Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse, Rat, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Flow Cytometry, Dual RNAscope ISH-IHC Compatible, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Neutralization, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation, Activation, Cell Culture, ELISA Capture, Proximity Ligation Assay, In vivo assay, Functional Assay, IF/IHC, CyTOF-reported

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Mer Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Mer
Glu23-Phe498
Accession # Q60805

Specificity

Detects mouse Mer in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross‑reactivity with recombinant human Mer is observed and less than 1% cross-reactivity with recombinant mouse (rm) Axl and rmDtk is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse Mer Antibody

Detection of Mer antibody in J774A.1 Mouse Cell Line antibody by Flow Cytometry.

Detection of Mer in J774A.1 Mouse Cell Line by Flow Cytometry.

J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Goat Anti-Mouse Mer Antigen Affinity-purified Polyclonal Antibody (Catalog # AF591, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).

Mer antibody in Mouse Testis by Immunohistochemistry (IHC-Fr).

Mer in Mouse Testis.

Mer was detected in perfusion fixed frozen sections of mouse testis using Goat Anti-Mouse Mer Antigen Affinity-purified Polyclonal Antibody (Catalog # AF591) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sperm cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Mer antibody in Mouse Kidney Using Dual RNAscope®ISH and IHC.

Mer in Mouse Kidney Using Dual RNAscope®ISH and IHC.

Mer mRNA was detected in formalin-fixed paraffin-embedded tissue sections of mouse kidney probed with ACD RNAScope®Probe (Catalog # 441241) and stained using ACD RNAscope®2.5 HD Detection Reagents-Red (top image, Catalog # 32260). Adjacent tissue section was processed for immunohistochemistry using R&D Systems Goat Anti-Mouse Mer Antigen Affinity-purified Polyclonal Antibody (Catalog # AF591) at 1 ug/mL with overnight incubation at 4 °C followed by incubation with the Anti-Goat IgG VisUCyte HRP Polymer Antibody (R&D Systems, Catalog # VC004) and DAB chromogen (lower image, yellow-brown). Tissues were counterstained with hematoxylin (blue).

Detection of Mouse Mer by Western Blot

Detection of Mouse Mer by Western Blot

RPE express components of BAI1-signaling pathway and Bai1Tg. (A) Expression of the BAI1 signaling pathway was analyzed by quantitative RT-PCR in RPE isolated from P14 Mertk+/− and Mertk−/− mice (n = 2) Error bars represent SEM. (B) Immunoblot analysis of BAI1 signaling pathway in RPE whole cell lysates isolated from P14 mice. Left panel shows representative immunoblots for Mer, Dock180, Elmo2 and Rac. Right panel shows combined densitometry analysis of immunoblots from n = 3 mice per genotype. For densitometry analysis, band volumes were normalized to an actin loading control and band densities in Mertk−/− were then normalized to Mertk+/− for comparison across multiple blots. (C) Schematic of Bai1Tg insertion in the Rosa26 locus indicating the N-terminal HA-tag in red and IRES-GFP in green. Mouse image licensed from Motifolio Inc. (D) GFP expression was analyzed in RPE flat mount preparations at 20x magnification. Inset image in the left panel shows a representative flat mount at 1.2x magnification. White scale bars in images are 50 μm. Images are representative of n = 2 mice per genotype. (E) qRT-PCR analysis of Bai1Tg expression in Mertk+/−Bai1Tg (n = 9) and Mertk−/−Bai1Tg (n = 3) RPE isolated on P14. n.d. = not detected. Error bars represent SEM. (F) Immunoblot analysis of HA-tag (Bai1Tg) in RPE whole cell lysates isolated from P13-P14 mice. Immunoblot shown is representative of n = 2 experiments. (G) HA staining and localization was analyzed in eyecups at 40x magnification. Neural retinas were removed prior to fixation and staining. Black lines next to the figure align with apical microvilli of RPE. Scale bars are 20 μm. Images shown are representative of n = 2 mice per genotype. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41598-017-15191-1), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse Mer Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Dual RNAscope ISH-IHC Compatible

1-15 µg/mL
Sample: Immersion fixed paraffin-embedded tissue sections of mouse kidney

Flow Cytometry

2.5 µg/106 cells
Sample:
J774A.1 mouse reticulum cell sarcoma macrophage cell line

Immunohistochemistry

1-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse testis

Reviewed Applications

Read 4 reviews rated 4.5 using AF591 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Mer

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt) and Mer (human and mouse homologues of chicken c-Eyk) constitute a receptor tyrosine kinase subfamily. The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. These receptors bind the vitamin K-dependent protein growth-arrest-specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration or the prevention of apoptosis. Studies suggest that this family of tyrosine kinase receptors may be involved in hematopoiesis, embryonic development, tumorigenesis and regulation of testicular functions (1, 2).

References

  1. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
  2. Crosier, K.E. and P.S Crosier (1997) Pathology 29:131.

Long Name

Receptor Tyrosine Protein Kinase Mer

Alternate Names

c-Eyk, C-mer, MerTK

Entrez Gene IDs

10461 (Human); 17289 (Mouse); 102143798 (Cynomolgus Monkey)

Gene Symbol

MERTK

UniProt

Q60805

Additional Mer Products

Product Documents for Mouse Mer Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Mer Antibody

For research use only

Citations for Mouse Mer Antibody

Customer Reviews for Mouse Mer Antibody (4)

4.5 out of 5
4 Customer Ratings
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Customer Images

Showing  1 - 4 of 4 reviews Showing All
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  • Mouse Mer Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: lysates of primary bone marrow derived macrophage
    Species: mice
    Verified Customer | Posted 11/08/2022
    The band comes around 170Kda
    Mouse Mer Antibody AF591
  • Mouse Mer Antibody
    Name: Fuyi Chen
    Application: immunohistochemistry free floating section
    Sample Tested: Adult mouse brain
    Species: Mouse
    Verified Customer | Posted 03/16/2018
    Mer is in green and Iba1 is in red.
    antigen retrieval for 15 mins. 1:250 dilution.
    Mouse Mer Antibody AF591
  • Mouse Mer Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Peritoneal macrophages
    Species: Mouse
    Verified Customer | Posted 06/17/2017
  • Mouse Mer Antibody
    Name: Anonymous
    Application: in vivo
    Sample Tested: Adult mouse brain and brain and spinal cord
    Species: Mouse
    Verified Customer | Posted 04/03/2017
    Used in vivo to inhibit Mer activation.

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Protocols

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