Mouse IL-1 beta /IL-1F2 Antibody

Catalog # Availability Size / Price Qty
MAB401-SP
MAB401-500
MAB401-100
Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutral­ization by Mouse IL‑1 beta /IL‑1F2 Antibody.
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Product Details
Citations (25)
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Reviews (1)

Mouse IL-1 beta /IL-1F2 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-1 beta /IL-1F2 in ELISAs.
Source
Monoclonal Rat IgG1 Clone # 30311
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse IL-1 beta /IL-1F2
Val118-Ser269
Accession # P10749
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample

Mouse IL-1 beta /IL-1F2 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Mouse IL‑1 beta /IL‑1F2 Biotinylated Antibody (Catalog # BAF401)

Standard: Recombinant Mouse IL-1 beta/IL-1F2 Protein (Catalog # 401-ML)

Neutralization
Measured by its ability to neutralize IL‑1 beta /IL‑1F2-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND50) is typically 1-5 µg/mL in the presence of 50 pg/mL Recombinant Mouse IL‑1 beta /IL‑1F2 and 1.25 µg/mL concanavalin A.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutral­ization by Mouse IL‑1 beta /IL‑1F2 Antibody. View Larger

Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutral­ization by Mouse IL‑1 beta /IL‑1F2 Antibody. Recombinant Mouse IL‑1 beta /IL‑1F2 (Catalog # 401-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑1 beta /IL‑1F2 (50 pg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB401). The ND50 is typically 1-5 µg/mL in the presence of concanavalin A (1.25 µg/mL).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-1 beta/IL-1F2

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 17% amino acid (aa) identity in mouse. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1-4). The mouse IL-1 beta cDNA encodes a 269 aa precursor. A 117 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5, 6). The 17 kDa mature mouse IL-1 beta shares 90% aa sequence identity with cotton rat and rat and 65-78% identity with canine, equine, feline, human, porcine, and rhesus macaque IL‑1 beta.

References
  1. Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629.
  2. Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
  3. Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S.
  4. Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21.
  5. Gray, P.W. et al. (1986) J. Immunol. 137:3644.
  6. Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.
Long Name
Interleukin 1 beta
Entrez Gene IDs
3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
Alternate Names
catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta

Product Datasheets

Citations for Mouse IL-1 beta /IL-1F2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

25 Citations: Showing 1 - 10
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  1. Inhibitory effect ofquercetinon titaniumparticle-inducedendoplasmic reticulum stress (ERS)-related apoptosisandin vivoosteolysis
    Authors: L Zhang, Z Tian, W Li, X Wang, Z Man, S Sun
    Biosci. Rep., 2017;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Capture
  2. The calcineurin-NFAT axis contributes to host defense during Pseudomonas aeruginosa lung infection
    Authors: Z Pang, RD Junkins, AJ MacNeil, C McCormick, Z Cheng, WM Chen, TJ Lin
    J. Leukoc. Biol., 2017;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Capture
  3. Anti-inflammatory effects of a traditional Korean medicine: Ojayeonjonghwan
    Authors: SY Nam, KY Kim, MH Kim, JB Jang, SY Rah, HJ Chae, JM Lee, HM Kim, HJ Jeong
    Pharm Biol, 2017;55(1):1856-1862.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Capture
  4. Mucin 1 protects against severe Streptococcus pneumoniae infection
    Authors: P Dhar, GZ Ng, EM Dunne, P Sutton
    Virulence, 2017;0(0):0.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Capture
  5. Anti-inflammatory Effects of the Natural Compounds Cortex Phellodendri and Humulus japonicus on Pelvic Inflammatory Disease in Mice
    Authors: Y Oh, YS Kwon, BD Jung
    Int J Med Sci, 2017;14(8):729-734.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Capture
  6. Oryza sativa (Rice) Hull Extract Inhibits Lipopolysaccharide-Induced Inflammatory Response in RAW264.7 Macrophages by Suppressing Extracellular Signal-regulated Kinase, c-Jun N-terminal Kinase, and Nuclear Factor-?B Activation
    Authors: Yoonsook Kim
    Pharmacogn Mag, 2016;12(48):295-301.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Capture
  7. Inhibiting the NLRP3 inflammasome with MCC950 promotes non-phlogistic clearance of amyloid-? and cognitive function in APP/PS1 mice
    Authors: C Dempsey
    Brain Behav. Immun, 2016;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Capture
  8. Myeloid cell TRAF3 regulates immune responses and inhibits inflammation and tumor development in mice.
    Authors: Lalani A, Moore C, Luo C, Kreider B, Liu Y, Morse H, Xie P
    J Immunol, 2015;194(1):334-48.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Development
  9. AMCase is a crucial regulator of type 2 immune responses to inhaled house dust mites.
    Authors: Kim L, Morita R, Kobayashi Y, Eisenbarth S, Lee C, Elias J, Eynon E, Flavell R
    Proc Natl Acad Sci U S A, 2015;112(22):E2891-9.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Capture
  10. Glimepiride reduces CD14 expression and cytokine secretion from macrophages.
    Authors: Ingham V, Williams A, Bate C
    J Neuroinflammation, 2014;11(0):115.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Capture
  11. Multiple CD11c+ cells collaboratively express IL-1beta to modulate stromal vascular endothelial growth factor and lymph node vascular-stromal growth.
    Authors: Benahmed F, Chyou S, Dasoveanu D, Chen J, Kumar V, Iwakura Y, Lu T
    J Immunol, 2014;192(9):4153-63.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In vivo
  12. A tissue-specific role for Nlrp3 in tubular epithelial repair after renal ischemia/reperfusion.
    Authors: Bakker P, Butter L, Claessen N, Teske G, Sutterwala F, Florquin S, Leemans J
    Am J Pathol, 2014;184(7):2013-22.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: IP
  13. Regulator of calcineurin 1 suppresses inflammation during respiratory tract infections.
    Authors: Junkins R, MacNeil A, Wu Z, McCormick C, Lin T
    J Immunol, 2013;190(10):5178-86.
    Species: Mouse
    Sample Types: BALF
    Applications: ELISA Capture
  14. NLRP10 is a NOD-like receptor essential to initiate adaptive immunity by dendritic cells.
    Authors: Eisenbarth, Stephani, Williams, Adam, Colegio, Oscar R, Meng, Hailong, Strowig, Till, Rongvaux, Anthony, Henao-Mejia, Jorge, Thaiss, Christop, Joly, Sophie, Gonzalez, David G, Xu, Lan, Zenewicz, Lauren A, Haberman, Ann M, Elinav, Eran, Kleinstein, Steven H, Sutterwala, Fayyaz S, Flavell, Richard
    Nature, 2012;484(7395):510-3.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  15. Response patterns of cytokines/chemokines in two murine strains after irradiation.
    Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
    Cytokine, 2012;58(2):169-77.
    Species: Mouse
    Sample Types: Plasma
    Applications: Luminex Assay Development
  16. The CDK domain of p21 is a suppressor of IL-1beta-mediated inflammation in activated macrophages.
    Authors: Scatizzi JC, Mavers M, Hutcheson J, Young B, Shi B, Pope RM, Ruderman EM, Samways DS, Corbett JA, Egan TM, Perlman H
    Eur. J. Immunol., 2009;39(3):820-5.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: WB
  17. Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin.
    Authors: Wu W, Mehta H, Chakrabarty K, Booth JL, Duggan ES, Patel KB, Ballard JD, Coggeshall KM, Metcalf JP
    J. Immunol., 2009;183(9):5799-806.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  18. Stimulation of Toll-like receptor 3 and 4 induces interleukin-1beta maturation by caspase-8.
    Authors: Maelfait J, Vercammen E, Janssens S, Schotte P, Haegman M, Magez S, Beyaert R
    J. Exp. Med., 2008;205(9):1967-73.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: WB
  19. TNF-alpha and IL-1beta mediate inflammatory hypernociception in mice triggered by B1 but not B2 kinin receptor.
    Authors: Cunha TM, Verri WA, Jr, Fukada SY, Guerrero AT, Santodomingo-Garzon T, Poole S, Parada CA, Ferreira SH, Cunha FQ
    Eur. J. Pharmacol., 2007;573(1):221-9.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Development
  20. Maternal immune activation alters fetal brain development through interleukin-6.
    Authors: Smith SE, Li J, Garbett K, Mirnics K, Patterson PH
    J. Neurosci., 2007;27(40):10695-702.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In vivo
  21. Antiinflammatory effects of natural tetranortriterpenoids isolated from Carapa guianensis Aublet on zymosan-induced arthritis in mice.
    Authors: Penido C, Conte FP, Chagas MS, Rodrigues CA, Pereira JF, Henriques MG
    Inflamm. Res., 2006;55(11):457-64.
    Species: Mouse
    Sample Types: Synovial Fluid
    Applications: ELISA Development
  22. Blockade of experimental atopic dermatitis via topical NF-kappaB decoy oligonucleotide.
    Authors: Dajee M, Muchamuel T, Schryver B, Oo A, Alleman-Sposeto J, De Vry CG, Prasad S, Ruhrmund D, Shyamsundar R, Mutnick D, Mai K, Le T, Parham C, Zhang J, Komuves L, Colby T, Hudak S, McEvoy LM, Ehrhardt RO
    J. Invest. Dermatol., 2006;126(8):1792-803.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Frozen
  23. Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality.
    Authors: Osuchowski MF, Welch K, Siddiqui J, Remick DG
    J. Immunol., 2006;177(3):1967-74.
    Species: Mouse
    Sample Types: Plasma
    Applications: ELISA Development
  24. Sequential ELISA to profile multiple cytokines from small volumes.
    Authors: Osuchowski MF, Siddiqui J, Copeland S, Remick DG
    J. Immunol. Methods, 2005;302(1):172-81.
    Species: Mouse
    Sample Types: Plasma
    Applications: ELISA Development
  25. Selective macrophage suppression during sepsis.
    Authors: Ellaban E, Bolgos G, Remick D
    Cell. Immunol., 2004;231(1):103-11.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development

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Mouse IL‑1 beta /IL‑1F2 Antibody
By Anonymous on 05/17/2018
Application: ELISA Sample Tested: 293T human embryonic kidney cell line Species: Human