Mouse IL-31 Antibody

Catalog # Availability Size / Price Qty
AF3028
AF3028-SP
Product Details
Citations (1)
FAQs
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Mouse IL-31 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-31 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human IL-31 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse IL-31
Thr24-Cys163
Accession # Q6EAL8
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse IL‑31

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-31

IL-31 is a 25 kDa, unique member of the short chain group of alpha -helical cytokines. It is produced by activated T cells, particularly the Th2 CD4+ phenotype. Mouse IL‑31 cDNA encodes a 163 amino acid (aa) precursor that contains a 22 aa signal sequence and a 141 aa mature region. IL-31 signals through a receptor complex composed of IL-31 receptor A and Oncostatin M receptor. Mature mouse IL‑31 shares 25% aa sequence identity with mature human IL-31 and 44% aa sequence identity with a rat IL-31-like protein.

Long Name
Interleukin 31
Entrez Gene IDs
386653 (Human); 76399 (Mouse)
Alternate Names
IL31; IL-31; IL-31interleukin-31; interleukin 31

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Citation for Mouse IL-31 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Diverse functions of reactive cysteines facilitate unique biosynthetic processes of aggregate-prone interleukin-31.
    Authors: Shen M, Siu S, Byrd S, Edelmann KH, Patel N, Ketchem RR, Mehlin C, Arnett HA, Hasegawa H
    Exp. Cell Res., 2010-12-21;317(7):976-93.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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