Home / IL-1 beta/IL-1F2 / Mouse IL-1 beta /IL-1F2 Antibody

Mouse IL-1 beta /IL-1F2 Antibody

AF-401-NA 151 Citations 16 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF-401-NA
AF-401-SP
Mouse IL-1 beta /IL-1F2 Antibody in Data
5 Images
Product Details
Citations (151)
FAQs
Supplemental Products
Reviews (16)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IL-1 beta /IL-1F2 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-1 beta /IL-1F2 in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant porcine IL‑1 beta is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse IL-1 beta /IL-1F2
Val118-Ser269
Accession # NP_032387
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.25 µg/mL
See below
Simple Western
2.5 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize IL‑1 beta /IL‑1F2-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND50) is typically ≤0.25 µg/mL in the presence of 50 pg/mL Recombinant Mouse IL‑1 beta /IL‑1F2.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human and Mouse IL-1 beta /IL-1F2 antibody by Western Blot. View Larger

Detection of Human and Mouse IL‑1 beta /IL‑1F2 by Western Blot. Western blot shows lysates of THP-1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nM PMA for 24 hours and 10 µg/mL LPS for 4 hours and RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with 10 µg/mL LPS for 24 hours. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse IL-1 beta /IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-401-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL-1 beta /IL-1F2 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry IL-1 beta /IL-1F2 antibody in MCF-7 Human Cell Line by Immunocytochemistry (ICC). View Larger

IL‑1 beta /IL‑1F2 in MCF‑7 Human Cell Line. IL-1 beta /IL-1F2 was detected in immersion fixed MCF-7 human breast cancer cell line using Goat Anti-Mouse IL-1 beta /IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-401-NA) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry IL-1 beta /IL-1F2 antibody in Mouse Thymus by Immunohistochemistry (IHC-Fr). View Larger

IL‑1 beta /IL‑1F2 in Mouse Thymus. IL-1 beta /IL-1F2 was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse IL-1 beta /IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-401-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Neutralization Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutralization by Mouse IL-1 beta /IL-1F2 Antibody. View Larger

Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutralization by Mouse IL-1 beta /IL-1F2 Antibody. Recombinant Mouse IL-1 beta /IL-1F2 (Catalog # 401-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Mouse IL-1 beta /IL-1F2 (50 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-1 beta /IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-401-NA). The ND50is typically =0.25 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-1 beta/IL-1F2

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2, IL1B), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 17% amino acid (aa) identity in mouse. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. The 17 kDa molecular weight mature mouse IL-1 beta shares 90% aa sequence identity with cotton rat and rat and 67%-78% with canine, equine, feline, human, porcine, and rhesus macaque IL-1 beta. IL-1 beta functions in a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases.

Long Name
Interleukin 1 beta
Entrez Gene IDs
3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
Alternate Names
catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta

Product Datasheets

Product Datasheet
COA
SDS

Citations for Mouse IL-1 beta /IL-1F2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

151 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Slc6a3-dependent expression of a CAPS-associated Nlrp3 allele results in progressive behavioral abnormalities and neuroinflammation in aging mice
    Authors: KM von Herrma, FL Anderson, EM Martinez, AL Young, MC Havrda
    J Neuroinflammation, 2020;17(1):213.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. A Two-Cell Model for IL-1&beta Release Mediated by Death-Receptor Signaling
    Authors: CA Donado, AB Cao, DP Simmons, BA Croker, PJ Brennan, MB Brenner
    Cell Rep, 2020;31(1):107466.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Western Blot
  3. Involvement of exchange protein directly activated by cAMP and tumor progression locus 2 in IL-1&beta production in microglial cells following activation of &beta-adrenergic receptors
    Authors: H Tozaki-Sai, I Sasaki, T Yamashita, M Hosoi, TA Kato, M Tsuda
    J. Pharmacol. Sci., 2020;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Myeloid PTEN promotes chemotherapy-induced NLRP3-inflammasome activation and antitumour immunity
    Authors: Y Huang, H Wang, Y Hao, H Lin, M Dong, J Ye, L Song, Y Wang, Q Li, B Shan, Y Jiang, H Li, Z Shao, G Kroemer, H Zhang, L Bai, T Jin, C Wang, Y Ma, Y Cai, C Ding, S Liu, Y Pan, W Jiang, R Zhou
    Nat. Cell Biol., 2020;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. ELMO2 association with G&alphai2 regulates pancreatic cancer cell chemotaxis and metastasis
    Authors: Y Wang, H Li, F Li
    PeerJ, 2020;8(0):e8910.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. I&kappaB? controls NLRP3 inflammasome activation via upregulation of the Nlrp3 gene
    Authors: J Kim, H Ahn, S Yu, JH Ahn, HJ Ko, MN Kweon, EJ Hong, BS An, E Lee, GS Lee
    Cytokine, 2020;127(0):154983.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Suppression of NLRP3 Inflammasome by Erythropoietin via the EPOR/JAK2/STAT3 Pathway Contributes to Attenuation of Acute Lung Injury in Mice
    Authors: F Cao, X Tian, Z Li, Y Lv, J Han, R Zhuang, B Cheng, Y Gong, B Ying, S Jin, Y Gao
    Front Pharmacol, 2020;11(0):306.
    Species: Mouse
    Sample Types: Tissue Lysates
    Applications: Western Blot
  8. Identification of hub genes and key pathways of dietary advanced glycation end products?induced non?alcoholic fatty liver disease by bioinformatics analysis and animal experiments
    Authors: J Wang, H Liu, G Xie, W Cai, J Xu
    Mol Med Rep, 2020;21(2):685-694.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. Inhibition of double-strand DNA-sensing cGAS ameliorates brain injury after ischemic stroke
    Authors: Q Li, Y Cao, C Dang, B Han, R Han, H Ma, J Hao, L Wang
    EMBO Mol Med, 2020;12(4):e11002.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. Oncogenic KrasG12D causes myeloproliferation via NLRP3 inflammasome activation
    Authors: S Hamarsheh, L Osswald, BS Saller, S Unger, D De Feo, JM Vinnakota, M Konantz, FM Uhl, H Becker, M Lübbert, K Shoumariye, C Schürch, G Andrieux, N Venhoff, A Schmitt-Gr, S Duquesne, D Pfeifer, MA Cooper, C Lengerke, M Boerries, J Duyster, CM Niemeyer, M Erlacher, BR Blazar, B Becher, O Gro beta, T Brummer, R Zeiser
    Nat Commun, 2020;11(1):1659.
    Species: Mouse
    Sample Types: Cell Culture Lysates
    Applications: Western Blot
  11. Ptpn6 inhibits caspase-8- and Ripk3/Mlkl-dependent inflammation
    Authors: M Speir, CJ Nowell, AA Chen, JA O'Donnell, IS Shamie, PR Lakin, AA D'Cruz, RO Braun, JJ Babon, RS Lewis, M Bliss-More, I Shlomovitz, S Wang, LH Cengia, AI Stoica, R Hakem, MA Kelliher, LA O'Reilly, H Patsiouras, KE Lawlor, E Weller, NE Lewis, AW Roberts, M Gerlic, BA Croker
    Nat. Immunol., 2019;21(1):54-64.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  12. Protein prenylation restrains innate immunity by inhibiting Rac1 effector interactions
    Authors: MK Akula, MX Ibrahim, EG Ivarsson, OM Khan, IT Kumar, M Erlandsson, C Karlsson, X Xu, M Brisslert, C Brakebusch, D Wang, M Bokarewa, VI Sayin, MO Bergo
    Nat Commun, 2019;10(1):3975.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  13. IL-25 exacerbates autoimmune aortitis in IL-1 receptor antagonist-deficient mice
    Authors: T Yoshizaki, S Itoh, S Yamaguchi, T Numata, A Nambu, N Kimura, H Suto, K Okumura, K Sudo, A Yamaguchi, S Nakae
    Sci Rep, 2019;9(1):17067.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  14. CCL28 promotes locomotor recovery after spinal cord injury via recruiting regulatory T cells
    Authors: P Wang, X Qi, G Xu, J Liu, J Guo, X Li, X Ma, H Sun
    Aging (Albany NY), 2019;11(18):7402-7415.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  15. DDX3X acts as a live-or-die checkpoint in stressed cells by regulating NLRP3 inflammasome
    Authors: P Samir, S Kesavardha, DM Patmore, S Gingras, RKS Malireddi, R Karki, CS Guy, B Briard, DE Place, A Bhattachar, BR Sharma, A Nourse, SV King, A Pitre, AR Burton, S Pelletier, RJ Gilbertson, TD Kanneganti
    Nature, 2019;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  16. Gasdermin D Drives the Nonexosomal Secretion of Galectin-3, an Insulin Signal Antagonist
    Authors: Y Chen, H Wang, J Shen, R Deng, X Yao, Q Guo, A Lu, B Sun, Y Zhang, G Meng
    J. Immunol., 2019;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  17. Endogenous oxidized phospholipids reprogram cellular metabolism and boost hyperinflammation
    Authors: M Di Gioia, R Spreafico, JR Springstea, MM Mendelson, R Joehanes, D Levy, I Zanoni
    Nat. Immunol., 2019;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  18. Protein tyrosine phosphatase non-receptor type 22 modulates colitis in a microbiota-dependent manner
    Authors: MR Spalinger, TS Schmidt, M Schwarzfis, L Hering, K Atrott, S Lang, C Gottier, A Geirnaert, C Lacroix, X Dai, DJ Rawlings, AC Chan, C von Mering, G Rogler, M Scharl
    J. Clin. Invest., 2019;130(0):2527-2541.
    Species: Mouse
    Sample Types: Tissue Lysates
    Applications: Western Blot
  19. Dicer regulates activation of the NLRP3 inflammasome
    Authors: DM Ojcius, A Jafari, L Yeruva, CW Schindler, AA Abdul-Sate
    PLoS ONE, 2019;14(4):e0215689.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  20. Glutathione Transferase Omega-1 Regulates NLRP3 Inflammasome Activation through NEK7 Deglutathionylation
    Authors: MM Hughes, A Hooftman, S Angiari, P Tummala, Z Zaslona, MC Runtsch, AF McGettrick, CE Sutton, C Diskin, M Rooke, S Takahashi, S Sundararaj, MG Casarotto, JE Dahlstrom, EM Palsson-Mc, SC Corr, KHG Mills, RJS Preston, N Neamati, Y Xie, JB Baell, PG Board, LAJ O'Neill
    Cell Rep, 2019;29(1):151-161.e5.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  21. A Mitochondrial Micropeptide Is Required for Activation of the Nlrp3 Inflammasome
    Authors: A Bhatta, M Atianand, Z Jiang, J Crabtree, J Blin, KA Fitzgerald
    J. Immunol., 2019;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  22. The lncRNA Neat1 promotes activation of inflammasomes in macrophages
    Authors: P Zhang, L Cao, R Zhou, X Yang, M Wu
    Nat Commun, 2019;10(1):1495.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  23. RNA viruses promote activation of the NLRP3 inflammasome through cytopathogenic effect-induced potassium efflux
    Authors: LS da Costa, A Outlioua, A Anginot, K Akarid, D Arnoult
    Cell Death Dis, 2019;10(5):346.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  24. Nonsaponin fraction of Korean Red Ginseng attenuates cytokine production via inhibition of TLR4 expression
    Authors: H Ahn, BC Han, J Kim, SG Kang, PH Kim, KH Jang, SH So, SH Lee, GS Lee
    J Ginseng Res, 2019;43(2):291-299.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  25. High mobility group box 1 enables bacterial lipids to trigger receptor-interacting protein kinase 3 (RIPK3)-mediated necroptosis and apoptosis in mice
    Authors: R Meng, L Gu, Y Lu, K Zhao, J Wu, H Wang, J Han, Y Tang, B Lu
    J. Biol. Chem., 2019;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  26. Dynamic role of the codon 72 p53 single-nucleotide polymorphism in mammary tumorigenesis in a humanized mouse model
    Authors: RT Gunaratna, A Santos, L Luo, C Nagi, I Lambertz, M Spier, CJ Conti, RS Fuchs-Youn
    Oncogene, 2019;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  27. Tumor stem-like cell-derived exosomal RNAs prime neutrophils for facilitating tumorigenesis of colon cancer
    Authors: WL Hwang, HY Lan, WC Cheng, SC Huang, MH Yang
    J Hematol Oncol, 2019;12(1):10.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  28. Macrophages, rather than DCs, are responsible for inflammasome activity in the GM-CSF BMDC model
    Authors: Z Erlich, I Shlomovitz, L Edry-Botze, H Cohen, D Frank, H Wang, AM Lew, KE Lawlor, Y Zhan, JE Vince, M Gerlic
    Nat. Immunol., 2019;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  29. Injury induces endothelial to mesenchymal transition in the mouse corneal endothelium in vivo via FGF2
    Authors: J Lee, E Jung, M Heur
    Mol. Vis., 2019;25(0):22-34.
    Species: Mouse
    Sample Types: Aqueous Humor
    Applications: Western Blot
  30. Responses of perivascular macrophages to circulating lipopolysaccharides in the subfornical organ with special reference to endotoxin tolerance
    Authors: S Morita-Tak, K Nakahara, S Hasegawa-I, A Isonishi, K Tatsumi, H Okuda, T Tanaka, M Kitabatake, T Ito, A Wanaka
    J Neuroinflammation, 2019;16(1):39.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  31. SIRT3 diminishes inflammation and mitigates endotoxin-induced acute lung injury
    Authors: D Kurundkar, AR Kurundkar, NB Bone, EJ Becker, W Liu, B Chacko, V Darley-Usm, JW Zmijewski, VJ Thannickal
    JCI Insight, 2019;4(1):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  32. Long noncoding RNA Malat1 regulates differential activation of macrophage and response to lung injury
    Authors: H Cui, S Banerjee, S Guo, N Xie, J Ge, D Jiang, M Zörnig, VJ Thannickal, G Liu
    JCI Insight, 2019;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  33. NLRP3 inflammasome inhibitor OLT1177 suppresses joint inflammation in murine models of acute arthritis
    Authors: C Marchetti, B Swartzwelt, MI Koenders, T Azam, IW Tengesdal, N Powers, DM de Graaf, CA Dinarello, LAB Joosten
    Arthritis Res. Ther., 2018;20(1):169.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  34. TNF/TNFR axis promotes pyrin inflammasome activation and distinctly modulates pyrin inflammasomopathy
    Authors: D Sharma, A Malik, C Guy, P Vogel, TD Kanneganti
    J. Clin. Invest., 2018;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  35. Sex differences in primary muscle afferent sensitization following ischemia and reperfusion injury
    Authors: JL Ross, LF Queme, JE Lamb, KJ Green, MP Jankowski
    Biol Sex Differ, 2018;9(1):2.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  36. Caspase-1 self-cleavage is an intrinsic mechanism to terminate inflammasome activity
    Authors: D Boucher, M Monteleone, RC Coll, KW Chen, CM Ross, JL Teo, GA Gomez, CL Holley, D Bierschenk, KJ Stacey, AS Yap, JS Bezbradica, K Schroder
    J. Exp. Med., 2018;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  37. An unexpected link between fatty acid synthase and cholesterol synthesis in proinflammatory macrophage activation
    Authors: RG Carroll, Z Zas?ona, S Galván-Peñ, EL Koppe, DC Sévin, S Angiari, M Triantafil, K Triantafil, LK Modis, LA O'Neill
    J. Biol. Chem., 2018;293(15):5509-5521.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  38. Artemisia Extract Suppresses NLRP3 and AIM2 Inflammasome Activation by Inhibition of ASC Phosphorylation
    Authors: SB Kwak, S Koppula, EJ In, X Sun, YK Kim, MK Kim, KH Lee, TB Kang
    Mediators Inflamm., 2018;2018(0):6054069.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  39. A Metabolic Checkpoint for the Yeast-to-Hyphae Developmental Switch Regulated by Endogenous Nitric Oxide Signaling
    Authors: B Koch, AA Barugahare, TL Lo, C Huang, RB Schittenhe, DR Powell, TH Beilharz, A Traven
    Cell Rep, 2018;25(8):2244-2258.e7.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  40. Interleukin-1? Maturation Triggers Its Relocation to the Plasma Membrane for Gasdermin-D-Dependent and -Independent Secretion
    Authors: M Monteleone, AC Stanley, KW Chen, DL Brown, JS Bezbradica, JB von Pein, CL Holley, D Boucher, MR Shakespear, R Kapetanovi, V Rolfes, MJ Sweet, JL Stow, K Schroder
    Cell Rep, 2018;24(6):1425-1433.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  41. The Mitochondrial Apoptotic Effectors BAX/BAK Activate Caspase-3 and -7 to Trigger NLRP3 Inflammasome and Caspase-8 Driven IL-1? Activation
    Authors: JE Vince, D De Nardo, W Gao, AJ Vince, C Hall, K McArthur, D Simpson, S Vijayaraj, LM Lindqvist, P Bouillet, MA Rizzacasa, SM Man, J Silke, SL Masters, G Lessene, DCS Huang, DHD Gray, BT Kile, F Shao, KE Lawlor
    Cell Rep, 2018;25(9):2339-2353.e4.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  42. Single-Cell RNA-Seq Reveals the Transcriptional Landscape and Heterogeneity of Aortic Macrophages in Murine Atherosclerosis
    Authors: C Cochain, E Vafadarnej, P Arampatzi, P Jaroslav, H Winkels, K Ley, D Wolf, AE Saliba, A Zernecke
    Circ. Res., 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  43. The Traditional Chinese Medicine MLC901 inhibits inflammation processes after focal cerebral ischemia
    Authors: C Widmann, C Gandin, A Petit-Pait, M Lazdunski, C Heurteaux
    Sci Rep, 2018;8(1):18062.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  44. A multicomponent toxin from Bacillus cereus incites inflammation and shapes host outcome via the NLRP3 inflammasome
    Authors: A Mathur, S Feng, JA Hayward, C Ngo, D Fox, II Atmosukart, JD Price, K Schauer, E Märtlbauer, AAB Robertson, G Burgio, EM Fox, SH Leppla, NO Kaakoush, SM Man
    Nat Microbiol, 2018;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  45. Cutting Edge: IL-1? and Not IL-1? Drives IL-1R1-Dependent Neonatal Murine Sepsis Lethality
    Authors: JT Benjamin, DJ Moore, C Bennett, R van der Me, A Royce, R Loveland, JL Wynn
    J. Immunol., 2018;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  46. MCMV triggers ROS/NLRP3-associated inflammasome activation in the inner ear of mice and cultured spiral ganglion neurons, contributing to sensorineural hearing loss
    Authors: W Zhuang, C Wang, X Shi, S Qiu, S Zhang, B Xu, M Chen, W Jiang, H Dong, Y Qiao
    Int. J. Mol. Med., 2018;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  47. The fungal peptide toxin Candidalysin activates the NLRP3 inflammasome and causes cytolysis in mononuclear phagocytes
    Authors: L Kasper, A König, PA Koenig, MS Gresnigt, J Westman, RA Drummond, MS Lionakis, O Gro beta, J Ruland, JR Naglik, B Hube
    Nat Commun, 2018;9(1):4260.
  48. NLRP3 Inflammasome Formation and Activation in Nonalcoholic Steatohepatitis: Therapeutic Target for Antimetabolic Syndrome Remedy FTZ
    Authors: Y Chen, X He, X Yuan, J Hong, O Bhat, G Li, PL Li, J Guo
    Oxid Med Cell Longev, 2018;2018(0):2901871.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  49. Menthol, a unique urinary volatile compound, is associated with chronic inflammation in interstitial cystitis
    Authors: M Shahid, MY Lee, A Yeon, E Cho, V Sairam, L Valdiviez, S You, J Kim
    Sci Rep, 2018;8(1):10859.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  50. Caspase-11-mediated tubular epithelial pyroptosis underlies contrast-induced acute kidney injury
    Authors: Z Zhang, X Shao, N Jiang, S Mou, L Gu, S Li, Q Lin, Y He, M Zhang, W Zhou, Z Ni
    Cell Death Dis, 2018;9(10):983.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  51. Oridonin is a covalent NLRP3 inhibitor with strong anti-inflammasome activity
    Authors: H He, H Jiang, Y Chen, J Ye, A Wang, C Wang, Q Liu, G Liang, X Deng, W Jiang, R Zhou
    Nat Commun, 2018;9(1):2550.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  52. MPTP-driven NLRP3 inflammasome activation in microglia plays a central role in dopaminergic neurodegeneration
    Authors: E Lee, I Hwang, S Park, S Hong, B Hwang, Y Cho, J Son, JW Yu
    Cell Death Differ., 2018;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  53. LPS targets host guanylate-binding proteins to the bacterial outer membrane for non-canonical inflammasome activation
    Authors: JC Santos, MS Dick, B Lagrange, D Degrandi, K Pfeffer, M Yamamoto, E Meunier, P Pelczar, T Henry, P Broz
    EMBO J., 2018;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  54. REV-ERB? integrates colon clock with experimental colitis through regulation of NF-?B/NLRP3 axis
    Authors: S Wang, Y Lin, X Yuan, F Li, L Guo, B Wu
    Nat Commun, 2018;9(1):4246.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  55. HMGB1/IL-1? complexes in plasma microvesicles modulate immune responses to burn injury
    Authors: LG Coleman, R Maile, SW Jones, BA Cairns, FT Crews
    PLoS ONE, 2018;13(3):e0195335.
    Species: Mouse
    Sample Types: Protein
    Applications: Western Blot
  56. The oxidized phospholipid oxPAPC protects from septic shock by targeting the non-canonical inflammasome in macrophages
    Authors: LH Chu, M Indramohan, RA Ratsimandr, A Gangopadhy, EP Morris, DM Monack, A Dorfleutne, C Stehlik
    Nat Commun, 2018;9(1):996.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  57. Tranilast directly targets NLRP3 to treat inflammasome-driven diseases
    Authors: Y Huang, H Jiang, Y Chen, X Wang, Y Yang, J Tao, X Deng, G Liang, H Zhang, W Jiang, R Zhou
    EMBO Mol Med, 2018;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  58. Inhibitory Effect and Mechanism ofArctium lappaExtracton NLRP3Inflammasome Activation
    Authors: YK Kim, S Koppula, DW Shim, EJ In, SB Kwak, MK Kim, SH Yu, KH Lee, TB Kang
    Evid Based Complement Alternat Med, 2018;2018(0):6346734.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  59. Quercetin Inhibits Inflammasome Activation by Interfering with ASC Oligomerization and Prevents Interleukin-1 Mediated Mouse Vasculitis
    Authors: TP Domiciano, D Wakita, HD Jones, TR Crother, WA Verri, M Arditi, K Shimada
    Sci Rep, 2017;7(0):41539.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  60. Targeting IRE1 with small molecules counteracts progression of atherosclerosis
    Authors: O Tufanli, P Telkoparan, D Acosta-Alv, B Kocaturk, UI Onat, SM Hamid, I €imen, P Walter, C Weber, E Erbay
    Proc. Natl. Acad. Sci. U.S.A, 2017;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  61. Interleukin-1 and estrogen protect against disseminating dentoalveolar infections
    Authors: H Youssef, P Stashenko
    Int J Oral Sci, 2017;9(1):16-23.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  62. TLR sensing of bacterial spore-associated RNA triggers host immune responses with detrimental effects
    Authors: MK Choo, Y Sano, C Kim, K Yasuda, XD Li, X Lin, M Stenzel-Po, L Alexopoulo, S Ghosh, E Latz, IR Rifkin, ZJ Chen, GC Stewart, H Chong, JM Park
    J. Exp. Med., 2017;214(5):1297-1311.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  63. IL-1? transcriptionally activates hepcidin by inducing C/EBP? expression in hepatocytes
    Authors: Y Kanamori, M Murakami, M Sugiyama, O Hashimoto, T Matsui, M Funaba
    J. Biol. Chem., 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  64. MLKL, the Protein that Mediates Necroptosis, Also Regulates Endosomal Trafficking and Extracellular Vesicle Generation
    Authors: S Yoon, A Kovalenko, K Bogdanov, D Wallach
    Immunity, 2017;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  65. A new approach for ratiometric in vivo calcium imaging of microglia
    Authors: B Brawek, Y Liang, D Savitska, K Li, N Fomin-Thun, Y Kovalchuk, E Zirdum, J Jakobsson, O Garaschuk
    Sci Rep, 2017;7(1):6030.
  66. Myeloid-derived miR-223 regulates intestinal inflammation via repression of the NLRP3 inflammasome
    Authors: V Neudecker, M Haneklaus, O Jensen, L Khailova, JC Masterson, H Tye, K Biette, P Jedlicka, KS Brodsky, ME Gerich, M Mack, AAB Robertson, MA Cooper, GT Furuta, CA Dinarello, LA O'Neill, HK Eltzschig, SL Masters, EN McNamee
    J. Exp. Med., 2017;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  67. Distinct Kinase-Independent Role of RIPK3 in CD11c(+) Mononuclear Phagocytes in Cytokine-Induced Tissue Repair
    Authors: K Moriwaki, S Balaji, J Bertin, PJ Gough, FK Chan
    Cell Rep, 2017;18(10):2441-2451.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  68. AKT1 distinctively suppresses MyD88-depenedent and TRIF-dependent Toll-like receptor signaling in a kinase activity-independent manner
    Authors: K Zenke, M Muroi, KI Tanamoto
    Cell. Signal., 2017;43(0):32-39.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  69. The P2X7 Receptor Primes IL-1? and the NLRP3 Inflammasome in Astrocytes Exposed to Mechanical Strain
    Authors: F Albalawi, W Lu, JM Beckel, JC Lim, SA McCaughey, CH Mitchell
    Front Cell Neurosci, 2017;11(0):227.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  70. Nucleoside reverse transcriptase inhibitors (NRTIs) induce proinflammatory cytokines in the CNS via Wnt5a signaling
    Authors: T Wu, J Zhang, M Geng, SJ Tang, W Zhang, J Shu
    Sci Rep, 2017;7(1):4117.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  71. Tetrahydrobiopterin (BH4) deficiency is associated with augmented inflammation and microvascular degeneration in the retina
    Authors: JC Rivera, B Noueihed, A Madaan, I Lahaie, J Pan, J Belik, S Chemtob
    J Neuroinflammation, 2017;14(1):181.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  72. Papilloma-pseudovirus eradicates intestinal tumours and triples the lifespan of Apc(Min/+) mice
    Authors: Z Zhong, Y Zhai, P Bu, S Shah, L Qiao
    Nat Commun, 2017;8(0):15004.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  73. The intracellular chloride channel proteins CLIC1 and CLIC4 induce IL-1? transcription and activate the NLRP3 inflammasome
    Authors: R Domingo-Fe, RC Coll, J Kearney, S Breit, LA O'Neill
    J. Biol. Chem., 2017;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  74. SR-B1 Is a Silica Receptor that Mediates Canonical Inflammasome Activation
    Authors: M Tsugita, N Morimoto, M Tashiro, K Kinoshita, M Nakayama
    Cell Rep, 2017;18(5):1298-1311.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  75. NLR members NLRC4 and NLRP3 mediate sterile inflammasome activation in microglia and astrocytes
    Authors: L Freeman, H Guo, CN David, WJ Brickey, S Jha, JP Ting
    J. Exp. Med., 2017;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  76. Cutting Edge: Distinct Regulatory Mechanisms Control Proinflammatory Cytokines IL-18 and IL-1?
    Authors: Q Zhu, TD Kanneganti
    J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  77. Toxoplasma gondii GRA7-Targeted ASC and PLD1 Promote Antibacterial Host Defense via PKC�
    Authors: HJ Koh, YR Kim, JS Kim, JS Yun, K Jang, CS Yang
    PLoS Pathog, 2017;13(1):e1006126.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  78. MTG16 is a tumor suppressor in colitis-associated carcinoma
    Authors: EM McDonough, CW Barrett, B Parang, MK Mittal, JJ Smith, AM Bradley, YA Choksi, LA Coburn, SP Short, JJ Thompson, B Zhang, SV Poindexter, MA Fischer, X Chen, J Li, FL Revetta, R Naik, MK Washington, MJ Rosen, SW Hiebert, KT Wilson, CS Williams
    JCI Insight, 2017;2(16):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  79. The PYHIN Protein p205 Regulates the Inflammasome by Controlling Asc Expression
    Authors: S Ghosh, C Wallerath, S Covarrubia, V Hornung, S Carpenter, KA Fitzgerald
    J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  80. Lentinan from shiitake selectively attenuates AIM2 and non-canonical inflammasome activation while inducing pro-inflammatory cytokine production
    Authors: H Ahn, E Jeon, JC Kim, SG Kang, SI Yoon, HJ Ko, PH Kim, GS Lee
    Sci Rep, 2017;7(1):1314.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  81. Activation of NLRP3 inflammasomes in mouse hepatic stellate cells during Schistosoma J. infection
    Oncotarget, 2016;7(26):39316-39331.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  82. Histone deacetylase inhibitors mediate DNA damage repair in ameliorating hemorrhagic cystitis
    Sci Rep, 2016;6(0):39257.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  83. Pyrin inflammasome activation and RhoA signaling in the autoinflammatory diseases FMF and HIDS
    Authors: Yong Hwan Park
    Nat Immunol, 2016;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  84. Functional and Evolutionary Analyses Identify Proteolysis as a General Mechanism for NLRP1 Inflammasome Activation
    PLoS Pathog, 2016;12(12):e1006052.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  85. Enzymatically inactive procaspase-1 stabilizes the ASC-pyroptosome and supports pyroptosome spreading during cell division
    J Biol Chem, 2016;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Bead-based Immunoassay
  86. Fluoxetine Inhibits NLRP3 Inflammasome Activation: Implication in Depression
    Authors: Gang Hu
    Int. J. Neuropsychopharmacol., 2016;19(9):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  87. CD8+ T cells produce a dialyzable antigen-specific activator of dendritic cells
    J Leukoc Biol, 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  88. HIF-1? Is an Essential Mediator of IFN-?-Dependent Immunity to Mycobacterium tuberculosis
    J Immunol, 2016;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  89. CARD9 negatively regulates NLRP3-induced IL-1? production on Salmonella infection of macrophages
    Nat Commun, 2016;7(0):12874.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  90. C13orf31 (FAMIN) is a central regulator of immunometabolic function
    Nat Immunol, 2016;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  91. TNF?-stimulated gene-6 (TSG6) activates macrophage phenotype transition to prevent inflammatory lung injury
    Proc. Natl. Acad. Sci. U.S.A., 2016;113(50):E8151-E8158.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  92. Pericyte MyD88 and IRAK4 control inflammatory and fibrotic responses to tissue injury
    Authors: Jeremy S Duffield
    J. Clin. Invest., 2016;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  93. Lymphatic vessels regulate immune microenvironments in human and murine melanoma
    J Clin Invest, 2016;0(0):.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  94. Identification of QS-21 as an Inflammasome-activating Molecular Component of Saponin Adjuvants.
    Authors: Marty-Roix R, Vladimer G, Pouliot K, Weng D, Buglione-Corbett R, West K, Macmicking J, Chee J, Wang S, Lu S, Lien E
    J Biol Chem, 2016;291(3):1123-36.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  95. The Tick Protein Sialostatin L2 Binds to Annexin A2 and Inhibits NLRC4-Mediated Inflammasome Activation
    Authors: X Wang, DK Shaw, OS Sakhon, GA Snyder, EJ Sundberg, L Santambrog, FS Sutterwala, JS Dumler, KA Shirey, DJ Perkins, K Richard, AC Chagas, E Calvo, J Kopecký, M Kotsyfakis, JH Pedra
    Infect Immun, 2016;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  96. Retinal neurons curb inflammation and enhance revascularization in ischemic retinopathies via proteinase-activated receptor-2.
    Authors: Sitaras N, Rivera J, Noueihed B, Bien-Aime M, Zaniolo K, Omri S, Hamel D, Zhu T, Hardy P, Sapieha P, Joyal J, Chemtob S
    Am J Pathol, 2015;185(2):581-95.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  97. Deficient NLRP3 and AIM2 Inflammasome Function in Autoimmune NZB Mice.
    Authors: Sester D, Sagulenko V, Thygesen S, Cridland J, Loi Y, Cridland S, Masters S, Genske U, Hornung V, Andoniou C, Sweet M, Degli-Esposti M, Schroder K, Stacey K
    J Immunol, 2015;195(3):1233-41.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  98. Guanylate-binding proteins promote activation of the AIM2 inflammasome during infection with Francisella novicida.
    Authors: Meunier E, Wallet P, Dreier R, Costanzo S, Anton L, Ruhl S, Dussurgey S, Dick M, Kistner A, Rigard M, Degrandi D, Pfeffer K, Yamamoto M, Henry T, Broz P
    , 2015;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  99. RIPK3 promotes cell death and NLRP3 inflammasome activation in the absence of MLKL.
    Authors: Lawlor K, Khan N, Mildenhall A, Gerlic M, Croker B, D'Cruz A, Hall C, Kaur Spall S, Anderton H, Masters S, Rashidi M, Wicks I, Alexander W, Mitsuuchi Y, Benetatos C, Condon S, Wong W, Silke J, Vaux D, Vince J
    Nat Commun, 2015;6(0):6282.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  100. Caspase-1-independent IL-1 release mediates blister formation in autoantibody-induced tissue injury through modulation of endothelial adhesion molecules.
    Authors: Sadeghi H, Lockmann A, Hund A, Samavedam U, Pipi E, Vafia K, Hauenschild E, Kalies K, Pas H, Jonkman M, Iwata H, Recke A, Schon M, Zillikens D, Schmidt E, Ludwig R
    J Immunol, 2015;194(8):3656-63.
  101. The cyclopentenone prostaglandin 15d-PGJ2 inhibits the NLRP1 and NLRP3 inflammasomes.
    Authors: Maier N, Leppla S, Moayeri M
    J Immunol, 2015;194(6):2776-85.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  102. Methylsulfonylmethane inhibits NLRP3 inflammasome activation.
    Authors: Ahn H, Kim J, Lee M, Kim Y, Cho Y, Lee G
    Cytokine, 2015;71(2):223-31.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  103. AIM2 and NLRC4 inflammasomes contribute with ASC to acute brain injury independently of NLRP3.
    Authors: Denes A, Coutts G, Lenart N, Cruickshank S, Pelegrin P, Skinner J, Rothwell N, Allan S, Brough D
    Proc Natl Acad Sci U S A, 2015;112(13):4050-5.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  104. Small heterodimer partner interacts with NLRP3 and negatively regulates activation of the NLRP3 inflammasome.
    Authors: Yang C, Kim J, Kim T, Lee P, Kim S, Lee H, Shin D, Nguyen L, Lee M, Jin H, Kim K, Lee C, Kim M, Park S, Kim J, Choi H, Jo E
    Nat Commun, 2015;6(0):6115.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  105. Local immunosuppressive microenvironment enhances migration of melanoma cells to lungs in DJ-1 knockout mice.
    Authors: Chien C, Lee M, Liou H, Liou H, Fu W
    PLoS ONE, 2015;10(2):e0115827.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  106. IL-10 engages macrophages to shift Th17 cytokine dependency and pathogenicity during T-cell-mediated colitis.
    Authors: Li B, Gurung P, Malireddi R, Vogel P, Kanneganti T, Geiger T
    Nat Commun, 2015;6(0):6131.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  107. The frustrated host response to Legionella pneumophila is bypassed by MyD88-dependent translation of pro-inflammatory cytokines.
    Authors: Asrat S, Dugan A, Isberg R
    PLoS Pathog, 2014;10(7):e1004229.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  108. RNA and beta-hemolysin of group B Streptococcus induce interleukin-1beta (IL-1beta) by activating NLRP3 inflammasomes in mouse macrophages.
    Authors: Gupta R, Ghosh S, Monks B, DeOliveira R, Tzeng T, Kalantari P, Nandy A, Bhattacharjee B, Chan J, Ferreira F, Rathinam V, Sharma S, Lien E, Silverman N, Fitzgerald K, Firon A, Trieu-Cuot P, Henneke P, Golenbock D
    J Biol Chem, 2014;289(20):13701-5.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  109. A critical role for human caspase-4 in endotoxin sensitivity.
    Authors: Kajiwara Y, Schiff T, Voloudakis G, Gama Sosa M, Elder G, Bozdagi O, Buxbaum J
    J Immunol, 2014;193(1):335-43.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  110. Caspase-8 modulates dectin-1 and complement receptor 3-driven IL-1beta production in response to beta-glucans and the fungal pathogen, Candida albicans.
    Authors: Ganesan S, Rathinam V, Bossaller L, Army K, Kaiser W, Mocarski E, Dillon C, Green D, Mayadas T, Levitz S, Hise A, Silverman N, Fitzgerald K
    J Immunol, 2014;193(5):2519-30.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  111. Nuclear factor E2-related factor-2 (Nrf2) is required for NLRP3 and AIM2 inflammasome activation.
    Authors: Zhao C, Gillette D, Li X, Zhang Z, Wen H
    J Biol Chem, 2014;289(24):17020-9.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  112. Endothelial NLRP3 inflammasome activation and enhanced neointima formation in mice by adipokine visfatin.
    Authors: Xia M, Boini K, Abais J, Xu M, Zhang Y, Li P
    Am J Pathol, 2014;184(5):1617-28.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  113. Severe acute respiratory syndrome coronavirus envelope protein ion channel activity promotes virus fitness and pathogenesis.
    Authors: Nieto-Torres J, DeDiego M, Verdia-Baguena C, Jimenez-Guardeno J, Regla-Nava J, Fernandez-Delgado R, Castano-Rodriguez C, Alcaraz A, Torres J, Aguilella V, Enjuanes L
    PLoS Pathog, 2014;10(5):e1004077.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  114. Systemic autophagy insufficiency compromises adaptation to metabolic stress and facilitates progression from obesity to diabetes.
    Authors: Lim, Yu-Mi, Lim, Hyejin, Hur, Kyu Yeon, Quan, Wenying, Lee, Hae-Youn, Cheon, Hwanju, Ryu, Dongryeo, Koo, Seung-Ho, Kim, Hong Lim, Kim, Jin, Komatsu, Masaaki, Lee, Myung-Sh
    Nat Commun, 2014;5(0):4934.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  115. Critical role of aquaporins in interleukin 1beta (IL-1beta)-induced inflammation.
    Authors: Rabolli V, Wallemme L, Lo Re S, Uwambayinema F, Palmai-Pallag M, Thomassen L, Tyteca D, Octave J, Marbaix E, Lison D, Devuyst O, Huaux F
    J Biol Chem, 2014;289(20):13937-47.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  116. Critical role for IL-1beta in DNA damage-induced mucositis.
    Authors: Kanarek N, Grivennikov S, Leshets M, Lasry A, Alkalay I, Horwitz E, Shaul Y, Stachler M, Voronov E, Apte R, Pagano M, Pikarsky E, Karin M, Ghosh S, Ben-Neriah Y
    Proc Natl Acad Sci U S A, 2014;111(6):E702-11.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  117. Activation of NLRP3 inflammasome by crystalline structures via cell surface contact.
    Authors: Hari A, Zhang Y, Tu Z, Detampel P, Stenner M, Ganguly A, Shi Y
    Sci Rep, 2014;4(0):7281.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  118. Arsenic trioxide and other arsenical compounds inhibit the NLRP1, NLRP3, and NAIP5/NLRC4 inflammasomes.
    Authors: Maier N, Crown D, Liu J, Leppla S, Moayeri M
    J Immunol, 2014;192(2):763-70.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  119. 3,4-methylenedioxy-beta-nitrostyrene inhibits NLRP3 inflammasome activation by blocking assembly of the inflammasome.
    Authors: He Y, Varadarajan S, Munoz-Planillo R, Burberry A, Nakamura Y, Nunez G
    J Biol Chem, 2014;289(2):1142-50.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  120. Caspase-11 activation requires lysis of pathogen-containing vacuoles by IFN-induced GTPases.
    Authors: Meunier E, Dick M, Dreier R, Schurmann N, Kenzelmann Broz D, Warming S, Roose-Girma M, Bumann D, Kayagaki N, Takeda K, Yamamoto M, Broz P
    Nature, 2014;509(7500):366-70.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  121. Signaling pathways in murine dendritic cells that regulate the response to vesicular stomatitis virus vectors that express flagellin.
    Authors: Smedberg J, Westcott M, Ahmed M, Lyles D
    J Virol, 2014;88(2):777-85.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  122. Wingless-type mammary tumor virus integration site family, member 5A (Wnt5a) regulates human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein 120 (gp120)-induced expression of pro-inflammatory cytokines via the Ca2+/calmodulin-dependent protein kinase II (CaMKII) and c-Jun N-terminal kinase (JNK) signaling pathways.
    Authors: Li B, Shi Y, Shu J, Gao J, Wu P, Tang S
    J Biol Chem, 2013;288(19):13610-9.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  123. Microtubule-driven spatial arrangement of mitochondria promotes activation of the NLRP3 inflammasome.
    Authors: Misawa T, Takahama M, Kozaki T, Lee H, Zou J, Saitoh T, Akira S
    Nat Immunol, 2013;14(5):454-60.
    Species: Mouse
    Sample Types: Peritoneal Fluid
    Applications: Western Blot
  124. Gli1 deletion prevents Helicobacter-induced gastric metaplasia and expansion of myeloid cell subsets.
    Authors: El-Zaatari M, Kao J, Tessier A, Bai L, Hayes M, Fontaine C, Eaton K, Merchant J
    PLoS ONE, 2013;8(3):e58935.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  125. The role of IL-1beta in the early tumor cell-induced angiogenic response.
    Authors: Carmi Y, Dotan S, Rider P, Kaplanov I, White M, Baron R, Abutbul S, Huszar M, Dinarello C, Apte R, Voronov E
    J Immunol, 2013;190(7):3500-9.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  126. Activation of microglia induces symptoms of Parkinson's disease in wild-type, but not in IL-1 knockout mice.
    Authors: Tanaka S, Ishii A, Ohtaki H, Shioda S, Yoshida T, Numazawa S
    J Neuroinflammation, 2013;10(0):143.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  127. The calcium-sensing receptor regulates the NLRP3 inflammasome through Ca2+ and cAMP.
    Authors: Lee G, Subramanian N, Kim A, Aksentijevich I, Goldbach-Mansky R, Sacks D, Germain R, Kastner D, Chae J
    Nature, 2012;492(7427):123-7.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  128. Caspase-11 increases susceptibility to Salmonella infection in the absence of caspase-1.
    Authors: Broz P, Ruby T, Belhocine K, Bouley D, Kayagaki N, Dixit V, Monack D
    Nature, 2012;490(7419):288-91.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  129. Dectin-1-dependent interleukin-22 contributes to early innate lung defense against Aspergillus fumigatus.
    Authors: Gessner MA, Werner JL, Lilly LM, Nelson MP, Metz AE, Dunaway CW, Chan YR, Ouyang W, Brown GD, Weaver CT, Steele C
    Infect. Immun., 2012;80(1):410-7.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  130. Toll-like receptor 2 contributes to chemokine gene expression and macrophage infiltration in the dorsal root ganglia after peripheral nerve injury.
    Authors: Kim D, You B, Lim H, Lee SJ
    Mol Pain, 2011;7(0):74.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  131. IL-1alpha and IL-1beta recruit different myeloid cells and promote different stages of sterile inflammation.
    Authors: Rider P, Carmi Y, Guttman O, Braiman A, Cohen I, Voronov E, White MR, Dinarello CA, Apte RN
    J. Immunol., 2011;187(9):4835-43.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  132. IL-6 trans-signaling licenses mouse and human tumor microvascular gateways for trafficking of cytotoxic T cells.
    Authors: Fisher DT, Chen Q, Skitzki JJ, Muhitch JB, Zhou L, Appenheimer MM, Vardam TD, Weis EL, Passanese J, Wang WC, Gollnick SO, Dewhirst MW, Rose-John S, Repasky EA, Baumann H, Evans SS
    J. Clin. Invest., 2011;121(10):3846-59.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  133. Coordinated Host Responses during Pyroptosis: Caspase-1-Dependent Lysosome Exocytosis and Inflammatory Cytokine Maturation.
    Authors: Bergsbaken T, Fink SL, den Hartigh AB, Loomis WP, Cookson BT
    J. Immunol., 2011;187(5):2748-54.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  134. Microenvironment-derived IL-1 and IL-17 interact in the control of lung metastasis.
    Authors: Carmi Y, Rinott G, Dotan S, Elkabets M, Rider P, Voronov E, Apte RN
    J. Immunol., 2011;186(6):3462-71.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  135. Interleukin-1beta mediates the extra-intestinal thrombosis associated with experimental colitis.
    Authors: Yoshida H, Russell J, Senchenkova EY, Almeida Paula LD, Granger DN
    Am. J. Pathol., 2010;177(6):2774-81.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  136. Cytotoxins of the human pathogen Aeromonas hydrophila trigger, via the NLRP3 inflammasome, caspase-1 activation in macrophages.
    Authors: McCoy AJ, Koizumi Y, Toma C, Higa N, Dixit V, Taniguchi S, Tschopp J, Suzuki T
    Eur. J. Immunol., 2010;40(10):2797-803.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  137. Activation of the NLRP3 inflammasome by islet amyloid polypeptide provides a mechanism for enhanced IL-1beta in type 2 diabetes.
    Authors: Masters SL, Dunne A, Subramanian SL, Hull RL, Tannahill GM, Sharp FA, Becker C, Franchi L, Yoshihara E, Chen Z, Mullooly N, Mielke LA, Harris J, Coll RC, Mills KH, Mok KH, Newsholme P, Nunez G, Yodoi J, Kahn SE, Lavelle EC, O'Neill LA
    Nat. Immunol., 2010;11(10):897-904.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  138. Tyrosine kinase 2 controls IL-1beta production at the translational level.
    Authors: Radwan M, Stiefvater R, Grunert T, Sharif O, Miller I, Marchetti-Deschmann M, Allmaier G, Gemeiner M, Knapp S, Kovarik P, Muller M, Strobl B
    J. Immunol., 2010;185(6):3544-53.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  139. Externalization of the leaderless cytokine IL-1F6 occurs in response to lipopolysaccharide/ATP activation of transduced bone marrow macrophages.
    Authors: Martin U, Scholler J, Gurgel J, Renshaw B, Sims JE, Gabel CA
    J. Immunol., 2009;183(6):4021-30.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  140. Activation of the NLRP3 inflammasome in dendritic cells induces IL-1beta-dependent adaptive immunity against tumors.
    Authors: Ghiringhelli F, Apetoh L, Tesniere A, Aymeric L, Ma Y, Ortiz C, Vermaelen K, Panaretakis T, Mignot G, Ullrich E, Perfettini JL, Schlemmer F, Tasdemir E, Uhl M, Genin P, Civas A, Ryffel B, Kanellopoulos J, Tschopp J, Andre F, Lidereau R, McLaughlin NM, Haynes NM, Smyth MJ, Kroemer G, Zitvogel L
    Nat. Med., 2009;15(10):1170-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  141. Chlamydial and periodontal pathogens induce hepatic inflammation and fatty acid imbalance in apolipoprotein E-deficient mice.
    Authors: Hyvarinen K, Tuomainen AM, Laitinen S, Bykov IL, Tormakangas L, Lindros K, Käkelä R, Alfthan G, Salminen I, Jauhiainen M, Kovanen PT, Leinonen M, Saikku P, Pussinen PJ
    Infect. Immun., 2009;77(8):3442-9.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  142. Japanese Encephalitis Virus infection induces IL-18 and IL-1beta in microglia and astrocytes: correlation with in vitro cytokine responsiveness of glial cells and subsequent neuronal death.
    Authors: Das S, Mishra MK, Ghosh J, Basu A
    J. Neuroimmunol., 2008;195(1):60-72.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  143. Stimulation of Toll-like receptor 3 and 4 induces interleukin-1beta maturation by caspase-8.
    Authors: Maelfait J, Vercammen E, Janssens S, Schotte P, Haegman M, Magez S, Beyaert R
    J. Exp. Med., 2008;205(9):1967-73.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  144. Toll-like receptor 4 (TLR4)-dependent proinflammatory and immunomodulatory properties of the glycoinositolphospholipid (GIPL) from Trypanosoma cruzi.
    Authors: Medeiros MM, Peixoto JR, Oliveira AC, Cardilo-Reis L, Koatz VL, Van Kaer L, Previato JO, Mendonca-Previato L, Nobrega A, Bellio M
    J. Leukoc. Biol., 2007;82(3):488-96.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  145. Granulocyte colony-stimulating factor impairs liver regeneration in mice through the up-regulation of interleukin-1beta.
    Authors: Ogiso T, Nagaki M, Takai S, Tsukada Y, Mukai T, Kimura K, Moriwaki H
    J. Hepatol., 2007;47(6):816-25.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  146. Topical acetone treatment induces neurogenic oedema on the sensitized mouse ear: an in vivo study using transient receptor potential vanilloid 1 (TRPV1) receptor knockout mice.
    Authors: Pozsgai G, Sandor K, Perkecz A, Szolcsanyi J, Helyes Z, Brain SD, Pinter E
    Inflamm. Res., 2007;56(11):459-67.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  147. Inflammasome-mediated production of IL-1beta is required for neutrophil recruitment against Staphylococcus aureus in vivo.
    Authors: Miller LS, Pietras EM, Uricchio LH, Hirano K, Rao S, Lin H, O'Connell RM, Iwakura Y, Cheung AL, Cheng G, Modlin RL
    J. Immunol., 2007;179(10):6933-42.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  148. The Myc-dependent angiogenic switch in tumors is mediated by interleukin 1beta.
    Authors: Shchors K, Shchors E, Rostker F, Lawlor ER, Brown-Swigart L, Evan GI
    Genes Dev., 2006;20(18):2527-38.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  149. Involvement of monocyte chemoattractant protein-1, macrophage inflammatory protein-1alpha and interleukin-1beta in Wallerian degeneration.
    Authors: Perrin FE, Lacroix S, Aviles-Trigueros M, David S
    Brain, 2005;128(0):854-66.
    Species: Mouse
    Sample Types:
    Applications: Neutralization
  150. Intravenous human interleukin-1alpha impairs memory processing in mice: dependence on blood-brain barrier transport into posterior division of the septum.
    Authors: Banks WA, Farr SA, La Scola ME
    J. Pharmacol. Exp. Ther., 2001;299(2):536-41.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  151. Biglycan, a danger signal that activates the NLRP3 inflammasome via toll-like and P2X receptors.
    Authors: Babelova A, Moreth K, Tsalastra-Greul W, Zeng-Brouwers J, Eickelberg O, Young MF, Bruckner P, Pfeilschifter J, Schaefer RM, Schaefer R, Grone HJ, Schaefer L
    J Biol Chem, 0;284(36):24035-48.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P

FAQs

  1. Does catalog # AF-401-NA detect the pro or mature form of IL-1 beta in western blot?

    • This would depend on the sample being used. The pro-form of the protein also contains the sequence for the mature form.  When cell lysates are used as the sample, the pro-form is mainly detected.  When cell culture supernatants containing mainly the mature form of the protein is used, only the mature form is detected.  This is exemplified in the following paper:

      J Immunol.(2015) 195, 1233.  http://www.jimmunol.org/content/195/3/1233/tab-figures-data

View all Antibody FAQs

Cell and Tissue Staining Kits

Anti-Goat HRP-DAB Cell & Tissue Staining Kit

CTS008
22 Citations
6 Reviews

Immunohistochemistry Reagents

Antigen Retrieval Reagent Sampler (50 mL each)

CTS016
3 Citations

Antigen Retrieval Reagent-Acidic

CTS014
2 Citations

Antigen Retrieval Reagent-Basic

CTS013
8 Citations

Antigen Retrieval Reagent-Universal

CTS015
13 Citations

VisUCyte Antigen Retrieval Reagent-Acidic

VCTS022

VisUCyte Antigen Retrieval Reagent-Basic

VCTS021

VisUCyte Antigen Retrieval Reagent-Universal

VCTS023

Isotype Controls

Normal Goat IgG Control

AB-108-C
256 Citations
16 Reviews
1 Image
Ctrl

Reconstitution Buffers

Reconstitution Buffer 1 (PBS)

RB01
2 Reviews

Secondary Antibodies

Chicken Anti-Goat IgG Biotinylated Antibody

BAF019
WB

Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

F0124
Flow

Donkey Anti-Goat IgG Antibody

AF109
6 Citations
WB

Donkey Anti-Goat IgG Biotinylated Antibody

BAF109
3 Citations
1 Review
WB

Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

NL003
7 Citations
Flow,IHC,ICC

Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

NL001
12 Citations
1 Review
Flow,IHC,ICC

Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

NL002
1 Citations
Flow,IHC,ICC

Goat IgG (H+L) APC-conjugated Antibody

F0108
9 Citations
1 Image
Flow

Goat IgG (H+L) Fluorescein-conjugated Antibody

F0109
2 Citations
1 Image
Flow

Goat IgG (H+L) PE-conjugated Antibody

F0107
5 Citations
1 Image
Flow

Goat IgG HRP-conjugated Antibody

HAF017
25 Citations
8 Reviews
1 Image
WB

Goat IgG HRP-conjugated Antibody

HAF109
32 Citations
3 Reviews
2 Images
WB,Simple Western

Goat IgG Horseradish Peroxidase-conjugated Antibody

HAF019
10 Citations
1 Image
WB

Goat IgG VisUCyte HRP Polymer

VC004
2 Citations
1 Image
IHC

Rabbit Anti-Goat IgG (H+L) Antibody, X Absorbed

R-401-C-ABS
WB,ELISA

Rabbit Anti-Goat IgG Biotinylated Antibody

BAF017
1 Citations
1 Review
WB

Reviews for Mouse IL-1 beta /IL-1F2 Antibody

Average Rating: 3.9 (Based on 16 Reviews)

5 Star
18.75%
4 Star
62.5%
3 Star
12.5%
2 Star
0%
1 Star
6.25%

Have you used Mouse IL-1 beta /IL-1F2 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Mouse IL-1 beta /IL-1F2 Antibody
By Anonymous on 11/23/2020
Application: IHC Sample Tested: mouse oral tissue Species: Mouse
Immunohistochemistry Mouse IL-1 beta /IL-1F2 Antibody AF-401-NA
Mouse IL-1 beta /IL-1F2 Antibody
By Anonymous on 11/19/2020
Application: WB Sample Tested: THP-1 human acute monocytic leukemia cell line Species: Human

Human-derived THP-1 cells either left unstimulated or treated with LPS+ATP for 3 hours. Intracellular detection.

Western Blot Mouse IL-1 beta /IL-1F2 Antibody AF-401-NA
Mouse IL-1 beta /IL-1F2 Antibody
By Anonymous on 05/23/2019
Application: WB Sample Tested: Brain (caudate putamen) Species: Mouse

I am somewhat disappointed. My lab published 4 years ago an article using this Ab. I boight it last september.
There is no way I can get the bands ourselves and other authors have published: the proIL1-beta and the mature form, at around 35 and 17kDa.
Attached is a representative blot of the many I have run.
I have tried homemade 12% gels, BioRad 12 and 4-20% precast gels. I have tried both types of membranes (PVDF and Nitro 0.2), blocking with milk and BSA, different transfer times (35min 120V, 45min 100V, 1h 100V, 1.5h 100V), different Ab dilutions. And I still get nothing that comes to close to the pro-IL-1beta, and of course, the mature form is nowhere to be found.
I do not wish to buy another brand, I believe this to be a good Ab, but since it is polyclonal, it might give different results depending on the lot. ***RnD Systems Response: Thank you for reviewing our product. We are sorry to that that this antibody did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.***

Western Blot Mouse IL-1 beta /IL-1F2 Antibody AF-401-NA
Mouse IL-1 beta /IL-1F2 Antibody
By Anonymous on 01/28/2019
Application: WB Sample Tested: IC-21 mouse macrophage cell line Species: Mouse
Western Blot Mouse IL-1 beta /IL-1F2 Antibody AF-401-NA
Mouse IL-1 beta /IL-1F2 Antibody
By Anonymous on 01/28/2019
Application: WB Sample Tested: IC-21 mouse macrophage cell line Species: Mouse
Mouse IL-1 beta /IL-1F2 Antibody
By Anonymous on 08/08/2018
Application: IHC Sample Tested: Brain tissue Species: Mouse

10.1093/cercor/bhy164

Immunohistochemistry Mouse IL-1 beta /IL-1F2 Antibody AF-401-NA
Mouse IL-1 beta /IL-1F2 Antibody
By Anonymous on 07/17/2018
Application: Simple Western Sample Tested: J774A.1 mouse reticulum cell sarcoma macrophage cell line Species: Mouse
Simple Western Mouse IL-1 beta /IL-1F2 Antibody AF-401-NA
Mouse IL-1 beta /IL-1F2 Antibody
By Anonymous on 07/17/2018
Application: WB Sample Tested: Macrophages Cell Supernatant,Cell Lysates Species: Mouse macrophages,Mouse
Western Blot Mouse IL-1 beta /IL-1F2 Antibody AF-401-NA
Mouse IL-1 beta /IL-1F2 Antibody
By Anonymous on 07/17/2018
Application: WB Sample Tested: Cell Lysates Species: Mouse
Western Blot Mouse IL-1 beta /IL-1F2 Antibody AF-401-NA
Mouse IL-1 beta /IL-1F2 Antibody
By Anonymous on 05/17/2018
Application: ELISA Sample Tested: A549 human lung carcinoma cell line Species: Human
Mouse IL-1 beta /IL-1F2 Antibody
By Anonymous on 03/22/2018
Application: WB Sample Tested: IC-21 mouse macrophage cell line Species: Mouse
Mouse IL-1 beta /IL-1F2 Antibody
By Anonymous on 10/31/2017
Application: WB Sample Tested: bone marrow derived macrophage Species: Mouse
Western Blot Mouse IL-1 beta /IL-1F2 Antibody AF-401-NA
Mouse IL-1 beta /IL-1F2 Antibody
By Anonymous on 09/08/2017
Application: Flow Sample Tested: L-929 mouse fibroblast cell line Species: Mouse
Mouse IL-1 beta /IL-1F2 Antibody
By Anonymous on 04/26/2017
Application: B/N Sample Tested: Adult splenocytes Species: Mouse
Mouse IL-1 beta /IL-1F2 Antibody
By Anonymous on 04/25/2017
Application: WB Sample Tested: Cell culture supernatant,Serum-free Cell Culture Supernates,Bone marrow cells Species: Mouse
Western Blot Mouse IL-1 beta /IL-1F2 Antibody AF-401-NA
Mouse IL-1 beta /IL-1F2 Antibody
By Anonymous on 02/14/2017
Application: WB Sample Tested: bone marrow derived macrophages Species: Mouse
Western Blot Mouse IL-1 beta /IL-1F2 Antibody AF-401-NA