Mouse Klotho Antibody AF1819: R&D Systems

Mouse Klotho Antibody

(7 citations)
(2 Reviews)
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Klotho in direct ELISAs and Western blots. In these formats, less than 5% cross-reactivity with recombinant mouse beta -Klotho is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Klotho
    Arg31-His550
    Accession # BAA25307
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse Klotho (Catalog # 1819-KL)
  • Immunohistochemistry
    2-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
Klotho in Mouse Kidney. Klotho was detected in perfusion fixed frozen sections of mouse kidney using 1.7 µg/mL Mouse Klotho Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1819) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Klotho

Klotho, also called Klotho-alpha, is the founding member of the Klotho family within the glycosidase-1 superfamily. Klotho is expressed in areas concerned with calcium regulation, predominantly in the kidney distal convoluted tubules, but also in the brain choroid plexus (which produces cerebrospinal fluid) and the parathyroid. The 1014 amino acid (aa) type I transmembrane protein contains a 34 aa signal sequence, a 948 aa extracellular domain (ECD) containing two extracellular glycosidase-like domains, a 21 aa transmembrane domain and an 11 aa intracellular domain. Within the ECD, mouse Klotho shares 95%, 87%, and 87% aa identity with rat, human, and equine Klotho, respectively. Although a truncated 554 aa isoform predicts a soluble 70 kDa form, the soluble form found in plasma and cerebrospinal fluid is a 130 kDa form produced by proteolytic cleavage of the glycosylated 135 kDa full-length Klotho. A prominent intracellular 120 kDa form of Klotho is localized to endoplasmic reticulum and Golgi membranes. Klotho is named for the Greek goddess who spins the thread of life. The phenotype of Klotho-deficient mice resembles premature aging, including arteriosclerosis, osteoporosis, skin atrophy, infertility, emphysema, and premature death. Conversely, excess Klotho extends lifespan. Klotho acts as a cofactor for interaction of FGF-23 with FGF R1. This interaction negatively regulates 1 alpha -hydroxylase, the rate-limiting enzyme in the synthesis of 1,25(OH)2D3 (vitamin D). Klotho-deficient mice show severe hyperphosphatemia and ectopic calcification of soft tissues due to excess vitamin D. Both Klotho and beta ‑Klotho are co-factors for FGF-19 binding. Klotho also shows glucuronidase activity which activates the renal ion channel TRPV5 to reabsorb urinary calcium. Klotho has been reported to downregulate insulin or IGF-I signaling in adipocytes, to bind and antagonize Wnt molecules, and to facilitate release of parathyroid hormone.

  • Entrez Gene IDs:
    9365 (Human); 16591 (Mouse)
  • Alternate Names:
    EC 3.2.1; EC 3.2.1.31; KL; Klotho
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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Species
Applications
Sample Type
  1. Circulating alphaKlotho influences phosphate handling by controlling FGF23 production.
    Authors: Smith R
    J. Clin. Invest., 2102;122(12):4710-5.
    Species: Mouse
    Sample Type: Plasma
    Application: ELISA Development
  2. Persistent fibroblast growth factor 23 signalling in the parathyroid glands for secondary hyperparathyroidism in mice with chronic kidney disease
    Authors: K Kawakami, A Takeshita, K Furushima, M Miyajima, I Hatamura, M Kuro-O, Y Furuta, K Sakaguchi
    Sci Rep, 2017;7(0):40534.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  3. Antiaging Gene Klotho Attenuates Pancreatic beta-Cell Apoptosis in Type 1 Diabetes.
    Authors: Lin Y, Sun Z
    Diabetes, 2015;64(12):4298-311.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  4. Expression of klotho mRNA and protein in rat brain parenchyma from early postnatal development into adulthood.
    Authors: Clinton S, Glover M, Maltare A, Laszczyk A, Mehi S, Simmons R, King G
    Brain Res, 2013;1527(0):1-14.
    Species: Rat
    Sample Type: Whole Cells
    Application: ICC Fluorescence
  5. Loss of Klotho contributes to kidney injury by derepression of Wnt/beta-catenin signaling.
    Authors: Zhou L, Li Y, Zhou D, Tan R, Liu Y
    J Am Soc Nephrol, 2013;24(5):771-85.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: IP
  6. FGF23 suppresses chondrocyte proliferation in the presence of soluble alpha-Klotho both in vitro and in vivo.
    Authors: Kawai M, Kinoshita S, Kimoto A, Hasegawa Y, Miyagawa K, Yamazaki M, Ohata Y, Ozono K, Michigami T
    J Biol Chem, 2013;288(4):2414-27.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  7. Vitamin D receptor agonists increase klotho and osteopontin while decreasing aortic calcification in mice with chronic kidney disease fed a high phosphate diet.
    Authors: Lau, Wei Ling, Leaf, Elizabet, Hu, Ming Cha, Takeno, Marc M, Kuro-o, Makoto, Moe, Orson W, Giachelli, Cecilia
    Kidney Int, 2012;82(12):1261-70.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Not specified
Cell and Tissue Staining Kits
Description Application Cat# Citations Images  

Anti-Goat HRP-DAB Cell & Tissue Staining Kit

CTS008 13
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Immunohistochemistry Reagents
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Antigen Retrieval Reagent-Universal

CTS015 10
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Antigen Retrieval Reagent-Basic

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Antigen Retrieval Reagent Sampler (50 mL each)

CTS016
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Antigen Retrieval Reagent-Acidic

CTS014
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NL996
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VCTS023
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VCTS022
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VisUCyte Antigen Retrieval Reagent-Basic

VCTS021
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Isotype Controls
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Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
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Goat IgG HRP-conjugated Antibody

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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

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Goat IgG (H+L) APC-conjugated Antibody

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Goat IgG Horseradish Peroxidase-conjugated Antibody

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Donkey Anti-Goat IgG Antibody

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Donkey Anti-Goat IgG Biotinylated Antibody

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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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Average Rating: 4 (Based on 2 reviews)

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We have 1 review tested in 1 species: Mouse.
We have 2 review tested in 1 application: Western Blot.

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Images Ratings Applications Species Reviewed By Date Details
Western Blot Mouse Klotho Antibody AF1819
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Excellent
 WB Mouse Anonymous 12/02/2016
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Western Blot Mouse Klotho Antibody AF1819
Western Blot: Mouse Klotho Antibody [AF1819]

Summary

ApplicationWestern Blot
Sample TestedKidney tissue
SpeciesMouse
Western Blot Mouse Klotho Antibody AF1819
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Average
 WB Anonymous 06/10/2016
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Western Blot Mouse Klotho Antibody AF1819
Western Blot: Mouse Klotho Antibody [AF1819]

Summary

ApplicationWestern Blot
Sample TestedAdult kidney

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