Mouse IL-20 Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse IL-20 in direct ELISAs and the Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human IL-20 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant mouse IL‑20
    Leu25-Leu176
    Accession # Q9JKV9
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse IL‑20 (Catalog # 1204-ML)
  • Neutralization
    Measured by its ability to neutralize IL‑20-induced proliferation in the BaF3 mouse pro‑B cell line co-transfected with human IL‑20 R alpha and IL‑20 R beta. The Neutralization Dose (ND50) is typically 0.2-0.6 µg/mL in the presence of 10 ng/mL Recombinant Mouse IL‑20.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Proliferation Induced by IL‑20 and Neutralization by Mouse IL‑20 Antibody. Recombinant Mouse IL‑20 (Catalog # 1204-ML) stimulates proliferation in the BaF3 mouse pro‑B cell line co-transfected with human IL‑20 R alpha and IL‑20 R beta in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑20 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL‑20 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1204). The ND50 is typically 0.2-0.6 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-20

Mouse Interleukin 20 (IL-20) was identified by searching sequence databases for translated sequences with a signal sequence and amphipathic helices found in helical cytokines. Based on the human molecule, mouse IL-20 was discovered in a skin library. Mouse IL-20 is synthesized as a 176 amino acid (aa) precursor that contains a 24 aa signal sequence and a 152 aa mature segment. There are no N-linked glycosylation sites and it is doubtful that the native molecule is glycosylated. Although IL-20 is a distant member of the IL-10 family, it functions as a monomer. IL-20 shares less than 40% aa sequence identity with other IL-10 family members. Mouse and human IL-20 are 77% aa identical in the mature segment. IL-20 production has been found in skin and trachea. In particular, activated keratinocytes and, possibly, monocytes are reported to express IL-20. There are two heterodimeric receptor complexes for IL-20. The first complex is composed of IL-20 R alpha and IL‑20 R beta. The second complex is composed of IL-22 R and IL‑20 R beta. Whereas the IL‑22 R/IL‑20 R beta  complex is shared with IL-24/mda-7, the IL‑20 R alpha /IL‑20 R beta complex is shared with both IL-19 and IL-24. Little is known about the function of IL-20. It is reported to induce the proliferation of multipotential hematopoietic progenitor cells, direct the differentiation and expansion of keratinocytes, and promote the release of proinflammatory mediators in keratinocytes and other IL-20 receptor expressing cells (1‑6).

  • References:
    1. Blumberg, H. et al. (2001) Cell 104:9.
    2. Liu, L. et al. (2003) Blood 102:3206.
    3. Rich, B.E. and T.S. Kupper (2001) Curr. Biol. 11:R531.
    4. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
    5. Dumoutier, L. (2001) J. Immunol. 167:3545. 
    6. Romer, J. (2003) J. Invest. Dermatol. 121:1306.
  • Long Name:
    Interleukin 20
  • Entrez Gene IDs:
    50604 (Human); 58181 (Mouse)
  • Alternate Names:
    Cytokine Zcyto10; IL10D; IL20; IL-20; IL-20ZCYTO10four alpha helix cytokine; interleukin 20; interleukin-20; MGC96907
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