Mouse IL-3R beta Antibody

R&D Systems | Catalog # AF549

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Neutralization, Flow Cytometry, CyTOF-ready

Cited:

Neutralization, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all IL-3R beta Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse IL-3 R beta
His23-Trp440
Accession # P26954

Specificity

Detects mouse IL‑3 R beta in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human Common  beta  chain is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse IL-3R beta Antibody

Cell Proliferation Induced by IL‑3 and Neutralization by Mouse IL‑3 R beta Antibody.

Cell Proliferation Induced by IL‑3 and Neutralization by Mouse IL‑3 R beta Antibody.

Recombinant Mouse IL-3 (403-ML) stimulates proliferation in the NFS-60 mouse myelogenous leukemia lymphoblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-3 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-3 R beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF549). The ND50 is typically 5-15 µg/mL.

Detection of IL-3R beta in mouse bone marrow by Flow Cytometry

Mouse bone marrow cells were stained with Rat Anti-Mouse CD11b/Integrin alpha M PE‑conjugated Monoclonal Antibody (Catalog # FAB1124P) and either (A) Goat Anti-Mouse IL-3R beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF549) or (B) isotype control antibody (Catalog # AB-108-C) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse IL-3R beta Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Mouse bone marrow

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse IL‑3 R beta Fc Chimera (Catalog # 549-R3)

Neutralization

Measured by its ability to neutralize IL‑3-induced proliferation in the NFS‑60 mouse myelogenous leukemia lymphoblast cell line. Holmes, K. L. et al. (1985) Proc. Natl. Acad. Sci. USA 82:6687. The Neutralization Dose (ND50) is typically 5-15 µg/mL in the presence of 0.5 ng/mL Recombinant Mouse IL‑3.

Reviewed Applications

Read 1 review rated 1 using AF549 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

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  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-3R beta

Interleukin 3 (IL-3) is a pleiotropic cytokine produced primarily by activated T cells or mast cells. IL-3 stimulates the proliferation and differentiation of hemopoietic cells including the pluripotent hematopoietic stem cells as well as various lineage-committed cells. The biological effects of IL-3 on the various cell types are mediated by the binding of IL-3 to specific cell surface receptor complexes. The functional high-affinity IL-3 receptor is a heterodimer consisting of a ligand binding alpha subunit and the beta subunit. The alpha subunit alone binds IL-3 with low affinity. The beta subunit is required for the high-affinity binding of IL-3 to the heterodimeric receptor complex. The beta subunit has also been found to be a component of the high-affinity receptor complex for IL-5 and GM-CSF and is also referred to as the beta common ( beta c) chain. In the mouse, there are two IL-3 R beta proteins. The first identified mouse IL-3 R beta was also called AIC2A and binds IL-3 with low affinity (1). The second mIL-3 R beta was referred to as AIC2B (2). AIC2B doesn’t bind IL-3 and is the homolog of the human IL-3 R beta. AIC2A was found to be the result of a gene duplication event. Both the alpha  and the beta  subunits are members of the cytokine receptor superfamily (3).

References

  1. Itoh, N. et al. (1990) Science 247:324.
  2. Gorman, D.M. et al. (1990) Proc. Natl. Acad. Sci. USA 87:5459.
  3. Schrader, J.W. in Cytokine Reference, (2001) J.J. Oppenheim and M. Feldmann, eds. Academic Press, New York, p. 1899.

Long Name

Interleukin 3 Receptor beta

Alternate Names

IL-3R beta, IL-3Rb, IL3R beta

Entrez Gene IDs

12984 (Mouse)

Gene Symbol

CSF2RB2

UniProt

P26954

Additional IL-3R beta Products

Product Documents for Mouse IL-3R beta Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse IL-3R beta Antibody

For research use only

Citations for Mouse IL-3R beta Antibody

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  • Mouse IL-3 R beta Antibody
    Name: Anonymous
    Application: Simple Western
    Sample Tested: Mouse Spleen
    Species: Mouse
    Verified Customer | Posted 06/04/2019
    Homogenized mouse spleen probed at the indicated concentrations, using a 1:25 dil of the antibody.
    Mouse IL-3R beta Antibody AF549

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