Mouse Mer Antibody

(23 citations)
(1 Review)
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Mer in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross‑reactivity with recombinant human Mer is observed and less than 1% cross-reactivity with recombinant mouse (rm) Axl and rmDtk is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Mer
    Glu23-Phe498
    Accession # Q60805
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse Mer Fc Chimera (Catalog # 591-MR)
  • Flow Cytometry
    2.5 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mer in J774A.1 Mouse Cell Line by Flow Cytometry.

J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Goat Anti-Mouse Mer Antigen Affinity-purified Polyclonal Antibody (Catalog # AF591, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Mer

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt) and Mer (human and mouse homologues of chicken c-Eyk) constitute a receptor tyrosine kinase subfamily. The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. These receptors bind the vitamin K-dependent protein growth-arrest-specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration or the prevention of apoptosis. Studies suggest that this family of tyrosine kinase receptors may be involved in hematopoiesis, embryonic development, tumorigenesis and regulation of testicular functions (1, 2).

  • References:
    1. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
    2. Crosier, K.E. and P.S Crosier (1997) Pathology 29:131.
  • Long Name:
    Receptor Tyrosine Protein Kinase Mer
  • Entrez Gene IDs:
    10461 (Human); 17289 (Mouse)
  • Alternate Names:
    c-Eyk; c-mer proto-oncogene tyrosine kinase; C-mer; EC 2.7.10; EC 2.7.10.1; MER receptor tyrosine kinase; Mer; MerTK; MGC133349; Receptor tyrosine kinase MerTK; RP38Proto-oncogene c-Mer; STK kinase; tyrosine-protein kinase Mer
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

23 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Schwann cells use TAM receptor-mediated phagocytosis in addition to autophagy to clear myelin in a mouse model of nerve injury
    Authors: A Brosius Lu, WS Chung, SA Sloan, GA Carson, L Zhou, E Lovelett, S Posada, JB Zuchero, BA Barres
    Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  2. Macrophage deficiency of miR-21 promotes apoptosis, plaque necrosis, and vascular inflammation during atherogenesis
    Authors: A Canfrán-Du, N Rotllan, X Zhang, M Fernández-, C Ramírez-Hi, E Araldi, L Daimiel, R Busto, C Fernández-, Y Suárez
    EMBO Mol Med, 2017;0(0):.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  3. Sleep Loss Promotes Astrocytic Phagocytosis and Microglial Activation in Mouse Cerebral Cortex
    Authors: M Bellesi, L de Vivo, M Chini, F Gilli, G Tononi, C Cirelli
    J. Neurosci., 2017;37(21):5263-5273.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  4. GAS6 is a key homeostatic immunological regulator of host-commensal interactions in the oral mucosa
    Authors: M Nassar, Y Tabib, T Capucha, G Mizraji, T Nir, M Pevsner-Fi, G Zilberman-, O Heyman, G Nussbaum, H Bercovier, A Wilensky, E Elinav, T Burstyn-Co, AH Hovav
    Proc. Natl. Acad. Sci. U.S.A, 2017;0(0):.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  5. TAM receptors regulate multiple features of microglial physiology
    Authors: L Fourgeaud, PG Través, Y Tufail, H Leal-Baile, ED Lew, PG Burrola, P Callaway, A Zagórska, CV Rothlin, A Nimmerjahn, G Lemke
    Nature, 2016;532(7598):240-4.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  6. MicroRNA-126 overexpression rescues diabetes-induced impairment in efferocytosis of apoptotic cardiomyocytes
    Sci Rep, 2016;6(0):36207.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: IP
  7. MerTK cleavage limits proresolving mediator biosynthesis and exacerbates tissue inflammation
    Proc Natl Acad Sci USA, 2016;0(0):.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  8. Liver X receptor and STAT1 cooperate downstream of Gas6/Mer to induce anti-inflammatory arginase 2 expression in macrophages
    Sci Rep, 2016;6(0):29673.
    Species: Mouse
    Sample Type: In vitro
    Application: in vitro
  9. Apolipoprotein E promotes subretinal mononuclear phagocyte survival and chronic inflammation in age-related macular degeneration.
    Authors: Levy O, Calippe B, Lavalette S, Hu S, Raoul W, Dominguez E, Housset M, Paques M, Sahel J, Bemelmans A, Combadiere C, Guillonneau X, Sennlaub F
    EMBO Mol Med, 2015;7(2):211-26.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  10. Collecting lymphatic vessel permeability facilitates adipose tissue inflammation and distribution of antigen to lymph node-homing adipose tissue dendritic cells.
    Authors: Kuan E, Ivanov S, Bridenbaugh E, Victora G, Wang W, Childs E, Platt A, Jakubzick C, Mason R, Gashev A, Nussenzweig M, Swartz M, Dustin M, Zawieja D, Randolph G
    J Immunol, 2015;194(11):5200-10.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  11. Ly6Chi monocyte recruitment is responsible for Th2 associated host-protective macrophage accumulation in liver inflammation due to schistosomiasis.
    Authors: Nascimento M, Huang S, Smith A, Everts B, Lam W, Bassity E, Gautier E, Randolph G, Pearce E
    PLoS Pathog, 2014;10(8):e1004282.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  12. Diversification of TAM receptor tyrosine kinase function.
    Authors: Zagorska A, Traves P, Lew E, Dransfield I, Lemke G
    Nat Immunol, 2014;15(10):920-8.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: IP
  13. A new mouse model for female genital schistosomiasis.
    Authors: Richardson, Monica L, Fu, Chi-Ling, Pennington, Luke F, Honeycutt, Jared D, Odegaard, Justin I, Hsieh, Yi-Ju, Hammam, Olfat, Conti, Simon L, Hsieh, Michael
    PLoS Negl Trop Dis, 2014;8(5):e2825.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: Flow
  14. Minimal differentiation of classical monocytes as they survey steady-state tissues and transport antigen to lymph nodes.
    Authors: Jakubzick C, Gautier E, Gibbings S, Sojka D, Schlitzer A, Johnson T, Ivanov S, Duan Q, Bala S, Condon T, Van Rooijen N, Grainger J, Belkaid Y, Ma'ayan A, Riches D, Yokoyama W, Ginhoux F, Henson P, Randolph G
    Immunity, 2013;39(3):599-610.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  15. Cross-phosphorylation, signaling and proliferative functions of the Tyro3 and Axl receptors in Rat2 cells.
    Authors: Brown JE, Krodel M, Pazos M
    PLoS ONE, 2012;7(5):e36800.
    Species: Rat
    Sample Type: Cell Lysates
    Application: IP
  16. Complement component C1q regulates macrophage expression of Mer tyrosine kinase to promote clearance of apoptotic cells.
    Authors: Galvan MD, Foreman DB, Zeng E
    J. Immunol., 2012;188(8):3716-23.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  17. Inhibiting Mer receptor tyrosine kinase suppresses STAT1, SOCS1/3, and NF-kappaB activation and enhances inflammatory responses in lipopolysaccharide-induced acute lung injury.
    J. Cell. Sci., 2012;91(6):921-32.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  18. Protein S controls hypoxic/ischemic blood-brain barrier disruption through the TAM receptor Tyro3 and sphingosine 1-phosphate receptor.
    Authors: Zhu D, Wang Y, Singh I
    Blood, 2010;115(23):4963-72.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  19. Protein S protects neurons from excitotoxic injury by activating the TAM receptor Tyro3-phosphatidylinositol 3-kinase-Akt pathway through its sex hormone-binding globulin-like region.
    Authors: Zhong Z, Wang Y, Guo H, Sagare A, Fernandez JA, Bell RD, Barrett TM, Griffin JH, Freeman RS, Zlokovic BV
    J. Neurosci., 2010;30(46):15521-34.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  20. Mertk receptor mutation reduces efferocytosis efficiency and promotes apoptotic cell accumulation and plaque necrosis in atherosclerotic lesions of apoe-/- mice.
    Authors: Thorp E, Cui D, Schrijvers DM, Kuriakose G, Tabas I
    Arterioscler. Thromb. Vasc. Biol., 2008;28(8):1421-8.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  21. A soluble form of the Mer receptor tyrosine kinase inhibits macrophage clearance of apoptotic cells and platelet aggregation.
    Authors: Sather S, Kenyon KD, Lefkowitz JB, Liang X, Varnum BC, Henson PM, Graham DK
    Blood, 2007;109(3):1026-33.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  22. Tetraspanin CD81 is required for the alpha v beta5-integrin-dependent particle-binding step of RPE phagocytosis.
    Authors: Chang Y, Finnemann SC
    J. Cell. Sci., 2007;120(0):3053-63.
    Species: Rat
    Sample Type: Cell Lysates
    Application: IP
  23. Gas6 receptors Axl, Sky and Mer enhance platelet activation and regulate thrombotic responses.
    Authors: Gould WR, Baxi SM, Schroeder R, Peng YW, Leadley RJ, Peterson JT, Perrin LA
    J. Thromb. Haemost., 2005;3(4):733-41.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
Expand to show all 23 Citations
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 application: Flow Cytometry.

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Summary

ApplicationFlow Cytometry
Sample TestedPeritoneal macrophages
SpeciesMouse

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