Mouse LIGHT/TNFSF14 Antibody

Catalog # Availability Size / Price Qty
AF1794
AF1794-SP
Detection of Mouse LIGHT/TNFSF14 by Immunocytochemistry/Immunofluorescence
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Product Details
Citations (2)
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Reviews (1)

Mouse LIGHT/TNFSF14 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse LIGHT in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human LIGHT is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse LIGHT (R&D Systems, Catalog # 1794-LT)
Asp72-Val239
Accession # Q9QYH9
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse LIGHT/TNFSF14 (Catalog # 1794-LT)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry/ Immunofluorescence Detection of Mouse LIGHT/TNFSF14 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse LIGHT/TNFSF14 by Immunocytochemistry/Immunofluorescence Concurrent immunofluorescence staining of lymphocytes and LIGHT: Representative images of CD4+ staining (A,D red), LIGHT positive staining (B,E green) and co-expression (C,F yellow). DAPI was utilized as a nuclear counterstain (blue). Upper panels at low power identify an area of CD4+ TIL infiltration in a resected colorectal liver metastasis (200x). Lower panels demonstrate a single high power field in a peritumoral specimen (400x). Intratumoral CD3+ (14 ± 2.08 vs. 2.33 ± .67, p = .00006), CD4+ (8 ± 1.86 vs. 1.67 ± 0.33, p = .0009) and CD8+ (7 ± 2.33 vs. 2.0 ± 0.58, p = .029) lymphocytes were decreased in CRLM compared to lymphocytes from corresponding and equal areas of healthy control liver. Total CD3 + LIGHT + (0.33 ± 0.33 vs. 9 ± 2.65, p = .00006), CD4 + LIGHT + (0. 33 ± 0.33 vs. 5.33 ± .88, p = .0009) and CD8 + LIGHT + (1.33 ± .67 vs. 3.67 ± 1.33, p = .029) cells were decreased in tumor bearing liver compared to control. LIGHT-expressing CD3+ (6.33 ± 2.40 vs. 0.33 ± .33, p = .00006), CD4+ (5.33 ± 1.20 vs. 0.33 ± .33, p = .0009), and CD8+ (5.67 ± 2.40 vs. 1.67 ± 0.33, p = .029) lymphocytes were significantly higher in the peritumor region compared to the intratumor region (panels G-I)(n = 6). Image collected and cropped by CiteAb from the following publication (https://translational-medicine.biomedcentral.com/articles/10.1186/1479-5876-11-70), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Mouse LIGHT/TNFSF14 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse LIGHT/TNFSF14 by Immunocytochemistry/Immunofluorescence Concurrent immunofluorescence staining of lymphocytes and LIGHT: Representative images of CD4+ staining (A,D red), LIGHT positive staining (B,E green) and co-expression (C,F yellow). DAPI was utilized as a nuclear counterstain (blue). Upper panels at low power identify an area of CD4+ TIL infiltration in a resected colorectal liver metastasis (200x). Lower panels demonstrate a single high power field in a peritumoral specimen (400x). Intratumoral CD3+ (14 ± 2.08 vs. 2.33 ± .67, p = .00006), CD4+ (8 ± 1.86 vs. 1.67 ± 0.33, p = .0009) and CD8+ (7 ± 2.33 vs. 2.0 ± 0.58, p = .029) lymphocytes were decreased in CRLM compared to lymphocytes from corresponding and equal areas of healthy control liver. Total CD3 + LIGHT + (0.33 ± 0.33 vs. 9 ± 2.65, p = .00006), CD4 + LIGHT + (0. 33 ± 0.33 vs. 5.33 ± .88, p = .0009) and CD8 + LIGHT + (1.33 ± .67 vs. 3.67 ± 1.33, p = .029) cells were decreased in tumor bearing liver compared to control. LIGHT-expressing CD3+ (6.33 ± 2.40 vs. 0.33 ± .33, p = .00006), CD4+ (5.33 ± 1.20 vs. 0.33 ± .33, p = .0009), and CD8+ (5.67 ± 2.40 vs. 1.67 ± 0.33, p = .029) lymphocytes were significantly higher in the peritumor region compared to the intratumor region (panels G-I)(n = 6). Image collected and cropped by CiteAb from the following publication (https://translational-medicine.biomedcentral.com/articles/10.1186/1479-5876-11-70), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LIGHT/TNFSF14

LIGHT (lymphotoxin-like, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes) is a member of the TNF superfamily and is designated TNFSF14. The gene for mouse LIGHT encodes a 239 amino acid residue (aa) type II transmembrane glycoprotein that contains a 37 aa N-terminal cytoplasmic domain, a 21 aa transmembrane region, and a 181 aa extracellular domain. A soluble form of mouse LIGHT is generated from the membrane form by proteolytic processing. Similar to other TNF ligand family members, LIGHT is assembled as a homotrimer. Mouse and human LIGHT share 71% aa sequence identity.

LIGHT is expressed by activated lymphocytes, natural killer cells, immature dendritic cells, monocytes and granulocytes. Mouse LIGHT binds and signals via two distinct TNF receptor superfamily members, including the herpes virus entry mediator (HVEM/TNFRSF14) and the lymphotoxin beta receptor (LT beta R/TNFRSF3). In humans, LIGHT also binds the soluble human decoy receptor 3 (DcR3/TNFRSF6B). Signaling from LT beta R, which also binds LT alpha beta, induces apoptosis and the production of various cytokines. LIGHT-LT beta R signaling also plays a role in mesenteric lymph node organogenesis, and restoration of secondary lymphoid structure and function. Signaling from HVEM, which also binds LT alpha, co-stimulates T-helper cell type 1 (TH1) immune responses, enhances Cytotoxic T Lymphocytes (CTL)-mediated tumor immunity, and regulates allogeneic T cell activation and allograft rejection. Blockade of LIGHT-HVEM signaling has been shown to prevent graft versus host disease.

References
  1. Granger, S.W. and S. Rickert (2003) Cytokine Growth Factor Rev. 14:289.
Long Name
TNF Ligand Superfamily Member 14
Entrez Gene IDs
8740 (Human); 50930 (Mouse)
Alternate Names
CD258 antigen; CD258; delta transmembrane LIGHT; Herpes virus entry mediator ligand; Herpesvirus entry mediator ligand; HVEM-L; HVEMLherpesvirus entry mediator-ligand; ligand for herpesvirus entry mediator; LIGHT; LIGHTherpesvirus entry mediator A; LTg; TNFSF14; TR2; tumor necrosis factor (ligand) superfamily, member 14; tumor necrosis factor ligand superfamily member 14; tumor necrosis factor receptor-like 2; tumor necrosis factor superfamily member LIGHT

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Citations for Mouse LIGHT/TNFSF14 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Shedding LIGHT (TNFSF14) on the tumor microenvironment of colorectal cancer liver metastases.
    Authors: Qin, Jian Zho, Upadhyay, Vivek, Prabhakar, Bellur, Maker, Ajay V
    J Transl Med, 2013-03-20;11(0):70.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. FN-gamma triggers a LIGHT-dependent selective death of motoneurons contributing to the non-cell-autonomous effects of mutant SOD1.
    Authors: Aebischer J, Cassina P, Otsmane B, Moumen A, Seilhean D, Meininger V, Barbeito L, Pettmann B, Raoul C
    Cell Death Differ., 2010-11-12;18(5):754-68.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization

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Mouse LIGHT/TNFSF14 Antibody
By Anonymous on 10/26/2015
Application: WB Sample Tested: Mouse peritoneal WF-3 cells infected with viral vectors expressing LIGHT.

Specificity: Reasonably specific
Sensitivity: Reasonably sensitive
Buffer: TBSE+0.1% NP40+3% BSA
Dilution: 1:1000