detection of Mouse IL-1 beta/IL-1F2 by Western Blot ERS mediates M. bovis-induced inflammasome activation in vivo. (A) ELISA detection of IL-1 beta in serum samples from C57BL/6 mice treated with or without 4-PBA (18.6 mg/mouse/day) and infected with M. bovis (CFU 200) (n = 7). (B) Immunoblot analysis of NLRP3, AIM2, pro-IL-1 beta, and IL-1 beta in the lung tissues of M. bovis-infected mice in the presence or absence of 4-PBA. (C,D) Pathological lesions (H&E staining) in the lung of mice infected with M. bovis for 3 weeks (C) or 6 weeks (D) in the presence or absence of 4-PBA (18.6 mg/mouse/day) (n = 3). 4-PBA, 4-phenyl butyric acid, ERS inhibitor, 18.6 mg/mouse/day; CFU, colony forming units (n = 3). The results are shown as the mean ± SD. ***P < 0.001, n.s., not significant. P-values were analyzed by using Student's t-test (C,D). Scale bar = 100 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30846986), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse IL-1 beta/IL-1F2 by Western Blot View Larger

Detection of Mouse IL-1 beta/IL-1F2 by Western Blot POH1 negatively modifies K63-linked polyubiquitination of pro-IL-1 beta. a BMDMs stimulated with LPS for the indicated time periods were immunoprecipitated (IP) with an anti-POH1 or control IgG antibody and immunoblotted (IB) with the indicated antibodies. b HEK293T cells transfected with His-POH1 and either Flag-NLRP3, Flag-caspase-1 or Flag-ASC were subjected to IP with an anti-Flag antibody and IB with an anti-His antibody. c The lysates of HEK293T cells expressing Flag-POH1 and His-pro-IL-1 beta were IP with an anti-Flag antibody and IB with an anti-His antibody in cell lysates. d HEK293T cells transfected with Vsv-ASC, V5-tagged pro-caspase-1, HA-tagged K48-only or K63-only Ub and His-tagged pro-IL-1 beta, along with or without Flag-tagged POH1, were subjected to IP with an anti-V5 antibody or anti-His antibody and then IB with the indicated antibodies. ePoh1 delta /+ and Poh1 delta / delta BMDMs treated as indicated were subjected to IP with an anti-pro-IL-1 beta antibody and IB with the indicated antibodies. f HEK293T cells were transfected with Flag-caspase-1, Vsv-ASC, His-pro-IL-1 beta, Flag-POH1 (WT) or Flag-H113Q-POH1, along with HA-tagged K63-only Ub, then cells were subjected to IP with an anti-His or control IgG antibody and IB with the indicated antibodies. Similar results were obtained from three independent experiments Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30315153), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse IL-1 beta/IL-1F2 by Western Blot View Larger

Detection of Mouse IL-1 beta/IL-1F2 by Western Blot BTK inhibitors and its dysfunctional mutation suppress NLRP3 inflammasome activation.(a) Enzyme-linked immunosorbent assay (ELISA) of human IL-1 beta in supernatants and immunoblot analysis of human IL-1 beta p17, caspase-1 p20/p22 in supernatants and pro-IL-1 beta in cell lysates of THP-1-Mφs that were pretreated with the indicated inhibitors for 30 min and then stimulated with alum for 6 h. ELISA of murine IL-1 beta (b,c) and TNF-alpha (b) in supernatants of LPS-primed murine peritoneal macrophages that were pretreated with LFM-A13 and then stimulated with alum for 3 h. (d,e) Immunoblot analysis of the indicated proteins (d) and ELISA of murine IL-1 beta and IL-6 in supernatants of LPS-primed peritoneal macrophages from Xid and WT mice stimulated with alum for 6 h. (f) ELISA of murine IL-1 beta in supernatants of LPS-primed murine peritoneal macrophages pretreated with LFM-A13 and/or Syk inhibitor (R406), then stimulated with alum for 3 h. (g) ELISA of murine IL-1 beta and immunoblot analysis of murine caspase-1 p20 in supernatant of LPS-primed murine peritoneal macrophages stimulated with the indicated NLRP3 inflammasome activators for 3 h. Immunoblot analysis of murine IL-1 beta p17, caspase-1 p20 in supernatants, pro-IL-1 beta, pro-caspase-1 and ASC in cell lysates (h), and ELISA of murine IL-1 beta in supernatants (i) of LPS-primed murine peritoneal macrophages that were pretreated with LFM-A13 and then stimulated with alum or poly(dA:dT) for 3 h. Data are representative of three independent experiments. Data are presented as mean±s.d. (triplicate). **P<0.01; ***P<0.003. Two-sided Student's t-test. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26059659), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse IL-1 beta/IL-1F2 by Western Blot View Larger

Detection of Mouse IL-1 beta/IL-1F2 by Western Blot Rapamycin increases pro-IL-1 beta protein levels, rescues serum starvation-induced pro-IL-1 beta degradation, inhibits mTOR but induces no autophagy in LPS-stimulated cardiac fibroblasts. (A) IL-1 beta release was quantified in conditioned media from cardiac fibroblasts primed with 10 ng/mL LPS for 20 h with or without 500 nM rapamycin, as indicated, prior to activation with 3 mM ATP for 60 min. (B,C) Fibroblasts were primed with 10 ng/mL LPS for 20 h with or without 500 nM rapamycin and pro-IL-1 beta western blot performed (n = 3). (D,E) Control cells were incubated with 10% FCS, while serum deprived cells were incubated with or without 500 nM Rapamycin. Protein expressions were quantified with western blot (n = 4) (F,G). IL-1 beta and TNF-alpha were quantified in conditioned media from cardiac fibroblasts incubated with or without 10% FCS and 500 nM rapamycin and stimulated with LPS 10 ng/mL for 20 h prior to activation with 3 mM ATP for 60 min as indicated (n = 6) (H,I) IL-1 beta and TNF mRNA were quantified with PCR. (J–L) Cardiac fibroblasts were incubated with 10% FCS with or without 10 ng/mL LPS and/or 500 nM rapamycin for 20 h and mTOR (K) and p70 S6 kinase (L) activity were quantified with western blot (n = 5). (M,N) Cardiac fibroblasts primed with LPS (10 ng/mL) were incubated with 10% FCS (control) or no serum for 4 h. Cells were labeled with anti-LC3B and Hoechst and whole slides scanned with an automated immunofluorescence microscope. LC3B puncta in all cells in were automatically counted and ratio to number of Hoechst-labeled kernels calculated. Columns are mean with SEM of paired data normalized to control = 1 (n = 5). (O) Cardiac fibroblasts were incubated with 10% FCS, 10 ng/mL LPS, and/or 5 mM 3-methyladenine (3-MA) for 18 h and/or ATP 3 mM for 60 min as indicated (n = 3). Rapa: rapamycin. 3-MA: 3-methyladenine, ns: not significant, *p < 0.05, **p < 0.01, ****p < 0.0001 (paired student t-test). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31244838), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse IL-1 beta/IL-1F2 by Western Blot View Larger

Detection of Mouse IL-1 beta/IL-1F2 by Western Blot SHP inhibits the signal 2 activation of NLRP3 inflammasome.(a,b) LPS-primed BMDMs were treated with fenofibrate (10, 20, 50 μM for 4 h), or (c,d) LPS-primed THP-1 cells were treated with recombinant macrophage-stimulating protein (MSP 10, 50, 100 ng ml−1 for 4 h), and then activated with ATP (5 mM) for 30 min, followed by IB analysis of IL-1 beta p17 or caspase-1 p10 in supernatants (SN), pro-IL-1 beta or pro-caspase-1 in whole-cell lysates (WCL), with actin as a loading control. Data are representative of three independent experiments (a,c). Supernatants were collected and subjected to ELISA for IL-1 beta, IL-18, TNF-alpha and IL-8. Data are the means±s.d. of values from four independent experiments (b,d). *P<0.05; **P<0.01; ***P<0.001, compared with control (two-tailed Student’s t-test). FF, fenofibrate. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/ncomms7115), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse IL-1 beta/IL-1F2 by Western Blot View Larger

Detection of Mouse IL-1 beta/IL-1F2 by Western Blot The ESX-1 secretion system of Mtb does not affect secretion of proinflammatory cytokines in dendritic cells but is important for complete inflammasome activation.BMDCs were left uninfected (UI), infected with wild-type Mtb (Mtb) or the esxA deletion mutant ( delta esxA) for 4 h at MOI of 10, washed and incubated for an additional 24 h (A+B) or the indicated timepoints (C+D). (A) The cytokine profile in the supernatants was analyzed using a bead-based immunoassay (black =  uninfected, white =  Mtb, gray =  delta esxA). (B) and (C) IL-1 beta secretion was analyzed by ELISA. (D) The percent of cells with activated caspase-1 was determined via flow cytometry using fluorescent caspase-1 substrates (FLICA). (E) Pro-IL-1 beta protein levels in BMDCs. (F) GFP-labeled bacteria were used to infect BMDCs and rate of infection was determined via flow cytometry. Shown are means and standard deviation of triplicate measurements of one representative experiment out of three. In all figures, the asterisks denote range of p values (* = p<0.05, ** = 0.01>p>0.001,***p<0.001, ns =  not significant ) as determined by one way ANOVA with Tukey's post test. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0040722), licensed under a CC-BY license. Not internally tested by R&D Systems.

In vivo assay Detection of Mouse IL-1 beta/IL-1F2 by In vivo assay View Larger

Detection of Mouse IL-1 beta/IL-1F2 by In vivo assay Effects of IL-1 beta neutralization on the accumulation of MDSCs in lungs.(A) Immunofluorescent photos showed that Gr-1+ (green)/CD11b+ (red) cells indicate MDSCs and increased in lungs of DJ-1 KO mice. Treatment with anti-IL-1 beta neutralizing antibody antagonized the accumulation of MDSCs. DAPI was used to stain nuclei. (B) Bar chart showed the summarized results of MDSCs in lungs of WT and DJ-1 KO mice. Note that the neutralizing antibody significantly reduced the number of MDSCs accumulated in lungs. n = 9 for each group, * p < 0.05 compared with control IgG-treated WT mice, # p<0.05 compared with respective control IgG in WT and DJ-1 KO mice. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0115827), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse IL-1 beta/IL-1F2 by Western Blot View Larger

Detection of Mouse IL-1 beta/IL-1F2 by Western Blot Pro-IL-1 beta degradation is induced by the autophagy inhibitors chloroquine and bafilomycin A, and by the proteasome activator betulinic acid. (A) Chloroquine selectively attenuates IL-1 beta release from cardiac fibroblasts. The cells were incubated with LPS and/or 20 μM chloroquine phosphate as indicated for 18 h, then activated with 3 mM ATP for 60 min. IL-1 beta, TNF, MIP-2, and IL-6 were quantified in the conditioned media (n = 3). (B,C) Cardiac fibroblasts were incubated with LPS for 18 h with or without 20 μM chloroquine phosphate and pro-IL-1 beta quantified with western blot (n = 3). (D) Pro-IL-1 beta mRNA expression was quantified with qPCR (n = 3). (E,F) Cardiac fibroblasts were incubated with LPS for 20 h with or without 10% FCS or 20 μM chloroquine phosphate as indicated. The amount of total ubiquitinated protein was quantified with western blot (n = 4). (G,H) Cardiac fibroblasts were incubated with 10% FCS with or without 10 ng/mL LPS and/or 100 nM bafilomycin A1 for 20 h. Pro-IL-1 beta was quantified with western blot. (I,J) Cardiac fibroblasts were incubated with 10% FCS with or without 10 ng/mL LPS and/or 20 μM betulinic acid as indicated for 20 h. Pro-IL-1 beta was quantified with western blot. All columns are mean with SEM. *p < 0.05, **p < 0.01 (paired student t-test). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31244838), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse IL-1 beta/IL-1F2 by Western Blot View Larger

Detection of Mouse IL-1 beta/IL-1F2 by Western Blot Pro-IL-1 beta maturation is regulated by POH1-mediated deubiquitination. a, b HEK293T cells were transfected with plasmids encoding Flag-caspase-1, Vsv-ASC, His-pro-IL-1 beta (WT) or His-pro-IL-1 beta -K133R with different amounts (125, 250, 500 ng) of plasmid encoding Flag-POH1 and 500 ng of control plasmid, the secretion of a IL-1 beta in the supernatants was quantified by ELISA 36 h after transfection; b the cell lysates were IB with the indicated antibodies. c Mutants of murine pro-IL-1 beta with replacement of various lysine residues. d-f HEK293T cells were transfected with Flag-POH1, V5-caspase-1, V5-ASC, His-pro-IL-1 beta (WT) or its mutants, along d with or e-f without HA-tagged K63-only Ub, then d cells were subjected to IP with an anti-His antibody and IB with the indicated antibodies. e The IL-1 beta levels in supernatants were measured by ELISA; or f the cell lysates were collected and IB with the indicated antibodies. g Proposed function of POH1 as a negative regulator in mediating IL-1 beta processing. POH1, upregulated by TLR3/4 activation, interacts with and deubiquitinates pro-IL-1 beta, resulting in restriction of IL-1 beta cleavage and secretion. Similar results were obtained from three independent experiments. The results represent the mean ± s.d. of three independent sets of experiments. **p < 0.01, ***p < 0.001 (one-way ANOVA with Tukey’s post-hoc test) Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30315153), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse IL-1 beta/IL-1F2 by Western Blot View Larger

Detection of Mouse IL-1 beta/IL-1F2 by Western Blot Serum starvation selectively induces pro-IL-1 beta degradation in cardiac fibroblasts. (A) Cells were incubated with 10 ng/mL LPS for 5 or 20 h with or without 10% FCS and western blot analysis of pro-IL-1 beta and NLRP3 performed. Blots are representative of 3 independent experiments. (B) Protein bands were quantified and the ratios to beta -actin normalized to mean control = 1 (n = 3). (C,D) Cardiac fibroblasts were incubated with or without 10% FCS and primed with LPS for 20 h. Western blot analysis of complex II (mitochondrial mass marker) and NLRP3- inflammasome protein components (NEK7, NLRP3, ASC, pro-caspase-1, and pro-IL-1 beta ) were performed. Bands were quantified and the ratios to beta -actin normalized to mean control = 1 (n = 4, for ASC n = 10). (E,F) Cardiac fibroblasts (120,000 cells per well seeded in 6 well plates) were incubated with 10 ng/mL LPS for 20 h, with or without 10% FCS, then stained with 500 nM MitoTracker Deep Red for 45 min before MitoTracker fluorescence intensity was quantified with flow cytometry analysis (633 nm laser). All cells were analyzed and all gated cells included in the analysis. The presented dot plots (E) show the gate in red and are representative for 9 biological repeats. Median MitoTracker intensity with and without 10% FCS are shown (F). All columns are mean with SEM. *p < 0.05,**p < 0.01 (paired student t-test). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31244838), licensed under a CC-BY license. Not internally tested by R&D Systems.

In vivo assay Detection of Mouse IL-1 beta/IL-1F2 by In vivo assay View Larger

Detection of Mouse IL-1 beta/IL-1F2 by In vivo assay Differential effects of IL-1 beta neutralization on melanoma lung nodules in WT and DJ-1 KO mice.(A) IL-1 beta antibody (10μg) or control IgG was intravenously injected into mice once. The melanoma cells (6×104) were intravenously injected 30 minutes later. The mice were sacrificed three weeks later. Bar chart showed that the anti-IL-1 beta neutralizing antibody can effectively lower serum IL-1 beta levels as compared with control IgG in both WT and DJ-1 KO mice. Note that serum IL-1 beta levels were higher in DJ-1 KO mice than those in WT mice. (B) Inhibition of IL-1 beta neutralization on lung nodules formation in DJ-1 KO mice. Upper panels: melanoma nodules in lungs of WT (top) and KO (bottom) mice treated with control IgG (left) or neutralizing anti-IL-1 beta antibody (right). For each experimental condition, the gross and microscopic morphologies of tumor nodules are indicated by arrows, and are shown at left and right sites, respectively. Scale bars: 0.5 mm for lung photographs and 0.5 mm for H&E staining. Lower panel: Bar chart showed the summarized results of lung nodule numbers in WT and DJ-1 KO mice. Note that IL-1 beta neutralization enhanced and suppressed melanoma nodules formation in WT and DJ-1 KO mice, respectively. Data are presented as mean ± S.E.M. n = 5 for each group, * p < 0.05 compared with control IgG-treated WT mice, # p<0.05 compared with respective control IgG in WT and DJ-1 KO mice. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0115827), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse IL-1 beta/IL-1F2 by Western Blot View Larger

Detection of Mouse IL-1 beta/IL-1F2 by Western Blot Inflammasome assembly and mature IL-1 beta release in the spinal cord after sciatic nerve axotomy. a Speck-like costaining of NLRP3 and ASC in injured neurons 3 days after the injury (arrows). b Representative WB images of mature IL-1 beta and beta -actin proteins 3 days after unilateral axotomy of sciatic nerve. c Quantification of active IL-1 beta protein expression. N = 3 animals/group. The graph shows values normalized to beta -actin levels and relative to contralateral side. Mean values are shown on bars. Bars represent average ± SEM. **p < 0.01 (ANOVA with Bonferroni post hoc, ax. + vehicle vs. contralateral side). #p < 0.05, ##p < 0.01 (ANOVA with Bonferroni post hoc, compared to ax. + vehicle). Ax. axotomy Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35305649), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse IL-1 beta/IL-1F2 by Western Blot View Larger

Detection of Mouse IL-1 beta/IL-1F2 by Western Blot GRA7-I activated inflammasomes in an ASC-binding dependent manner.(A) Bacterially purified 6xHis-GRA7-I and its mutants were analyzed by Coomassie blue staining (left) or IB with alpha His (right). (B) BMDMs from PKC alpha +/+ and PKC alpha -/- (left) or BMDMs were transduced with lentivirus-shRNA-NS or lentivirus-shRNA-ASC (MOI = 100) with polybrene (8 μg/mL) (right) for 2 days, the cells were stimulated with rGRA7 (5 μg/ml) and its mutants for the indicated times and culture supernatants were harvested and analyzed for cytokine ELISA for IL-1 beta and IL-18. Data shown are the means ± SD of five experiments. Significant differences (*P < 0.05; ***P < 0.001) compared with PKC alpha +/+ or shRNA-NS. (C) IB analysis for IL-1 beta p17, IL-18 p18, or caspase-1 p10 in supernatants (SN), ASC, NLRP3, pro-IL-1 beta, pro-IL-18, or pro-caspase-1 in whole-cell lysates (WCL). Actin was used as a loading control. (D) IB analysis of lysates of BMDMs as in B and C solubilized with Triton X-100–containing buffer, followed by cross-linkage of insoluble fractions with disuccinimidyl suberate to capture ASC oligomers and analysis of those fractions (I + DSS) and soluble fractions (S) with antibody to ASC. Actin was used as a loading control. (E) Fluorescence confocal images showing formation of speck-like ASC pyroptosomes in BMDMs from PKC alpha +/+ and PKC alpha -/- mice were stimulated with rGRA7 and its mutants for 18 h, fixed, immunostained with antibodies for ASC (Alexa 488). Scale bar, 10 μm. The data are representative of five independent experiments with similar results (A and C-E). Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.ppat.1006126), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse IL-1 beta/IL-1F2 by Western Blot View Larger

Detection of Mouse IL-1 beta/IL-1F2 by Western Blot GRA7-I activated inflammasomes in an ASC-binding dependent manner.(A) Bacterially purified 6xHis-GRA7-I and its mutants were analyzed by Coomassie blue staining (left) or IB with alpha His (right). (B) BMDMs from PKC alpha +/+ and PKC alpha -/- (left) or BMDMs were transduced with lentivirus-shRNA-NS or lentivirus-shRNA-ASC (MOI = 100) with polybrene (8 μg/mL) (right) for 2 days, the cells were stimulated with rGRA7 (5 μg/ml) and its mutants for the indicated times and culture supernatants were harvested and analyzed for cytokine ELISA for IL-1 beta and IL-18. Data shown are the means ± SD of five experiments. Significant differences (*P < 0.05; ***P < 0.001) compared with PKC alpha +/+ or shRNA-NS. (C) IB analysis for IL-1 beta p17, IL-18 p18, or caspase-1 p10 in supernatants (SN), ASC, NLRP3, pro-IL-1 beta, pro-IL-18, or pro-caspase-1 in whole-cell lysates (WCL). Actin was used as a loading control. (D) IB analysis of lysates of BMDMs as in B and C solubilized with Triton X-100–containing buffer, followed by cross-linkage of insoluble fractions with disuccinimidyl suberate to capture ASC oligomers and analysis of those fractions (I + DSS) and soluble fractions (S) with antibody to ASC. Actin was used as a loading control. (E) Fluorescence confocal images showing formation of speck-like ASC pyroptosomes in BMDMs from PKC alpha +/+ and PKC alpha -/- mice were stimulated with rGRA7 and its mutants for 18 h, fixed, immunostained with antibodies for ASC (Alexa 488). Scale bar, 10 μm. The data are representative of five independent experiments with similar results (A and C-E). Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.ppat.1006126), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Human IL-1 beta/IL-1F2 by Western Blot View Larger

Detection of Human IL-1 beta/IL-1F2 by Western Blot The B6 variant of NLRP1B is not cleaved by LF but can form an inflammasome in response to proteolysis.(A) The amino acid sequence of the first 244 residues of NLRP1BB6 was aligned to the homologous sequence of NLRP1B129. The arrow above the alignment indicates the LF-cleavage site in NLRP1B129. Asterisks indicate sites of amino acid identity. (B) For detection of NLRP1B expression, 293T cells were transfected with the indicated amounts of and empty vector (V) or plasmids encoding GFP-HA-NLRP1B129 or GFP-HA-NLRP1BB6 (construct schematics and the predicted molecular weight of each protein is depicted in the upper panel). Cells were treated overnight with anthrax lethal toxin protein (LeTx, 1μg/ml) 24h post-transfection, and then lysates were analyzed by immunoblotting (IB) with the indicated antibodies. For NLRP1 expression and cleavage, CASP1 and IL1B were omitted to prevent cell death and resulting apparent differences of expression. For blots probed with anti-HA (NLRP1B), the lysates were not boiled prior to loading to prevent aggregation and smearing of full-length and FIIND-processed NLRP1B on the immunoblot. To visualize the N-terminal processed form of NLRP1B, the lysates were boiled and resolved on a separate gel. (C) 293T Cells were transfected with the same amount of titrated NLRP1B encoding plasmid and treated as in B, but transfections also included plasmids encoding mouse CASP1 (200ng), IL-1 beta (200ng) and 200ng of empty vector. (D) For detection of NLRP1B expression, 293T cells were transfected for 36h with 250ng of plasmids encoding WT 129 or B6 NLRP1B or mutants engineered to express the TEV-protease site. Each plasmid was co-transfected with either 100ng of empty vector (V) or plasmids encoding TEV-protease (pTEV) or lethal factor protease (pLF), supplemented with 300ng empty vector (pMSCV). Cells were not treated with LeTx and lysates were analyzed by immunoblotting as in (B). (E) Cells were treated as in C, but with 8ng of plasmids encoding NLRP1B, along with 200ng of plasmids encoding mCASP-1 and mIL-1 beta, and 200ng empty vector to normalize plasmid quantities. Lower quantities of NLRP1B were transfected as compared to panel D so to avoid spontaneous NLPR1B activation. For panels B-E, data shown are representative of at least three similar experiments. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.ppat.1006052), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse IL-1 beta/IL-1F2 by Western Blot View Larger

Detection of Mouse IL-1 beta/IL-1F2 by Western Blot SHP inhibits the signal 2 activation of NLRP3 inflammasome.(a,b) LPS-primed BMDMs were treated with fenofibrate (10, 20, 50 μM for 4 h), or (c,d) LPS-primed THP-1 cells were treated with recombinant macrophage-stimulating protein (MSP 10, 50, 100 ng ml−1 for 4 h), and then activated with ATP (5 mM) for 30 min, followed by IB analysis of IL-1 beta p17 or caspase-1 p10 in supernatants (SN), pro-IL-1 beta or pro-caspase-1 in whole-cell lysates (WCL), with actin as a loading control. Data are representative of three independent experiments (a,c). Supernatants were collected and subjected to ELISA for IL-1 beta, IL-18, TNF-alpha and IL-8. Data are the means±s.d. of values from four independent experiments (b,d). *P<0.05; **P<0.01; ***P<0.001, compared with control (two-tailed Student’s t-test). FF, fenofibrate. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/ncomms7115), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Human IL-1 beta/IL-1F2 by Western Blot View Larger

Detection of Human IL-1 beta/IL-1F2 by Western Blot NLRP1A is activated by N-terminal proteolysis.(A) The amino acid sequence of the first 244 residues of NLRP1AB6 was aligned to NLRP1B129 and NLRP1BB6. The arrow above the alignment indicates the LF-cleavage site in NLRP1B129. (B) 293T cells were transfected for 36h with 200ng empty vector (V) or plasmids pcDNA3.1-HA-NLRP1B129 or -NLRP1AB6-MYC (CMV promoter) along with 200ng mCASP1 and 200ng mIL-1 beta expression vectors. Cells were treated overnight with anthrax lethal toxin (LeTx, 1μg/ml) 24h post-transfection, and then lysates were analyzed by immunoblotting (IB) with the indicated antibodies. (C) 293T cells were transfected for 36h with plasmids encoding 400ng GFP-HA-NLRP1B129 or GFP-HA-NLRP1AB6 (under the control of the LTR promoter in pMSCV) or with mutants engineered to contain an N-terminal TEV protease site. Cells were also co-transfected with 100ng empty vector (V) or plasmids encoding TEV-protease (pTEV) or lethal factor protease (pLF), plus 300ng empty pMSCV. (D) To assess IL-1 beta cleavage, cells were transfected as in C, but with 8ng of GFP-HA-NLRP1B and co-transfected with vectors encoding 200ng mCASP1 and 200ng mIL-1 beta. For panels B-D, data shown are representative of at least three similar experiments. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.ppat.1006052), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse IL-1 beta/IL-1F2 by Western Blot View Larger

Detection of Mouse IL-1 beta/IL-1F2 by Western Blot POH1 inhibits inflammasome-induced pro-IL-1 beta processing. a-c BMDMs from Poh1 delta /+ and Poh1 delta / delta mice were primed with LPS for 12–14 h and then stimulated with ATP (0.5 h), nigericin (Nig, 0.5 h), poly(dA:dT) (5 h) or flagellin (5 h) as indicated, a IL-1 beta, b IL-6 and c TNF alpha levels in supernatants were analysed by ELISA. d-f BMDMs lentivirally transduced with control (Vector) or POH1 were stimulated as in a, d IL-1 beta, e IL-6 and f TNF alpha levels in supernatants were analysed by ELISA. g, h BMDMs g derived from Poh1 delta /+ and Poh1 delta / delta mice or h infected by indicated lentiviruses were treated as in a, the cell lysates (CL) and supernatants (SN) were collected and immunoblotted with the indicated antibodies. Similar results were obtained from three independent experiments. The results represent the mean ± s.d. of three independent sets of experiments. **p < 0.01, ***p < 0.001 (two-tailed Student’s t-test) Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30315153), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse IL-1 beta/IL-1F2 by Western Blot View Larger

Detection of Mouse IL-1 beta/IL-1F2 by Western Blot Quercetin inhibits NLRP3 and AIM2 Inflammasomes.LPS (500 ng/ml)-primed BMDM were treated with the indicated flavonoids and concentrations or vehicle (DMSO 0.01%), then stimulated with (A) 5 mM ATP (B) 10 μM Nigericin (C) 130 μg/ml Alum or (D) 400 ng/ml Poly (dA:dT) 30 minutes after treatment. (A–D) IL-1 beta and (E) TNF-alpha concentration in the culture supernatant were measured by ELISA. (B,F,G) LPS-primed BMDM were stimulated with Nigericin treated with quercetin 30 minutes before secondary stimulation. Supernatants and Lysate of BMDM were analyzed by immunoblotting. Cells were stained with FLICA and Caspase-1 activation was determined by FLICA positivity. Data shown are representative of two or more independent experiments (means ± SD) *p < 0.05, **p < 0.01, ***p < 0.001. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28148962), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse IL-1 beta/IL-1F2 by Western Blot View Larger

Detection of Mouse IL-1 beta/IL-1F2 by Western Blot GRA7-I-induced inflammasome activation was required for antimicrobial activity in MTB-infected macrophages.(A and B) BMDMs from PKC alpha +/+ and PKC alpha -/- mice (A) and BMDMs were transduced with lentivirus-shRNA-NS or lentivirus-shRNA-ASC for 2 days (B) infected with MTB (MOI = 1) for 4 h and then stimulated with rGRA7 (1, 5, 10 μg/ml) and its mutants for 18 h. IB analysis for IL-1 beta p17, IL-18 p18, or caspase-1 p10 in supernatants (SN), ASC, NLRP3, pro-IL-1 beta, pro-IL-18, or pro-caspase-1 in whole-cell lysates (WCL). Actin was used as a loading control. (C and D) Intracellular survival of MTB was assessed by CFU assay. BMDMs were infected with MTB for 4 h, followed by treatment with rGRA7, and then lysed to determine intracellular bacterial loads. The data are representative of five independent experiments with similar results (A and B). Data shown are the mean ± SD of five experiments (C and D). Significant differences (*P < 0.05; **P < 0.01; ***P < 0.001) compared with rVector. CFU, colony-forming units. ns, not significant. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.ppat.1006126), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Human IL-1 beta/IL-1F2 by Western Blot View Larger

Detection of Human IL-1 beta/IL-1F2 by Western Blot P. gingivalis and its OMVs differentially induce inflammasome signaling and pyroptosis in human macrophages. MDM were infected as before (2 h at MOI of 25:1, see Materials and Methods) with viable P. gingivalis (Pg), heat-killed-Pg (HK-Pg), OMVs, or heat-inactivated-OMVs (HI-OMVs) and (A) the activation of inflammasome components in the lysates was measured after 24 h by Western blot; beta -actin serves as a loading control throughout. Western blot data are representative of at least three independent experiments. (B) Production of IL-1 beta and IL-18 (by ELISA) from MDM was measured in the supernatant at 24 h. Data are mean ± SD from four independent experiments. Cell viability was determined by measuring extracellular LDH release and by 7-AAD exclusion (by flow-cytometry) at 24 h. 7-AAD is a membrane impermeant dye that is excluded from viable cells. The percent of 7-AAD positive (±SD) cells from three independent experiments was calculated relative to the untreated control. MDM treated with H2O2 (1 mM for 60 min) were included as positive control for 7-AAD. A representative histogram is shown. Cells were also treated where indicated with OMVs in the presence of KYT-1 and KYT-36 (10 μM of each). *p < 0.05: n.s, not significant. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28824884), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse IL-1 beta/IL-1F2 by Western Blot View Larger

Detection of Mouse IL-1 beta/IL-1F2 by Western Blot Pro-IL-1 beta degradation is induced by the autophagy inhibitors chloroquine and bafilomycin A, and by the proteasome activator betulinic acid. (A) Chloroquine selectively attenuates IL-1 beta release from cardiac fibroblasts. The cells were incubated with LPS and/or 20 μM chloroquine phosphate as indicated for 18 h, then activated with 3 mM ATP for 60 min. IL-1 beta, TNF, MIP-2, and IL-6 were quantified in the conditioned media (n = 3). (B,C) Cardiac fibroblasts were incubated with LPS for 18 h with or without 20 μM chloroquine phosphate and pro-IL-1 beta quantified with western blot (n = 3). (D) Pro-IL-1 beta mRNA expression was quantified with qPCR (n = 3). (E,F) Cardiac fibroblasts were incubated with LPS for 20 h with or without 10% FCS or 20 μM chloroquine phosphate as indicated. The amount of total ubiquitinated protein was quantified with western blot (n = 4). (G,H) Cardiac fibroblasts were incubated with 10% FCS with or without 10 ng/mL LPS and/or 100 nM bafilomycin A1 for 20 h. Pro-IL-1 beta was quantified with western blot. (I,J) Cardiac fibroblasts were incubated with 10% FCS with or without 10 ng/mL LPS and/or 20 μM betulinic acid as indicated for 20 h. Pro-IL-1 beta was quantified with western blot. All columns are mean with SEM. *p < 0.05, **p < 0.01 (paired student t-test). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31244838), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse IL-1 beta/IL-1F2 by Western Blot View Larger

Detection of Mouse IL-1 beta/IL-1F2 by Western Blot P. gingivalis and its OMVs differentially induce inflammasome signaling and pyroptosis in murine macrophages. BMM were infected as before (2 h at MOI of 25:1, see Materials and Methods) with viable P. gingivalis (Pg), heat-killed-Pg (HK-Pg), OMVs, or heat-inactivated-OMVs (HI-OMVs) and (A) the activation of inflammasome components in the lysates [or supernatants (sup) where indicated] measured after 24 h by Western blot; beta -actin serves as a loading control throughout. Western blot data are representative of at least three independent experiments. (B) Production of IL-1 beta and IL-18 (by ELISA) from BMM was measured in the supernatant at 24 h. Data are mean ± SD from four independent experiments. Cell viability was determined by measuring extracellular LDH release and by 7-AAD exclusion (by flow-cytometry) at 24 h. 7-AAD is a membrane impermeant dye that is excluded from viable cells. The percent of 7-AAD positive (±SD) cells from three independent experiments was calculated relative to the untreated control. BMM treated with H2O2 (1 mM for 60 min) were included as positive control for 7-AAD. A representative histogram is shown. Cells were also treated where indicated with OMVs in the presence of KYT-1 and KYT-36 (10 μM of each). *p < 0.05. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28824884), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse IL-1 beta/IL-1F2 by Western Blot View Larger

Detection of Mouse IL-1 beta/IL-1F2 by Western Blot Caspase-1 but not gasdermin-D processing depends on ASC oligomerization.(a) Western blot analysis for cleaved caspase-1 p20, IL-1 beta p17, and HMGB-1 in cell supernatants (SN) and pro-caspase-1, pro-IL-1 beta and HMGB-1 in cell lysates (lys) of LPS-primed immortalized Asc−/− BMDMs or Asc−/− BMDMs expressing ASCFL, ASCY59A or ASCE80R 3 h after poly(dA:dT) transfection (1 μg ml−1). (b) Release of LDH from LPS-primed immortalized Asc−/− BMDMs or Asc−/− BMDMs expressing ASCFL, ASCY59A or ASCE80R, or derived Casp1 knockouts 3 h after poly(dA:dT) transfection (1 μg ml−1). (c) Western blot analysis for processing of full-length gasdermin-D (GSDMDFL) into the active N-terminal fragment (GSDMDN-term) in combined lysates and supernatants (lys + SN) of LPS-primed immortalized Asc−/− BMDMs expressing ASCFL, ASCY59A or ASCE80R, or derived Casp1 knockouts 3 h after poly(dA:dT) transfection (1 μg ml−1). Arrowhead, gasdermin-DNterm p30; *a cross-reacting band. (d) Release of LDH from LPS-primed primary C57BL/6 WT (WT), Casp1−/−/Casp11−/− or Asc−/− BMDMs infected with S. Typhimurium (multiplicity of infection (MOI)=10, 1 h). (e) Western blot analysis for processing of full-length gasdermin-D (GSDMDFL) into the active N-terminal fragment (GSDMDN-term) in combined lysates and supernatants (lys+SN) of LPS-primed primary C57BL/6 WT (WT), Casp1−/−/Casp11−/− or Asc−/− BMDMs infected with S. Typhimurium (MOI=10, 1 h) or left uninfected. Arrowhead, gasdermin-DNterm p30; *a cross-reacting band; ** a S. Typhimurium-specific cross-reactive band. See also Supplementary Figs 8 and 9. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/ncomms11929), licensed under a CC-BY license. Not internally tested by R&D Systems.

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Background: IL-1 beta/IL-1F2

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2, IL1B), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 17% amino acid (aa) identity in mouse. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. The 17 kDa molecular weight mature mouse IL-1 beta shares 90% aa sequence identity with cotton rat and rat and 67%-78% with canine, equine, feline, human, porcine, and rhesus macaque IL-1 beta. IL-1 beta functions in a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases.

Long Name
Interleukin 1 beta
Entrez Gene IDs
3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
Alternate Names
catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta

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Citations for Mouse IL-1 beta /IL-1F2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

273 Citations: Showing 1 - 10
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  1. The arginase 1/ornithine decarboxylase pathway suppresses HDAC3 to ameliorate the myeloid cell inflammatory response: implications for retinal ischemic injury
    Authors: Shosha, E;Shahror, RA;Morris, CA;Xu, Z;Lucas, R;McGee-Lawrence, ME;Rusch, NJ;Caldwell, RB;Fouda, AY;
    Cell death & disease
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  2. The Inflammasome Pathway is Activated by Dengue Virus Non-structural Protein 1 and is Protective During Dengue Virus Infection
    Authors: Wong, MP;Juan, EYW;Chelluri, SS;Wang, P;Pahmeier, F;Castillo-Rojas, B;Blanc, SF;Biering, SB;Vance, RE;Harris, E;
    bioRxiv : the preprint server for biology
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Suppressing high mobility group box-1 release alleviates morphine tolerance via the adenosine 5'-monophosphate-activated protein kinase/heme oxygenase-1 pathway
    Authors: TT Lin, CY Jiang, L Sheng, L Wan, W Fan, JC Li, XD Sun, CJ Xu, L Hu, XF Wu, Y Han, WT Liu, YB Pan
    Neural regeneration research, 2023-09-01;18(9):2067-2074.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Inhibition of STAT-mediated cytokine responses to chemically-induced colitis prevents inflammation-associated neurobehavioral impairments
    Authors: Sin, R;Sotogaku, N;Ohnishi, YN;Shuto, T;Kuroiwa, M;Kawahara, Y;Sugiyama, K;Murakami, Y;Kanai, M;Funakoshi, H;Chakraborti, A;Bibb, JA;Nishi, A;
    Brain, behavior, and immunity
    Species: Mouse
    Sample Types: Cell Lysates, Tissue Homogenates
    Applications: Western Blot
  5. Disulfiram blocks inflammatory TLR4 signaling by targeting MD-2
    Authors: Bai, Y;Min, R;Chen, P;Mei, S;Deng, F;Zheng, Z;Jiang, C;Miao, R;Wu, Z;Zhang, P;Pan, Y;Lieberman, J;Liu, X;
    Proceedings of the National Academy of Sciences of the United States of America
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Parthenolide targets NLRP3 to treat inflammasome-related diseases
    Authors: Liu, L;Feng, L;Gao, J;Hu, J;Li, A;Zhu, Y;Zhang, C;Qiu, B;Shen, Z;
    International immunopharmacology
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Bioassay
  7. Candida auris uses metabolic strategies to escape and kill macrophages while avoiding robust activation of the NLRP3 inflammasome response
    Authors: Weerasinghe, H;Simm, C;Djajawi, TM;Tedja, I;Lo, TL;Simpson, DS;Shasha, D;Mizrahi, N;Olivier, FAB;Speir, M;Lawlor, KE;Ben-Ami, R;Traven, A;
    Cell reports
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Pirfenidone Inhibits Alveolar Bone Loss in Ligature-Induced Periodontitis by Suppressing the NF-?B Signaling Pathway in Mice
    Authors: Zhang, Z;Song, J;Kwon, SH;Wang, Z;Park, SG;Piao, X;Ryu, JH;Kim, N;Kim, OS;Kim, SH;Koh, JT;
    International journal of molecular sciences
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. Ergolide covalently binds NLRP3 and inhibits NLRP3 inflammasome-mediated pyroptosis
    Authors: Ren, M;Chen, J;Xu, H;Li, W;Wang, T;Chi, Z;Lin, Y;Zhang, A;Chen, G;Wang, X;Sun, X;Liang, G;Wang, J;Luo, W;
    International immunopharmacology
    Species: Transgenic Mouse, Mouse
    Sample Types: Cell Culture Supernates, Cell Lysates
    Applications: Western Blot
  10. Endosomal trafficking of two-pore K+ efflux channel TWIK2 to plasmalemma mediates NLRP3 inflammasome activation and inflammatory injury
    Authors: Huang, LS;Anas, M;Xu, J;Zhou, B;Toth, PT;Krishnan, Y;Di, A;Malik, AB;
    eLife
    Species: Mouse
    Sample Types: Cell Culture Supernates
  11. Gut microbiota changes require vagus nerve integrity to promote depressive-like behaviors in mice
    Authors: Siopi, E;Galerne, M;Rivagorda, M;Saha, S;Moigneu, C;Moriceau, S;Bigot, M;Oury, F;Lledo, PM;
    Molecular psychiatry
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  12. A bacterial autotransporter impairs innate immune responses by targeting the transcription factor TFE3
    Authors: A Ta, R Ricci-Azev, SO Vasudevan, SS Wright, P Kumari, MS Havira, M Surendran, VA Rathinam, SK Vanaja
    Nature Communications, 2023-04-11;14(1):2035.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  13. Phosphate induces inflammation and exacerbates injury from cigarette smoke in the bronchial epithelium
    Authors: S Bollenbeck, K Heitman, B Czaya, M Easter, MJ Hirsch, S Vang, E Harris, ES Helton, JW Barnes, C Faul, S Krick
    Scientific Reports, 2023-03-25;13(1):4898.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  14. Novel Activity of ODZ10117, a STAT3 Inhibitor, for Regulation of NLRP3 Inflammasome Activation
    Authors: JH Kang, SB Lee, J Seok, DH Kim, G Ma, J Park, AJ Jeong, SK Ye, TB Kang
    International Journal of Molecular Sciences, 2023-03-23;24(7):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  15. HIF1alpha-BNIP3-mediated mitophagy protects against renal fibrosis by decreasing ROS and inhibiting activation of the NLRP3 inflammasome
    Authors: J Li, Q Lin, X Shao, S Li, X Zhu, J Wu, S Mou, L Gu, Q Wang, M Zhang, K Zhang, J Lu, Z Ni
    Cell Death & Disease, 2023-03-17;14(3):200.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Cells, Whole Tissue
    Applications: ICC, IHC, Western Blot
  16. Macrophage fumarate hydratase restrains mtRNA-mediated interferon production
    Authors: A Hooftman, CG Peace, DG Ryan, EA Day, M Yang, AF McGettrick, M Yin, EN Montano, L Huo, JE Toller-Kaw, V Zecchini, TAJ Ryan, A Bolado-Car, AM Casey, HA Prag, ASH Costa, G De Los San, M Ishimori, DJ Wallace, S Venuturupa, E Nikitopoul, N Frizzell, C Johansson, A Von Kriegs, MP Murphy, C Jefferies, C Frezza, LAJ O'Neill
    Nature, 2023-03-08;615(7952):490-498.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  17. TREM-1 triggers necroptosis of macrophages through mTOR-dependent mitochondrial fission during acute lung injury
    Authors: WJ Zhong, J Zhang, JX Duan, CY Zhang, SC Ma, YS Li, NS Yang, HH Yang, JB Xiong, CX Guan, ZX Jiang, ZJ You, Y Zhou
    Journal of Translational Medicine, 2023-03-06;21(1):179.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  18. Aim2 Deficiency Ameliorates Lacrimal Gland Destruction and Corneal Epithelium Defects in an Experimental Dry Eye Model
    Authors: Y Chen, J Pu, X Li, L Lian, C Ge, Z Liu, W Wang, L Hou, W Chen, J Li
    Investigative Ophthalmology & Visual Science, 2023-03-01;64(3):26.
    Species: Mouse
    Sample Types: Protein Lysates
    Applications: Western Blot
  19. Regulatory T cells alleviate myelin loss and cognitive dysfunction by regulating neuroinflammation and microglial pyroptosis via TLR4/MyD88/NF-kappaB pathway in LPC-induced demyelination
    Authors: Y Wang, D Sadike, B Huang, P Li, Q Wu, N Jiang, Y Fang, G Song, L Xu, W Wang, M Xie
    Journal of Neuroinflammation, 2023-02-18;20(1):41.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  20. Negative regulation of TREM2-mediated C9orf72 poly-GA clearance by the NLRP3 inflammasome
    Authors: X Shu, C Wei, WY Tu, K Zhong, S Qi, A Wang, L Bai, SX Zhang, B Luo, ZZ Xu, K Zhang, C Shen
    Cell Reports, 2023-02-16;42(2):112133.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  21. LRRK2 Kinase Inhibition Attenuates Astrocytic Activation in Response to Amyloid beta1-42 Fibrils
    Authors: A Filippini, V Salvi, V Dattilo, C Magri, S Castrezzat, R Veerhuis, D Bosisio, M Gennarelli, I Russo
    Biomolecules, 2023-02-06;13(2):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  22. Cardiolipin coordinates inflammatory metabolic reprogramming through regulation of Complex II disassembly and degradation
    Authors: MB Reynolds, HS Hong, BC Michmerhui, AE Lawrence, L Zhang, JS Knight, CA Lyssiotis, BH Abuaita, MX O'Riordan
    Science Advances, 2023-02-03;9(5):eade8701.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  23. Carnosic acid inhibits NLRP3 inflammasome activation by targeting both priming and assembly steps
    Authors: G Lin, N Li, D Li, L Chen, H Deng, S Wang, J Tang, W Ouyang
    International immunopharmacology, 2023-02-02;116(0):109819.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  24. The NLRP3 Inflammasome Is Required for Protection Against Pseudomonas Keratitis
    Authors: A Ramadan, Z Cao, M Gadjeva, TS Zaidi, VA Rathinam, N Panjwani
    Investigative Ophthalmology & Visual Science, 2023-02-01;64(2):11.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  25. Phenolic and quinone methide nor-triterpenes as selective NLRP3 inflammasome inhibitors
    Authors: L González-C, J P Green, I Cuadrado, Á Amesty, S Oramas-Roy, David Brou, A Estévez-Br, S Hortelano, B de Las Her
    Bioorganic chemistry, 2023-01-14;132(0):106362.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  26. Tabersonine, a natural NLRP3 inhibitor, suppresses inflammasome activation in macrophages and attenuate NLRP3-driven diseases in mice
    Authors: HW Xu, WF Li, SS Hong, JJ Shao, JH Chen, N Chattipako, D Wu, W Luo, G Liang
    Acta pharmacologica Sinica, 2023-01-10;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  27. JC2-11, a benzylideneacetophenone derivative, attenuates inflammasome activation
    Authors: G Lee, H Ahn, JH Yun, J Park, E Lee, S Oh, GS Lee
    Scientific Reports, 2022-12-28;12(1):22484.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  28. Glucosidase inhibitor, Nimbidiol ameliorates renal fibrosis and dysfunction in type-1 diabetes
    Authors: SK Juin, S Pushpakuma, SC Tyagi, U Sen
    Scientific Reports, 2022-12-15;12(1):21707.
    Species: Mouse
    Sample Types: Tissue Lysates
    Applications: Western Blot
  29. Lysosomal cathepsins act in concert with Gasdermin-D during NAIP/NLRC4-dependent IL-1beta secretion
    Authors: LM Branco, MP Amaral, H Boekhoff, ABF de Lima, IS Farias, SL Lage, GJS Pereira, BS Franklin, KR Bortoluci
    Cell Death & Disease, 2022-12-08;13(12):1029.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  30. Neutrophil inflammasomes sense the subcellular delivery route of translocated bacterial effectors and toxins
    Authors: C Oh, L Li, A Verma, AD Reuven, EA Miao, JB Bliska, Y Aachoui
    Cell Reports, 2022-11-22;41(8):111688.
    Species: Mouse, Transgenic Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  31. Caspase-8 inactivation drives autophagy-dependent inflammasome activation in myeloid cells
    Authors: YH Wu, ST Mo, IT Chen, FY Hsieh, SL Hsieh, J Zhang, MZ Lai
    Science Advances, 2022-11-11;8(45):eabn9912.
    Species: Transgenic Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  32. Transcriptional licensing is required for Pyrin inflammasome activation in human macrophages and bypassed by mutations causing familial Mediterranean fever
    Authors: MSJ Mangan, F Gorki, K Krause, A Heinz, A Pankow, T Ebert, D Jahn, K Hiller, V Hornung, M Maurer, FI Schmidt, R Gerhard, E Latz
    PloS Biology, 2022-11-07;20(11):e3001351.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  33. Lipocalin 2 activates the NLRP3 inflammasome via LPS?induced NF?kappaB signaling and plays a role as a pro?inflammatory regulator in murine macrophages
    Authors: SL Kim, MW Shin, SW Kim
    Molecular Medicine Reports, 2022-10-20;26(6):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  34. HDAC5 RNA interference ameliorates acute renal injury by upregulating KLF2 and inhibiting NALP3 expression in a mouse model of oxalate nephropathy
    Authors: P Sharma, K Karnam, A Mahale, K Sedmaki, V Krishna Ve, OP Kulkarni
    International immunopharmacology, 2022-09-29;112(0):109264.
    Species: Mouse
    Sample Types: Tissue Homoegenates
    Applications: Western Blot
  35. PD-1/PD-L1 blockade abrogates a dysfunctional innate-adaptive immune axis in critical beta-coronavirus disease
    Authors: M Duhalde Ve, D Olivera, G Gastão Dav, M Bertullo, V Noya, G Fabiano de, S Primon Mur, I Castro, AP Arévalo, M Crispo, G Galliussi, S Russo, D Charbonnie, F Rammauro, M Jeldres, C Alamón, V Varela, C Batthyany, M Bollati-Fo, P Oppezzo, O Pritsch, JL Proença-Mó, HI Nakaya, E Trias, L Barbeito, I Anegon, MC Cuturi, P Moraes-Vie, M Segovia, M Hill
    Science Advances, 2022-09-21;8(38):eabn6545.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  36. Systemic Administration of Pegylated Arginase-1 Attenuates the Progression of Diabetic Retinopathy
    Authors: AA Abdelrahma, KL Bunch, PV Sandow, PN Cheng, RB Caldwell, RW Caldwell
    Cells, 2022-09-16;11(18):.
    Species: Mouse
    Sample Types: Protein Lysates
    Applications: Western Blot
  37. Neutrophil-mediated fibroblast-tumor cell il-6/stat-3 signaling underlies the association between neutrophil-to-lymphocyte ratio dynamics and chemotherapy response in localized pancreatic cancer: A hybrid clinical-preclinical study
    Authors: I de Castro, A Bianchi, NU Deshpande, P Sharma, S Mehra, VT Garrido, SJ Saigh, J England, PJ Hosein, D Kwon, NB Merchant, J Datta
    Elife, 2022-09-15;11(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  38. FAAH served a key membrane-anchoring and stabilizing role for NLRP3 protein independently of the endocannabinoid system
    Authors: Y Zhu, H Zhang, H Mao, S Zhong, Y Huang, S Chen, K Yan, Z Zhao, X Hao, Y Zhang, H Yao, X Huang, M Wang, W Zhang, J Li, G Meng, X Qin, Z Ye, J Shen, Y Song, Y Xu, Z Yang, L Wang, Y Zhang, L Wen
    Cell Death and Differentiation, 2022-09-14;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  39. Identification of lncRNA DLEU2 as a potential diagnostic biomarker and anti-inflammatory target for ulcerative colitis
    Authors: Q Lin, D Zhang, J Zhang, W Luo, Z Xu, J Yao, L Wang
    Frontiers in Pharmacology, 2022-09-14;13(0):991448.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  40. Cross-talk between IL-6 trans-signaling and AIM2 inflammasome/IL-1beta axes bridge innate immunity and epithelial apoptosis to promote emphysema
    Authors: SM Ruwanpura, L McLeod, LF Dousha, HJ Seow, AC West, AJ West, T Weng, M Alanazi, M MacDonald, PT King, PG Bardin, C Gabay, DM Klinman, S Bozinovski, R Vlahos, GP Anderson, S Rose-John, MI Saad, BJ Jenkins
    Proceedings of the National Academy of Sciences of the United States of America, 2022-08-29;119(36):e2201494119.
    Species: Mouse
    Sample Types: Serum
    Applications: Western Blot
  41. An injectable thermosensitive hydrogel for dual delivery of diclofenac and Avastin� to effectively suppress inflammatory corneal neovascularization
    Authors: H Shi, Y Zhu, C Xing, S Li, Z Bao, L Lei, D Lin, Y Wang, H Chen, X Xu
    International journal of pharmaceutics, 2022-08-05;0(0):122081.
    Species: Rabbit
    Sample Types: Whole Tissue
    Applications: IHC
  42. Pramipexole inhibits astrocytic NLRP3 inflammasome activation via Drd3-dependent autophagy in a mouse model of Parkinson's disease
    Authors: AQ Dong, YP Yang, SM Jiang, XY Yao, D Qi, CJ Mao, XY Cheng, F Wang, LF Hu, CF Liu
    Acta pharmacologica Sinica, 2022-07-27;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  43. Activation of NLRP3 Is Required for a Functional and Beneficial Microglia Response after Brain Trauma
    Authors: AB Lopez-Rodr, C Decouty-Pe, V Farré-Alin, A Palomino-A, P Narros-Fer, J Egea
    Pharmaceutics, 2022-07-26;14(8):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  44. Therapeutic effect of NLRP3 inhibition on hearing loss induced by systemic inflammation in a CAPS-associated mouse model
    Authors: JH Ma, E Lee, SH Yoon, H Min, JH Oh, I Hwang, Y Sung, JH Ryu, J Bok, JW Yu
    EBioMedicine, 2022-07-20;82(0):104184.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  45. Allergen protease-activated stress granule assembly and gasdermin D fragmentation control interleukin-33 secretion
    Authors: W Chen, S Chen, C Yan, Y Zhang, R Zhang, M Chen, S Zhong, W Fan, S Zhu, D Zhang, X Lu, J Zhang, Y Huang, L Zhu, X Li, D Lv, Y Fu, H Iv, Z Ling, L Ma, H Jiang, G Long, J Zhu, D Wu, B Wu, B Sun
    Nature Immunology, 2022-07-06;23(7):1021-1030.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  46. Kir2.1-mediated membrane potential promotes nutrient acquisition and inflammation through regulation of nutrient transporters
    Authors: W Yu, Z Wang, X Yu, Y Zhao, Z Xie, K Zhang, Z Chi, S Chen, T Xu, D Jiang, X Guo, M Li, J Zhang, H Fang, D Yang, Y Guo, X Yang, X Zhang, Y Wu, W Yang, D Wang
    Nature Communications, 2022-06-21;13(1):3544.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  47. Novel role for caspase 1 inhibitor VX765 in suppressing NLRP3 inflammasome assembly and atherosclerosis via promoting mitophagy and efferocytosis
    Authors: Y Jin, Y Liu, L Xu, J Xu, Y Xiong, Y Peng, K Ding, S Zheng, N Yang, Z Zhang, L Li, L Tan, H Song, J Fu
    Cell Death & Disease, 2022-05-31;13(5):512.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  48. TH17 cells promote CNS inflammation by sensing danger signals via Mincle
    Authors: Q Zhang, W Liu, H Wang, H Zhou, K Bulek, X Chen, CJ Zhang, J Zhao, R Zhang, C Liu, Z Kang, RA Bermel, G Dubyak, DW Abbott, TS Xiao, LE Nagy, X Li
    Nature Communications, 2022-05-03;13(1):2406.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  49. Mitochondrial electron transport chain is necessary for NLRP3 inflammasome activation
    Authors: LK Billingham, JS Stoolman, K Vasan, AE Rodriguez, TA Poor, M Szibor, HT Jacobs, CR Reczek, A Rashidi, P Zhang, J Miska, NS Chandel
    Nature Immunology, 2022-04-28;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  50. Amyloid-beta activates NLRP3 inflammasomes by affecting microglial immunometabolism through the Syk-AMPK pathway
    Authors: ES Jung, K Suh, J Han, H Kim, HS Kang, WS Choi, I Mook-Jung
    Aging Cell, 2022-04-27;0(0):e13623.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  51. Farrerol Alleviates Myocardial Ischemia/Reperfusion Injury by Targeting Macrophages and NLRP3
    Authors: L Zhou, S Yang, X Zou
    Frontiers in Pharmacology, 2022-04-13;13(0):879232.
    Species: Mouse
    Sample Types: Tissue Lysates
    Applications: Western Blot
  52. BMAL1 regulates Propionibacterium acnes-induced skin inflammation via REV-ERBalpha in mice
    Authors: F Li, L Lin, Y He, G Sun, D Dong, B Wu
    International journal of biological sciences, 2022-03-21;18(6):2597-2608.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  53. Motoneuronal inflammasome activation triggers excessive neuroinflammation and impedes regeneration after sciatic nerve injury
    Authors: K Molnár, B Nógrádi, R Kristóf, Á Mészáros, K Pajer, L Siklós, A Nógrádi, I Wilhelm, IA Krizbai
    Journal of Neuroinflammation, 2022-03-19;19(1):68.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  54. Inflammasome NLRP3 activation induced by Convulxin, a C-type lectin-like isolated from Crotalus durissus terrificus snake venom
    Authors: CMA Rego, AF Francisco, CN Boeno, MV Paloschi, JA Lopes, MDS Silva, HM Santana, SN Serrath, JE Rodrigues, CTL Lemos, RSS Dutra, JN da Cruz, CBR Dos Santos, S da S Setúb, MRM Fontes, AM Soares, WL Pires, JP Zuliani
    Scientific Reports, 2022-03-18;12(1):4706.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA
  55. Hyperphosphatemia increases inflammation to exacerbate anemia and skeletal muscle wasting independently of FGF23-FGFR4 signaling
    Authors: B Czaya, K Heitman, I Campos, C Yanucil, D Kentrup, D Westbrook, O Gutierrez, JL Babitt, G Jung, IB Salusky, M Hanudel, C Faul
    Elife, 2022-03-18;11(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  56. CrkII/Abl phosphorylation cascade is critical for NLRC4 inflammasome activity and is blocked by Pseudomonas aeruginosa ExoT
    Authors: MF Mohamed, K Gupta, JW Goldufsky, R Roy, LT Callaghan, DM Wetzel, TM Kuzel, J Reiser, SH Shafikhani
    Nature Communications, 2022-03-11;13(1):1295.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  57. Nonredundancy of IL-1alpha and IL-1beta is defined by distinct regulation of tissues orchestrating resistance versus tolerance to infection
    Authors: K Eislmayr, A Bestehorn, L Morelli, M Borroni, LV Walle, M Lamkanfi, P Kovarik
    Science Advances, 2022-03-02;8(9):eabj7293.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  58. K48/K63-linked polyubiquitination of ATG9A by TRAF6 E3 ligase regulates oxidative stress-induced autophagy
    Authors: YT Wang, TY Liu, CH Shen, SY Lin, CC Hung, LC Hsu, GC Chen
    Cell Reports, 2022-02-22;38(8):110354.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  59. Discovery of a novel and potent inhibitor with differential species-specific effects against NLRP3 and AIM2 inflammasome-dependent pyroptosis
    Authors: Y Jiao, J Nan, B Mu, Y Zhang, N Zhou, S Yang, S Zhang, W Lin, F Wang, A Xia, Z Cao, P Chen, Z Pan, G Lin, S Pan, H Bin, L Li, S Yang
    European Journal of Medicinal Chemistry, 2022-02-11;232(0):114194.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  60. Ubiquitination of NLRP3 by gp78/Insig-1 restrains NLRP3 inflammasome activation
    Authors: T Xu, W Yu, H Fang, Z Wang, Z Chi, X Guo, D Jiang, K Zhang, S Chen, M Li, Y Guo, J Zhang, D Yang, Q Yu, D Wang, X Zhang
    Cell Death and Differentiation, 2022-02-02;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  61. The NKCC1 ion transporter modulates microglial phenotype and inflammatory response to brain injury in a cell-autonomous manner
    Authors: K Tóth, N Lénárt, P Berki, R Fekete, E Szabadits, B Pósfai, C Cserép, A Alatshan, S Benk?, D Kiss, CA Hübner, A Gulyás, K Kaila, Z Környei, Á Dénes
    PloS Biology, 2022-01-27;20(1):e3001526.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  62. TREM-1 is required for enhanced OpZ-induced superoxide generation following priming
    Authors: S Murthy, S Baruah, JL Bowen, K Keck, BA Wagner, GR Buettner, DB Sykes, J Klesney-Ta
    Journal of leukocyte biology, 2022-01-24;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  63. Maternal P2X7 receptor inhibition prevents autism-like phenotype in male mouse offspring through the NLRP3-IL-1beta pathway
    Authors: D Szabó, P Tod, F Gölöncsér, V Román, B Lendvai, L Otrokocsi, B Sperlágh
    Brain, Behavior, and Immunity, 2022-01-20;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  64. Activation of Transcription Factor EB Alleviates Tubular Epithelial Cell Injury via Restoring Lysosomal Homeostasis in Diabetic Nephropathy
    Authors: S Wang, K Jing, H Wu, X Li, C Yang, T Li, H Tang, T Zou, Y She, HF Liu
    Oxidative Medicine and Cellular Longevity, 2022-01-12;2022(0):2812493.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  65. Bisphosphonate drugs have actions in the lung and inhibit the mevalonate pathway in alveolar macrophages
    Authors: MA Munoz, EK Fletcher, OP Skinner, J Jurczyluk, E Kristianto, MP Hodson, S Sun, FH Ebetino, DR Croucher, PM Hansbro, JR Center, MJ Rogers
    Elife, 2021-12-30;10(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  66. Signalling metabolite L-2-hydroxyglutarate activates the transcription factor HIF-1alpha in lipopolysaccharide-activated macrophages
    Authors: NC Williams, DG Ryan, ASH Costa, EL Mills, MP Jedrychows, SM Cloonan, C Frezza, LA O'Neill
    The Journal of Biological Chemistry, 2021-12-17;0(0):101501.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  67. Sensory nerves promote corneal inflammation resolution via CGRP mediated transformation of macrophages to the M2 phenotype through the PI3K/AKT signaling pathway
    Authors: K Yuan, J Zheng, X Shen, Y Wu, Y Han, X Jin, X Huang
    International immunopharmacology, 2021-12-11;102(0):108426.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  68. Dihydrotanshinone I Specifically Inhibits NLRP3 Inflammasome Activation and Protects Against Septic Shock In Vivo
    Authors: Z Wei, X Zhan, K Ding, G Xu, W Shi, L Ren, Z Fang, T Liu, X Hou, J Zhao, H Li, J Li, Z Li, Q Li, L Lin, Y Yang, X Xiao, Z Bai, J Cao
    Frontiers in Pharmacology, 2021-10-14;12(0):750815.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  69. Parabens disrupt non-canonical inflammasome activation
    Authors: H Ahn, H Lee, G Lee, J Park, HW Sung, E Lee, GS Lee
    International immunopharmacology, 2021-09-30;101(0):108196.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  70. Antiatherosclerotic effect of dehydrocorydaline on ApoE-/- mice: inhibition of macrophage inflammation
    Authors: B Wen, YY Dang, SH Wu, YM Huang, KY Ma, YM Xu, XL Zheng, XY Dai
    Acta pharmacologica Sinica, 2021-09-22;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  71. Proteopathic tau primes and activates interleukin-1beta via myeloid-cell-specific MyD88- and NLRP3-ASC-inflammasome pathway
    Authors: S Jiang, NM Maphis, J Binder, D Chisholm, L Weston, W Duran, C Peterson, A Zimmerman, MA Mandell, SD Jett, E Bigio, C Geula, N Mellios, JP Weick, GA Rosenberg, E Latz, MT Heneka, K Bhaskar
    Cell Reports, 2021-09-21;36(12):109720.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  72. Chronic colitis upregulates microRNAs suppressing brain-derived neurotrophic factor in the adult heart
    Authors: Y Tang, KT Kline, XS Zhong, Y Xiao, H Lian, J Peng, X Liu, DW Powell, G Tang, Q Li
    PLoS ONE, 2021-09-20;16(9):e0257280.
    Species: Rat
    Sample Types: In Vivo
    Applications: Neutralization
  73. The Essential Oil of Artemisia argyi H.L�v. and Vaniot Attenuates NLRP3 Inflammasome Activation in THP-1 Cells
    Authors: P Chen, Q Bai, Y Wu, Q Zeng, X Song, Y Guo, P Zhou, Y Wang, X Liao, Q Wang, Z Ren, Y Wang
    Frontiers in Pharmacology, 2021-09-16;12(0):712907.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  74. Macrophage HIF-2alpha suppresses NLRP3 inflammasome activation and alleviates insulin resistance
    Authors: X Li, X Zhang, J Xia, L Zhang, B Chen, G Lian, C Yun, J Yang, Y Yan, P Wang, X Wang, B Liu, H Liu, H Liang, Y Pang, X Wang, C Jiang
    Cell Reports, 2021-08-24;36(8):109607.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: ChIP, ICC, Western Blot
  75. Immunohistochemical Study of ASC Expression and Distribution in the Hippocampus of an Aged Murine Model of Alzheimer's Disease
    Authors: D Reimers, M Vallejo-Mu, MJ Casarejos, A Jimenez-Es, R Gonzalo-Go, E Bazan
    International Journal of Molecular Sciences, 2021-08-13;22(16):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  76. Co-Treatment With Verapamil and Curcumin Attenuates the Behavioral Alterations Observed in Williams-Beuren Syndrome Mice by Regulation of MAPK Pathway and Microglia Overexpression
    Authors: P Ortiz-Rome, A González-S, G Egea, LA Pérez-Jura, V Campuzano
    Frontiers in Pharmacology, 2021-08-03;12(0):670785.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  77. ML365 inhibits TWIK2 channel to block ATP-induced NLRP3 inflammasome
    Authors: XY Wu, JY Lv, SQ Zhang, X Yi, ZW Xu, YX Zhi, BX Zhao, JX Pang, KKL Yung, SW Liu, PZ Zhou
    Acta pharmacologica Sinica, 2021-08-02;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  78. Mycobacterium tuberculosis inhibits the NLRP3 inflammasome activation via its phosphokinase PknF
    Authors: S Rastogi, S Ellinwood, J Augenstrei, KD Mayer-Barb, V Briken
    PloS Pathogens, 2021-07-29;17(7):e1009712.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  79. A phenotypic high-content, high-throughput screen identifies inhibitors of NLRP3 inflammasome activation
    Authors: S Nizami, V Millar, K Arunasalam, T Zarganes-T, D Brough, G Tresadern, PE Brennan, JB Davis, D Ebner, E Di Daniel
    Scientific Reports, 2021-07-28;11(1):15319.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  80. NLRP3 Triggers Attenuate Lipocalin-2 Expression Independent with Inflammasome Activation
    Authors: H Ahn, G Lee, J Kim, J Park, SG Kang, SI Yoon, E Lee, GS Lee
    Cells, 2021-07-02;10(7):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  81. Terminal uridyltransferase 7 regulates TLR4-triggered inflammation by controlling Regnase-1 mRNA uridylation and degradation
    Authors: CC Lin, YR Shen, CC Chang, XY Guo, YY Young, TY Lai, IS Yu, CY Lee, TH Chuang, HY Tsai, LC Hsu
    Nature Communications, 2021-06-29;12(1):3878.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  82. &beta-Glucan-stimulated neutrophil secretion of IL-1&alpha is independent of GSDMD and mediated through extracellular vesicles
    Authors: B Ratitong, M Marshall, E Pearlman
    Cell Reports, 2021-05-18;35(7):109139.
    Species: Human
    Sample Types: Transfected Whole Cells
    Applications: Neutralization
  83. The ubiquitylation of IL-1&beta limits its cleavage by caspase-1 and targets it for proteasomal degradation
    Authors: SL Vijayaraj, R Feltham, M Rashidi, D Frank, Z Liu, DS Simpson, G Ebert, A Vince, MJ Herold, A Kueh, JS Pearson, LF Dagley, JM Murphy, AI Webb, KE Lawlor, JE Vince
    Nature Communications, 2021-05-11;12(1):2713.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  84. Gasdermin D pore structure reveals preferential release of mature interleukin-1
    Authors: S Xia, Z Zhang, VG Magupalli, JL Pablo, Y Dong, SM Vora, L Wang, TM Fu, MP Jacobson, A Greka, J Lieberman, J Ruan, H Wu
    Nature, 2021-04-21;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  85. A small molecule binding HMGB1 inhibits caspase-11-mediated lethality in sepsis
    Authors: X Wang, Z Li, Y Bai, R Zhang, R Meng, F Chen, H Wang, TR Billiar, X Xiao, B Lu, Y Tang
    Cell Death & Disease, 2021-04-14;12(4):402.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: Flow Cytometry, Proximity Ligation Assay, Western Blot
  86. Gasdermin E permits interleukin-1 beta release in distinct sublytic and pyroptotic phases
    Authors: B Zhou, DW Abbott
    Cell Reports, 2021-04-13;35(2):108998.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  87. Vaccine delivery alerts innate immune systems for more immunogenic vaccination
    Authors: Z Li, Y Cao, Y Li, Y Zhao, X Chen
    JCI Insight, 2021-04-08;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  88. Discovery of chalcone analogues as novel NLRP3 inflammasome inhibitors with potent anti-inflammation activities
    Authors: C Zhang, H Yue, P Sun, L Hua, S Liang, Y Ou, D Wu, X Wu, H Chen, Y Hao, W Hu, Z Yang
    European Journal of Medicinal Chemistry, 2021-04-01;219(0):113417.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  89. Increased cytokine expression in the choroid plexus stroma and epithelium in response to endotoxin-induced systemic inflammation in mice
    Authors: A Shimada, S Hasegawa-I
    Toxicology reports, 2021-03-10;8(0):520-528.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  90. Luteolin inhibits NLRP3 inflammasome activation via blocking ASC oligomerization
    Authors: MN Lee, Y Lee, D Wu, M Pae
    The Journal of nutritional biochemistry, 2021-03-08;0(0):108614.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  91. Sex-dependent differences in the gut microbiota following chronic nasal inflammation in adult mice
    Authors: Y Mishima, T Osaki, A Shimada, S Kamiya, S Hasegawa-I
    Scientific Reports, 2021-02-25;11(1):4640.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  92. Omega-3FAs Can Inhibit the Inflammation and Insulin Resistance of Adipose Tissue Caused by HHcy Induced Lipids Profile Changing in Mice
    Authors: J Li, H Zhang, Y Dong, X Wang, G Wang
    Frontiers in Physiology, 2021-02-12;12(0):628122.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  93. NLRP3 inflammasome inhibitor INF39 attenuated NLRP3 assembly in macrophages
    Authors: Y Shi, Q Lv, M Zheng, H Sun, F Shi
    International immunopharmacology, 2021-01-25;92(0):107358.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  94. Gasdermin D restricts Burkholderia cenocepacia infection in vitro and in vivo
    Authors: S Estfanous, K Krause, MNK Anne, M Eltobgy, K Caution, A Abu Khweek, K Hamilton, A Badr, K Daily, C Carafice, D Baetzhold, X Zhang, T Li, H Wen, MA Gavrilin, H Haffez, S Soror, AO Amer
    Scientific Reports, 2021-01-13;11(1):855.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  95. Inhibiting NLRP3 inflammasome attenuates apoptosis in contrast-induced acute kidney injury through the upregulation of HIF1A and BNIP3-mediated mitophagy
    Authors: Q Lin, S Li, N Jiang, H Jin, X Shao, X Zhu, J Wu, M Zhang, Z Zhang, J Shen, W Zhou, L Gu, R Lu, Z Ni
    Autophagy, 2020-12-19;0(0):1-16.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  96. NKT cells promote both type 1 and type 2 inflammatory responses in a mouse model of liver fibrosis
    Authors: J Nilsson, M Hörnberg, A Schmidt-Ch, K Linde, M Nilsson, M Carlus, SF Erttmann, S Mayans, D Holmberg
    Scientific Reports, 2020-12-11;10(1):21778.
    Species: Mouse
    Sample Types: Tissue Lysates
    Applications: Western Blot
  97. Paxillin mediates ATP-induced activation of P2X7 receptor and NLRP3 inflammasome
    Authors: W Wang, D Hu, Y Feng, C Wu, Y Song, W Liu, A Li, Y Wang, K Chen, M Tian, F Xiao, Q Zhang, W Chen, P Pan, P Wan, Y Liu, H Lan, K Wu, J Wu
    BMC Biol, 2020-11-26;18(1):182.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  98. Upregulation of Nucleotide-Binding Oligomerization Domain-, LRR- and Pyrin Domain-Containing Protein 3 in Motoneurons Following Peripheral Nerve Injury in Mice
    Authors: B Nógrádi, Á Nyúl-Tóth, M Kozma, K Molnár, R Patai, L Siklós, I Wilhelm, IA Krizbai
    Frontiers in Pharmacology, 2020-11-26;11(0):584184.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  99. Myeloid Kr�ppel-like factor 2 is a critical regulator of metabolic inflammation
    Authors: DR Sweet, NT Vasudevan, L Fan, CE Booth, KS Keerthy, X Liao, V Vinayachan, Y Takami, D Tugal, N Sharma, ER Chan, L Zhang, Y Qing, SL Gerson, C Fu, A Wynshaw-Bo, P Sangwung, L Nayak, P Holvoet, K Matoba, Y Lu, G Zhou, MK Jain
    Nat Commun, 2020-11-18;11(1):5872.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  100. Synthetic lethality between MyD88 loss and mutations in Wnt/&beta-catenin pathway in intestinal tumor epithelial cells
    Authors: R Kajino-Sak, T Fujishita, MM Taketo, M Aoki
    Oncogene, 2020-11-12;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  101. Riboflavin, vitamin B2, attenuates NLRP3, NLRC4, AIM2, and non-canonical inflammasomes by the inhibition of caspase-1 activity
    Authors: H Ahn, GS Lee
    Sci Rep, 2020-11-05;10(1):19091.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  102. Inhibition of NLRP3 inflammasome by MCC950 improves the metabolic outcome of islet transplantation by suppressing IL-1&beta and islet cellular death
    Authors: T Matsuoka, G Yoshimatsu, N Sakata, R Kawakami, T Tanaka, T Yamada, Y Yoshida, S Hasegawa, S Kodama
    Sci Rep, 2020-10-21;10(1):17920.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  103. Secretory autophagy machinery and vesicular trafficking are involved in HMGB1 secretion
    Authors: YH Kim, MS Kwak, B Lee, JM Shin, S Aum, IH Park, MG Lee, JS Shin
    Autophagy, 2020-10-05;0(0):1-18.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  104. Pristimerin protects against inflammation and metabolic disorder in mice through inhibition of NLRP3 inflammasome activation
    Authors: Q Zhao, Y Bi, J Guo, YX Liu, J Zhong, LR Pan, Y Tan, XJ Yu
    Acta Pharmacol Sin, 2020-09-28;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation, Western Blot
  105. Indirect regulation of HMGB1 release by gasdermin D
    Authors: A Volchuk, A Ye, L Chi, BE Steinberg, NM Goldenberg
    Nat Commun, 2020-09-11;11(1):4561.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  106. Metabolic competition between host and pathogen dictates inflammasome responses to fungal infection
    Authors: TM Tucey, J Verma, FAB Olivier, TL Lo, AAB Robertson, T Naderer, A Traven
    PLoS Pathog., 2020-08-04;16(8):e1008695.
    Species: Human, Mouse
    Sample Types: Cell Culture Supernates, Cell Lysates
    Applications: Western Blot
  107. Epithelial-derived gasdermin D mediates nonlytic IL-1&beta release during experimental colitis
    Authors: K Bulek, J Zhao, Y Liao, N Rana, D Corridoni, A Antanavici, X Chen, H Wang, W Qian, WA Miller-Lit, S Swaidani, F Tang, BB Willard, K McCrae, Z Kang, GR Dubyak, F Cominelli, A Simmons, TT Pizarro, X Li
    J. Clin. Invest., 2020-08-03;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: IP
  108. Slc6a3-dependent expression of a CAPS-associated Nlrp3 allele results in progressive behavioral abnormalities and neuroinflammation in aging mice
    Authors: KM von Herrma, FL Anderson, EM Martinez, AL Young, MC Havrda
    J Neuroinflammation, 2020-07-17;17(1):213.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  109. IL-1beta suppression of VE-cadherin transcription underlies sepsis-induced inflammatory lung injury
    Authors: S Xiong, Z Hong, LS Huang, Y Tsukasaki, S Nepal, A Di, M Zhong, W Wu, Z Ye, X Gao, GN Rao, D Mehta, J Rehman, AB Malik
    J. Clin. Invest., 2020-07-01;130(7):3684-3698.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation, Western Blot
  110. Ancient familial Mediterranean fever mutations in human pyrin and resistance to Yersinia pestis
    Authors: YH Park, EF Remmers, W Lee, AK Ombrello, LK Chung, Z Shilei, DL Stone, MI Ivanov, NA Loeven, KS Barron, P Hoffmann, M Nehrebecky, YZ Akkaya-Ulu, E Sag, B Balci-Peyn, I Aksentijev, A Gül, CN Rotimi, H Chen, JB Bliska, S Ozen, DL Kastner, D Shriner, JJ Chae
    Nat. Immunol., 2020-06-29;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  111. Myeloid PTEN promotes chemotherapy-induced NLRP3-inflammasome activation and antitumour immunity
    Authors: Y Huang, H Wang, Y Hao, H Lin, M Dong, J Ye, L Song, Y Wang, Q Li, B Shan, Y Jiang, H Li, Z Shao, G Kroemer, H Zhang, L Bai, T Jin, C Wang, Y Ma, Y Cai, C Ding, S Liu, Y Pan, W Jiang, R Zhou
    Nat. Cell Biol., 2020-05-04;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  112. A Two-Cell Model for IL-1&beta Release Mediated by Death-Receptor Signaling
    Authors: CA Donado, AB Cao, DP Simmons, BA Croker, PJ Brennan, MB Brenner
    Cell Rep, 2020-04-07;31(1):107466.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Western Blot
  113. ELMO2 association with G&alphai2 regulates pancreatic cancer cell chemotaxis and metastasis
    Authors: Y Wang, H Li, F Li
    PeerJ, 2020-04-06;8(0):e8910.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  114. Oncogenic KrasG12D causes myeloproliferation via NLRP3 inflammasome activation
    Authors: S Hamarsheh, L Osswald, BS Saller, S Unger, D De Feo, JM Vinnakota, M Konantz, FM Uhl, H Becker, M Lübbert, K Shoumariye, C Schürch, G Andrieux, N Venhoff, A Schmitt-Gr, S Duquesne, D Pfeifer, MA Cooper, C Lengerke, M Boerries, J Duyster, CM Niemeyer, M Erlacher, BR Blazar, B Becher, O Gro beta, T Brummer, R Zeiser
    Nat Commun, 2020-04-03;11(1):1659.
    Species: Mouse
    Sample Types: Cell Culture Lysates
    Applications: Western Blot
  115. Inhibition of double-strand DNA-sensing cGAS ameliorates brain injury after ischemic stroke
    Authors: Q Li, Y Cao, C Dang, B Han, R Han, H Ma, J Hao, L Wang
    EMBO Mol Med, 2020-04-01;12(4):e11002.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  116. Mycobacterium abscessus infection leads to enhanced production of type 1 interferon and NLRP3 inflammasome activation in murine macrophages via mitochondrial oxidative stress
    Authors: BR Kim, BJ Kim, YH Kook, BJ Kim
    PLoS Pathog., 2020-03-25;16(3):e1008294.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  117. Involvement of exchange protein directly activated by cAMP and tumor progression locus 2 in IL-1&beta production in microglial cells following activation of &beta-adrenergic receptors
    Authors: H Tozaki-Sai, I Sasaki, T Yamashita, M Hosoi, TA Kato, M Tsuda
    J. Pharmacol. Sci., 2020-03-21;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  118. Suppression of NLRP3 Inflammasome by Erythropoietin via the EPOR/JAK2/STAT3 Pathway Contributes to Attenuation of Acute Lung Injury in Mice
    Authors: F Cao, X Tian, Z Li, Y Lv, J Han, R Zhuang, B Cheng, Y Gong, B Ying, S Jin, Y Gao
    Front Pharmacol, 2020-03-19;11(0):306.
    Species: Mouse
    Sample Types: Tissue Lysates
    Applications: Western Blot
  119. Slco2a1 deficiency exacerbates experimental colitis via inflammasome activation in macrophages: a possible mechanism of chronic enteropathy associated with SLCO2A1 gene
    Authors: R Nakata, Y Nakamura, S Hosomi, H Okuda, Y Nishida, N Sugita, S Itani, Y Nadatani, K Otani, F Tanaka, N Kamata, K Taira, Y Nagami, T Tanigawa, T Watanabe, H Yamagami, T Nakanishi, Y Fujiwara
    Sci Rep, 2020-03-17;10(1):4883.
    Species: Mouse
    Sample Types: Cell Culture Lysates
    Applications: Western Blot
  120. Genetic and pharmacological inhibition of inflammasomes reduces the survival of Mycobacterium tuberculosis strains in macrophages
    Authors: S Subbarao, J Sanchez-Ga, N Krishnan, AR Shenoy, BD Robertson
    Sci Rep, 2020-02-28;10(1):3709.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  121. Caspase-11 promotes allergic airway inflammation
    Authors: Z Zas?ona, E Flis, MM Wilk, RG Carroll, EM Palsson-Mc, MM Hughes, C Diskin, K Banahan, DG Ryan, A Hooftman, A Misiak, J Kearney, G Lochnit, W Bertrams, T Greulich, B Schmeck, OJ McElvaney, KHG Mills, EC Lavelle, M Wygrecka, EM Creagh, LAJ O'Neill
    Nat Commun, 2020-02-26;11(1):1055.
    Species: Human
    Sample Types: Cell Culture Lysates
    Applications: Western Blot
  122. Class IIa Histone Deacetylases Drive Toll-like Receptor-Inducible Glycolysis and Macrophage Inflammatory Responses via Pyruvate Kinase M2
    Authors: K Das Gupta, MR Shakespear, JEB Curson, AMV Murthy, A Iyer, MP Hodson, D Ramnath, VA Tillu, JB von Pein, RC Reid, K Tunny, DM Hohenhaus, SV Moradi, GM Kelly, T Kobayashi, JH Gunter, AJ Stevenson, W Xu, L Luo, A Jones, WA Johnston, A Blumenthal, K Alexandrov, BM Collins, JL Stow, DP Fairlie, MJ Sweet
    Cell Rep, 2020-02-25;30(8):2712-2728.e8.
    Species: Mouse
    Sample Types: Cell Culture Lysates
    Applications: Western Blot
  123. Protective Role of Shiitake Mushroom-Derived Exosome-Like Nanoparticles in D-Galactosamine and Lipopolysaccharide-Induced Acute Liver Injury in Mice
    Authors: B Liu, Y Lu, X Chen, PG Muthuraj, X Li, M Pattabiram, J Zempleni, SD Kachman, SK Natarajan, J Yu
    Nutrients, 2020-02-13;12(2):.
    Species: Mouse
    Sample Types: Tissue Lysates
    Applications: Western Blot
  124. Caspase-1-dependent inflammasomes mediate photoreceptor cell death in photo-oxidative damage-induced retinal degeneration
    Authors: Y Wooff, N Fernando, JHC Wong, C Dietrich, R Aggio-Bruc, JA Chu-Tan, AAB Robertson, SL Doyle, SM Man, R Natoli
    Sci Rep, 2020-02-10;10(1):2263.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  125. Bacillus cereus non-haemolytic enterotoxin activates the NLRP3 inflammasome
    Authors: D Fox, A Mathur, Y Xue, Y Liu, WH Tan, S Feng, A Pandey, C Ngo, JA Hayward, II Atmosukart, JD Price, MD Johnson, N Jessberger, AAB Robertson, G Burgio, DC Tscharke, EM Fox, DL Leyton, NO Kaakoush, E Märtlbauer, SH Leppla, SM Man
    Nat Commun, 2020-02-06;11(1):760.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  126. I&kappaB? controls NLRP3 inflammasome activation via upregulation of the Nlrp3 gene
    Authors: J Kim, H Ahn, S Yu, JH Ahn, HJ Ko, MN Kweon, EJ Hong, BS An, E Lee, GS Lee
    Cytokine, 2020-01-07;127(0):154983.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  127. A Mitochondrial Micropeptide Is Required for Activation of the Nlrp3 Inflammasome
    Authors: A Bhatta, M Atianand, Z Jiang, J Crabtree, J Blin, KA Fitzgerald
    J. Immunol., 2019-12-13;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  128. Identification of hub genes and key pathways of dietary advanced glycation end products?induced non?alcoholic fatty liver disease by bioinformatics analysis and animal experiments
    Authors: J Wang, H Liu, G Xie, W Cai, J Xu
    Mol Med Rep, 2019-12-09;21(2):685-694.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  129. Ptpn6 inhibits caspase-8- and Ripk3/Mlkl-dependent inflammation
    Authors: M Speir, CJ Nowell, AA Chen, JA O'Donnell, IS Shamie, PR Lakin, AA D'Cruz, RO Braun, JJ Babon, RS Lewis, M Bliss-More, I Shlomovitz, S Wang, LH Cengia, AI Stoica, R Hakem, MA Kelliher, LA O'Reilly, H Patsiouras, KE Lawlor, E Weller, NE Lewis, AW Roberts, M Gerlic, BA Croker
    Nat. Immunol., 2019-12-09;21(1):54-64.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  130. Endogenous oxidized phospholipids reprogram cellular metabolism and boost hyperinflammation
    Authors: M Di Gioia, R Spreafico, JR Springstea, MM Mendelson, R Joehanes, D Levy, I Zanoni
    Nat. Immunol., 2019-11-25;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  131. IL-25 exacerbates autoimmune aortitis in IL-1 receptor antagonist-deficient mice
    Authors: T Yoshizaki, S Itoh, S Yamaguchi, T Numata, A Nambu, N Kimura, H Suto, K Okumura, K Sudo, A Yamaguchi, S Nakae
    Sci Rep, 2019-11-19;9(1):17067.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  132. Gasdermin D Drives the Nonexosomal Secretion of Galectin-3, an Insulin Signal Antagonist
    Authors: Y Chen, H Wang, J Shen, R Deng, X Yao, Q Guo, A Lu, B Sun, Y Zhang, G Meng
    J. Immunol., 2019-10-09;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  133. Glutathione Transferase Omega-1 Regulates NLRP3 Inflammasome Activation through NEK7 Deglutathionylation
    Authors: MM Hughes, A Hooftman, S Angiari, P Tummala, Z Zaslona, MC Runtsch, AF McGettrick, CE Sutton, C Diskin, M Rooke, S Takahashi, S Sundararaj, MG Casarotto, JE Dahlstrom, EM Palsson-Mc, SC Corr, KHG Mills, RJS Preston, N Neamati, Y Xie, JB Baell, PG Board, LAJ O'Neill
    Cell Rep, 2019-10-01;29(1):151-161.e5.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  134. CCL28 promotes locomotor recovery after spinal cord injury via recruiting regulatory T cells
    Authors: P Wang, X Qi, G Xu, J Liu, J Guo, X Li, X Ma, H Sun
    Aging (Albany NY), 2019-09-26;11(18):7402-7415.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  135. DDX3X acts as a live-or-die checkpoint in stressed cells by regulating NLRP3 inflammasome
    Authors: P Samir, S Kesavardha, DM Patmore, S Gingras, RKS Malireddi, R Karki, CS Guy, B Briard, DE Place, A Bhattachar, BR Sharma, A Nourse, SV King, A Pitre, AR Burton, S Pelletier, RJ Gilbertson, TD Kanneganti
    Nature, 2019-09-11;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  136. Protein prenylation restrains innate immunity by inhibiting Rac1 effector interactions
    Authors: MK Akula, MX Ibrahim, EG Ivarsson, OM Khan, IT Kumar, M Erlandsson, C Karlsson, X Xu, M Brisslert, C Brakebusch, D Wang, M Bokarewa, VI Sayin, MO Bergo
    Nat Commun, 2019-09-04;10(1):3975.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  137. Chaetocin attenuates gout in mice through inhibiting HIF-1? and NLRP3 inflammasome-dependent IL-1? secretion in macrophages
    Authors: Mian Wu, M Zhang, Y Ma, F Liu, S Chen, J Lu, H Chen
    Arch. Biochem. Biophys., 2019-06-28;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  138. PINK1-parkin pathway of mitophagy protects against contrast-induced acute kidney injury via decreasing mitochondrial ROS and NLRP3 inflammasome activation
    Authors: Q Lin, S Li, N Jiang, X Shao, M Zhang, H Jin, Z Zhang, J Shen, Y Zhou, W Zhou, L Gu, R Lu, Z Ni
    Redox Biol, 2019-06-11;26(0):101254.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  139. Protein tyrosine phosphatase non-receptor type 22 modulates colitis in a microbiota-dependent manner
    Authors: MR Spalinger, TS Schmidt, M Schwarzfis, L Hering, K Atrott, S Lang, C Gottier, A Geirnaert, C Lacroix, X Dai, DJ Rawlings, AC Chan, C von Mering, G Rogler, M Scharl
    J. Clin. Invest., 2019-05-20;130(0):2527-2541.
    Species: Mouse
    Sample Types: Tissue Lysates
    Applications: Western Blot
  140. RNA viruses promote activation of the NLRP3 inflammasome through cytopathogenic effect-induced potassium efflux
    Authors: LS da Costa, A Outlioua, A Anginot, K Akarid, D Arnoult
    Cell Death Dis, 2019-04-25;10(5):346.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  141. Dicer regulates activation of the NLRP3 inflammasome
    Authors: DM Ojcius, A Jafari, L Yeruva, CW Schindler, AA Abdul-Sate
    PLoS ONE, 2019-04-23;14(4):e0215689.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  142. High mobility group box 1 enables bacterial lipids to trigger receptor-interacting protein kinase 3 (RIPK3)-mediated necroptosis and apoptosis in mice
    Authors: R Meng, L Gu, Y Lu, K Zhao, J Wu, H Wang, J Han, Y Tang, B Lu
    J. Biol. Chem., 2019-04-18;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  143. The lncRNA Neat1 promotes activation of inflammasomes in macrophages
    Authors: P Zhang, L Cao, R Zhou, X Yang, M Wu
    Nat Commun, 2019-04-02;10(1):1495.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  144. The three cytokines IL-1?, IL-18, and IL-1? share related but distinct secretory routes
    Authors: VS Tapia, MJD Daniels, P Palazón-Ri, M Dewhurst, NM Luheshi, J Rivers-Aut, J Green, E Redondo-Ca, P Kaldis, G Lopez-Cast, D Brough
    J. Biol. Chem., 2019-04-02;294(21):8325-8335.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  145. Long noncoding RNA Malat1 regulates differential activation of macrophage and response to lung injury
    Authors: H Cui, S Banerjee, S Guo, N Xie, J Ge, D Jiang, M Zörnig, VJ Thannickal, G Liu
    JCI Insight, 2019-02-21;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  146. Responses of perivascular macrophages to circulating lipopolysaccharides in the subfornical organ with special reference to endotoxin tolerance
    Authors: S Morita-Tak, K Nakahara, S Hasegawa-I, A Isonishi, K Tatsumi, H Okuda, T Tanaka, M Kitabatake, T Ito, A Wanaka
    J Neuroinflammation, 2019-02-14;16(1):39.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  147. Macrophages, rather than DCs, are responsible for inflammasome activity in the GM-CSF BMDC model
    Authors: Z Erlich, I Shlomovitz, L Edry-Botze, H Cohen, D Frank, H Wang, AM Lew, KE Lawlor, Y Zhan, JE Vince, M Gerlic
    Nat. Immunol., 2019-02-11;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  148. Tumor stem-like cell-derived exosomal RNAs prime neutrophils for facilitating tumorigenesis of colon cancer
    Authors: WL Hwang, HY Lan, WC Cheng, SC Huang, MH Yang
    J Hematol Oncol, 2019-01-25;12(1):10.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  149. Injury induces endothelial to mesenchymal transition in the mouse corneal endothelium in vivo via FGF2
    Authors: J Lee, E Jung, M Heur
    Mol. Vis., 2019-01-20;25(0):22-34.
    Species: Mouse
    Sample Types: Aqueous Humor
    Applications: Western Blot
  150. Dynamic role of the codon 72 p53 single-nucleotide polymorphism in mammary tumorigenesis in a humanized mouse model
    Authors: RT Gunaratna, A Santos, L Luo, C Nagi, I Lambertz, M Spier, CJ Conti, RS Fuchs-Youn
    Oncogene, 2019-01-16;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  151. SIRT3 diminishes inflammation and mitigates endotoxin-induced acute lung injury
    Authors: D Kurundkar, AR Kurundkar, NB Bone, EJ Becker, W Liu, B Chacko, V Darley-Usm, JW Zmijewski, VJ Thannickal
    JCI Insight, 2019-01-10;4(1):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  152. The Traditional Chinese Medicine MLC901 inhibits inflammation processes after focal cerebral ischemia
    Authors: C Widmann, C Gandin, A Petit-Pait, M Lazdunski, C Heurteaux
    Sci Rep, 2018-12-24;8(1):18062.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  153. A multicomponent toxin from Bacillus cereus incites inflammation and shapes host outcome via the NLRP3 inflammasome
    Authors: A Mathur, S Feng, JA Hayward, C Ngo, D Fox, II Atmosukart, JD Price, K Schauer, E Märtlbauer, AAB Robertson, G Burgio, EM Fox, SH Leppla, NO Kaakoush, SM Man
    Nat Microbiol, 2018-12-10;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  154. The Mitochondrial Apoptotic Effectors BAX/BAK Activate Caspase-3 and -7 to Trigger NLRP3 Inflammasome and Caspase-8 Driven IL-1? Activation
    Authors: JE Vince, D De Nardo, W Gao, AJ Vince, C Hall, K McArthur, D Simpson, S Vijayaraj, LM Lindqvist, P Bouillet, MA Rizzacasa, SM Man, J Silke, SL Masters, G Lessene, DCS Huang, DHD Gray, BT Kile, F Shao, KE Lawlor
    Cell Rep, 2018-11-27;25(9):2339-2353.e4.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  155. A Metabolic Checkpoint for the Yeast-to-Hyphae Developmental Switch Regulated by Endogenous Nitric Oxide Signaling
    Authors: B Koch, AA Barugahare, TL Lo, C Huang, RB Schittenhe, DR Powell, TH Beilharz, A Traven
    Cell Rep, 2018-11-20;25(8):2244-2258.e7.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  156. TNF/TNFR axis promotes pyrin inflammasome activation and distinctly modulates pyrin inflammasomopathy
    Authors: D Sharma, A Malik, C Guy, P Vogel, TD Kanneganti
    J. Clin. Invest., 2018-11-19;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  157. The fungal peptide toxin Candidalysin activates the NLRP3 inflammasome and causes cytolysis in mononuclear phagocytes
    Authors: L Kasper, A König, PA Koenig, MS Gresnigt, J Westman, RA Drummond, MS Lionakis, O Gro beta, J Ruland, JR Naglik, B Hube
    Nat Commun, 2018-10-15;9(1):4260.
  158. REV-ERB? integrates colon clock with experimental colitis through regulation of NF-?B/NLRP3 axis
    Authors: S Wang, Y Lin, X Yuan, F Li, L Guo, B Wu
    Nat Commun, 2018-10-12;9(1):4246.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  159. Cutting Edge: IL-1? and Not IL-1? Drives IL-1R1-Dependent Neonatal Murine Sepsis Lethality
    Authors: JT Benjamin, DJ Moore, C Bennett, R van der Me, A Royce, R Loveland, JL Wynn
    J. Immunol., 2018-10-10;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  160. Caspase-11-mediated tubular epithelial pyroptosis underlies contrast-induced acute kidney injury
    Authors: Z Zhang, X Shao, N Jiang, S Mou, L Gu, S Li, Q Lin, Y He, M Zhang, W Zhou, Z Ni
    Cell Death Dis, 2018-09-24;9(10):983.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  161. Interleukin-1? Maturation Triggers Its Relocation to the Plasma Membrane for Gasdermin-D-Dependent and -Independent Secretion
    Authors: M Monteleone, AC Stanley, KW Chen, DL Brown, JS Bezbradica, JB von Pein, CL Holley, D Boucher, MR Shakespear, R Kapetanovi, V Rolfes, MJ Sweet, JL Stow, K Schroder
    Cell Rep, 2018-08-07;24(6):1425-1433.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  162. NLRP3 inflammasome inhibitor OLT1177 suppresses joint inflammation in murine models of acute arthritis
    Authors: C Marchetti, B Swartzwelt, MI Koenders, T Azam, IW Tengesdal, N Powers, DM de Graaf, CA Dinarello, LAB Joosten
    Arthritis Res. Ther., 2018-08-03;20(1):169.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  163. NLRP3 Inflammasome Formation and Activation in Nonalcoholic Steatohepatitis: Therapeutic Target for Antimetabolic Syndrome Remedy FTZ
    Authors: Y Chen, X He, X Yuan, J Hong, O Bhat, G Li, PL Li, J Guo
    Oxid Med Cell Longev, 2018-07-22;2018(0):2901871.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  164. Menthol, a unique urinary volatile compound, is associated with chronic inflammation in interstitial cystitis
    Authors: M Shahid, MY Lee, A Yeon, E Cho, V Sairam, L Valdiviez, S You, J Kim
    Sci Rep, 2018-07-18;8(1):10859.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  165. Oridonin is a covalent NLRP3 inhibitor with strong anti-inflammasome activity
    Authors: H He, H Jiang, Y Chen, J Ye, A Wang, C Wang, Q Liu, G Liang, X Deng, W Jiang, R Zhou
    Nat Commun, 2018-06-29;9(1):2550.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  166. MPTP-driven NLRP3 inflammasome activation in microglia plays a central role in dopaminergic neurodegeneration
    Authors: E Lee, I Hwang, S Park, S Hong, B Hwang, Y Cho, J Son, JW Yu
    Cell Death Differ., 2018-05-21;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  167. Nonsaponin fraction of Korean Red Ginseng attenuates cytokine production via inhibition of TLR4 expression
    Authors: H Ahn, BC Han, J Kim, SG Kang, PH Kim, KH Jang, SH So, SH Lee, GS Lee
    J Ginseng Res, 2018-04-04;43(2):291-299.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  168. Tranilast directly targets NLRP3 to treat inflammasome-driven diseases
    Authors: Y Huang, H Jiang, Y Chen, X Wang, Y Yang, J Tao, X Deng, G Liang, H Zhang, W Jiang, R Zhou
    EMBO Mol Med, 2018-04-01;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  169. HMGB1/IL-1? complexes in plasma microvesicles modulate immune responses to burn injury
    Authors: LG Coleman, R Maile, SW Jones, BA Cairns, FT Crews
    PLoS ONE, 2018-03-30;13(3):e0195335.
    Species: Mouse
    Sample Types: Protein
    Applications: Western Blot
  170. Single-Cell RNA-Seq Reveals the Transcriptional Landscape and Heterogeneity of Aortic Macrophages in Murine Atherosclerosis
    Authors: C Cochain, E Vafadarnej, P Arampatzi, P Jaroslav, H Winkels, K Ley, D Wolf, AE Saliba, A Zernecke
    Circ. Res., 2018-03-15;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  171. The oxidized phospholipid oxPAPC protects from septic shock by targeting the non-canonical inflammasome in macrophages
    Authors: LH Chu, M Indramohan, RA Ratsimandr, A Gangopadhy, EP Morris, DM Monack, A Dorfleutne, C Stehlik
    Nat Commun, 2018-03-08;9(1):996.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  172. MCMV triggers ROS/NLRP3-associated inflammasome activation in the inner ear of mice and cultured spiral ganglion neurons, contributing to sensorineural hearing loss
    Authors: W Zhuang, C Wang, X Shi, S Qiu, S Zhang, B Xu, M Chen, W Jiang, H Dong, Y Qiao
    Int. J. Mol. Med., 2018-03-06;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  173. Artemisia Extract Suppresses NLRP3 and AIM2 Inflammasome Activation by Inhibition of ASC Phosphorylation
    Authors: SB Kwak, S Koppula, EJ In, X Sun, YK Kim, MK Kim, KH Lee, TB Kang
    Mediators Inflamm., 2018-03-04;2018(0):6054069.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  174. An unexpected link between fatty acid synthase and cholesterol synthesis in proinflammatory macrophage activation
    Authors: RG Carroll, Z Zas?ona, S Galván-Peñ, EL Koppe, DC Sévin, S Angiari, M Triantafil, K Triantafil, LK Modis, LA O'Neill
    J. Biol. Chem., 2018-02-20;293(15):5509-5521.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  175. LPS targets host guanylate-binding proteins to the bacterial outer membrane for non-canonical inflammasome activation
    Authors: JC Santos, MS Dick, B Lagrange, D Degrandi, K Pfeffer, M Yamamoto, E Meunier, P Pelczar, T Henry, P Broz
    EMBO J., 2018-02-19;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  176. Caspase-1 self-cleavage is an intrinsic mechanism to terminate inflammasome activity
    Authors: D Boucher, M Monteleone, RC Coll, KW Chen, CM Ross, JL Teo, GA Gomez, CL Holley, D Bierschenk, KJ Stacey, AS Yap, JS Bezbradica, K Schroder
    J. Exp. Med., 2018-02-06;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  177. Inhibitory Effect and Mechanism ofArctium lappaExtracton NLRP3Inflammasome Activation
    Authors: YK Kim, S Koppula, DW Shim, EJ In, SB Kwak, MK Kim, SH Yu, KH Lee, TB Kang
    Evid Based Complement Alternat Med, 2018-01-18;2018(0):6346734.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  178. Sex differences in primary muscle afferent sensitization following ischemia and reperfusion injury
    Authors: JL Ross, LF Queme, JE Lamb, KJ Green, MP Jankowski
    Biol Sex Differ, 2018-01-03;9(1):2.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  179. AKT1 distinctively suppresses MyD88-depenedent and TRIF-dependent Toll-like receptor signaling in a kinase activity-independent manner
    Authors: K Zenke, M Muroi, KI Tanamoto
    Cell. Signal., 2017-12-11;43(0):32-39.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  180. The PYHIN Protein p205 Regulates the Inflammasome by Controlling Asc Expression
    Authors: S Ghosh, C Wallerath, S Covarrubia, V Hornung, S Carpenter, KA Fitzgerald
    J. Immunol., 2017-09-20;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  181. Tetrahydrobiopterin (BH4) deficiency is associated with augmented inflammation and microvascular degeneration in the retina
    Authors: JC Rivera, B Noueihed, A Madaan, I Lahaie, J Pan, J Belik, S Chemtob
    J Neuroinflammation, 2017-09-06;14(1):181.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  182. MTG16 is a tumor suppressor in colitis-associated carcinoma
    Authors: EM McDonough, CW Barrett, B Parang, MK Mittal, JJ Smith, AM Bradley, YA Choksi, LA Coburn, SP Short, JJ Thompson, B Zhang, SV Poindexter, MA Fischer, X Chen, J Li, FL Revetta, R Naik, MK Washington, MJ Rosen, SW Hiebert, KT Wilson, CS Williams
    JCI Insight, 2017-08-17;2(16):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  183. The P2X7 Receptor Primes IL-1? and the NLRP3 Inflammasome in Astrocytes Exposed to Mechanical Strain
    Authors: F Albalawi, W Lu, JM Beckel, JC Lim, SA McCaughey, CH Mitchell
    Front Cell Neurosci, 2017-08-08;11(0):227.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  184. A new approach for ratiometric in vivo calcium imaging of microglia
    Authors: B Brawek, Y Liang, D Savitska, K Li, N Fomin-Thun, Y Kovalchuk, E Zirdum, J Jakobsson, O Garaschuk
    Sci Rep, 2017-07-20;7(1):6030.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  185. MLKL, the Protein that Mediates Necroptosis, Also Regulates Endosomal Trafficking and Extracellular Vesicle Generation
    Authors: S Yoon, A Kovalenko, K Bogdanov, D Wallach
    Immunity, 2017-06-27;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  186. Nucleoside reverse transcriptase inhibitors (NRTIs) induce proinflammatory cytokines in the CNS via Wnt5a signaling
    Authors: T Wu, J Zhang, M Geng, SJ Tang, W Zhang, J Shu
    Sci Rep, 2017-06-23;7(1):4117.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  187. The intracellular chloride channel proteins CLIC1 and CLIC4 induce IL-1? transcription and activate the NLRP3 inflammasome
    Authors: R Domingo-Fe, RC Coll, J Kearney, S Breit, LA O'Neill
    J. Biol. Chem., 2017-06-02;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  188. Myeloid-derived miR-223 regulates intestinal inflammation via repression of the NLRP3 inflammasome
    Authors: V Neudecker, M Haneklaus, O Jensen, L Khailova, JC Masterson, H Tye, K Biette, P Jedlicka, KS Brodsky, ME Gerich, M Mack, AAB Robertson, MA Cooper, GT Furuta, CA Dinarello, LA O'Neill, HK Eltzschig, SL Masters, EN McNamee
    J. Exp. Med., 2017-05-09;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  189. Cutting Edge: Distinct Regulatory Mechanisms Control Proinflammatory Cytokines IL-18 and IL-1?
    Authors: Q Zhu, TD Kanneganti
    J. Immunol., 2017-05-03;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  190. Lentinan from shiitake selectively attenuates AIM2 and non-canonical inflammasome activation while inducing pro-inflammatory cytokine production
    Authors: H Ahn, E Jeon, JC Kim, SG Kang, SI Yoon, HJ Ko, PH Kim, GS Lee
    Sci Rep, 2017-05-02;7(1):1314.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  191. IL-1? transcriptionally activates hepcidin by inducing C/EBP? expression in hepatocytes
    Authors: Y Kanamori, M Murakami, M Sugiyama, O Hashimoto, T Matsui, M Funaba
    J. Biol. Chem., 2017-04-24;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  192. NLR members NLRC4 and NLRP3 mediate sterile inflammasome activation in microglia and astrocytes
    Authors: L Freeman, H Guo, CN David, WJ Brickey, S Jha, JP Ting
    J. Exp. Med., 2017-04-12;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  193. Papilloma-pseudovirus eradicates intestinal tumours and triples the lifespan of Apc(Min/+) mice
    Authors: Z Zhong, Y Zhai, P Bu, S Shah, L Qiao
    Nat Commun, 2017-04-11;8(0):15004.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  194. TLR sensing of bacterial spore-associated RNA triggers host immune responses with detrimental effects
    Authors: MK Choo, Y Sano, C Kim, K Yasuda, XD Li, X Lin, M Stenzel-Po, L Alexopoulo, S Ghosh, E Latz, IR Rifkin, ZJ Chen, GC Stewart, H Chong, JM Park
    J. Exp. Med., 2017-04-11;214(5):1297-1311.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  195. Distinct Kinase-Independent Role of RIPK3 in CD11c(+) Mononuclear Phagocytes in Cytokine-Induced Tissue Repair
    Authors: K Moriwaki, S Balaji, J Bertin, PJ Gough, FK Chan
    Cell Rep, 2017-03-07;18(10):2441-2451.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  196. Interleukin-1 and estrogen protect against disseminating dentoalveolar infections
    Authors: H Youssef, P Stashenko
    Int J Oral Sci, 2017-03-01;9(1):16-23.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  197. Quercetin Inhibits Inflammasome Activation by Interfering with ASC Oligomerization and Prevents Interleukin-1 Mediated Mouse Vasculitis
    Authors: TP Domiciano, D Wakita, HD Jones, TR Crother, WA Verri, M Arditi, K Shimada
    Sci Rep, 2017-02-02;7(0):41539.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  198. SR-B1 Is a Silica Receptor that Mediates Canonical Inflammasome Activation
    Authors: M Tsugita, N Morimoto, M Tashiro, K Kinoshita, M Nakayama
    Cell Rep, 2017-01-31;18(5):1298-1311.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  199. Targeting IRE1 with small molecules counteracts progression of atherosclerosis
    Authors: O Tufanli, P Telkoparan, D Acosta-Alv, B Kocaturk, UI Onat, SM Hamid, I €imen, P Walter, C Weber, E Erbay
    Proc. Natl. Acad. Sci. U.S.A, 2017-01-30;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  200. Toxoplasma gondii GRA7-Targeted ASC and PLD1 Promote Antibacterial Host Defense via PKC�
    Authors: HJ Koh, YR Kim, JS Kim, JS Yun, K Jang, CS Yang
    PLoS Pathog, 2017-01-26;13(1):e1006126.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  201. Histone deacetylase inhibitors mediate DNA damage repair in ameliorating hemorrhagic cystitis
    Sci Rep, 2016-12-20;6(0):39257.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  202. Functional and Evolutionary Analyses Identify Proteolysis as a General Mechanism for NLRP1 Inflammasome Activation
    PLoS Pathog, 2016-12-07;12(12):e1006052.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  203. TNF?-stimulated gene-6 (TSG6) activates macrophage phenotype transition to prevent inflammatory lung injury
    Proc. Natl. Acad. Sci. U.S.A., 2016-11-28;113(50):E8151-E8158.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  204. Pericyte MyD88 and IRAK4 control inflammatory and fibrotic responses to tissue injury
    Authors: Jeremy S Duffield
    J. Clin. Invest., 2016-11-21;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  205. CARD9 negatively regulates NLRP3-induced IL-1? production on Salmonella infection of macrophages
    Nat Commun, 2016-09-27;7(0):12874.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  206. Fluoxetine Inhibits NLRP3 Inflammasome Activation: Implication in Depression
    Authors: Gang Hu
    Int. J. Neuropsychopharmacol., 2016-09-21;19(9):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  207. Lymphatic vessels regulate immune microenvironments in human and murine melanoma
    J Clin Invest, 2016-08-15;0(0):.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  208. CD8+ T cells produce a dialyzable antigen-specific activator of dendritic cells
    J Leukoc Biol, 2016-08-11;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  209. C13orf31 (FAMIN) is a central regulator of immunometabolic function
    Nat Immunol, 2016-08-01;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  210. HIF-1? Is an Essential Mediator of IFN-?-Dependent Immunity to Mycobacterium tuberculosis
    J Immunol, 2016-07-18;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  211. Enzymatically inactive procaspase-1 stabilizes the ASC-pyroptosome and supports pyroptosome spreading during cell division
    J Biol Chem, 2016-07-08;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Bead-based Immunoassay
  212. Activation of NLRP3 inflammasomes in mouse hepatic stellate cells during Schistosoma J. infection
    Oncotarget, 2016-06-28;7(26):39316-39331.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC, IHC-P
  213. Pyrin inflammasome activation and RhoA signaling in the autoinflammatory diseases FMF and HIDS
    Authors: Yong Hwan Park
    Nat Immunol, 2016-06-06;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  214. The Tick Protein Sialostatin L2 Binds to Annexin A2 and Inhibits NLRC4-Mediated Inflammasome Activation
    Authors: X Wang, DK Shaw, OS Sakhon, GA Snyder, EJ Sundberg, L Santambrog, FS Sutterwala, JS Dumler, KA Shirey, DJ Perkins, K Richard, AC Chagas, E Calvo, J Kopecký, M Kotsyfakis, JH Pedra
    Infect Immun, 2016-05-24;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  215. Identification of QS-21 as an Inflammasome-activating Molecular Component of Saponin Adjuvants.
    Authors: Marty-Roix R, Vladimer G, Pouliot K, Weng D, Buglione-Corbett R, West K, Macmicking J, Chee J, Wang S, Lu S, Lien E
    J Biol Chem, 2015-11-10;291(3):1123-36.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  216. Deficient NLRP3 and AIM2 Inflammasome Function in Autoimmune NZB Mice.
    Authors: Sester D, Sagulenko V, Thygesen S, Cridland J, Loi Y, Cridland S, Masters S, Genske U, Hornung V, Andoniou C, Sweet M, Degli-Esposti M, Schroder K, Stacey K
    J Immunol, 2015-06-26;195(3):1233-41.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  217. Caspase-1-independent IL-1 release mediates blister formation in autoantibody-induced tissue injury through modulation of endothelial adhesion molecules.
    Authors: Sadeghi H, Lockmann A, Hund A, Samavedam U, Pipi E, Vafia K, Hauenschild E, Kalies K, Pas H, Jonkman M, Iwata H, Recke A, Schon M, Zillikens D, Schmidt E, Ludwig R
    J Immunol, 2015-03-20;194(8):3656-63.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  218. Guanylate-binding proteins promote activation of the AIM2 inflammasome during infection with Francisella novicida.
    Authors: Meunier E, Wallet P, Dreier R, Costanzo S, Anton L, Ruhl S, Dussurgey S, Dick M, Kistner A, Rigard M, Degrandi D, Pfeffer K, Yamamoto M, Henry T, Broz P
    2015-03-16;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  219. AIM2 and NLRC4 inflammasomes contribute with ASC to acute brain injury independently of NLRP3.
    Authors: Denes A, Coutts G, Lenart N, Cruickshank S, Pelegrin P, Skinner J, Rothwell N, Allan S, Brough D
    Proc Natl Acad Sci U S A, 2015-03-16;112(13):4050-5.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  220. Local immunosuppressive microenvironment enhances migration of melanoma cells to lungs in DJ-1 knockout mice.
    Authors: Chien C, Lee M, Liou H, Liou H, Fu W
    PLoS ONE, 2015-02-23;10(2):e0115827.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  221. RIPK3 promotes cell death and NLRP3 inflammasome activation in the absence of MLKL.
    Authors: Lawlor K, Khan N, Mildenhall A, Gerlic M, Croker B, D'Cruz A, Hall C, Kaur Spall S, Anderton H, Masters S, Rashidi M, Wicks I, Alexander W, Mitsuuchi Y, Benetatos C, Condon S, Wong W, Silke J, Vaux D, Vince J
    Nat Commun, 2015-02-18;6(0):6282.
    Species: Mouse
    Sample Types: Cell Lysates, In Vivo
    Applications: Neutralization, Western Blot
  222. The cyclopentenone prostaglandin 15d-PGJ2 inhibits the NLRP1 and NLRP3 inflammasomes.
    Authors: Maier N, Leppla S, Moayeri M
    J Immunol, 2015-02-13;194(6):2776-85.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  223. Small heterodimer partner interacts with NLRP3 and negatively regulates activation of the NLRP3 inflammasome.
    Authors: Yang C, Kim J, Kim T, Lee P, Kim S, Lee H, Shin D, Nguyen L, Lee M, Jin H, Kim K, Lee C, Kim M, Park S, Kim J, Choi H, Jo E
    Nat Commun, 2015-02-06;6(0):6115.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  224. IL-10 engages macrophages to shift Th17 cytokine dependency and pathogenicity during T-cell-mediated colitis.
    Authors: Li B, Gurung P, Malireddi R, Vogel P, Kanneganti T, Geiger T
    Nat Commun, 2015-01-21;6(0):6131.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  225. A RIPK3-caspase 8 complex mediates atypical pro-IL-1beta processing.
    Authors: Moriwaki K, Bertin J, Gough P, Chan F
    J Immunol, 2015-01-07;194(4):1938-44.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  226. Retinal neurons curb inflammation and enhance revascularization in ischemic retinopathies via proteinase-activated receptor-2.
    Authors: Sitaras N, Rivera J, Noueihed B, Bien-Aime M, Zaniolo K, Omri S, Hamel D, Zhu T, Hardy P, Sapieha P, Joyal J, Chemtob S
    Am J Pathol, 2014-12-03;185(2):581-95.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  227. Activation of NLRP3 inflammasome by crystalline structures via cell surface contact.
    Authors: Hari A, Zhang Y, Tu Z, Detampel P, Stenner M, Ganguly A, Shi Y
    Sci Rep, 2014-12-02;4(0):7281.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  228. Methylsulfonylmethane inhibits NLRP3 inflammasome activation.
    Authors: Ahn H, Kim J, Lee M, Kim Y, Cho Y, Lee G
    Cytokine, 2014-11-21;71(2):223-31.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  229. Systemic autophagy insufficiency compromises adaptation to metabolic stress and facilitates progression from obesity to diabetes.
    Authors: Lim, Yu-Mi, Lim, Hyejin, Hur, Kyu Yeon, Quan, Wenying, Lee, Hae-Youn, Cheon, Hwanju, Ryu, Dongryeo, Koo, Seung-Ho, Kim, Hong Lim, Kim, Jin, Komatsu, Masaaki, Lee, Myung-Sh
    Nat Commun, 2014-09-26;5(0):4934.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  230. Caspase-8 modulates dectin-1 and complement receptor 3-driven IL-1beta production in response to beta-glucans and the fungal pathogen, Candida albicans.
    Authors: Ganesan S, Rathinam V, Bossaller L, Army K, Kaiser W, Mocarski E, Dillon C, Green D, Mayadas T, Levitz S, Hise A, Silverman N, Fitzgerald K
    J Immunol, 2014-07-25;193(5):2519-30.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  231. The frustrated host response to Legionella pneumophila is bypassed by MyD88-dependent translation of pro-inflammatory cytokines.
    Authors: Asrat S, Dugan A, Isberg R
    PLoS Pathog, 2014-07-24;10(7):e1004229.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  232. A critical role for human caspase-4 in endotoxin sensitivity.
    Authors: Kajiwara Y, Schiff T, Voloudakis G, Gama Sosa M, Elder G, Bozdagi O, Buxbaum J
    J Immunol, 2014-05-30;193(1):335-43.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  233. Nuclear factor E2-related factor-2 (Nrf2) is required for NLRP3 and AIM2 inflammasome activation.
    Authors: Zhao C, Gillette D, Li X, Zhang Z, Wen H
    J Biol Chem, 2014-05-05;289(24):17020-9.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  234. Severe acute respiratory syndrome coronavirus envelope protein ion channel activity promotes virus fitness and pathogenesis.
    Authors: Nieto-Torres J, DeDiego M, Verdia-Baguena C, Jimenez-Guardeno J, Regla-Nava J, Fernandez-Delgado R, Castano-Rodriguez C, Alcaraz A, Torres J, Aguilella V, Enjuanes L
    PLoS Pathog, 2014-05-01;10(5):e1004077.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  235. Caspase-11 activation requires lysis of pathogen-containing vacuoles by IFN-induced GTPases.
    Authors: Meunier E, Dick M, Dreier R, Schurmann N, Kenzelmann Broz D, Warming S, Roose-Girma M, Bumann D, Kayagaki N, Takeda K, Yamamoto M, Broz P
    Nature, 2014-04-16;509(7500):366-70.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  236. Critical role of aquaporins in interleukin 1beta (IL-1beta)-induced inflammation.
    Authors: Rabolli V, Wallemme L, Lo Re S, Uwambayinema F, Palmai-Pallag M, Thomassen L, Tyteca D, Octave J, Marbaix E, Lison D, Devuyst O, Huaux F
    J Biol Chem, 2014-04-03;289(20):13937-47.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  237. RNA and beta-hemolysin of group B Streptococcus induce interleukin-1beta (IL-1beta) by activating NLRP3 inflammasomes in mouse macrophages.
    Authors: Gupta R, Ghosh S, Monks B, DeOliveira R, Tzeng T, Kalantari P, Nandy A, Bhattacharjee B, Chan J, Ferreira F, Rathinam V, Sharma S, Lien E, Silverman N, Fitzgerald K, Firon A, Trieu-Cuot P, Henneke P, Golenbock D
    J Biol Chem, 2014-04-01;289(20):13701-5.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  238. Endothelial NLRP3 inflammasome activation and enhanced neointima formation in mice by adipokine visfatin.
    Authors: Xia M, Boini K, Abais J, Xu M, Zhang Y, Li P
    Am J Pathol, 2014-03-13;184(5):1617-28.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  239. Critical role for IL-1beta in DNA damage-induced mucositis.
    Authors: Kanarek N, Grivennikov S, Leshets M, Lasry A, Alkalay I, Horwitz E, Shaul Y, Stachler M, Voronov E, Apte R, Pagano M, Pikarsky E, Karin M, Ghosh S, Ben-Neriah Y
    Proc Natl Acad Sci U S A, 2014-01-27;111(6):E702-11.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  240. Arsenic trioxide and other arsenical compounds inhibit the NLRP1, NLRP3, and NAIP5/NLRC4 inflammasomes.
    Authors: Maier N, Crown D, Liu J, Leppla S, Moayeri M
    J Immunol, 2013-12-13;192(2):763-70.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  241. Activation of microglia induces symptoms of Parkinson's disease in wild-type, but not in IL-1 knockout mice.
    Authors: Tanaka S, Ishii A, Ohtaki H, Shioda S, Yoshida T, Numazawa S
    J Neuroinflammation, 2013-12-01;10(0):143.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  242. 3,4-methylenedioxy-beta-nitrostyrene inhibits NLRP3 inflammasome activation by blocking assembly of the inflammasome.
    Authors: He Y, Varadarajan S, Munoz-Planillo R, Burberry A, Nakamura Y, Nunez G
    J Biol Chem, 2013-11-21;289(2):1142-50.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  243. Signaling pathways in murine dendritic cells that regulate the response to vesicular stomatitis virus vectors that express flagellin.
    Authors: Smedberg J, Westcott M, Ahmed M, Lyles D
    J Virol, 2013-11-06;88(2):777-85.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  244. Wingless-type mammary tumor virus integration site family, member 5A (Wnt5a) regulates human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein 120 (gp120)-induced expression of pro-inflammatory cytokines via the Ca2+/calmodulin-dependent protein kinase II (CaMKII) and c-Jun N-terminal kinase (JNK) signaling pathways.
    Authors: Li B, Shi Y, Shu J, Gao J, Wu P, Tang S
    J Biol Chem, 2013-03-28;288(19):13610-9.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  245. Microtubule-driven spatial arrangement of mitochondria promotes activation of the NLRP3 inflammasome.
    Authors: Misawa T, Takahama M, Kozaki T, Lee H, Zou J, Saitoh T, Akira S
    Nat Immunol, 2013-03-17;14(5):454-60.
    Species: Mouse
    Sample Types: Peritoneal Fluid
    Applications: Western Blot
  246. The role of IL-1beta in the early tumor cell-induced angiogenic response.
    Authors: Carmi Y, Dotan S, Rider P, Kaplanov I, White M, Baron R, Abutbul S, Huszar M, Dinarello C, Apte R, Voronov E
    J Immunol, 2013-03-08;190(7):3500-9.
    Species: Mouse
    Sample Types: In Vivo, Matrigel Plug
    Applications: IHC-Fr, IHC-P, Neutralization
  247. Gli1 deletion prevents Helicobacter-induced gastric metaplasia and expansion of myeloid cell subsets.
    Authors: El-Zaatari M, Kao J, Tessier A, Bai L, Hayes M, Fontaine C, Eaton K, Merchant J
    PLoS ONE, 2013-03-08;8(3):e58935.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  248. The calcium-sensing receptor regulates the NLRP3 inflammasome through Ca2+ and cAMP.
    Authors: Lee G, Subramanian N, Kim A, Aksentijevich I, Goldbach-Mansky R, Sacks D, Germain R, Kastner D, Chae J
    Nature, 2012-11-11;492(7427):123-7.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  249. Caspase-11 increases susceptibility to Salmonella infection in the absence of caspase-1.
    Authors: Broz P, Ruby T, Belhocine K, Bouley D, Kayagaki N, Dixit V, Monack D
    Nature, 2012-08-15;490(7419):288-91.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  250. Dectin-1-dependent interleukin-22 contributes to early innate lung defense against Aspergillus fumigatus.
    Authors: Gessner MA, Werner JL, Lilly LM, Nelson MP, Metz AE, Dunaway CW, Chan YR, Ouyang W, Brown GD, Weaver CT, Steele C
    Infect. Immun., 2011-10-28;80(1):410-7.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  251. Toll-like receptor 2 contributes to chemokine gene expression and macrophage infiltration in the dorsal root ganglia after peripheral nerve injury.
    Authors: Kim D, You B, Lim H, Lee SJ
    Mol Pain, 2011-09-28;7(0):74.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  252. IL-6 trans-signaling licenses mouse and human tumor microvascular gateways for trafficking of cytotoxic T cells.
    Authors: Fisher DT, Chen Q, Skitzki JJ, Muhitch JB, Zhou L, Appenheimer MM, Vardam TD, Weis EL, Passanese J, Wang WC, Gollnick SO, Dewhirst MW, Rose-John S, Repasky EA, Baumann H, Evans SS
    J. Clin. Invest., 2011-09-19;121(10):3846-59.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  253. IL-1alpha and IL-1beta recruit different myeloid cells and promote different stages of sterile inflammation.
    Authors: Rider P, Carmi Y, Guttman O, Braiman A, Cohen I, Voronov E, White MR, Dinarello CA, Apte RN
    J. Immunol., 2011-09-19;187(9):4835-43.
    Species: Mouse
    Sample Types: In Vivo, Whole Cells, Whole Tissue
    Applications: ICC, IHC-Fr, Neutralization
  254. Coordinated Host Responses during Pyroptosis: Caspase-1-Dependent Lysosome Exocytosis and Inflammatory Cytokine Maturation.
    Authors: Bergsbaken T, Fink SL, den Hartigh AB, Loomis WP, Cookson BT
    J. Immunol., 2011-07-29;187(5):2748-54.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  255. Microenvironment-derived IL-1 and IL-17 interact in the control of lung metastasis.
    Authors: Carmi Y, Rinott G, Dotan S, Elkabets M, Rider P, Voronov E, Apte RN
    J. Immunol., 2011-02-07;186(6):3462-71.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  256. Interleukin-1beta mediates the extra-intestinal thrombosis associated with experimental colitis.
    Authors: Yoshida H, Russell J, Senchenkova EY, Almeida Paula LD, Granger DN
    Am. J. Pathol., 2010-10-22;177(6):2774-81.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  257. Cytotoxins of the human pathogen Aeromonas hydrophila trigger, via the NLRP3 inflammasome, caspase-1 activation in macrophages.
    Authors: McCoy AJ, Koizumi Y, Toma C, Higa N, Dixit V, Taniguchi S, Tschopp J, Suzuki T
    Eur. J. Immunol., 2010-10-01;40(10):2797-803.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  258. Activation of the NLRP3 inflammasome by islet amyloid polypeptide provides a mechanism for enhanced IL-1beta in type 2 diabetes.
    Authors: Masters SL, Dunne A, Subramanian SL, Hull RL, Tannahill GM, Sharp FA, Becker C, Franchi L, Yoshihara E, Chen Z, Mullooly N, Mielke LA, Harris J, Coll RC, Mills KH, Mok KH, Newsholme P, Nunez G, Yodoi J, Kahn SE, Lavelle EC, O'Neill LA
    Nat. Immunol., 2010-09-12;11(10):897-904.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  259. Tyrosine kinase 2 controls IL-1beta production at the translational level.
    Authors: Radwan M, Stiefvater R, Grunert T, Sharif O, Miller I, Marchetti-Deschmann M, Allmaier G, Gemeiner M, Knapp S, Kovarik P, Muller M, Strobl B
    J. Immunol., 2010-08-16;185(6):3544-53.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  260. Caspase-1, caspase-8, and calpain are dispensable for IL-33 release by macrophages.
    Authors: Ohno T, Oboki K, Kajiwara N, Morii E, Aozasa K, Flavell RA, Okumura K, Saito H, Nakae S
    J. Immunol., 2009-12-15;183(12):7890-7.
    Species: Mouse
    Sample Types: DNA
    Applications: MUSTag PCR Assay
  261. Activation of the NLRP3 inflammasome in dendritic cells induces IL-1beta-dependent adaptive immunity against tumors.
    Authors: Ghiringhelli F, Apetoh L, Tesniere A, Aymeric L, Ma Y, Ortiz C, Vermaelen K, Panaretakis T, Mignot G, Ullrich E, Perfettini JL, Schlemmer F, Tasdemir E, Uhl M, Genin P, Civas A, Ryffel B, Kanellopoulos J, Tschopp J, Andre F, Lidereau R, McLaughlin NM, Haynes NM, Smyth MJ, Kroemer G, Zitvogel L
    Nat. Med., 2009-09-20;15(10):1170-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  262. Externalization of the leaderless cytokine IL-1F6 occurs in response to lipopolysaccharide/ATP activation of transduced bone marrow macrophages.
    Authors: Martin U, Scholler J, Gurgel J, Renshaw B, Sims JE, Gabel CA
    J. Immunol., 2009-08-28;183(6):4021-30.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  263. Biglycan, a danger signal that activates the NLRP3 inflammasome via toll-like and P2X receptors.
    Authors: Babelova A, Moreth K, Tsalastra-Greul W, Zeng-Brouwers J, Eickelberg O, Young MF, Bruckner P, Pfeilschifter J, Schaefer RM, Schaefer R, Grone HJ, Schaefer L
    J Biol Chem, 2009-07-15;284(36):24035-48.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  264. Chlamydial and periodontal pathogens induce hepatic inflammation and fatty acid imbalance in apolipoprotein E-deficient mice.
    Authors: Hyvarinen K, Tuomainen AM, Laitinen S, Bykov IL, Tormakangas L, Lindros K, Käkelä R, Alfthan G, Salminen I, Jauhiainen M, Kovanen PT, Leinonen M, Saikku P, Pussinen PJ
    Infect. Immun., 2009-05-18;77(8):3442-9.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  265. Stimulation of Toll-like receptor 3 and 4 induces interleukin-1beta maturation by caspase-8.
    Authors: Maelfait J, Vercammen E, Janssens S, Schotte P, Haegman M, Magez S, Beyaert R
    J. Exp. Med., 2008-08-25;205(9):1967-73.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  266. Japanese Encephalitis Virus infection induces IL-18 and IL-1beta in microglia and astrocytes: correlation with in vitro cytokine responsiveness of glial cells and subsequent neuronal death.
    Authors: Das S, Mishra MK, Ghosh J, Basu A
    J. Neuroimmunol., 2008-03-28;195(1):60-72.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-Fr, Western Blot
  267. Inflammasome-mediated production of IL-1beta is required for neutrophil recruitment against Staphylococcus aureus in vivo.
    Authors: Miller LS, Pietras EM, Uricchio LH, Hirano K, Rao S, Lin H, O'Connell RM, Iwakura Y, Cheung AL, Cheng G, Modlin RL
    J. Immunol., 2007-11-15;179(10):6933-42.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  268. Topical acetone treatment induces neurogenic oedema on the sensitized mouse ear: an in vivo study using transient receptor potential vanilloid 1 (TRPV1) receptor knockout mice.
    Authors: Pozsgai G, Sandor K, Perkecz A, Szolcsanyi J, Helyes Z, Brain SD, Pinter E
    Inflamm. Res., 2007-11-01;56(11):459-67.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  269. Granulocyte colony-stimulating factor impairs liver regeneration in mice through the up-regulation of interleukin-1beta.
    Authors: Ogiso T, Nagaki M, Takai S, Tsukada Y, Mukai T, Kimura K, Moriwaki H
    J. Hepatol., 2007-08-10;47(6):816-25.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  270. Toll-like receptor 4 (TLR4)-dependent proinflammatory and immunomodulatory properties of the glycoinositolphospholipid (GIPL) from Trypanosoma cruzi.
    Authors: Medeiros MM, Peixoto JR, Oliveira AC, Cardilo-Reis L, Koatz VL, Van Kaer L, Previato JO, Mendonca-Previato L, Nobrega A, Bellio M
    J. Leukoc. Biol., 2007-05-31;82(3):488-96.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  271. The Myc-dependent angiogenic switch in tumors is mediated by interleukin 1beta.
    Authors: Shchors K, Shchors E, Rostker F, Lawlor ER, Brown-Swigart L, Evan GI
    Genes Dev., 2006-09-15;20(18):2527-38.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  272. Involvement of monocyte chemoattractant protein-1, macrophage inflammatory protein-1alpha and interleukin-1beta in Wallerian degeneration.
    Authors: Perrin FE, Lacroix S, Aviles-Trigueros M, David S
    Brain, 2005-02-02;128(0):854-66.
    Species: Mouse
    Sample Types:
    Applications: Neutralization
  273. Intravenous human interleukin-1alpha impairs memory processing in mice: dependence on blood-brain barrier transport into posterior division of the septum.
    Authors: Banks WA, Farr SA, La Scola ME
    J. Pharmacol. Exp. Ther., 2001-11-01;299(2):536-41.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization

FAQs

  1. Does catalog # AF-401-NA detect the pro or mature form of IL-1 beta in western blot?

    • This would depend on the sample being used. The pro-form of the protein also contains the sequence for the mature form.  When cell lysates are used as the sample, the pro-form is mainly detected.  When cell culture supernatants containing mainly the mature form of the protein is used, only the mature form is detected.  This is exemplified in the following paper:

      J Immunol.(2015) 195, 1233.  http://www.jimmunol.org/content/195/3/1233/tab-figures-data

  2. Does Mouse IL-1 beta /IL-1F2 Antibody, Catalog # AF-401-NA, detect cleaved IL-1beta (17kDa)?

    • The immunogen that was used to produce Catalog # AF-401-NA consists of the mature form of IL-1beta, amino acids Val118-Ser269. Since the pro-form also includes this sequence, we expect this antibody to detect the pro-form and cleaved IL-1beta.

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Reviews for Mouse IL-1 beta /IL-1F2 Antibody

Average Rating: 4 (Based on 21 Reviews)

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Mouse IL-1 beta /IL-1F2 Antibody
By Anonymous on 07/25/2023
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Aorta tissue Species: Mouse

Mouse IL-1 beta /IL-1F2 Antibody
By Katie Myers on 11/29/2022
Application: ELISA Sample Tested: Serum Species: Mouse

Used as ELISA detection


Mouse IL-1 beta /IL-1F2 Antibody
By Anonymous on 11/08/2022
Application: WB Sample Tested: Lysates of primary bone marrow derived macrophage Species: Mouse

IL-1beta band comes at 32 KDa for the pro-IL-1b.


Mouse IL-1 beta /IL-1F2 Antibody
By Anonymous on 08/28/2022
Application: Flow Sample Tested: Leukocyte Species: Mouse

Works well with flow cytometry!


Mouse IL-1 beta /IL-1F2 Antibody
By Anonymous on 12/14/2021
Application: Flow Sample Tested: Peripheral blood mononuclear cells (PBMCs) Species: Human

PBMCs either left resting or stimulated with LPS (1ug, 3h). Addition of GolgiPlug to detect intracellular IL1B production. Use of a secondary anti-goat antibody, conjugated to AF488.


Mouse IL-1 beta /IL-1F2 Antibody
By Tina Yuan on 11/23/2020
Application: IHC Sample Tested: mouse oral tissue Species: Mouse

Mouse IL-1 beta /IL-1F2 Antibody
By Anonymous on 11/19/2020
Application: WB Sample Tested: THP-1 human acute monocytic leukemia cell line Species: Human

Human-derived THP-1 cells either left unstimulated or treated with LPS+ATP for 3 hours. Intracellular detection.


Mouse IL-1 beta /IL-1F2 Antibody
By Miguel Lopez Cuina on 05/23/2019
Application: WB Sample Tested: Brain (caudate putamen) Species: Mouse

I am somewhat disappointed. My lab published 4 years ago an article using this Ab. I boight it last september.
There is no way I can get the bands ourselves and other authors have published: the proIL1-beta and the mature form, at around 35 and 17kDa.
Attached is a representative blot of the many I have run.
I have tried homemade 12% gels, BioRad 12 and 4-20% precast gels. I have tried both types of membranes (PVDF and Nitro 0.2), blocking with milk and BSA, different transfer times (35min 120V, 45min 100V, 1h 100V, 1.5h 100V), different Ab dilutions. And I still get nothing that comes to close to the pro-IL-1beta, and of course, the mature form is nowhere to be found.
I do not wish to buy another brand, I believe this to be a good Ab, but since it is polyclonal, it might give different results depending on the lot. ***Bio-Techne Response: Thank you for reviewing our product. We are sorry to hear that this antibody did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.***


Mouse IL-1 beta /IL-1F2 Antibody
By Anonymous on 01/28/2019
Application: WB Sample Tested: IC-21 mouse macrophage cell line Species: Mouse

Mouse IL-1 beta /IL-1F2 Antibody
By Anthony Morales on 01/28/2019
Application: WB Sample Tested: IC-21 mouse macrophage cell line Species: Mouse

Mouse IL-1 beta /IL-1F2 Antibody
By CARLA RAMON on 08/08/2018
Application: IHC Sample Tested: Brain tissue Species: Mouse

10.1093/cercor/bhy164


Mouse IL-1 beta /IL-1F2 Antibody
By Anonymous on 07/17/2018
Application: Simple Western Sample Tested: J774A.1 mouse reticulum cell sarcoma macrophage cell line Species: Mouse

Mouse IL-1 beta /IL-1F2 Antibody
By Paloma Narros on 07/17/2018
Application: WB Sample Tested: Cell Lysates,Macrophages Cell Supernatant Species: Mouse and Mouse macrophages

Mouse IL-1 beta /IL-1F2 Antibody
By Anonymous on 07/17/2018
Application: WB Sample Tested: Cell Lysates Species: Mouse

Mouse IL-1 beta /IL-1F2 Antibody
By Alisha Freeman on 05/17/2018
Application: ELISA Sample Tested: A549 human lung carcinoma cell line Species: Human

Mouse IL-1 beta /IL-1F2 Antibody
By Rupal Soder on 03/22/2018
Application: WB Sample Tested: IC-21 mouse macrophage cell line Species: Mouse

Mouse IL-1 beta /IL-1F2 Antibody
By Anonymous on 10/31/2017
Application: WB Sample Tested: bone marrow derived macrophage Species: Mouse

Mouse IL-1 beta /IL-1F2 Antibody
By Anonymous on 09/08/2017
Application: Flow Sample Tested: L-929 mouse fibroblast cell line Species: Mouse

Mouse IL-1 beta /IL-1F2 Antibody
By Michelle Chen on 04/26/2017
Application: B/N Sample Tested: Adult splenocytes Species: Mouse

Mouse IL-1 beta /IL-1F2 Antibody
By Goutham Pattabiraman on 04/25/2017
Application: WB Sample Tested: Bone marrow cells,Cell culture supernatant,Serum-free Cell Culture Supernates Species: Mouse

Mouse IL-1 beta /IL-1F2 Antibody
By Anonymous on 02/14/2017
Application: WB Sample Tested: bone marrow derived macrophages Species: Mouse