Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse, Rat, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Blockade of Receptor-ligand Interaction, Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Neutralization, Flow Cytometry, Immunocytochemistry, Blocking, ELISA Development

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all LDLR Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse LDL R
Ala22-Arg790 (Ala23Val, Cys27Gly)
Accession # Q6GTJ9

Specificity

Detects mouse LDL R in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human LDL R is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse LDLR Antibody

Detection of Mouse LDLR antibody by Western Blot.

Detection of Mouse LDLR by Western Blot.

Western blot shows lysates of mouse liver tissue. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Mouse LDLR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2255) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for LDLR at approximately 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
LDL R antibody in Mouse Liver by Immunohistochemistry (IHC-Fr).

LDL R in Mouse Liver.

LDL R was detected in perfusion fixed frozen sections of mouse liver using Mouse LDL R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2255) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the bile canaliculi. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Detection of Mouse LDLR by Western Blot

Detection of Mouse LDLR by Western Blot

Effect of Pcsk9 deletion on LDLR and CD81 levels in age- and sex-matched Pcsk9-/- mice (n = 5) and their wild type littermates (n = 4).Shown are (A) mean ± SE serum LDL-C levels and (B) Western blot analysis of CD81 and LDLR levels in liver extracts with TfR as loading control, in animals sacrificed after a 4-hour fast. Lanes in panel B represent samples from individual mice. LDL-C, low-density lipoprotein cholesterol; LDLR, low-density lipoprotein receptor; PCSK9, proprotein convertase subtilisin/kexin type 9; SE, standard error; TfR, transferrin receptor; wt, wild type. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0154498), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse LDLR by Western Blot

Detection of Mouse LDLR by Western Blot

Effect of administration of alirocumab (monoclonal antibody to PCSK9) or control antibody (10 mg/kg) on LDLR and CD81 levels in hyperlipidemic Pcsk9hum/humLdlr+/- mice.Shown are mean ± SE serum LDL-C levels (A) and Western blot analysis of CD81 and LDLR levels in liver extracts using GAPDH as loading control (B). Western blot in B was quantified using Image J. The intensities of CD81 and LDLR bands were adjusted to respective loading control for each lane and presented as a fold change from control antibody treated group. Means ± SE are shown. Serum and livers were collected on Day 4 after antibody administration. In panel B, the three columns represent three livers collected for each of the two treatments. Ab, antibody; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; LDL-C, low-density lipoprotein cholesterol; LDLR, low-density lipoprotein receptor; mAb, monoclonal Ab. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0154498), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse LDLR by Western Blot

Detection of Mouse LDLR by Western Blot

Hypercholesterolemia induced by recombinant Adeno-Associated Virus (rAAV)8-D377Y-murine Proprotein Convertase Subtilisin/Kexin type 9 (mPCSK9) in chow diet-fed mice.A. Serum levels of murine PCSK9 protein in mice injected with AAVmPCSK9 virus, AAVnull–injected or saline-injected control mice. **P<0.01. B. Liver LDL receptor protein levels analyzed by western blot and normalized to alpha -tubulin (n = 3). C. Plasma total cholesterol of chow diet—fed mice. **P<0.01 compared with control, ***P<0.01 compared with control and AAVmPCSK9. D. Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured at the same time point and determined as described in Materials and Methods. Values are mean ± SEM; n = 3. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28291840), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse LDLR by Western Blot

Detection of Mouse LDLR by Western Blot

Lymphatic function assessment in mice following a specific knockdown of LDLR in murine endothelial cells. Two weeks after the intraperitoneal injection of an adeno-associated virus type 1 (AAV1) containing a shRNA targeting LDLR, skin draining lymph nodes were harvested, digested and analyzed by flow cytometry to assess lymphatic endothelial cell (LEC)-specific knockdown of LDLR expression in mice. Membrane-bound LDLR expression was measured on (A) CD45-CD31+Podoplanin+ and (B) CD45+ cells. Fluorescence minus one (FMO) control was used for LDLR expression, as depicted by the dotted line in the first histogram. White histogram, shSCR and dark histogram, shLldlr. LDLR expression was also determine by immunoblotting in (C) aorta isolated from female mice and (D) in liver from wild-type and Pcsk9-/- male (full dot) and female (empty dot) mice. ShSCR in black and ShLdlr in grey. Total plasmatic cholesterol was measured following FPLC in each liproprotein subfraction in (E) wild-type and (F)Pcsk9-/-female (dotted lines) and male (solid lines) mice treated with shSCR (black lines) and shLdlr (grey lines). Lymphatic contraction capacity was assessed by fluorescent in vivo imaging in (G) wild-type and (H)Pcsk9-/- mice. Black histogram, ShSCR and grey histogram, ShLdlr. n=3-9. Statistics **p < 0,01. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35154499), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse LDLR by Western Blot

Detection of Mouse LDLR by Western Blot

Lymphatic function assessment in mice following a specific knockdown of LDLR in murine endothelial cells. Two weeks after the intraperitoneal injection of an adeno-associated virus type 1 (AAV1) containing a shRNA targeting LDLR, skin draining lymph nodes were harvested, digested and analyzed by flow cytometry to assess lymphatic endothelial cell (LEC)-specific knockdown of LDLR expression in mice. Membrane-bound LDLR expression was measured on (A) CD45-CD31+Podoplanin+ and (B) CD45+ cells. Fluorescence minus one (FMO) control was used for LDLR expression, as depicted by the dotted line in the first histogram. White histogram, shSCR and dark histogram, shLldlr. LDLR expression was also determine by immunoblotting in (C) aorta isolated from female mice and (D) in liver from wild-type and Pcsk9-/- male (full dot) and female (empty dot) mice. ShSCR in black and ShLdlr in grey. Total plasmatic cholesterol was measured following FPLC in each liproprotein subfraction in (E) wild-type and (F)Pcsk9-/-female (dotted lines) and male (solid lines) mice treated with shSCR (black lines) and shLdlr (grey lines). Lymphatic contraction capacity was assessed by fluorescent in vivo imaging in (G) wild-type and (H)Pcsk9-/- mice. Black histogram, ShSCR and grey histogram, ShLdlr. n=3-9. Statistics **p < 0,01. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35154499), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse LDLR Antibody

Application
Recommended Usage

Blockade of Receptor-ligand Interaction

In a functional ELISA, 0.04‑0.2 µg/mL of this antibody will block 50% of the binding of 200 ng/mL of  Recombinant Mouse LDL R (Catalog # 2255-LD) to immobilized human Low Density Lipoprotein coated at 2 µg/mL (100 µL/well). At 3 µg/mL, this antibody will block >95% of the binding.

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Serum‑deprived RAW 264.7 mouse monocyte/macrophage cell line

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse liver

Western Blot

0.1 µg/mL
Sample: Mouse Liver Tissue

Reviewed Applications

Read 1 review rated 5 using AF2255 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LDLR

The low density lipoprotein receptor (LDL R) is the founding member of the LDL R family of scavenger receptors (1, 2, 3, 4). This family contains type I transmembrane molecules that are characterized by the presence of EGF repeats, complement-like repeats, and YWTD motifs that form beta -propellers. Although members of the family were originally thought to be endocytic receptors, it is now clear that some members interact with adjacent cell-surface molecules, expanding their range of activities (2, 4). Mouse LDL R is synthesized as a 864 amino acid (aa) precursor that contains a 21 aa signal sequence, a 769 aa extracellular region, a 22 aa transmembrane segment and a 52 aa cytoplasmic tail (5). The extracellular region is complex. It consists of seven N-terminal complement-like cysteine-rich repeats (class A LDL domains) that bind LDL. Cysteines in this region participate in intrachain disulfide bonds. This region is followed by two EGF-like domains and six class B LDL repeats that generate a beta -propeller whose blades each contain a YWTD motif. This area is likely responsible for ligand dissociation (6). Finally, there is a 50 aa membrane proximal Ser/Thr-rich region that shows extensive O-linked glycosylation, generating a native molecular weight for LDL R of 135 kDa (5). Within the 52 aa cytoplasmic region, there is an NPxY motif that links the receptor to clathrin pits and binds to select adaptor proteins (1, 7, 8). The extracellular region of mouse LDL R shares 78% and 87% aa identity with the extracellular region of human and rat LDL R, respectively. LDL R is constitutively expressed and binds apoB of LDL and apoE of VLDL (9). It is responsible for clearing 70% of plasma LDL in liver (9).

References

  1. Strickland, D.K. et al. (2002) Trends Endocrinol. Metab. 13:66.
  2. Nykjaer, A. and T.E. Willnow (2002) Trends Cell Biol. 12:273.
  3. Gent, J. and I. Braakman (2004) Cell. Mol. Life Sci. 61:2461.
  4. Bujo, H. and Y. Saito (2006) Arterioscler. Thromb. Vasc. Biol. 26:1246.
  5. Hoffer, M.J. V. et al. (1993) Biochem. Biophys. Res. Commun. 191:880.
  6. Rudenko, G. and J. Deisenhofer (2003) Curr. Opin. Struct. Biol. 13:683.
  7. Trommsdorff, M. et al. (1998) J. Biol. Chem. 273:33556.
  8. Stolt, P.C. and H.H. Bock (2006) Cell. Signal. 18:1560
  9. Defesche, J.C. (2004) Semin. Vasc. Med. 4:5.

Long Name

Low Density Lipoprotein Receptor

Alternate Names

LDL R

Entrez Gene IDs

3949 (Human); 16835 (Mouse); 300438 (Rat); 396801 (Porcine); 102127361 (Cynomolgus Monkey)

Gene Symbol

LDLR

UniProt

Q6GTJ9

Additional LDLR Products

Product Documents for Mouse LDLR Antibody

Certificate of Analysis

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Product Specific Notices for Mouse LDLR Antibody

For research use only

Related Research Areas

Obesity

Citations for Mouse LDLR Antibody

Customer Reviews for Mouse LDLR Antibody (1)

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  • Mouse LDL R Antibody
    Name: Boyan Zhang
    Application: Western Blot
    Sample Tested: MEF
    Species: Mouse
    Verified Customer | Posted 01/30/2019
    When the cells were lipid-depleted (N.L.), the protein level of endogenous LDLR was significantly increased (shown in the figure).
    Mouse LDLR Antibody AF2255

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Associated Pathways

A-beta Pathways: Uptake & Degradation A-beta Pathways: Uptake & Degradation Thumbnail