Mouse IL-1 beta /IL-1F2 Antibody Summary
Val118-Ser269
Accession # P10749
Applications
Mouse IL-1 beta /IL-1F2 Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example

Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutral-ization by Mouse IL‑1 beta /IL‑1F2 Antibody. Recombinant Mouse IL-1 beta /IL-1F2 (Catalog # 401-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-1 beta /IL-1F2 (50 pg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB401). The ND50 is typically 1-5 µg/mL.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-1 beta/IL-1F2
IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2, IL1B), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 17% amino acid (aa) identity in mouse. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. The 17 kDa molecular weight mature mouse IL-1 beta shares 90% aa sequence identity with cotton rat and rat and 67%-78% with canine, equine, feline, human, porcine, and rhesus macaque IL-1 beta. IL-1 beta functions in a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases.
Product Datasheets
Citations for Mouse IL-1 beta /IL-1F2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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&beta-Hydroxybutyrate inhibits inflammasome activation to attenuate Alzheimer's disease pathology
Authors: DC Shippy, C Wilhelm, PA Viharkumar, TJ Raife, TK Ulland
Journal of Neuroinflammation, 2020;17(1):280.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Capture -
MicroRNA-29b Enhances Osteoclast Survival by Targeting BCL-2-Modifying Factor after Lipopolysaccharide Stimulation
Authors: OJ Sul, M Rajasekara, HJ Park, JH Suh, HS Choi
Oxid Med Cell Longev, 2019;2019(0):6018180.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Berberine ameliorates lipopolysaccharide?induced inflammatory responses in mouse inner medullary collecting duct?3 cells by downregulation of NF?kappaB pathway
Authors: DG Kim, JW Choi, IJ Jo, MJ Kim, HS Lee, SH Hong, HJ Song, GS Bae, SJ Park
Mol Med Rep, 2019;0(0):.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
Inflammasome is activated in the liver of cholestatic patients and aggravates hepatic injury in bile duct ligated mouse
Authors: SY Cai, M Ge, A Mennone, R Hoque, X Ouyang, JL Boyer
Cell Mol Gastroenterol Hepatol, 2019;0(0):.
Species: Human
Sample Types: RNA
Applications: Bioassay -
Early growth response 1 deficiency protects host against Pseudomonas aeruginosa lung infection
Authors: Z Pang, R Raudonis, C McCormick, Z Cheng
Infect. Immun., 2019;0(0):.
Species: Mouse
Sample Types: BALF
Applications: ELISA Capture -
A20 prevents inflammasome-dependent arthritis by inhibiting macrophage necroptosis through its ZnF7 ubiquitin-binding domain
Authors: A Polykratis, A Martens, RO Eren, Y Shirasaki, M Yamagishi, Y Yamaguchi, S Uemura, M Miura, B Holzmann, G Kollias, M Armaka, G van Loo, M Pasparakis
Nat. Cell Biol., 2019;0(0):.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: Bioassay -
New mitochondrial DNA synthesis enables NLRP3 inflammasome activation
Authors: Z Zhong, S Liang, E Sanchez-Lo, F He, S Shalapour, XJ Lin, J Wong, S Ding, E Seki, B Schnabl, AL Hevener, HB Greenberg, T Kisseleva, M Karin
Nature, 2018;0(0):.
Species: Mouse
Sample Types: Serum
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Obesity-associated inflammation promotes angiogenesis and breast cancer via angiopoietin-like 4
Authors: R Kolb, P Kluz, ZW Tan, N Borcherdin, N Bormann, A Vishwakarm, L Balcziak, P Zhu, BS Davies, F Gourronc, LZ Liu, X Ge, BH Jiang, K Gibson-Cor, A Klingelhut, NS Tan, Y Zhu, FS Sutterwala, X Shen, W Zhang
Oncogene, 2018;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: ELISA Development (Capture) -
TNF Family Member LIGHT Acts with IL-1? and TGF-? to Promote Airway Remodeling During Rhinovirus Infection
Authors: AK Mehta, T Doherty, D Broide, M Croft
Allergy, 2018;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
The calcineurin-NFAT axis contributes to host defense during Pseudomonas aeruginosa lung infection
Authors: Z Pang, RD Junkins, AJ MacNeil, C McCormick, Z Cheng, WM Chen, TJ Lin
J. Leukoc. Biol., 2017;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: ELISA Development (Capture) -
Anti-inflammatory Effects of the Natural Compounds Cortex Phellodendri and Humulus japonicus on Pelvic Inflammatory Disease in Mice
Authors: Y Oh, YS Kwon, BD Jung
Int J Med Sci, 2017;14(8):729-734.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: ELISA Development (Capture) -
Inhibitory effect ofquercetinon titaniumparticle-inducedendoplasmic reticulum stress (ERS)-related apoptosisandin vivoosteolysis
Authors: L Zhang, Z Tian, W Li, X Wang, Z Man, S Sun
Biosci. Rep., 2017;0(0):.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Development (Capture) -
Mucin 1 protects against severe Streptococcus pneumoniae infection
Authors: P Dhar, GZ Ng, EM Dunne, P Sutton
Virulence, 2017;0(0):0.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: ELISA Development (Capture) -
Anti-inflammatory effects of a traditional Korean medicine: Ojayeonjonghwan
Authors: SY Nam, KY Kim, MH Kim, JB Jang, SY Rah, HJ Chae, JM Lee, HM Kim, HJ Jeong
Pharm Biol, 2017;55(1):1856-1862.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Development (Capture) -
Inhibiting the NLRP3 inflammasome with MCC950 promotes non-phlogistic clearance of amyloid-? and cognitive function in APP/PS1 mice
Authors: C Dempsey
Brain Behav. Immun, 2016;0(0):.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Development (Capture) -
Oryza sativa (Rice) Hull Extract Inhibits Lipopolysaccharide-Induced Inflammatory Response in RAW264.7 Macrophages by Suppressing Extracellular Signal-regulated Kinase, c-Jun N-terminal Kinase, and Nuclear Factor-?B Activation
Authors: Yoonsook Kim
Pharmacogn Mag, 2016;12(48):295-301.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Development (Capture) -
Myeloid cell TRAF3 regulates immune responses and inhibits inflammation and tumor development in mice.
Authors: Lalani A, Moore C, Luo C, Kreider B, Liu Y, Morse H, Xie P
J Immunol, 2015;194(1):334-48.
Species: Mouse
Sample Types: Serum
Applications: ELISA Development -
AMCase is a crucial regulator of type 2 immune responses to inhaled house dust mites.
Authors: Kim L, Morita R, Kobayashi Y, Eisenbarth S, Lee C, Elias J, Eynon E, Flavell R
Proc Natl Acad Sci U S A, 2015;112(22):E2891-9.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Development (Capture) -
Multiple CD11c+ cells collaboratively express IL-1beta to modulate stromal vascular endothelial growth factor and lymph node vascular-stromal growth.
Authors: Benahmed F, Chyou S, Dasoveanu D, Chen J, Kumar V, Iwakura Y, Lu T
J Immunol, 2014;192(9):4153-63.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Glimepiride reduces CD14 expression and cytokine secretion from macrophages.
Authors: Ingham V, Williams A, Bate C
J Neuroinflammation, 2014;11(0):115.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Development (Capture) -
A tissue-specific role for Nlrp3 in tubular epithelial repair after renal ischemia/reperfusion.
Authors: Bakker P, Butter L, Claessen N, Teske G, Sutterwala F, Florquin S, Leemans J
Am J Pathol, 2014;184(7):2013-22.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Immunoprecipitation -
Regulator of calcineurin 1 suppresses inflammation during respiratory tract infections.
Authors: Junkins R, MacNeil A, Wu Z, McCormick C, Lin T
J Immunol, 2013;190(10):5178-86.
Species: Mouse
Sample Types: BALF
Applications: ELISA Development (Capture) -
Response patterns of cytokines/chemokines in two murine strains after irradiation.
Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
Cytokine, 2012;58(2):169-77.
Species: Mouse
Sample Types: Plasma
Applications: Luminex Development -
NLRP10 is a NOD-like receptor essential to initiate adaptive immunity by dendritic cells.
Authors: Eisenbarth, Stephani, Williams, Adam, Colegio, Oscar R, Meng, Hailong, Strowig, Till, Rongvaux, Anthony, Henao-Mejia, Jorge, Thaiss, Christop, Joly, Sophie, Gonzalez, David G, Xu, Lan, Zenewicz, Lauren A, Haberman, Ann M, Elinav, Eran, Kleinstein, Steven H, Sutterwala, Fayyaz S, Flavell, Richard
Nature, 2012;484(7395):510-3.
Species: Mouse
Sample Types: Cell Culture Supernates
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Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin.
Authors: Wu W, Mehta H, Chakrabarty K, Booth JL, Duggan ES, Patel KB, Ballard JD, Coggeshall KM, Metcalf JP
J. Immunol., 2009;183(9):5799-806.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
The CDK domain of p21 is a suppressor of IL-1beta-mediated inflammation in activated macrophages.
Authors: Scatizzi JC, Mavers M, Hutcheson J, Young B, Shi B, Pope RM, Ruderman EM, Samways DS, Corbett JA, Egan TM, Perlman H
Eur. J. Immunol., 2009;39(3):820-5.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Stimulation of Toll-like receptor 3 and 4 induces interleukin-1beta maturation by caspase-8.
Authors: Maelfait J, Vercammen E, Janssens S, Schotte P, Haegman M, Magez S, Beyaert R
J. Exp. Med., 2008;205(9):1967-73.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Maternal immune activation alters fetal brain development through interleukin-6.
Authors: Smith SE, Li J, Garbett K, Mirnics K, Patterson PH
J. Neurosci., 2007;27(40):10695-702.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
TNF-alpha and IL-1beta mediate inflammatory hypernociception in mice triggered by B1 but not B2 kinin receptor.
Authors: Cunha TM, Verri WA, Jr, Fukada SY, Guerrero AT, Santodomingo-Garzon T, Poole S, Parada CA, Ferreira SH, Cunha FQ
Eur. J. Pharmacol., 2007;573(1):221-9.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: ELISA Development -
Antiinflammatory effects of natural tetranortriterpenoids isolated from Carapa guianensis Aublet on zymosan-induced arthritis in mice.
Authors: Penido C, Conte FP, Chagas MS, Rodrigues CA, Pereira JF, Henriques MG
Inflamm. Res., 2006;55(11):457-64.
Species: Mouse
Sample Types: Synovial Fluid
Applications: ELISA Development -
Blockade of experimental atopic dermatitis via topical NF-kappaB decoy oligonucleotide.
Authors: Dajee M, Muchamuel T, Schryver B, Oo A, Alleman-Sposeto J, De Vry CG, Prasad S, Ruhrmund D, Shyamsundar R, Mutnick D, Mai K, Le T, Parham C, Zhang J, Komuves L, Colby T, Hudak S, McEvoy LM, Ehrhardt RO
J. Invest. Dermatol., 2006;126(8):1792-803.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality.
Authors: Osuchowski MF, Welch K, Siddiqui J, Remick DG
J. Immunol., 2006;177(3):1967-74.
Species: Mouse
Sample Types: Plasma
Applications: ELISA Development -
Sequential ELISA to profile multiple cytokines from small volumes.
Authors: Osuchowski MF, Siddiqui J, Copeland S, Remick DG
J. Immunol. Methods, 2005;302(1):172-81.
Species: Mouse
Sample Types: Plasma
Applications: ELISA Development -
Selective macrophage suppression during sepsis.
Authors: Ellaban E, Bolgos G, Remick D
Cell. Immunol., 2004;231(1):103-11.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Development
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