Mouse Klotho Antibody

Catalog # Availability Size / Price Qty
AF1819
AF1819-SP
Detection of Mouse Klotho by Western Blot.
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Product Details
Citations (17)
FAQs
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Reviews (3)

Mouse Klotho Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Klotho in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Klotho
Arg31-His550
Accession # BAA25307
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below
ELISA

This antibody functions as an ELISA detection antibody when paired with Rat Anti-Mouse Klotho Monoclonal Antibody (Catalog # MAB1819).

This product is intended for assay development on various assay platforms requiring antibody pairs.

 
Immunohistochemistry
2-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse Klotho antibody by Western Blot. View Larger

Detection of Mouse Klotho by Western Blot. Western blot shows lysates of mouse kidney tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse Klotho Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1819) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Klotho at approximately 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Klotho antibody in Mouse Kidney by Immunohistochemistry (IHC-Fr). View Larger

Klotho in Mouse Kidney. Klotho was detected in perfusion fixed frozen sections of mouse kidney using 1.7 µg/mL Goat Anti-Mouse Klotho Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1819) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Mouse Klotho ELISA Standard Curve. Recombinant Mouse Klotho protein was serially diluted 2-fold and captured by Rat Anti-Mouse Klotho Monoclonal Antibody (Catalog # MAB1819) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Klotho Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1819) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Western Blot Detection of Human Klotho by Western Blot View Larger

Detection of Human Klotho by Western Blot Ectopic expression of Klotho protects renal mitochondrial homeostasis and inhibits tubular senescence in an accelerated aging mouse model. (a) Renal expression of PGC‐1 alpha mRNA in different groups was assessed by real‐time PCR. *p < .05 versus control mice; †p < .05 versus d‐gal‐treated mice (n = 5–6). (b) Representative micrographs show renal expression of PGC‐1 alpha. Paraffin sections were immunostained for PGC‐1 alpha. Arrows indicate positive staining. Scale bar, 50 µm. (c–h) Representative (c) Western blots and graphical representations of (d) phospho‐PGC‐1 alpha, (e) TFAM, (f) COX1, (g) Cytb, and (h) TOMM20 protein expression levels in different groups are presented. *p < .05 versus control mice; †p < .05 versus d‐gal‐treated mice (n = 5–6). (i) Quantification of renal mitochondrial mass is shown. Mitochondrial mass was determined by the fluorescence intensity of MitoTracker deep red staining normalized to DAPI. *p < .05 versus control mice; †p < .05 versus d‐gal‐treated mice (n = 5–6). (j) Representative micrographs show mitochondrial ROS production and ultrastructure. They were assessed by mitoSOX staining and electron microscopy analyses, respectively. The administration of d‐gal induced mitochondrial ROS production, mitochondrial swelling, and cristae disorganization in renal tubular cells. Arrows indicate positive staining. Ectopic expression of Klotho inhibited mitochondrial ROS production and preserved normal structure of mitochondria. Scale bar in mitoSOX staining, 50 μm. Scale bar in TEM, 1 μm. TEM, transmission electron microscopy. (k) Representative staining micrographs show renal expression of p16INK4A, SA‐ beta ‐gal activity, and gamma H2AX. Paraffin kidney sections were stained with antibodies against p16INK4A and gamma H2AX. Frozen kidney sections were stained for SA‐ beta ‐gal activity. Arrows indicate positive staining. Scale bar, 25 μm. (l–n) Representative (l) Western blots and graphical representations of (m) p16INK4A and (n) p19ARF protein expression levels in kidneys are presented. *p < .05 versus control mice; †p < .05 versus d‐gal‐treated mice (n = 5–6) Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31318148), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Klotho

Klotho, also called Klotho-alpha, is the founding member of the Klotho family within the glycosidase-1 superfamily. Klotho is expressed in areas concerned with calcium regulation, predominantly in the kidney distal convoluted tubules, but also in the brain choroid plexus (which produces cerebrospinal fluid) and the parathyroid. The 1014 amino acid (aa) type I transmembrane protein contains a 34 aa signal sequence, a 948 aa extracellular domain (ECD) containing two extracellular glycosidase-like domains, a 21 aa transmembrane domain and an 11 aa intracellular domain. Within the ECD, mouse Klotho shares 95%, 87%, and 87% aa identity with rat, human, and equine Klotho, respectively. Although a truncated 554 aa isoform predicts a soluble 70 kDa form, the soluble form found in plasma and cerebrospinal fluid is a 130 kDa form produced by proteolytic cleavage of the glycosylated 135 kDa full-length Klotho. A prominent intracellular 120 kDa form of Klotho is localized to endoplasmic reticulum and Golgi membranes. Klotho is named for the Greek goddess who spins the thread of life. The phenotype of Klotho-deficient mice resembles premature aging, including arteriosclerosis, osteoporosis, skin atrophy, infertility, emphysema, and premature death. Conversely, excess Klotho extends lifespan. Klotho acts as a cofactor for interaction of FGF-23 with FGF R1. This interaction negatively regulates 1 alpha -hydroxylase, the rate-limiting enzyme in the synthesis of 1,25(OH)2D3 (vitamin D). Klotho-deficient mice show severe hyperphosphatemia and ectopic calcification of soft tissues due to excess vitamin D. Both Klotho and beta ‑Klotho are co-factors for FGF-19 binding. Klotho also shows glucuronidase activity which activates the renal ion channel TRPV5 to reabsorb urinary calcium. Klotho has been reported to downregulate insulin or IGF-I signaling in adipocytes, to bind and antagonize Wnt molecules, and to facilitate release of parathyroid hormone.

Entrez Gene IDs
9365 (Human); 16591 (Mouse)
Alternate Names
EC 3.2.1; EC 3.2.1.31; KL; Klotho

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Citations for Mouse Klotho Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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  1. Klotho Regulated by Estrogen Plays a Key Role in Sex Differences in Stress Resilience in Rats
    Authors: Z Tan, Y Li, Y Guan, J Iqbal, C Wang, R Yan, XM Ma
    International Journal of Molecular Sciences, 2023-01-07;24(2):.
    Species: Mouse
    Sample Types: Tissue Lysates, Whole Tissue
    Applications: IHC, Western Blot
  2. Deubiquitinating enzyme USP11 promotes renal tubular cell senescence and fibrosis via inhibiting the ubiquitin degradation of TGF-beta receptor II
    Authors: JY Ni, X Wang, HY Xie, NH Yang, JY Li, XA Sun, HJ Guo, L Zhou, W Zhang, J Liu, LM Lu
    Acta pharmacologica Sinica, 2022-08-31;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. A Klotho-derived peptide protects against kidney fibrosis by targeting TGF-beta signaling
    Authors: Q Yuan, Q Ren, L Li, H Tan, M Lu, Y Tian, L Huang, B Zhao, H Fu, FF Hou, L Zhou, Y Liu
    Nature Communications, 2022-01-21;13(1):438.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  4. Cannabinoid receptor 2 plays a central role in renal tubular mitochondrial dysfunction and kidney ageing
    Authors: S Zhou, X Ling, P Meng, Y Liang, K Shen, Q Wu, Y Zhang, Q Chen, S Chen, Y Liu, L Zhou
    Journal of Cellular and Molecular Medicine, 2021-08-19;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  5. Klotho retards renal fibrosis through targeting mitochondrial dysfunction and cellular senescence in renal tubular cells
    Authors: J Miao, J Huang, C Luo, H Ye, X Ling, Q Wu, W Shen, L Zhou
    Physiological Reports, 2021-01-01;9(2):e14696.
    Species: Human, Mouse
    Sample Types: Cell Lysates, Tissue Homogenates
    Applications: Immunoprecipitation, Western Blot
  6. Salt causes aging-associated hypertension via vascular Wnt5a under Klotho deficiency
    Authors: W Kawarazaki, R Mizuno, M Nishimoto, N Ayuzawa, D Hirohama, K Ueda, F Kawakami-M, S Oba, T Marumo, T Fujita
    J. Clin. Invest., 2020-08-03;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Lysates
    Applications: Immunoprecipitation
  7. Insulin is produced in choroid plexus and its release is regulated by serotonergic signaling
    Authors: CH Mazucanti, QR Liu, D Lang, N Huang, JF O'Connell, S Camandola, JM Egan
    JCI Insight, 2019-12-05;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  8. Wnt/?-catenin/RAS signaling mediates age-related renal fibrosis and is associated with mitochondrial dysfunction
    Authors: J Miao, J Liu, J Niu, Y Zhang, W Shen, C Luo, Y Liu, C Li, H Li, P Yang, Y Liu, FF Hou, L Zhou
    Aging Cell, 2019-07-18;0(0):e13004.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-Fr, IHC-P, Western Blot
  9. Klotho expression is a prerequisite for proper muscle stem cell function and regeneration of skeletal muscle
    Authors: HE Ahrens, J Huettemeis, M Schmidt, C Kaether, J von Maltza
    Skelet Muscle, 2018-07-04;8(1):20.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  10. Secreted Klotho Attenuates Inflammation-Associated Aortic Valve Fibrosis in Senescence-Accelerated Mice P1
    Authors: J Chen, J Fan, S Wang, Z Sun
    Hypertension, 2018-03-26;0(0):.
    Species: Mouse
    Sample Types: Serum
    Applications: Western Blot
  11. Persistent fibroblast growth factor 23 signalling in the parathyroid glands for secondary hyperparathyroidism in mice with chronic kidney disease
    Authors: K Kawakami, A Takeshita, K Furushima, M Miyajima, I Hatamura, M Kuro-O, Y Furuta, K Sakaguchi
    Sci Rep, 2017-01-17;7(0):40534.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  12. Antiaging Gene Klotho Attenuates Pancreatic beta-Cell Apoptosis in Type 1 Diabetes.
    Authors: Lin Y, Sun Z
    Diabetes, 2015-09-04;64(12):4298-311.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  13. Expression of klotho mRNA and protein in rat brain parenchyma from early postnatal development into adulthood.
    Authors: Clinton S, Glover M, Maltare A, Laszczyk A, Mehi S, Simmons R, King G
    Brain Res, 2013-07-06;1527(0):1-14.
    Species: Rat
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC-P
  14. Loss of Klotho contributes to kidney injury by derepression of Wnt/beta-catenin signaling.
    Authors: Zhou L, Li Y, Zhou D, Tan R, Liu Y
    J Am Soc Nephrol, 2013-04-04;24(5):771-85.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells, Whole Tissue
    Applications: ICC, IHC-P, Immunoprecipitation, Western Blot
  15. FGF23 suppresses chondrocyte proliferation in the presence of soluble alpha-Klotho both in vitro and in vivo.
    Authors: Kawai M, Kinoshita S, Kimoto A, Hasegawa Y, Miyagawa K, Yamazaki M, Ohata Y, Ozono K, Michigami T
    J Biol Chem, 2012-12-12;288(4):2414-27.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  16. Circulating alphaKlotho influences phosphate handling by controlling FGF23 production.
    Authors: Smith R
    J. Clin. Invest., 2012-11-26;122(12):4710-5.
    Species: Mouse
    Sample Types: Plasma
    Applications: ELISA Development
  17. Vitamin D receptor agonists increase klotho and osteopontin while decreasing aortic calcification in mice with chronic kidney disease fed a high phosphate diet.
    Authors: Lau, Wei Ling, Leaf, Elizabet, Hu, Ming Cha, Takeno, Marc M, Kuro-o, Makoto, Moe, Orson W, Giachelli, Cecilia
    Kidney Int, 2012-08-29;82(12):1261-70.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC

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Reviews for Mouse Klotho Antibody

Average Rating: 4 (Based on 3 Reviews)

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Mouse Klotho Antibody
By Anonymous on 12/21/2017
Application: WB Sample Tested: HEK293 human embryonic kidney cell line Species: HEK cells and HEK293 cells transfected with mouse klotho

Mouse Klotho Antibody
By Anonymous on 12/02/2016
Application: WB Sample Tested: Kidney tissue Species: Mouse

Mouse Klotho Antibody
By Anonymous on 06/10/2016
Application: WB Sample Tested: Adult kidney Species: Rat