Mouse IL-3 R beta Antibody

    
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  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse IL‑3 R beta in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human Common  beta  chain is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse IL-3 R beta
    His23-Trp440
    Accession # P26954
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse IL‑3 R beta Fc Chimera (Catalog # 549-R3)
  • Flow Cytometry
    0.25 µg/106 cells
    DA3 mouse myeloma cell line
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Neutralization
    Measured by its ability to neutralize IL‑3-induced proliferation in the NFS‑60 mouse myelogenous leukemia lymphoblast cell line. Holmes, K. L. et al. (1985) Proc. Natl. Acad. Sci. USA 82:6687. The Neutralization Dose (ND50) is typically 5-15 µg/mL in the presence of 0.5 ng/mL Recombinant Mouse IL‑3.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Cell Proliferation Induced by IL‑3 and Neutralization by Mouse IL‑3 R beta Antibody.

Recombinant Mouse IL‑3 (Catalog # 403-ML) stimulates proliferation in the NFS‑60 mouse myelogenous leukemia lymphoblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑3 (0.5 ng/mL) is neutralized (green line) by increasing concen­trations of Goat Anti-Mouse
IL‑3 R beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF549). The ND50 is typically
5‑15 µg/mL.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-3 R beta

Interleukin 3 (IL-3) is a pleiotropic cytokine produced primarily by activated T cells or mast cells. IL-3 stimulates the proliferation and differentiation of hemopoietic cells including the pluripotent hematopoietic stem cells as well as various lineage-committed cells. The biological effects of IL-3 on the various cell types are mediated by the binding of IL-3 to specific cell surface receptor complexes. The functional high-affinity IL-3 receptor is a heterodimer consisting of a ligand binding alpha subunit and the beta subunit. The alpha subunit alone binds IL-3 with low affinity. The beta subunit is required for the high-affinity binding of IL-3 to the heterodimeric receptor complex. The beta subunit has also been found to be a component of the high-affinity receptor complex for IL-5 and GM-CSF and is also referred to as the beta common ( beta c) chain. In the mouse, there are two IL-3 R beta proteins. The first identified mouse IL-3 R beta was also called AIC2A and binds IL-3 with low affinity (1). The second mIL-3 R beta was referred to as AIC2B (2). AIC2B doesn’t bind IL-3 and is the homolog of the human IL-3 R beta. AIC2A was found to be the result of a gene duplication event. Both the alpha  and the beta  subunits are members of the cytokine receptor superfamily (3).

  • References:
    1. Itoh, N. et al. (1990) Science 247:324.
    2. Gorman, D.M. et al. (1990) Proc. Natl. Acad. Sci. USA 87:5459.
    3. Schrader, J.W. in Cytokine Reference, (2001) J.J. Oppenheim and M. Feldmann, eds. Academic Press, New York, p. 1899.
  • Long Name:
    Interleukin 3 Receptor beta
  • Entrez Gene IDs:
    12984 (Mouse)
  • Alternate Names:
    AIC2A; BetaIl3; Bil3; Csfgmrb; IL-3 R beta; IL3R beta; IL3R; IL3RB; IL-3Rb; Il3rb2
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Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 203  
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Reconstitution Buffers
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Reconstitution Buffer 1 (PBS)

RB01
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Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG HRP-conjugated Antibody

WB , Simple Western HAF109 20  
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Goat IgG HRP-conjugated Antibody

WB HAF017 16  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow , IHC , ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow , IHC , ICC NL003 6
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 7  
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow , IHC , ICC NL002
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB , ELISA R-401-C-ABS
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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