Mouse Mer Antibody

Catalog # Availability Size / Price Qty
AF591
AF591-SP
Mouse Mer Antibody in Flow Cytometry
3 Images
Product Details
Citations (29)
FAQs
Supplemental Products
Reviews (1)

Mouse Mer Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Mer in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross‑reactivity with recombinant human Mer is observed and less than 1% cross-reactivity with recombinant mouse (rm) Axl and rmDtk is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Mer
Glu23-Phe498
Accession # Q60805
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
See below
Immunohistochemistry
1-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
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1-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of Mer in J774A.1 Mouse Cell Line by Flow Cytometry. View Larger

Detection of Mer in J774A.1 Mouse Cell Line by Flow Cytometry.

J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Goat Anti-Mouse Mer Antigen Affinity-purified Polyclonal Antibody (Catalog # AF591, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).

Immunohistochemistry Mer in  Mouse Testis. View Larger

Mer in Mouse Testis. Mer was detected in perfusion fixed frozen sections of mouse testis using Goat Anti-Mouse Mer Antigen Affinity-purified Polyclonal Antibody (Catalog # AF591) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sperm cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Dual RNAscope ISH-IHC Compatible Mer in Mouse Kidney Using Dual RNAscope® ISH and IHC. View Larger

Mer in Mouse Kidney Using Dual RNAscope® ISH and IHC. Mer mRNA was detected in formalin-fixed paraffin-embedded tissue sections of mouse kidney probed with ACD RNAScope® Probe (Catalog # 441241) and stained using ACD RNAscope® 2.5 HD Detection Reagents-Red (top image, Catalog # 32260). Adjacent tissue section was processed for immunohistochemistry using R&D Systems Goat Anti-Mouse Mer Antigen Affinity-purified Polyclonal Antibody (Catalog # AF591) at 1 ug/mL with overnight incubation at 4 °C followed by incubation with the Anti-Goat IgG VisUCyte HRP Polymer Antibody (R&D Systems,  Catalog # VC004) and DAB chromogen (lower image, yellow-brown). Tissues were counterstained with hematoxylin (blue).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Mer

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt) and Mer (human and mouse homologues of chicken c-Eyk) constitute a receptor tyrosine kinase subfamily. The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. These receptors bind the vitamin K-dependent protein growth-arrest-specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration or the prevention of apoptosis. Studies suggest that this family of tyrosine kinase receptors may be involved in hematopoiesis, embryonic development, tumorigenesis and regulation of testicular functions (1, 2).

References
  1. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
  2. Crosier, K.E. and P.S Crosier (1997) Pathology 29:131.
Long Name
Receptor Tyrosine Protein Kinase Mer
Entrez Gene IDs
10461 (Human); 17289 (Mouse)
Alternate Names
c-Eyk; c-mer proto-oncogene tyrosine kinase; C-mer; EC 2.7.10; EC 2.7.10.1; MER receptor tyrosine kinase; Mer; MerTK; MGC133349; Receptor tyrosine kinase MerTK; RP38Proto-oncogene c-Mer; STK kinase; tyrosine-protein kinase Mer

Product Datasheets

Citations for Mouse Mer Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

29 Citations: Showing 1 - 10
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  1. Reference-based analysis of lung single-cell sequencing reveals a transitional profibrotic macrophage
    Authors: D Aran, AP Looney, L Liu, E Wu, V Fong, A Hsu, S Chak, RP Naikawadi, PJ Wolters, AR Abate, AJ Butte, M Bhattachar
    Nat. Immunol., 2019;20(2):163-172.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  2. Pan-TAM tyrosine kinase inhibitor BMS-777607 enhances anti-PD-1 mAb efficacy in a murine model of triple-negative breast cancer
    Authors: RB Birge, C Kasikara, V Davra, D Calianese, K Geng, TE Spires, M Quigley, M Wichroski, G Sriram, L Suarez-Lop, MB Yaffe, SV Kotenko, MS De Lorenzo
    Cancer Res., 2019;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Protective Role of the MER Tyrosine Kinase via Efferocytosis in Rheumatoid Arthritis Models
    Authors: CEJ Waterborg, S Beermann, MGA Broeren, MB Bennink, MI Koenders, PLEM van Lent, WB van den Be, PM van der Kr, FAJ van de Loo
    Front Immunol, 2018;9(0):742.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  4. Sitravatinib potentiates immune checkpoint blockade in refractory cancer models
    Authors: W Du, H Huang, N Sorrelle, RA Brekken
    JCI Insight, 2018;3(21):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  5. LXR Suppresses Inflammatory Gene Expression and Neutrophil Migration through cis-Repression and Cholesterol Efflux
    Authors: DG Thomas, AC Doran, P Fotakis, M Westerterp, P Antonson, H Jiang, XC Jiang, JÅ Gustafsson, I Tabas, AR Tall
    Cell Rep, 2018;25(13):3774-3785.e4.
  6. Macrophage deficiency of miR-21 promotes apoptosis, plaque necrosis, and vascular inflammation during atherogenesis
    Authors: A Canfrán-Du, N Rotllan, X Zhang, M Fernández-, C Ramírez-Hi, E Araldi, L Daimiel, R Busto, C Fernández-, Y Suárez
    EMBO Mol Med, 2017;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Migrating Interneurons Secrete Fractalkine to Promote Oligodendrocyte Formation in the Developing Mammalian Brain
    Authors: A Voronova, SA Yuzwa, BS Wang, S Zahr, C Syal, J Wang, DR Kaplan, FD Miller
    Neuron, 2017;94(3):500-516.e9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  8. Schwann cells use TAM receptor-mediated phagocytosis in addition to autophagy to clear myelin in a mouse model of nerve injury
    Authors: A Brosius Lu, WS Chung, SA Sloan, GA Carson, L Zhou, E Lovelett, S Posada, JB Zuchero, BA Barres
    Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  9. GAS6 is a key homeostatic immunological regulator of host-commensal interactions in the oral mucosa
    Authors: M Nassar, Y Tabib, T Capucha, G Mizraji, T Nir, M Pevsner-Fi, G Zilberman-, O Heyman, G Nussbaum, H Bercovier, A Wilensky, E Elinav, T Burstyn-Co, AH Hovav
    Proc. Natl. Acad. Sci. U.S.A, 2017;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  10. Sleep Loss Promotes Astrocytic Phagocytosis and Microglial Activation in Mouse Cerebral Cortex
    Authors: M Bellesi, L de Vivo, M Chini, F Gilli, G Tononi, C Cirelli
    J. Neurosci., 2017;37(21):5263-5273.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  11. MicroRNA-126 overexpression rescues diabetes-induced impairment in efferocytosis of apoptotic cardiomyocytes
    Sci Rep, 2016;6(0):36207.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  12. TAM receptors regulate multiple features of microglial physiology
    Authors: L Fourgeaud, PG Través, Y Tufail, H Leal-Baile, ED Lew, PG Burrola, P Callaway, A Zagórska, CV Rothlin, A Nimmerjahn, G Lemke
    Nature, 2016;532(7598):240-4.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  13. Liver X receptor and STAT1 cooperate downstream of Gas6/Mer to induce anti-inflammatory arginase 2 expression in macrophages
    Sci Rep, 2016;6(0):29673.
    Species: Mouse
    Sample Types: In vitro
    Applications: in vitro
  14. MerTK cleavage limits proresolving mediator biosynthesis and exacerbates tissue inflammation
    Proc Natl Acad Sci USA, 2016;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  15. Collecting lymphatic vessel permeability facilitates adipose tissue inflammation and distribution of antigen to lymph node-homing adipose tissue dendritic cells.
    Authors: Kuan E, Ivanov S, Bridenbaugh E, Victora G, Wang W, Childs E, Platt A, Jakubzick C, Mason R, Gashev A, Nussenzweig M, Swartz M, Dustin M, Zawieja D, Randolph G
    J Immunol, 2015;194(11):5200-10.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. Apolipoprotein E promotes subretinal mononuclear phagocyte survival and chronic inflammation in age-related macular degeneration.
    Authors: Levy O, Calippe B, Lavalette S, Hu S, Raoul W, Dominguez E, Housset M, Paques M, Sahel J, Bemelmans A, Combadiere C, Guillonneau X, Sennlaub F
    EMBO Mol Med, 2015;7(2):211-26.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  17. A new mouse model for female genital schistosomiasis.
    Authors: Richardson, Monica L, Fu, Chi-Ling, Pennington, Luke F, Honeycutt, Jared D, Odegaard, Justin I, Hsieh, Yi-Ju, Hammam, Olfat, Conti, Simon L, Hsieh, Michael
    PLoS Negl Trop Dis, 2014;8(5):e2825.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Flow Cytometry
  18. Diversification of TAM receptor tyrosine kinase function.
    Authors: Zagorska A, Traves P, Lew E, Dransfield I, Lemke G
    Nat Immunol, 2014;15(10):920-8.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  19. Ly6Chi monocyte recruitment is responsible for Th2 associated host-protective macrophage accumulation in liver inflammation due to schistosomiasis.
    Authors: Nascimento M, Huang S, Smith A, Everts B, Lam W, Bassity E, Gautier E, Randolph G, Pearce E
    PLoS Pathog, 2014;10(8):e1004282.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  20. Minimal differentiation of classical monocytes as they survey steady-state tissues and transport antigen to lymph nodes.
    Authors: Jakubzick C, Gautier E, Gibbings S, Sojka D, Schlitzer A, Johnson T, Ivanov S, Duan Q, Bala S, Condon T, Van Rooijen N, Grainger J, Belkaid Y, Ma'ayan A, Riches D, Yokoyama W, Ginhoux F, Henson P, Randolph G
    Immunity, 2013;39(3):599-610.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  21. Complement component C1q regulates macrophage expression of Mer tyrosine kinase to promote clearance of apoptotic cells.
    Authors: Galvan MD, Foreman DB, Zeng E
    J. Immunol., 2012;188(8):3716-23.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  22. Cross-phosphorylation, signaling and proliferative functions of the Tyro3 and Axl receptors in Rat2 cells.
    Authors: Brown JE, Krodel M, Pazos M
    PLoS ONE, 2012;7(5):e36800.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  23. Inhibiting Mer receptor tyrosine kinase suppresses STAT1, SOCS1/3, and NF-kappaB activation and enhances inflammatory responses in lipopolysaccharide-induced acute lung injury.
    J. Cell. Sci., 2012;91(6):921-32.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  24. Protein S controls hypoxic/ischemic blood-brain barrier disruption through the TAM receptor Tyro3 and sphingosine 1-phosphate receptor.
    Authors: Zhu D, Wang Y, Singh I
    Blood, 2010;115(23):4963-72.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  25. Protein S protects neurons from excitotoxic injury by activating the TAM receptor Tyro3-phosphatidylinositol 3-kinase-Akt pathway through its sex hormone-binding globulin-like region.
    Authors: Zhong Z, Wang Y, Guo H, Sagare A, Fernandez JA, Bell RD, Barrett TM, Griffin JH, Freeman RS, Zlokovic BV
    J. Neurosci., 2010;30(46):15521-34.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  26. Mertk receptor mutation reduces efferocytosis efficiency and promotes apoptotic cell accumulation and plaque necrosis in atherosclerotic lesions of apoe-/- mice.
    Authors: Thorp E, Cui D, Schrijvers DM, Kuriakose G, Tabas I
    Arterioscler. Thromb. Vasc. Biol., 2008;28(8):1421-8.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  27. A soluble form of the Mer receptor tyrosine kinase inhibits macrophage clearance of apoptotic cells and platelet aggregation.
    Authors: Sather S, Kenyon KD, Lefkowitz JB, Liang X, Varnum BC, Henson PM, Graham DK
    Blood, 2007;109(3):1026-33.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  28. Tetraspanin CD81 is required for the alpha v beta5-integrin-dependent particle-binding step of RPE phagocytosis.
    Authors: Chang Y, Finnemann SC
    J. Cell. Sci., 2007;120(0):3053-63.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  29. Gas6 receptors Axl, Sky and Mer enhance platelet activation and regulate thrombotic responses.
    Authors: Gould WR, Baxi SM, Schroeder R, Peng YW, Leadley RJ, Peterson JT, Perrin LA
    J. Thromb. Haemost., 2005;3(4):733-41.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Mouse Mer Antibody
By Anonymous on 06/17/2017
Application: Flow Sample Tested: Peritoneal macrophages Species: Mouse