Mouse LDL R Antibody

Catalog # Availability Size / Price Qty
AF2255
AF2255-SP
LDL R in Mouse Liver.
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Product Details
Citations (17)
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Mouse LDL R Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse LDL R in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human LDL R is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse LDL R
Ala22-Arg790 (Ala23Val, Cys27Gly)
Accession # Q6GTJ9
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse LDL R (Catalog # 2255-LD)
Flow Cytometry
2.5 µg/106 cells
Serum‑deprived RAW 264.7 mouse monocyte/macrophage cell line
Immunohistochemistry
5-15 µg/mL
See below
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.04‑0.2 µg/mL of this antibody will block 50% of the binding of 200 ng/mL of  Recombinant Mouse LDL R (Catalog # 2255-LD) to immobilized human Low Density Lipoprotein coated at 2 µg/mL (100 µL/well). At 3 µg/mL, this antibody will block >95% of the binding.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry LDL R in Mouse Liver. View Larger

LDL R in Mouse Liver. LDL R was detected in perfusion fixed frozen sections of mouse liver using Mouse LDL R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2255) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the bile canaliculi. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LDL R

The low density lipoprotein receptor (LDL R) is the founding member of the LDL R family of scavenger receptors (1, 2, 3, 4). This family contains type I transmembrane molecules that are characterized by the presence of EGF repeats, complement-like repeats, and YWTD motifs that form beta -propellers. Although members of the family were originally thought to be endocytic receptors, it is now clear that some members interact with adjacent cell-surface molecules, expanding their range of activities (2, 4). Mouse LDL R is synthesized as a 864 amino acid (aa) precursor that contains a 21 aa signal sequence, a 769 aa extracellular region, a 22 aa transmembrane segment and a 52 aa cytoplasmic tail (5). The extracellular region is complex. It consists of seven N-terminal complement-like cysteine-rich repeats (class A LDL domains) that bind LDL. Cysteines in this region participate in intrachain disulfide bonds. This region is followed by two EGF-like domains and six class B LDL repeats that generate a beta -propeller whose blades each contain a YWTD motif. This area is likely responsible for ligand dissociation (6). Finally, there is a 50 aa membrane proximal Ser/Thr-rich region that shows extensive O-linked glycosylation, generating a native molecular weight for LDL R of 135 kDa (5). Within the 52 aa cytoplasmic region, there is an NPxY motif that links the receptor to clathrin pits and binds to select adaptor proteins (1, 7, 8). The extracellular region of mouse LDL R shares 78% and 87% aa identity with the extracellular region of human and rat LDL R, respectively. LDL R is constitutively expressed and binds apoB of LDL and apoE of VLDL (9). It is responsible for clearing 70% of plasma LDL in liver (9).

References
  1. Strickland, D.K. et al. (2002) Trends Endocrinol. Metab. 13:66.
  2. Nykjaer, A. and T.E. Willnow (2002) Trends Cell Biol. 12:273.
  3. Gent, J. and I. Braakman (2004) Cell. Mol. Life Sci. 61:2461.
  4. Bujo, H. and Y. Saito (2006) Arterioscler. Thromb. Vasc. Biol. 26:1246.
  5. Hoffer, M.J. V. et al. (1993) Biochem. Biophys. Res. Commun. 191:880.
  6. Rudenko, G. and J. Deisenhofer (2003) Curr. Opin. Struct. Biol. 13:683.
  7. Trommsdorff, M. et al. (1998) J. Biol. Chem. 273:33556.
  8. Stolt, P.C. and H.H. Bock (2006) Cell. Signal. 18:1560
  9. Defesche, J.C. (2004) Semin. Vasc. Med. 4:5.
Long Name
Low Density Lipoprotein Receptor
Entrez Gene IDs
3949 (Human); 16835 (Mouse); 300438 (Rat)
Alternate Names
FH; FHC; LDL R; LDL receptor; LDLCQ2; LDLR; low density lipoprotein receptor; low-density lipoprotein receptor class A domain-containing protein 3; low-density lipoprotein receptor

Product Datasheets

Citations for Mouse LDL R Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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  1. Loss-of-function PCSK9 mutants evade the unfolded protein response sensor, GRP78, and fail to induce endoplasmic reticulum stress when retained
    Authors: P Lebeau, K Platko, AA Al-Hashimi, JH Byun, Š Lhoták, N Holzapfel, G Gyulay, SA Igdoura, D Cool, B Trigatti, NG Seidah, RC Austin
    J. Biol. Chem., 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. A wild-type mouse-based model for the regression of inflammation in atherosclerosis
    Authors: M Peled, H Nishi, A Weinstock, TJ Barrett, F Zhou, A Quezada, EA Fisher
    PLoS ONE, 2017;12(3):e0173975.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB
  3. Comparing expression and activity of PCSK9 in SPRET/EiJ and C57BL/6J mouse strains shows lack of correlation with plasma cholesterol
    Mol Genet Metab Rep, 2017;10(0):11-17.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  4. Liver-specific ATP-citrate lyase inhibition by bempedoic acid decreases LDL-C and attenuates atherosclerosis
    Nat Commun, 2016;7(0):13457.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Targeting cancer metabolism by simultaneously disrupting parallel nutrient access pathways
    J Clin Invest, 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC - Fresh
  6. Alirocumab, a Therapeutic Human Antibody to PCSK9, Does Not Affect CD81 Levels or Hepatitis C Virus Entry and Replication into Hepatocytes
    PLoS ONE, 2016;11(4):e0154498.
    Species: Mouse
    Sample Types: Serum
    Applications: WB
  7. Endoplasmic reticulum stress and Ca2+ depletion differentially modulate the sterol-regulatory protein PCSK9 to control lipid metabolism
    Authors: Paul Lebeau
    J. Biol. Chem, 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. Peptide-based anti-PCSK9 vaccines - an approach for long-term LDLc management.
    Authors: Galabova G, Brunner S, Winsauer G, Juno C, Wanko B, Mairhofer A, Luhrs P, Schneeberger A, von Bonin A, Mattner F, Schmidt W, Staffler G
    PLoS ONE, 2014;9(12):e114469.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Development
  9. Pericyte loss influences Alzheimer-like neurodegeneration in mice.
    Authors: Sagare A, Bell R, Zhao Z, Ma Q, Winkler E, Ramanathan A, Zlokovic B
    Nat Commun, 2013;4(0):2932.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Blocking
  10. Annexin A2 Is a Natural Extrahepatic Inhibitor of the PCSK9-Induced LDL Receptor Degradation.
    Authors: Seidah NG, Poirier S, Denis M, Parker R, Miao B, Mapelli C, Prat A, Wassef H, Davignon J, Hajjar KA, Mayer G
    PLoS ONE, 2012;7(7):e41865.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Frozen
  11. In vivo evidence that furin from hepatocytes inactivates PCSK9.
    Authors: Essalmani R, Susan-Resiga D, Chamberland A, Abifadel M, Creemers JW, Boileau C, Seidah NG, Prat A
    J. Biol. Chem., 2011;286(6):4257-63.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB
  12. Apolipoprotein B binding domains: evidence that they are cell-penetrating peptides that efficiently deliver antigenic peptide for cross-presentation of cytotoxic T cells.
    Authors: Sakamoto N, Rosenberg AS,
    J. Immunol., 2011;186(8):5004-11.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow
  13. An ABCA1-independent pathway for recycling a poorly lipidated 8.1 nm apolipoprotein E particle from glia.
    Authors: Fan J, Stukas S, Wong C
    J. Lipid Res., 2011;52(9):1605-16.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: WB
  14. Uptake of dietary retinoids at the maternal-fetal barrier: in vivo evidence for the role of lipoprotein lipase and alternative pathways.
    Authors: Wassef L, Quadro L,
    J. Biol. Chem., 2011;286(37):32198-207.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB
  15. Secreted proprotein convertase subtilisin/kexin type 9 reduces both hepatic and extrahepatic low-density lipoprotein receptors in vivo.
    Authors: Schmidt RJ, Beyer TP, Bensch WR, Qian YW, Lin A, Kowala M, Alborn WE, Konrad RJ, Cao G
    Biochem. Biophys. Res. Commun., 2008;370(4):634-40.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: WB
  16. apoE isoform-specific disruption of amyloid beta peptide clearance from mouse brain.
    Authors: Deane R, Sagare A, Hamm K, Parisi M, Lane S, Finn MB, Holtzman DM, Zlokovic BV
    J. Clin. Invest., 2008;118(12):4002-13.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neut
  17. Embryonic stem cells cultured in serum-free medium acquire bovine apolipoprotein B-100 from feeder cell layers and serum replacement medium.
    Authors: Hisamatsu-Sakamoto M, Sakamoto N, Rosenberg AS
    Stem Cells, 2007;26(1):72-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow

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