Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all IL-3R alpha Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse IL‑3 R alpha /CD123
Ser17-Lys331
Accession # P26952

Specificity

Detects mouse IL‑3 R alpha /CD123 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant human IL-3 R alpha and recombinant mouse IL-3 R beta is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse IL-3R alpha/CD123 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse IL‑3 R alpha /CD123 Fc Chimera (Catalog # 983-MR)

Reviewed Applications

Read 1 review rated 3 using AF983 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-3R alpha

Interleukin 3 (IL-3) is a pleiotropic cytokine produced primarily by activated T cells or mast cells. IL-3 stimulates the proliferation and differentiation of hemopoietic cells including the pluripotent hematopoietic stem cells as well as various lineage-committed cells. The biological effects of IL-3 on the various cell types are mediated by the binding of IL-3 to specific cell surface receptor complexes. The functional high-affinity IL-3 receptor is a heterodimer consisting of a ligand binding alpha  subunit and the beta  subunit. The alpha  subunit alone binds IL-3 with low affinity. The beta  subunit is required for the high-affinity binding of IL-3 to the heterodimeric receptor complex. The beta  subunit has also been found to be a component of the high-affinity receptor complex for IL-5 and GM-CSF and is also referred to as the beta common ( beta c) chain. In the mouse, there are two IL-3 R beta proteins. The first identified mouse IL-3 R beta was also called AIC2A and binds IL-3 with low affinity (1). The second mIL-3 R beta was referred to as AIC2B (2). AIC2B doesn’t bind IL-3 and is the homolog of the human IL-3 R beta. AIC2A was found to be the result of a gene duplication event. The mouse IL-3 R alpha, also called SUT-1, will form complexes with either mouse IL-3 R beta protein (3). Both the alpha and the beta  subunits are members of the cytokine receptor superfamily.

References

  1. Itoh, N. et al. (1990) Science 247:324.
  2. Gorman, D.M. et al. (1990) Proc. Natl. Acad. Sci. USA 87:5459.
  3. Hara, T. and A. Miyajima (1992) EMBO J. 11:1875.
  4. Schrader, J.W. (2001) Cytokine Reference, Oppenheim, J.J. and M. Feldmann, eds, Academic Press, New York, p. 1899.

Long Name

Interleukin 3 Receptor alpha

Alternate Names

CD123, IL-3 R alpha, IL-3Ra, IL3R alpha, IL3RA

Entrez Gene IDs

3563 (Human); 16188 (Mouse); 102138639 (Cynomolgus Monkey)

Gene Symbol

IL3RA

UniProt

P26952

Additional IL-3R alpha Products

Product Documents for Mouse IL-3R alpha/CD123 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse IL-3R alpha/CD123 Antibody

For research use only

Citations for Mouse IL-3R alpha/CD123 Antibody

Customer Reviews for Mouse IL-3R alpha/CD123 Antibody (1)

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  • Mouse IL-3 R alpha /CD123 Antibody
    Name: Anonymous
    Application: Simple Western
    Sample Tested: Mouse Spleen
    Species: Mouse
    Verified Customer | Posted 06/04/2019
    Homogenized mouse spleen probed at the indicated concentrations, using a 1:25 dil of the antibody.
    Mouse IL-3R alpha/CD123 Antibody AF983

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