Mouse Klotho Antibody

Catalog # Availability Size / Price Qty
Mouse Klotho ELISA Standard Curve.
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Product Details
Citations (2)
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Reviews (1)

Mouse Klotho Antibody Summary

Species Reactivity
Detects mouse Klotho (aa 23-550 and aa 35-982) in direct ELISAs and Western blots. Does not cross-react with recombinant human Klotho or recombinant mouse Klotho B.
Monoclonal Rat IgG2A Clone # 236214
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant mouse Klotho
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration

This antibody functions as an ELISA capture antibody when paired with Goat Anti-Mouse Klotho Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1819).

This product is intended for assay development on various assay platforms requiring antibody pairs.

8-25 µg/mL
Perfusion fixed frozen sections of mouse kindney and intestine.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Mouse Klotho ELISA Standard Curve. Recombinant Mouse Klotho protein was serially diluted 2-fold and captured by Rat Anti-Mouse Klotho Monoclonal Antibody (Catalog # MAB1819) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Klotho Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1819) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Klotho

Klotho, also called Klotho-alpha, is the founding member of the Klotho family within the glycosidase-1 superfamily (1, 2). Klotho is expressed in areas concerned with calcium regulation, predominantly in the kidney distal convoluted tubules, but also in the brain choroid plexus (which produces cerebrospinal fluid) and the parathyroid (1). The 1014 amino acid (aa) type I transmembrane protein contains a 34 aa signal sequence, a 948 aa extracellular domain (ECD) containing two extracellular glycosidase-like domains, a 21 aa transmembrane domain and an 11 aa intracellular domain. Within the ECD, mouse Klotho shares 95%, 87%, and 87% aa identity with rat, human, and equine Klotho, respectively. Although a truncated 554 aa isoform predicts a soluble 70 kDa form, the soluble form found in plasma and cerebrospinal fluid is a 130 kDa form produced by proteolytic cleavage of the glycosylated 135 kDa full-length Klotho (3, 4). A prominent intracellular 120 kDa form of Klotho is localized to endoplasmic reticulum and Golgi membranes (4). Klotho is named for the Greek goddess who spins the thread of life. The phenotype of Klotho-deficient mice resembles premature aging, including arteriosclerosis, osteoporosis, skin atrophy, infertility, emphysema, and premature death (2). Conversely, excess Klotho extends lifespan (5). Klotho acts as a cofactor for interaction of FGF-23 with FGF R1 (6). This interaction negatively regulates 1 alpha -hydroxylase, the rate-limiting enzyme in the synthesis of 1,25(OH)2D3 (vitamin D) (7). Klotho-deficient mice show severe hyperphosphatemia and ectopic calcification of soft tissues due to excess vitamin D (2, 7). Both Klotho and Klotho-beta are co-factors for FGF-19 binding (8). Klotho also shows glucuronidase activity which activates the renal ion channel TRPV5 to reabsorb urinary calcium (9). Klotho has been reported to downregulate insulin or IGF-I signaling in adipocytes, to bind and antagonize Wnt molecules, and to facilitate release of parathyroid hormone (10-12).

  1. Nabeshima, Y. (2006) Sci. Aging Knowl. Environ. 8:pe11. 
  2. Kuro-o, M. et al. (1997) Nature 390:45. 
  3. Shiraki-Iida, T. et al. (1998) FEBS Lett. 424:6. 
  4. Imura, A. et al. (2004) FEBS Lett. 565:143. 
  5. Kurosu, H. et al. (2005) Science 309:1829. 
  6. Kurosu, H. et al. (2006) J. Biol. Chem. 281:6120. 
  7. Tsujikawa, H. et al. (2003) Mol. Endocrinol. 17:2393.
  8. Wu, X. et al. (2007) J. Biol. Chem. 282:29069.
  9. Chang, Q. et al. (2005) Science 310:490.
  10. Yamamoto, M. et al. (2005) J. Biol. Chem. 280:38029.
  11. Liu, H. et al. (2007) Science 317:803.
  12. Imura, A. et al. (2007) Science 316:1615.
Entrez Gene IDs
9365 (Human); 16591 (Mouse)
Alternate Names
EC 3.2.1; EC; KL; Klotho

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Citations for Mouse Klotho Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Age-related matrix stiffening epigenetically regulates alpha-Klotho expression and compromises chondrocyte integrity
    Authors: H Iijima, G Gilmer, K Wang, AC Bean, Y He, H Lin, WY Tang, D Lamont, C Tai, A Ito, JJ Jones, C Evans, F Ambrosio
    Nature Communications, 2023-01-10;14(1):18.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. The cooperation of FGF receptor and Klotho is involved in excretory canal development and regulation of metabolic homeostasis in Caenorhabditis elegans.
    Authors: Polanska UM, Edwards E, Fernig DG
    J. Biol. Chem., 2010-12-21;286(7):5657-66.
    Species: C. elegans
    Sample Types: Tissue Homogenates
    Applications: Immunoprecipitation, Western Blot


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Mouse Klotho Antibody
By Anonymous on 03/18/2022
Application: IP Sample Tested: C. elegans proteins Species: elegans