Mouse LDLR Antibody

Catalog # Availability Size / Price Qty
AF2255
AF2255-SP
Detection of Mouse LDLR by Western Blot.
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Product Details
Citations (25)
FAQs
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Reviews (1)

Mouse LDLR Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse LDL R in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human LDL R is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse LDL R
Ala22-Arg790 (Ala23Val, Cys27Gly)
Accession # Q6GTJ9
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Flow Cytometry
2.5 µg/106 cells
Serum‑deprived RAW 264.7 mouse monocyte/macrophage cell line
Immunohistochemistry
5-15 µg/mL
See below
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.04‑0.2 µg/mL of this antibody will block 50% of the binding of 200 ng/mL of  Recombinant Mouse LDL R (Catalog # 2255-LD) to immobilized human Low Density Lipoprotein coated at 2 µg/mL (100 µL/well). At 3 µg/mL, this antibody will block >95% of the binding.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse LDLR antibody by Western Blot. View Larger

Detection of Mouse LDLR by Western Blot. Western blot shows lysates of mouse liver tissue. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Mouse LDLR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2255) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for LDLR at approximately 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry LDL R antibody in Mouse Liver by Immunohistochemistry (IHC-Fr). View Larger

LDL R in Mouse Liver. LDL R was detected in perfusion fixed frozen sections of mouse liver using Mouse LDL R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2255) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the bile canaliculi. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Western Blot Detection of Mouse LDLR by Western Blot View Larger

Detection of Mouse LDLR by Western Blot Effect of Pcsk9 deletion on LDLR and CD81 levels in age- and sex-matched Pcsk9-/- mice (n = 5) and their wild type littermates (n = 4).Shown are (A) mean ± SE serum LDL-C levels and (B) Western blot analysis of CD81 and LDLR levels in liver extracts with TfR as loading control, in animals sacrificed after a 4-hour fast. Lanes in panel B represent samples from individual mice. LDL-C, low-density lipoprotein cholesterol; LDLR, low-density lipoprotein receptor; PCSK9, proprotein convertase subtilisin/kexin type 9; SE, standard error; TfR, transferrin receptor; wt, wild type. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0154498), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse LDLR by Western Blot View Larger

Detection of Mouse LDLR by Western Blot Effect of administration of alirocumab (monoclonal antibody to PCSK9) or control antibody (10 mg/kg) on LDLR and CD81 levels in hyperlipidemic Pcsk9hum/humLdlr+/- mice.Shown are mean ± SE serum LDL-C levels (A) and Western blot analysis of CD81 and LDLR levels in liver extracts using GAPDH as loading control (B). Western blot in B was quantified using Image J. The intensities of CD81 and LDLR bands were adjusted to respective loading control for each lane and presented as a fold change from control antibody treated group. Means ± SE are shown. Serum and livers were collected on Day 4 after antibody administration. In panel B, the three columns represent three livers collected for each of the two treatments. Ab, antibody; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; LDL-C, low-density lipoprotein cholesterol; LDLR, low-density lipoprotein receptor; mAb, monoclonal Ab. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0154498), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse LDLR by Western Blot View Larger

Detection of Mouse LDLR by Western Blot Hypercholesterolemia induced by recombinant Adeno-Associated Virus (rAAV)8-D377Y-murine Proprotein Convertase Subtilisin/Kexin type 9 (mPCSK9) in chow diet-fed mice.A. Serum levels of murine PCSK9 protein in mice injected with AAVmPCSK9 virus, AAVnull–injected or saline-injected control mice. **P<0.01. B. Liver LDL receptor protein levels analyzed by western blot and normalized to alpha -tubulin (n = 3). C. Plasma total cholesterol of chow diet—fed mice. **P<0.01 compared with control, ***P<0.01 compared with control and AAVmPCSK9. D. Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured at the same time point and determined as described in Materials and Methods. Values are mean ± SEM; n = 3. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28291840), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LDLR

The low density lipoprotein receptor (LDL R) is the founding member of the LDL R family of scavenger receptors (1, 2, 3, 4). This family contains type I transmembrane molecules that are characterized by the presence of EGF repeats, complement-like repeats, and YWTD motifs that form beta -propellers. Although members of the family were originally thought to be endocytic receptors, it is now clear that some members interact with adjacent cell-surface molecules, expanding their range of activities (2, 4). Mouse LDL R is synthesized as a 864 amino acid (aa) precursor that contains a 21 aa signal sequence, a 769 aa extracellular region, a 22 aa transmembrane segment and a 52 aa cytoplasmic tail (5). The extracellular region is complex. It consists of seven N-terminal complement-like cysteine-rich repeats (class A LDL domains) that bind LDL. Cysteines in this region participate in intrachain disulfide bonds. This region is followed by two EGF-like domains and six class B LDL repeats that generate a beta -propeller whose blades each contain a YWTD motif. This area is likely responsible for ligand dissociation (6). Finally, there is a 50 aa membrane proximal Ser/Thr-rich region that shows extensive O-linked glycosylation, generating a native molecular weight for LDL R of 135 kDa (5). Within the 52 aa cytoplasmic region, there is an NPxY motif that links the receptor to clathrin pits and binds to select adaptor proteins (1, 7, 8). The extracellular region of mouse LDL R shares 78% and 87% aa identity with the extracellular region of human and rat LDL R, respectively. LDL R is constitutively expressed and binds apoB of LDL and apoE of VLDL (9). It is responsible for clearing 70% of plasma LDL in liver (9).

References
  1. Strickland, D.K. et al. (2002) Trends Endocrinol. Metab. 13:66.
  2. Nykjaer, A. and T.E. Willnow (2002) Trends Cell Biol. 12:273.
  3. Gent, J. and I. Braakman (2004) Cell. Mol. Life Sci. 61:2461.
  4. Bujo, H. and Y. Saito (2006) Arterioscler. Thromb. Vasc. Biol. 26:1246.
  5. Hoffer, M.J. V. et al. (1993) Biochem. Biophys. Res. Commun. 191:880.
  6. Rudenko, G. and J. Deisenhofer (2003) Curr. Opin. Struct. Biol. 13:683.
  7. Trommsdorff, M. et al. (1998) J. Biol. Chem. 273:33556.
  8. Stolt, P.C. and H.H. Bock (2006) Cell. Signal. 18:1560
  9. Defesche, J.C. (2004) Semin. Vasc. Med. 4:5.
Long Name
Low Density Lipoprotein Receptor
Entrez Gene IDs
3949 (Human); 16835 (Mouse); 300438 (Rat); 396801 (Porcine); 102127361 (Cynomolgus Monkey)
Alternate Names
FH; FHC; LDL R; LDL receptor; LDLCQ2; LDLR; low density lipoprotein receptor; low-density lipoprotein receptor class A domain-containing protein 3; low-density lipoprotein receptor

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Citations for Mouse LDLR Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

25 Citations: Showing 1 - 10
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  1. Delivery of low-density lipoprotein from endocytic carriers to mitochondria supports steroidogenesis
    Authors: Zhou, YX;Wei, J;Deng, G;Hu, A;Sun, PY;Zhao, X;Song, BL;Luo, J;
    Nature cell biology
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells, Whole Tissue
    Applications: ICC, IHC, Western Blot
  2. A Virus-like particle-based bivalent PCSK9 vaccine lowers LDL-cholesterol levels in Non-Human Primates
    Authors: Fowler, A;Van Rompay, KKA;Sampson, M;Leo, J;Watanabe, JK;Usachenko, JL;Immareddy, R;Lovato, DM;Schiller, JT;Remaley, AT;Chackerian, B;
    bioRxiv : the preprint server for biology
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. Accumulation of cholesterol, triglycerides and ceramides in hepatocellular carcinomas of diethylnitrosamine injected mice
    Authors: EM Haberl, R Pohl, L Rein-Fisch, M Höring, S Krautbauer, G Liebisch, C Buechler
    Lipids in Health and Disease, 2021-10-10;20(1):135.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  4. An original infection model identifies host lipoprotein import as a route for blood-brain barrier crossing
    Authors: B Benmimoun, F Papastefan, B Périchon, K Segklia, N Roby, V Miriagou, C Schmitt, S Dramsi, R Matsas, P Spéder
    Nature Communications, 2020-11-30;11(1):6106.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Liver Pyruvate Kinase Promotes NAFLD/NASH in both Mice and Humans in a Sex-Specific Manner
    Authors: KC Krishnan, RR Floyd, S Sabir, DW Jayasekera, PV Leon-Mimil, AE Jones, AA Cortez, V Shravah, M Péterfy, L Stiles, S Canizales-, AS Divakaruni, A Huertas-Va, AJ Lusis
    Cell Mol Gastroenterol Hepatol, 2020-09-14;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Development of Novel DNA-Encoded PCSK9 Monoclonal Antibodies as Lipid-Lowering Therapeutics
    Authors: M Khoshnejad, A Patel, K Wojtak, SB Kudchodkar, L Humeau, NN Lyssenko, DJ Rader, K Muthumani, DB Weiner
    Mol. Ther., 2018-11-15;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  7. The adaptor protein PID1 regulates receptor-dependent endocytosis of postprandial triglyceride-rich lipoproteins
    Authors: AW Fischer, K Albers, LM Krott, B Hoffzimmer, M Heine, H Schmale, L Scheja, PLSM Gordts, J Heeren
    Mol Metab, 2018-07-30;16(0):88-99.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Loss-of-function PCSK9 mutants evade the unfolded protein response sensor, GRP78, and fail to induce endoplasmic reticulum stress when retained
    Authors: P Lebeau, K Platko, AA Al-Hashimi, JH Byun, Š Lhoták, N Holzapfel, G Gyulay, SA Igdoura, D Cool, B Trigatti, NG Seidah, RC Austin
    J. Biol. Chem., 2018-03-28;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. A wild-type mouse-based model for the regression of inflammation in atherosclerosis
    Authors: M Peled, H Nishi, A Weinstock, TJ Barrett, F Zhou, A Quezada, EA Fisher
    PLoS ONE, 2017-03-14;12(3):e0173975.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  10. Comparing expression and activity of PCSK9 in SPRET/EiJ and C57BL/6J mouse strains shows lack of correlation with plasma cholesterol
    Mol Genet Metab Rep, 2016-12-10;10(0):11-17.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. Endoplasmic reticulum stress and Ca2+ depletion differentially modulate the sterol-regulatory protein PCSK9 to control lipid metabolism
    Authors: Paul Lebeau
    J. Biol. Chem, 2016-12-01;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  12. Liver-specific ATP-citrate lyase inhibition by bempedoic acid decreases LDL-C and attenuates atherosclerosis
    Nat Commun, 2016-11-28;7(0):13457.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  13. Targeting cancer metabolism by simultaneously disrupting parallel nutrient access pathways
    J Clin Invest, 2016-09-26;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC-Fr
  14. Alirocumab, a Therapeutic Human Antibody to PCSK9, Does Not Affect CD81 Levels or Hepatitis C Virus Entry and Replication into Hepatocytes
    PLoS ONE, 2016-04-26;11(4):e0154498.
    Species: Mouse
    Sample Types: Serum
    Applications: Western Blot
  15. Peptide-based anti-PCSK9 vaccines - an approach for long-term LDLc management.
    Authors: Galabova G, Brunner S, Winsauer G, Juno C, Wanko B, Mairhofer A, Luhrs P, Schneeberger A, von Bonin A, Mattner F, Schmidt W, Staffler G
    PLoS ONE, 2014-12-04;9(12):e114469.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Development
  16. Hormonal modulators of glial ABCA1 and apoE levels.
    Authors: Fan, Jianjia, Shimizu, Yoko, Chan, Jeniffer, Wilkinson, Anna, Ito, Ayaka, Tontonoz, Peter, Dullaghan, Edie, Galea, Liisa A, Pfeifer, Tom, Wellington, Cheryl L
    J Lipid Res, 2013-09-02;54(11):3139-50.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  17. Pericyte loss influences Alzheimer-like neurodegeneration in mice.
    Authors: Sagare A, Bell R, Zhao Z, Ma Q, Winkler E, Ramanathan A, Zlokovic B
    Nat Commun, 2013-01-01;4(0):2932.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Blocking
  18. Annexin A2 Is a Natural Extrahepatic Inhibitor of the PCSK9-Induced LDL Receptor Degradation.
    Authors: Seidah NG, Poirier S, Denis M, Parker R, Miao B, Mapelli C, Prat A, Wassef H, Davignon J, Hajjar KA, Mayer G
    PLoS ONE, 2012-07-27;7(7):e41865.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  19. Uptake of dietary retinoids at the maternal-fetal barrier: in vivo evidence for the role of lipoprotein lipase and alternative pathways.
    Authors: Wassef L, Quadro L
    J. Biol. Chem., 2011-07-27;286(37):32198-207.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  20. An ABCA1-independent pathway for recycling a poorly lipidated 8.1 nm apolipoprotein E particle from glia.
    Authors: Fan J, Stukas S, Wong C
    J. Lipid Res., 2011-06-26;52(9):1605-16.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  21. Apolipoprotein B binding domains: evidence that they are cell-penetrating peptides that efficiently deliver antigenic peptide for cross-presentation of cytotoxic T cells.
    Authors: Sakamoto N, Rosenberg AS
    J. Immunol., 2011-03-14;186(8):5004-11.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  22. In vivo evidence that furin from hepatocytes inactivates PCSK9.
    Authors: Essalmani R, Susan-Resiga D, Chamberland A, Abifadel M, Creemers JW, Boileau C, Seidah NG, Prat A
    J. Biol. Chem., 2010-12-08;286(6):4257-63.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  23. apoE isoform-specific disruption of amyloid beta peptide clearance from mouse brain.
    Authors: Deane R, Sagare A, Hamm K, Parisi M, Lane S, Finn MB, Holtzman DM, Zlokovic BV
    J. Clin. Invest., 2008-11-13;118(12):4002-13.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  24. Secreted proprotein convertase subtilisin/kexin type 9 reduces both hepatic and extrahepatic low-density lipoprotein receptors in vivo.
    Authors: Schmidt RJ, Beyer TP, Bensch WR, Qian YW, Lin A, Kowala M, Alborn WE, Konrad RJ, Cao G
    Biochem. Biophys. Res. Commun., 2008-04-10;370(4):634-40.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  25. Embryonic stem cells cultured in serum-free medium acquire bovine apolipoprotein B-100 from feeder cell layers and serum replacement medium.
    Authors: Hisamatsu-Sakamoto M, Sakamoto N, Rosenberg AS
    Stem Cells, 2007-10-18;26(1):72-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Mouse LDL R Antibody
By Boyan Zhang on 01/30/2019
Application: WB Sample Tested: MEF Species: Mouse

When the cells were lipid-depleted (N.L.), the protein level of endogenous LDLR was significantly increased (shown in the figure).