Mouse IL-1 beta /IL-1F2 Antibody

Name: Mouse IL-1 beta /IL-1F2 Antibody
Brand: R&D Systems
Rating: 5 (1 reviews)

MAB401 27 Citations 1 Review Datasheet / COA / SDS

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Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutralization by Mouse IL‑1 beta /IL‑1F2 Antibody.

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Citations (27)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IL-1 beta /IL-1F2 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IL-1 beta /IL-1F2 in ELISAs.

Source

Monoclonal Rat IgG₁ Clone # 30311

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse IL-1 beta /IL-1F2
Val118-Ser269
Accession # P10749

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Mouse IL-1 beta /IL-1F2 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Mouse IL‑1 beta /IL‑1F2 Biotinylated Antibody (Catalog # BAF401)

Standard: Recombinant Mouse IL-1 beta/IL-1F2 Protein (Catalog # 401-ML)

Neutralization

Measured by its ability to neutralize IL‑1 beta /IL‑1F2-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND₅₀) is typically 1-5 µg/mL in the presence of 50 pg/mL Recombinant Mouse IL‑1 beta /IL‑1F2 and 1.25 µg/mL concanavalin A.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization

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Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutralization by Mouse IL‑1 beta /IL‑1F2 Antibody. Recombinant Mouse IL‑1 beta /IL‑1F2 (Catalog # 401-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑1 beta /IL‑1F2 (50 pg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB401). The ND₅₀ is typically 1-5 µg/mL in the presence of concanavalin A (1.25 µg/mL).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-1 beta/IL-1F2

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 17% amino acid (aa) identity in mouse. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1-4). The mouse IL-1 beta cDNA encodes a 269 aa precursor. A 117 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5, 6). The 17 kDa mature mouse IL-1 beta shares 90% aa sequence identity with cotton rat and rat and 65-78% identity with canine, equine, feline, human, porcine, and rhesus macaque IL‑1 beta.

References

Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629.
Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S.
Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21.
Gray, P.W. et al. (1986) J. Immunol. 137:3644.
Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.

Long Name

Interleukin 1 beta

Entrez Gene IDs

3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)

Alternate Names

catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse IL-1 beta /IL-1F2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

27 Citations: Showing 1 - 10
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Obesity-associated inflammation promotes angiogenesis and breast cancer via angiopoietin-like 4
Authors: R Kolb, P Kluz, ZW Tan, N Borcherdin, N Bormann, A Vishwakarm, L Balcziak, P Zhu, BS Davies, F Gourronc, LZ Liu, X Ge, BH Jiang, K Gibson-Cor, A Klingelhut, NS Tan, Y Zhu, FS Sutterwala, X Shen, W Zhang
Oncogene, 2018;0(0):.
Species: Mouse
Sample Types: Cell Lysate
Applications: ELISA Capture
New mitochondrial DNA synthesis enables NLRP3 inflammasome activation
Authors: Z Zhong, S Liang, E Sanchez-Lo, F He, S Shalapour, XJ Lin, J Wong, S Ding, E Seki, B Schnabl, AL Hevener, HB Greenberg, T Kisseleva, M Karin
Nature, 2018;0(0):.
Species: Mouse
Sample Types: Serum
Anti-inflammatory effects of a traditional Korean medicine: Ojayeonjonghwan
Authors: SY Nam, KY Kim, MH Kim, JB Jang, SY Rah, HJ Chae, JM Lee, HM Kim, HJ Jeong
Pharm Biol, 2017;55(1):1856-1862.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Capture
Mucin 1 protects against severe Streptococcus pneumoniae infection
Authors: P Dhar, GZ Ng, EM Dunne, P Sutton
Virulence, 2017;0(0):0.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: ELISA Capture
The calcineurin-NFAT axis contributes to host defense during Pseudomonas aeruginosa lung infection
Authors: Z Pang, RD Junkins, AJ MacNeil, C McCormick, Z Cheng, WM Chen, TJ Lin
J. Leukoc. Biol., 2017;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: ELISA Capture
Inhibitory effect ofquercetinon titaniumparticle-inducedendoplasmic reticulum stress (ERS)-related apoptosisandin vivoosteolysis
Authors: L Zhang, Z Tian, W Li, X Wang, Z Man, S Sun
Biosci. Rep., 2017;0(0):.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Capture
Anti-inflammatory Effects of the Natural Compounds Cortex Phellodendri and Humulus japonicus on Pelvic Inflammatory Disease in Mice
Authors: Y Oh, YS Kwon, BD Jung
Int J Med Sci, 2017;14(8):729-734.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: ELISA Capture
Inhibiting the NLRP3 inflammasome with MCC950 promotes non-phlogistic clearance of amyloid-? and cognitive function in APP/PS1 mice
Authors: C Dempsey
Brain Behav. Immun, 2016;0(0):.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Capture
Oryza sativa (Rice) Hull Extract Inhibits Lipopolysaccharide-Induced Inflammatory Response in RAW264.7 Macrophages by Suppressing Extracellular Signal-regulated Kinase, c-Jun N-terminal Kinase, and Nuclear Factor-?B Activation
Authors: Yoonsook Kim
Pharmacogn Mag, 2016;12(48):295-301.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Capture
AMCase is a crucial regulator of type 2 immune responses to inhaled house dust mites.
Authors: Kim L, Morita R, Kobayashi Y, Eisenbarth S, Lee C, Elias J, Eynon E, Flavell R
Proc Natl Acad Sci U S A, 2015;112(22):E2891-9.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Capture

Myeloid cell TRAF3 regulates immune responses and inhibits inflammation and tumor development in mice.
Authors: Lalani A, Moore C, Luo C, Kreider B, Liu Y, Morse H, Xie P
J Immunol, 2015;194(1):334-48.
Species: Mouse
Sample Types: Serum
Applications: ELISA Development

A tissue-specific role for Nlrp3 in tubular epithelial repair after renal ischemia/reperfusion.
Authors: Bakker P, Butter L, Claessen N, Teske G, Sutterwala F, Florquin S, Leemans J
Am J Pathol, 2014;184(7):2013-22.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: IP

Multiple CD11c+ cells collaboratively express IL-1beta to modulate stromal vascular endothelial growth factor and lymph node vascular-stromal growth.
Authors: Benahmed F, Chyou S, Dasoveanu D, Chen J, Kumar V, Iwakura Y, Lu T
J Immunol, 2014;192(9):4153-63.
Species: Mouse
Sample Types: In Vivo
Applications: In vivo

Glimepiride reduces CD14 expression and cytokine secretion from macrophages.
Authors: Ingham V, Williams A, Bate C
J Neuroinflammation, 2014;11(0):115.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Capture

Regulator of calcineurin 1 suppresses inflammation during respiratory tract infections.
Authors: Junkins R, MacNeil A, Wu Z, McCormick C, Lin T
J Immunol, 2013;190(10):5178-86.
Species: Mouse
Sample Types: BALF
Applications: ELISA Capture

Response patterns of cytokines/chemokines in two murine strains after irradiation.
Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
Cytokine, 2012;58(2):169-77.
Species: Mouse
Sample Types: Plasma
Applications: Luminex Assay Development

NLRP10 is a NOD-like receptor essential to initiate adaptive immunity by dendritic cells.
Authors: Eisenbarth, Stephani, Williams, Adam, Colegio, Oscar R, Meng, Hailong, Strowig, Till, Rongvaux, Anthony, Henao-Mejia, Jorge, Thaiss, Christop, Joly, Sophie, Gonzalez, David G, Xu, Lan, Zenewicz, Lauren A, Haberman, Ann M, Elinav, Eran, Kleinstein, Steven H, Sutterwala, Fayyaz S, Flavell, Richard
Nature, 2012;484(7395):510-3.
Species: Mouse
Sample Types: Cell Culture Supernates

Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin.
Authors: Wu W, Mehta H, Chakrabarty K, Booth JL, Duggan ES, Patel KB, Ballard JD, Coggeshall KM, Metcalf JP
J. Immunol., 2009;183(9):5799-806.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Development

The CDK domain of p21 is a suppressor of IL-1beta-mediated inflammation in activated macrophages.
Authors: Scatizzi JC, Mavers M, Hutcheson J, Young B, Shi B, Pope RM, Ruderman EM, Samways DS, Corbett JA, Egan TM, Perlman H
Eur. J. Immunol., 2009;39(3):820-5.
Species: Mouse
Sample Types: Cell Lysates
Applications: WB

Stimulation of Toll-like receptor 3 and 4 induces interleukin-1beta maturation by caspase-8.
Authors: Maelfait J, Vercammen E, Janssens S, Schotte P, Haegman M, Magez S, Beyaert R
J. Exp. Med., 2008;205(9):1967-73.
Species: Mouse
Sample Types: Cell Lysates
Applications: WB

TNF-alpha and IL-1beta mediate inflammatory hypernociception in mice triggered by B1 but not B2 kinin receptor.
Authors: Cunha TM, Verri WA, Jr, Fukada SY, Guerrero AT, Santodomingo-Garzon T, Poole S, Parada CA, Ferreira SH, Cunha FQ
Eur. J. Pharmacol., 2007;573(1):221-9.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: ELISA Development

Maternal immune activation alters fetal brain development through interleukin-6.
Authors: Smith SE, Li J, Garbett K, Mirnics K, Patterson PH
J. Neurosci., 2007;27(40):10695-702.
Species: Mouse
Sample Types: In Vivo
Applications: In vivo

Antiinflammatory effects of natural tetranortriterpenoids isolated from Carapa guianensis Aublet on zymosan-induced arthritis in mice.
Authors: Penido C, Conte FP, Chagas MS, Rodrigues CA, Pereira JF, Henriques MG
Inflamm. Res., 2006;55(11):457-64.
Species: Mouse
Sample Types: Synovial Fluid
Applications: ELISA Development

Blockade of experimental atopic dermatitis via topical NF-kappaB decoy oligonucleotide.
Authors: Dajee M, Muchamuel T, Schryver B, Oo A, Alleman-Sposeto J, De Vry CG, Prasad S, Ruhrmund D, Shyamsundar R, Mutnick D, Mai K, Le T, Parham C, Zhang J, Komuves L, Colby T, Hudak S, McEvoy LM, Ehrhardt RO
J. Invest. Dermatol., 2006;126(8):1792-803.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC Frozen

Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality.
Authors: Osuchowski MF, Welch K, Siddiqui J, Remick DG
J. Immunol., 2006;177(3):1967-74.
Species: Mouse
Sample Types: Plasma
Applications: ELISA Development

Sequential ELISA to profile multiple cytokines from small volumes.
Authors: Osuchowski MF, Siddiqui J, Copeland S, Remick DG
J. Immunol. Methods, 2005;302(1):172-81.
Species: Mouse
Sample Types: Plasma
Applications: ELISA Development

Selective macrophage suppression during sepsis.
Authors: Ellaban E, Bolgos G, Remick D
Cell. Immunol., 2004;231(1):103-11.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Development