Mouse IL-1 beta /IL-1F2 Antibody

  (95 citations)
(7 Reviews)
    
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Citations (95)
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  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse IL-1 beta /IL-1F2 in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant rat IL‑1 beta and recombinant porcine IL-1 beta is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant mouse IL-1 beta /IL-1F2
    Val118-Ser269
    Accession # NP_032387
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.25 µg/mL
    See below
  • Simple Western
    2.5 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Immunocytochemistry
    5-15 µg/mL
    See below
  • Neutralization
    Measured by its ability to neutralize IL‑1 beta /IL‑1F2-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND50) is typically ≤0.25 µg/mL in the presence of 50 pg/mL Recombinant Mouse IL‑1 beta /IL‑1F2.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
IL‑1 beta /IL‑1F2 in MCF‑7 Human Cell Line. IL‑1 beta /IL‑1F2 was detected in immersion fixed MCF‑7 human breast cancer cell line using Goat Anti-Mouse IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-401-NA) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human and Mouse
IL‑1 beta /IL‑1F2 by Western Blot.
Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line untreated (-)ortreated (+) with 200 nM PMA for 24 hoursand 10 μg/mL LPS for 4 hours andRAW 264.7 mouse monocyte/macrophagecell line untreated (-)or treated (+) with10 μg/mL LPS for 24 hours. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-401-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL‑1 beta /IL‑1F2 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
IL‑1 beta /IL‑1F2 in Mouse Thymus. IL‑1 beta /IL‑1F2 was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-401-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Detection of Mouse IL‑1 beta /IL‑1F2 by Simple WesternTM. Simple Western lane view shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with 10 µg/mL LPS for 24 hours, loaded at 0.5 mg/mL. A specific band was detected for IL‑1 beta /IL‑1F2 at approximately 40 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Mouse IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-401-NA) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.
Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutralization by Mouse
IL-1 beta /IL-1F2 Antibody.
Recombinant Mouse
IL‑1 beta /IL‑1F2 (Catalog # 401-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Mouse IL‑1 beta /IL‑1F2 (50 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-1 beta /IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-401-NA). The ND50 is typically ≤0.25 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-1 beta/IL-1F2
IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 17% amino acid (aa) identity in mouse. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. The mouse IL-1 beta cDNA encodes a 269 aa precursor. A 117 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine. The 17 kDa mature mouse IL-1 beta shares 90% aa sequence identity with cotton rat and rat and 65-78% identity with canine, equine, feline, human, porcine, and rhesus macaque IL-1 beta.
  • Long Name:
    Interleukin 1 beta
  • Entrez Gene IDs:
    3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
  • Alternate Names:
    catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

95 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. An unexpected link between fatty acid synthase and cholesterol synthesis in proinflammatory macrophage activation
    Authors: RG Carroll, Z Zas?ona, S Galván-Peñ, EL Koppe, DC Sévin, S Angiari, M Triantafil, K Triantafil, LK Modis, LA O'Neill
    J. Biol. Chem., 2018;293(15):5509-5521.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: WB
  2. Artemisia Extract Suppresses NLRP3 and AIM2 Inflammasome Activation by Inhibition of ASC Phosphorylation
    Authors: SB Kwak, S Koppula, EJ In, X Sun, YK Kim, MK Kim, KH Lee, TB Kang
    Mediators Inflamm., 2018;2018(0):6054069.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: WB
  3. HMGB1/IL-1? complexes in plasma microvesicles modulate immune responses to burn injury
    Authors: LG Coleman, R Maile, SW Jones, BA Cairns, FT Crews
    PLoS ONE, 2018;13(3):e0195335.
    Species: Mouse
    Sample Type: Protein
    Application: WB
  4. The oxidized phospholipid oxPAPC protects from septic shock by targeting the non-canonical inflammasome in macrophages
    Authors: LH Chu, M Indramohan, RA Ratsimandr, A Gangopadhy, EP Morris, DM Monack, A Dorfleutne, C Stehlik
    Nat Commun, 2018;9(1):996.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  5. Tranilast directly targets NLRP3 to treat inflammasome-driven diseases
    Authors: Y Huang, H Jiang, Y Chen, X Wang, Y Yang, J Tao, X Deng, G Liang, H Zhang, W Jiang, R Zhou
    EMBO Mol Med, 2018;0(0):.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  6. Inhibitory Effect and Mechanism ofArctium lappaExtracton NLRP3Inflammasome Activation
    Authors: YK Kim, S Koppula, DW Shim, EJ In, SB Kwak, MK Kim, SH Yu, KH Lee, TB Kang
    Evid Based Complement Alternat Med, 2018;2018(0):6346734.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  7. Cutting Edge: Distinct Regulatory Mechanisms Control Proinflammatory Cytokines IL-18 and IL-1?
    Authors: Q Zhu, TD Kanneganti
    J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  8. Toxoplasma gondii GRA7-Targeted ASC and PLD1 Promote Antibacterial Host Defense via PKC�
    Authors: HJ Koh, YR Kim, JS Kim, JS Yun, K Jang, CS Yang
    PLoS Pathog, 2017;13(1):e1006126.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  9. Targeting IRE1 with small molecules counteracts progression of atherosclerosis
    Authors: O Tufanli, P Telkoparan, D Acosta-Alv, B Kocaturk, UI Onat, SM Hamid, I €imen, P Walter, C Weber, E Erbay
    Proc. Natl. Acad. Sci. U.S.A, 2017;0(0):.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  10. SR-B1 Is a Silica Receptor that Mediates Canonical Inflammasome Activation
    Authors: M Tsugita, N Morimoto, M Tashiro, K Kinoshita, M Nakayama
    Cell Rep, 2017;18(5):1298-1311.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  11. Quercetin Inhibits Inflammasome Activation by Interfering with ASC Oligomerization and Prevents Interleukin-1 Mediated Mouse Vasculitis
    Authors: TP Domiciano, D Wakita, HD Jones, TR Crother, WA Verri, M Arditi, K Shimada
    Sci Rep, 2017;7(0):41539.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  12. Distinct Kinase-Independent Role of RIPK3 in CD11c(+) Mononuclear Phagocytes in Cytokine-Induced Tissue Repair
    Authors: K Moriwaki, S Balaji, J Bertin, PJ Gough, FK Chan
    Cell Rep, 2017;18(10):2441-2451.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  13. The P2X7 Receptor Primes IL-1? and the NLRP3 Inflammasome in Astrocytes Exposed to Mechanical Strain
    Authors: F Albalawi, W Lu, JM Beckel, JC Lim, SA McCaughey, CH Mitchell
    Front Cell Neurosci, 2017;11(0):227.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  14. MTG16 is a tumor suppressor in colitis-associated carcinoma
    Authors: EM McDonough, CW Barrett, B Parang, MK Mittal, JJ Smith, AM Bradley, YA Choksi, LA Coburn, SP Short, JJ Thompson, B Zhang, SV Poindexter, MA Fischer, X Chen, J Li, FL Revetta, R Naik, MK Washington, MJ Rosen, SW Hiebert, KT Wilson, CS Williams
    JCI Insight, 2017;2(16):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  15. Papilloma-pseudovirus eradicates intestinal tumours and triples the lifespan of Apc(Min/+) mice
    Authors: Z Zhong, Y Zhai, P Bu, S Shah, L Qiao
    Nat Commun, 2017;8(0):15004.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  16. IL-1? transcriptionally activates hepcidin by inducing C/EBP? expression in hepatocytes
    Authors: Y Kanamori, M Murakami, M Sugiyama, O Hashimoto, T Matsui, M Funaba
    J. Biol. Chem., 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  17. Lentinan from shiitake selectively attenuates AIM2 and non-canonical inflammasome activation while inducing pro-inflammatory cytokine production
    Authors: H Ahn, E Jeon, JC Kim, SG Kang, SI Yoon, HJ Ko, PH Kim, GS Lee
    Sci Rep, 2017;7(1):1314.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  18. AKT1 distinctively suppresses MyD88-depenedent and TRIF-dependent Toll-like receptor signaling in a kinase activity-independent manner
    Authors: K Zenke, M Muroi, KI Tanamoto
    Cell. Signal., 2017;43(0):32-39.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  19. The intracellular chloride channel proteins CLIC1 and CLIC4 induce IL-1? transcription and activate the NLRP3 inflammasome
    Authors: R Domingo-Fe, RC Coll, J Kearney, S Breit, LA O'Neill
    J. Biol. Chem., 2017;0(0):.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  20. NLR members NLRC4 and NLRP3 mediate sterile inflammasome activation in microglia and astrocytes
    Authors: L Freeman, H Guo, CN David, WJ Brickey, S Jha, JP Ting
    J. Exp. Med., 2017;0(0):.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  21. Interleukin-1 and estrogen protect against disseminating dentoalveolar infections
    Authors: H Youssef, P Stashenko
    Int J Oral Sci, 2017;9(1):16-23.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  22. TLR sensing of bacterial spore-associated RNA triggers host immune responses with detrimental effects
    Authors: MK Choo, Y Sano, C Kim, K Yasuda, XD Li, X Lin, M Stenzel-Po, L Alexopoulo, S Ghosh, E Latz, IR Rifkin, ZJ Chen, GC Stewart, H Chong, JM Park
    J. Exp. Med., 2017;214(5):1297-1311.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  23. Tetrahydrobiopterin (BH4) deficiency is associated with augmented inflammation and microvascular degeneration in the retina
    Authors: JC Rivera, B Noueihed, A Madaan, I Lahaie, J Pan, J Belik, S Chemtob
    J Neuroinflammation, 2017;14(1):181.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  24. The PYHIN Protein p205 Regulates the Inflammasome by Controlling Asc Expression
    Authors: S Ghosh, C Wallerath, S Covarrubia, V Hornung, S Carpenter, KA Fitzgerald
    J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  25. A new approach for ratiometric in vivo calcium imaging of microglia
    Authors: B Brawek, Y Liang, D Savitska, K Li, N Fomin-Thun, Y Kovalchuk, E Zirdum, J Jakobsson, O Garaschuk
    Sci Rep, 2017;7(1):6030.
  26. Nucleoside reverse transcriptase inhibitors (NRTIs) induce proinflammatory cytokines in the CNS via Wnt5a signaling
    Authors: T Wu, J Zhang, M Geng, SJ Tang, W Zhang, J Shu
    Sci Rep, 2017;7(1):4117.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  27. CD8+ T cells produce a dialyzable antigen-specific activator of dendritic cells
    J Leukoc Biol, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  28. Activation of NLRP3 inflammasomes in mouse hepatic stellate cells during Schistosoma J. infection
    Oncotarget, 2016;7(26):39316-39331.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IF
  29. Functional and Evolutionary Analyses Identify Proteolysis as a General Mechanism for NLRP1 Inflammasome Activation
    PLoS Pathog, 2016;12(12):e1006052.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  30. Histone deacetylase inhibitors mediate DNA damage repair in ameliorating hemorrhagic cystitis
    Sci Rep, 2016;6(0):39257.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  31. CARD9 negatively regulates NLRP3-induced IL-1? production on Salmonella infection of macrophages
    Nat Commun, 2016;7(0):12874.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  32. C13orf31 (FAMIN) is a central regulator of immunometabolic function
    Nat Immunol, 2016;0(0):.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  33. The Tick Protein Sialostatin L2 Binds to Annexin A2 and Inhibits NLRC4-Mediated Inflammasome Activation
    Authors: X Wang, DK Shaw, OS Sakhon, GA Snyder, EJ Sundberg, L Santambrog, FS Sutterwala, JS Dumler, KA Shirey, DJ Perkins, K Richard, AC Chagas, E Calvo, J Kopecký, M Kotsyfakis, JH Pedra
    Infect Immun, 2016;0(0):.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  34. HIF-1? Is an Essential Mediator of IFN-?-Dependent Immunity to Mycobacterium tuberculosis
    J Immunol, 2016;0(0):.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  35. Enzymatically inactive procaspase-1 stabilizes the ASC-pyroptosome and supports pyroptosome spreading during cell division
    J Biol Chem, 2016;0(0):.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: Bead-based Immunoassay
  36. Pyrin inflammasome activation and RhoA signaling in the autoinflammatory diseases FMF and HIDS
    Authors: Yong Hwan Park
    Nat Immunol, 2016;0(0):.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  37. Lymphatic vessels regulate immune microenvironments in human and murine melanoma
    J Clin Invest, 2016;0(0):.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: WB
  38. TNF?-stimulated gene-6 (TSG6) activates macrophage phenotype transition to prevent inflammatory lung injury
    Proc. Natl. Acad. Sci. U.S.A., 2016;113(50):E8151-E8158.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  39. Identification of QS-21 as an Inflammasome-activating Molecular Component of Saponin Adjuvants.
    Authors: Marty-Roix R, Vladimer G, Pouliot K, Weng D, Buglione-Corbett R, West K, Macmicking J, Chee J, Wang S, Lu S, Lien E
    J Biol Chem, 2016;291(3):1123-36.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  40. Local immunosuppressive microenvironment enhances migration of melanoma cells to lungs in DJ-1 knockout mice.
    Authors: Chien C, Lee M, Liou H, Liou H, Fu W
    PLoS ONE, 2015;10(2):e0115827.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  41. RIPK3 promotes cell death and NLRP3 inflammasome activation in the absence of MLKL.
    Authors: Lawlor K, Khan N, Mildenhall A, Gerlic M, Croker B, D'Cruz A, Hall C, Kaur Spall S, Anderton H, Masters S, Rashidi M, Wicks I, Alexander W, Mitsuuchi Y, Benetatos C, Condon S, Wong W, Silke J, Vaux D, Vince J
    Nat Commun, 2015;6(0):6282.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  42. Guanylate-binding proteins promote activation of the AIM2 inflammasome during infection with Francisella novicida.
    Authors: Meunier E, Wallet P, Dreier R, Costanzo S, Anton L, Ruhl S, Dussurgey S, Dick M, Kistner A, Rigard M, Degrandi D, Pfeffer K, Yamamoto M, Henry T, Broz P
    , 2015;0(0):.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  43. Retinal neurons curb inflammation and enhance revascularization in ischemic retinopathies via proteinase-activated receptor-2.
    Authors: Sitaras N, Rivera J, Noueihed B, Bien-Aime M, Zaniolo K, Omri S, Hamel D, Zhu T, Hardy P, Sapieha P, Joyal J, Chemtob S
    Am J Pathol, 2015;185(2):581-95.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  44. IL-10 engages macrophages to shift Th17 cytokine dependency and pathogenicity during T-cell-mediated colitis.
    Authors: Li B, Gurung P, Malireddi R, Vogel P, Kanneganti T, Geiger T
    Nat Commun, 2015;6(0):6131.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  45. AIM2 and NLRC4 inflammasomes contribute with ASC to acute brain injury independently of NLRP3.
    Authors: Denes A, Coutts G, Lenart N, Cruickshank S, Pelegrin P, Skinner J, Rothwell N, Allan S, Brough D
    Proc Natl Acad Sci U S A, 2015;112(13):4050-5.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  46. Small heterodimer partner interacts with NLRP3 and negatively regulates activation of the NLRP3 inflammasome.
    Authors: Yang C, Kim J, Kim T, Lee P, Kim S, Lee H, Shin D, Nguyen L, Lee M, Jin H, Kim K, Lee C, Kim M, Park S, Kim J, Choi H, Jo E
    Nat Commun, 2015;6(0):6115.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  47. Methylsulfonylmethane inhibits NLRP3 inflammasome activation.
    Authors: Ahn H, Kim J, Lee M, Kim Y, Cho Y, Lee G
    Cytokine, 2015;71(2):223-31.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: WB
  48. The cyclopentenone prostaglandin 15d-PGJ2 inhibits the NLRP1 and NLRP3 inflammasomes.
    Authors: Maier N, Leppla S, Moayeri M
    J Immunol, 2015;194(6):2776-85.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  49. Caspase-1-independent IL-1 release mediates blister formation in autoantibody-induced tissue injury through modulation of endothelial adhesion molecules.
    Authors: Sadeghi H, Lockmann A, Hund A, Samavedam U, Pipi E, Vafia K, Hauenschild E, Kalies K, Pas H, Jonkman M, Iwata H, Recke A, Schon M, Zillikens D, Schmidt E, Ludwig R
    J Immunol, 2015;194(8):3656-63.
  50. Deficient NLRP3 and AIM2 Inflammasome Function in Autoimmune NZB Mice.
    Authors: Sester D, Sagulenko V, Thygesen S, Cridland J, Loi Y, Cridland S, Masters S, Genske U, Hornung V, Andoniou C, Sweet M, Degli-Esposti M, Schroder K, Stacey K
    J Immunol, 2015;195(3):1233-41.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: WB
  51. 3,4-methylenedioxy-beta-nitrostyrene inhibits NLRP3 inflammasome activation by blocking assembly of the inflammasome.
    Authors: He Y, Varadarajan S, Munoz-Planillo R, Burberry A, Nakamura Y, Nunez G
    J Biol Chem, 2014;289(2):1142-50.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  52. Critical role for IL-1beta in DNA damage-induced mucositis.
    Authors: Kanarek N, Grivennikov S, Leshets M, Lasry A, Alkalay I, Horwitz E, Shaul Y, Stachler M, Voronov E, Apte R, Pagano M, Pikarsky E, Karin M, Ghosh S, Ben-Neriah Y
    Proc Natl Acad Sci U S A, 2014;111(6):E702-11.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  53. The frustrated host response to Legionella pneumophila is bypassed by MyD88-dependent translation of pro-inflammatory cytokines.
    Authors: Asrat S, Dugan A, Isberg R
    PLoS Pathog, 2014;10(7):e1004229.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  54. Activation of NLRP3 inflammasome by crystalline structures via cell surface contact.
    Authors: Hari A, Zhang Y, Tu Z, Detampel P, Stenner M, Ganguly A, Shi Y
    Sci Rep, 2014;4(0):7281.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  55. Signaling pathways in murine dendritic cells that regulate the response to vesicular stomatitis virus vectors that express flagellin.
    Authors: Smedberg J, Westcott M, Ahmed M, Lyles D
    J Virol, 2014;88(2):777-85.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  56. Severe acute respiratory syndrome coronavirus envelope protein ion channel activity promotes virus fitness and pathogenesis.
    Authors: Nieto-Torres J, DeDiego M, Verdia-Baguena C, Jimenez-Guardeno J, Regla-Nava J, Fernandez-Delgado R, Castano-Rodriguez C, Alcaraz A, Torres J, Aguilella V, Enjuanes L
    PLoS Pathog, 2014;10(5):e1004077.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  57. Caspase-11 activation requires lysis of pathogen-containing vacuoles by IFN-induced GTPases.
    Authors: Meunier E, Dick M, Dreier R, Schurmann N, Kenzelmann Broz D, Warming S, Roose-Girma M, Bumann D, Kayagaki N, Takeda K, Yamamoto M, Broz P
    Nature, 2014;509(7500):366-70.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: WB
  58. Caspase-8 modulates dectin-1 and complement receptor 3-driven IL-1beta production in response to beta-glucans and the fungal pathogen, Candida albicans.
    Authors: Ganesan S, Rathinam V, Bossaller L, Army K, Kaiser W, Mocarski E, Dillon C, Green D, Mayadas T, Levitz S, Hise A, Silverman N, Fitzgerald K
    J Immunol, 2014;193(5):2519-30.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  59. Nuclear factor E2-related factor-2 (Nrf2) is required for NLRP3 and AIM2 inflammasome activation.
    Authors: Zhao C, Gillette D, Li X, Zhang Z, Wen H
    J Biol Chem, 2014;289(24):17020-9.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  60. A critical role for human caspase-4 in endotoxin sensitivity.
    Authors: Kajiwara Y, Schiff T, Voloudakis G, Gama Sosa M, Elder G, Bozdagi O, Buxbaum J
    J Immunol, 2014;193(1):335-43.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  61. Systemic autophagy insufficiency compromises adaptation to metabolic stress and facilitates progression from obesity to diabetes.
    Authors: Lim, Yu-Mi, Lim, Hyejin, Hur, Kyu Yeon, Quan, Wenying, Lee, Hae-Youn, Cheon, Hwanju, Ryu, Dongryeo, Koo, Seung-Ho, Kim, Hong Lim, Kim, Jin, Komatsu, Masaaki, Lee, Myung-Sh
    Nat Commun, 2014;5(0):4934.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  62. Critical role of aquaporins in interleukin 1beta (IL-1beta)-induced inflammation.
    Authors: Rabolli V, Wallemme L, Lo Re S, Uwambayinema F, Palmai-Pallag M, Thomassen L, Tyteca D, Octave J, Marbaix E, Lison D, Devuyst O, Huaux F
    J Biol Chem, 2014;289(20):13937-47.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  63. Endothelial NLRP3 inflammasome activation and enhanced neointima formation in mice by adipokine visfatin.
    Authors: Xia M, Boini K, Abais J, Xu M, Zhang Y, Li P
    Am J Pathol, 2014;184(5):1617-28.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  64. Arsenic trioxide and other arsenical compounds inhibit the NLRP1, NLRP3, and NAIP5/NLRC4 inflammasomes.
    Authors: Maier N, Crown D, Liu J, Leppla S, Moayeri M
    J Immunol, 2014;192(2):763-70.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  65. RNA and beta-hemolysin of group B Streptococcus induce interleukin-1beta (IL-1beta) by activating NLRP3 inflammasomes in mouse macrophages.
    Authors: Gupta R, Ghosh S, Monks B, DeOliveira R, Tzeng T, Kalantari P, Nandy A, Bhattacharjee B, Chan J, Ferreira F, Rathinam V, Sharma S, Lien E, Silverman N, Fitzgerald K, Firon A, Trieu-Cuot P, Henneke P, Golenbock D
    J Biol Chem, 2014;289(20):13701-5.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: WB
  66. Activation of microglia induces symptoms of Parkinson's disease in wild-type, but not in IL-1 knockout mice.
    Authors: Tanaka S, Ishii A, Ohtaki H, Shioda S, Yoshida T, Numazawa S
    J Neuroinflammation, 2013;10(0):143.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  67. Wingless-type mammary tumor virus integration site family, member 5A (Wnt5a) regulates human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein 120 (gp120)-induced expression of pro-inflammatory cytokines via the Ca2+/calmodulin-dependent protein kinase II (CaMKII) and c-Jun N-terminal kinase (JNK) signaling pathways.
    Authors: Li B, Shi Y, Shu J, Gao J, Wu P, Tang S
    J Biol Chem, 2013;288(19):13610-9.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  68. Microtubule-driven spatial arrangement of mitochondria promotes activation of the NLRP3 inflammasome.
    Authors: Misawa T, Takahama M, Kozaki T, Lee H, Zou J, Saitoh T, Akira S
    Nat Immunol, 2013;14(5):454-60.
    Species: Mouse
    Sample Type: Peritoneal Fluid
    Application: WB
  69. Gli1 deletion prevents Helicobacter-induced gastric metaplasia and expansion of myeloid cell subsets.
    Authors: El-Zaatari M, Kao J, Tessier A, Bai L, Hayes M, Fontaine C, Eaton K, Merchant J
    PLoS ONE, 2013;8(3):e58935.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Fluorescence
  70. The role of IL-1beta in the early tumor cell-induced angiogenic response.
    Authors: Carmi Y, Dotan S, Rider P, Kaplanov I, White M, Baron R, Abutbul S, Huszar M, Dinarello C, Apte R, Voronov E
    J Immunol, 2013;190(7):3500-9.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  71. The calcium-sensing receptor regulates the NLRP3 inflammasome through Ca2+ and cAMP.
    Authors: Lee G, Subramanian N, Kim A, Aksentijevich I, Goldbach-Mansky R, Sacks D, Germain R, Kastner D, Chae J
    Nature, 2012;492(7427):123-7.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: WB
  72. Caspase-11 increases susceptibility to Salmonella infection in the absence of caspase-1.
    Authors: Broz P, Ruby T, Belhocine K, Bouley D, Kayagaki N, Dixit V, Monack D
    Nature, 2012;490(7419):288-91.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: WB
  73. Dectin-1-dependent interleukin-22 contributes to early innate lung defense against Aspergillus fumigatus.
    Authors: Gessner MA, Werner JL, Lilly LM, Nelson MP, Metz AE, Dunaway CW, Chan YR, Ouyang W, Brown GD, Weaver CT, Steele C
    Infect. Immun., 2012;80(1):410-7.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  74. IL-6 trans-signaling licenses mouse and human tumor microvascular gateways for trafficking of cytotoxic T cells.
    Authors: Fisher DT, Chen Q, Skitzki JJ, Muhitch JB, Zhou L, Appenheimer MM, Vardam TD, Weis EL, Passanese J, Wang WC, Gollnick SO, Dewhirst MW, Rose-John S, Repasky EA, Baumann H, Evans SS
    J. Clin. Invest., 2011;121(10):3846-59.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  75. Coordinated Host Responses during Pyroptosis: Caspase-1-Dependent Lysosome Exocytosis and Inflammatory Cytokine Maturation.
    Authors: Bergsbaken T, Fink SL, den Hartigh AB, Loomis WP, Cookson BT
    J. Immunol., 2011;187(5):2748-54.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: WB
  76. Microenvironment-derived IL-1 and IL-17 interact in the control of lung metastasis.
    Authors: Carmi Y, Rinott G, Dotan S, Elkabets M, Rider P, Voronov E, Apte RN
    J. Immunol., 2011;186(6):3462-71.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  77. IL-1alpha and IL-1beta recruit different myeloid cells and promote different stages of sterile inflammation.
    Authors: Rider P, Carmi Y, Guttman O, Braiman A, Cohen I, Voronov E, White MR, Dinarello CA, Apte RN
    J. Immunol., 2011;187(9):4835-43.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  78. Toll-like receptor 2 contributes to chemokine gene expression and macrophage infiltration in the dorsal root ganglia after peripheral nerve injury.
    Authors: Kim D, You B, Lim H, Lee SJ
    Mol Pain, 2011;7(0):74.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  79. Cytotoxins of the human pathogen Aeromonas hydrophila trigger, via the NLRP3 inflammasome, caspase-1 activation in macrophages.
    Authors: McCoy AJ, Koizumi Y, Toma C, Higa N, Dixit V, Taniguchi S, Tschopp J, Suzuki T
    Eur. J. Immunol., 2010;40(10):2797-803.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  80. Interleukin-1beta mediates the extra-intestinal thrombosis associated with experimental colitis.
    Authors: Yoshida H, Russell J, Senchenkova EY, Almeida Paula LD, Granger DN
    Am. J. Pathol., 2010;177(6):2774-81.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  81. Tyrosine kinase 2 controls IL-1beta production at the translational level.
    Authors: Radwan M, Stiefvater R, Grunert T, Sharif O, Miller I, Marchetti-Deschmann M, Allmaier G, Gemeiner M, Knapp S, Kovarik P, Muller M, Strobl B
    J. Immunol., 2010;185(6):3544-53.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  82. Activation of the NLRP3 inflammasome by islet amyloid polypeptide provides a mechanism for enhanced IL-1beta in type 2 diabetes.
    Authors: Masters SL, Dunne A, Subramanian SL, Hull RL, Tannahill GM, Sharp FA, Becker C, Franchi L, Yoshihara E, Chen Z, Mullooly N, Mielke LA, Harris J, Coll RC, Mills KH, Mok KH, Newsholme P, Nunez G, Yodoi J, Kahn SE, Lavelle EC, O'Neill LA
    Nat. Immunol., 2010;11(10):897-904.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  83. Chlamydial and periodontal pathogens induce hepatic inflammation and fatty acid imbalance in apolipoprotein E-deficient mice.
    Authors: Hyvarinen K, Tuomainen AM, Laitinen S, Bykov IL, Tormakangas L, Lindros K, Käkelä R, Alfthan G, Salminen I, Jauhiainen M, Kovanen PT, Leinonen M, Saikku P, Pussinen PJ
    Infect. Immun., 2009;77(8):3442-9.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  84. Externalization of the leaderless cytokine IL-1F6 occurs in response to lipopolysaccharide/ATP activation of transduced bone marrow macrophages.
    Authors: Martin U, Scholler J, Gurgel J, Renshaw B, Sims JE, Gabel CA
    J. Immunol., 2009;183(6):4021-30.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  85. Activation of the NLRP3 inflammasome in dendritic cells induces IL-1beta-dependent adaptive immunity against tumors.
    Authors: Ghiringhelli F, Apetoh L, Tesniere A, Aymeric L, Ma Y, Ortiz C, Vermaelen K, Panaretakis T, Mignot G, Ullrich E, Perfettini JL, Schlemmer F, Tasdemir E, Uhl M, Genin P, Civas A, Ryffel B, Kanellopoulos J, Tschopp J, Andre F, Lidereau R, McLaughlin NM, Haynes NM, Smyth MJ, Kroemer G, Zitvogel L
    Nat. Med., 2009;15(10):1170-8.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  86. Japanese Encephalitis Virus infection induces IL-18 and IL-1beta in microglia and astrocytes: correlation with in vitro cytokine responsiveness of glial cells and subsequent neuronal death.
    Authors: Das S, Mishra MK, Ghosh J, Basu A
    J. Neuroimmunol., 2008;195(1):60-72.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  87. Stimulation of Toll-like receptor 3 and 4 induces interleukin-1beta maturation by caspase-8.
    Authors: Maelfait J, Vercammen E, Janssens S, Schotte P, Haegman M, Magez S, Beyaert R
    J. Exp. Med., 2008;205(9):1967-73.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  88. Toll-like receptor 4 (TLR4)-dependent proinflammatory and immunomodulatory properties of the glycoinositolphospholipid (GIPL) from Trypanosoma cruzi.
    Authors: Medeiros MM, Peixoto JR, Oliveira AC, Cardilo-Reis L, Koatz VL, Van Kaer L, Previato JO, Mendonca-Previato L, Nobrega A, Bellio M
    J. Leukoc. Biol., 2007;82(3):488-96.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  89. Topical acetone treatment induces neurogenic oedema on the sensitized mouse ear: an in vivo study using transient receptor potential vanilloid 1 (TRPV1) receptor knockout mice.
    Authors: Pozsgai G, Sandor K, Perkecz A, Szolcsanyi J, Helyes Z, Brain SD, Pinter E
    Inflamm. Res., 2007;56(11):459-67.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  90. Granulocyte colony-stimulating factor impairs liver regeneration in mice through the up-regulation of interleukin-1beta.
    Authors: Ogiso T, Nagaki M, Takai S, Tsukada Y, Mukai T, Kimura K, Moriwaki H
    J. Hepatol., 2007;47(6):816-25.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  91. Inflammasome-mediated production of IL-1beta is required for neutrophil recruitment against Staphylococcus aureus in vivo.
    Authors: Miller LS, Pietras EM, Uricchio LH, Hirano K, Rao S, Lin H, O'Connell RM, Iwakura Y, Cheung AL, Cheng G, Modlin RL
    J. Immunol., 2007;179(10):6933-42.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  92. The Myc-dependent angiogenic switch in tumors is mediated by interleukin 1beta.
    Authors: Shchors K, Shchors E, Rostker F, Lawlor ER, Brown-Swigart L, Evan GI
    Genes Dev., 2006;20(18):2527-38.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  93. Involvement of monocyte chemoattractant protein-1, macrophage inflammatory protein-1alpha and interleukin-1beta in Wallerian degeneration.
    Authors: Perrin FE, Lacroix S, Aviles-Trigueros M, David S
    Brain, 2005;128(0):854-66.
    Species: Mouse
    Sample Type:
    Application: In vivo
  94. Intravenous human interleukin-1alpha impairs memory processing in mice: dependence on blood-brain barrier transport into posterior division of the septum.
    Authors: Banks WA, Farr SA, La Scola ME
    J. Pharmacol. Exp. Ther., 2001;299(2):536-41.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  95. Biglycan, a danger signal that activates the NLRP3 inflammasome via toll-like and P2X receptors.
    Authors: Babelova A, Moreth K, Tsalastra-Greul W, Zeng-Brouwers J, Eickelberg O, Young MF, Bruckner P, Pfeilschifter J, Schaefer RM, Schaefer R, Grone HJ, Schaefer L
    J Biol Chem, 0;284(36):24035-48.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
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Average Rating: 4 (Based on 7 reviews)

Have you used Mouse IL-1 beta /IL-1F2 Antibody?

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Read what people are saying who have used Mouse IL-1 beta /IL-1F2 Antibody.

We have 7 reviews tested in 2 species: Human, Mouse.
We have 7 reviews tested in 4 applications: ELISA, Western Blot, Flow Cytometry, Block/Neutralize.

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Images Ratings Applications Species Reviewed By Date Details
  Average
 ELISA Human Anonymous 05/17/2018
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Summary

Application ELISA
Sample Tested A549 human lung carcinoma cell line
Species Human
  Very Good
 WB Mouse Anonymous 03/22/2018
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Summary

Application Western Blot
Sample Tested IC-21 mouse macrophage cell line
Species Mouse
Western Blot Mouse IL-1 beta /IL-1F2 Antibody AF-401-NA Array AF-401-NA
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Very Good
 WB Mouse Anonymous 10/31/2017
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Western Blot Mouse IL-1 beta /IL-1F2 Antibody AF-401-NA
Western Blot: Mouse IL-1 beta /IL-1F2 Antibody [AF-401-NA].

Summary

Application Western Blot
Sample Tested bone marrow derived macrophage
Species Mouse
  Very Good
 Flow Mouse Anonymous 09/08/2017
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Summary

Application Flow Cytometry
Sample Tested L-929 mouse fibroblast cell line
Species Mouse
  Excellent
 Block/Neutralize Mouse Anonymous 04/26/2017
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Summary

Application Block/Neutralize
Sample Tested Adult splenocytes
Species Mouse
Western Blot Mouse IL-1 beta /IL-1F2 Antibody AF-401-NA Array AF-401-NA
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Average
 WB Mouse Anonymous 04/25/2017
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Western Blot Mouse IL-1 beta /IL-1F2 Antibody AF-401-NA
Western Blot: Mouse IL-1 beta /IL-1F2 Antibody [AF-401-NA].

Summary

Application Western Blot
Sample Tested Cell culture supernatant,Serum-free Cell Culture Supernates,Bone marrow cells
Species Mouse
Western Blot Mouse IL-1 beta /IL-1F2 Antibody AF-401-NA Array AF-401-NA
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Excellent
 WB Mouse Anonymous 02/14/2017
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Western Blot Mouse IL-1 beta /IL-1F2 Antibody AF-401-NA
Western Blot: Mouse IL-1 beta /IL-1F2 Antibody [AF-401-NA].

Summary

Application Western Blot
Sample Tested bone marrow derived macrophages
Species Mouse

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