Mouse Mer Antibody

Name: Mouse Mer Antibody
Brand: R&D Systems
SKU: AF591-SP
Rating: 5 (1 reviews)

AF591 30 Citations 1 Review Datasheet / COA / SDS

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AF591-SP

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Citations (30)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Mer Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Mer in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross‑reactivity with recombinant human Mer is observed and less than 1% cross-reactivity with recombinant mouse (rm) Axl and rmDtk is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Mer
Glu23-Phe498
Accession # Q60805

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

See below

Immunohistochemistry

1-15 µg/mL

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Dual RNAscope ISH-IHC

1-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry

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Detection of Mer in J774A.1 Mouse Cell Line by Flow Cytometry. J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Goat Anti-Mouse Mer Antigen Affinity-purified Polyclonal Antibody (Catalog # AF591, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).

Immunohistochemistry

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Mer in Mouse Testis. Mer was detected in perfusion fixed frozen sections of mouse testis using Goat Anti-Mouse Mer Antigen Affinity-purified Polyclonal Antibody (Catalog # AF591) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sperm cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Dual RNAscope ISH-IHC Compatible

Mer in Mouse Kidney Using Dual RNAscope<sup>®</sup>ISH and IHC.

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Mer in Mouse Kidney Using Dual RNAscope^®ISH and IHC. Mer mRNA was detected in formalin-fixed paraffin-embedded tissue sections of mouse kidney probed with ACD RNAScope^®Probe (Catalog # 441241) and stained using ACD RNAscope^®2.5 HD Detection Reagents-Red (top image, Catalog # 32260). Adjacent tissue section was processed for immunohistochemistry using R&D Systems Goat Anti-Mouse Mer Antigen Affinity-purified Polyclonal Antibody (Catalog # AF591) at 1 ug/mL with overnight incubation at 4 °C followed by incubation with the Anti-Goat IgG VisUCyte HRP Polymer Antibody (R&D Systems, Catalog # VC004) and DAB chromogen (lower image, yellow-brown). Tissues were counterstained with hematoxylin (blue).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Mer

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt) and Mer (human and mouse homologues of chicken c-Eyk) constitute a receptor tyrosine kinase subfamily. The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. These receptors bind the vitamin K-dependent protein growth-arrest-specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration or the prevention of apoptosis. Studies suggest that this family of tyrosine kinase receptors may be involved in hematopoiesis, embryonic development, tumorigenesis and regulation of testicular functions (1, 2).

References

Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
Crosier, K.E. and P.S Crosier (1997) Pathology 29:131.

Long Name

Receptor Tyrosine Protein Kinase Mer

Entrez Gene IDs

10461 (Human); 17289 (Mouse); 102143798 (Cynomolgus Monkey)

Alternate Names

c-Eyk; c-mer proto-oncogene tyrosine kinase; C-mer; EC 2.7.10; EC 2.7.10.1; MER receptor tyrosine kinase; Mer; MerTK; MGC133349; Receptor tyrosine kinase MerTK; RP38Proto-oncogene c-Mer; STK kinase; tyrosine-protein kinase Mer

Product Datasheets

Product Datasheet

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Related Research Areas

Citations for Mouse Mer Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

30 Citations: Showing 1 - 10
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A FUNCTIONAL ROLE OF GAS6/TAM IN NON-ALCOHOLIC STEATOHEPATITIS PROGRESSION IMPLICATES AXL AS THERAPEUTIC TARGET
Authors: A Tutusaus, E de Gregori, B Cucarull, H Cristóbal, C Aresté, I Graupera, M Coll, A Colell, G Gausdal, JB Lorens, P García de, A Morales, M Marí
Cell Mol Gastroenterol Hepatol, 2019;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture
Reference-based analysis of lung single-cell sequencing reveals a transitional profibrotic macrophage
Authors: D Aran, AP Looney, L Liu, E Wu, V Fong, A Hsu, S Chak, RP Naikawadi, PJ Wolters, AR Abate, AJ Butte, M Bhattachar
Nat. Immunol., 2019;20(2):163-172.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr
Pan-TAM tyrosine kinase inhibitor BMS-777607 enhances anti-PD-1 mAb efficacy in a murine model of triple-negative breast cancer
Authors: RB Birge, C Kasikara, V Davra, D Calianese, K Geng, TE Spires, M Quigley, M Wichroski, G Sriram, L Suarez-Lop, MB Yaffe, SV Kotenko, MS De Lorenzo
Cancer Res., 2019;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
Protective Role of the MER Tyrosine Kinase via Efferocytosis in Rheumatoid Arthritis Models
Authors: CEJ Waterborg, S Beermann, MGA Broeren, MB Bennink, MI Koenders, PLEM van Lent, WB van den Be, PM van der Kr, FAJ van de Loo
Front Immunol, 2018;9(0):742.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo
Sitravatinib potentiates immune checkpoint blockade in refractory cancer models
Authors: W Du, H Huang, N Sorrelle, RA Brekken
JCI Insight, 2018;3(21):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
LXR Suppresses Inflammatory Gene Expression and Neutrophil Migration through cis-Repression and Cholesterol Efflux
Authors: DG Thomas, AC Doran, P Fotakis, M Westerterp, P Antonson, H Jiang, XC Jiang, JÅ Gustafsson, I Tabas, AR Tall
Cell Rep, 2018;25(13):3774-3785.e4.
GAS6 is a key homeostatic immunological regulator of host-commensal interactions in the oral mucosa
Authors: M Nassar, Y Tabib, T Capucha, G Mizraji, T Nir, M Pevsner-Fi, G Zilberman-, O Heyman, G Nussbaum, H Bercovier, A Wilensky, E Elinav, T Burstyn-Co, AH Hovav
Proc. Natl. Acad. Sci. U.S.A, 2017;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
Migrating Interneurons Secrete Fractalkine to Promote Oligodendrocyte Formation in the Developing Mammalian Brain
Authors: A Voronova, SA Yuzwa, BS Wang, S Zahr, C Syal, J Wang, DR Kaplan, FD Miller
Neuron, 2017;94(3):500-516.e9.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization
Schwann cells use TAM receptor-mediated phagocytosis in addition to autophagy to clear myelin in a mouse model of nerve injury
Authors: A Brosius Lu, WS Chung, SA Sloan, GA Carson, L Zhou, E Lovelett, S Posada, JB Zuchero, BA Barres
Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
Macrophage deficiency of miR-21 promotes apoptosis, plaque necrosis, and vascular inflammation during atherogenesis
Authors: A Canfrán-Du, N Rotllan, X Zhang, M Fernández-, C Ramírez-Hi, E Araldi, L Daimiel, R Busto, C Fernández-, Y Suárez
EMBO Mol Med, 2017;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Sleep Loss Promotes Astrocytic Phagocytosis and Microglial Activation in Mouse Cerebral Cortex
Authors: M Bellesi, L de Vivo, M Chini, F Gilli, G Tononi, C Cirelli
J. Neurosci., 2017;37(21):5263-5273.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

TAM receptors regulate multiple features of microglial physiology
Authors: L Fourgeaud, PG Través, Y Tufail, H Leal-Baile, ED Lew, PG Burrola, P Callaway, A Zagórska, CV Rothlin, A Nimmerjahn, G Lemke
Nature, 2016;532(7598):240-4.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

Liver X receptor and STAT1 cooperate downstream of Gas6/Mer to induce anti-inflammatory arginase 2 expression in macrophages
Sci Rep, 2016;6(0):29673.
Species: Mouse
Sample Types: In vitro
Applications: in vitro

MicroRNA-126 overexpression rescues diabetes-induced impairment in efferocytosis of apoptotic cardiomyocytes
Sci Rep, 2016;6(0):36207.
Species: Mouse
Sample Types: Cell Lysates
Applications: Immunoprecipitation

MerTK cleavage limits proresolving mediator biosynthesis and exacerbates tissue inflammation
Proc Natl Acad Sci USA, 2016;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Apolipoprotein E promotes subretinal mononuclear phagocyte survival and chronic inflammation in age-related macular degeneration.
Authors: Levy O, Calippe B, Lavalette S, Hu S, Raoul W, Dominguez E, Housset M, Paques M, Sahel J, Bemelmans A, Combadiere C, Guillonneau X, Sennlaub F
EMBO Mol Med, 2015;7(2):211-26.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Collecting lymphatic vessel permeability facilitates adipose tissue inflammation and distribution of antigen to lymph node-homing adipose tissue dendritic cells.
Authors: Kuan E, Ivanov S, Bridenbaugh E, Victora G, Wang W, Childs E, Platt A, Jakubzick C, Mason R, Gashev A, Nussenzweig M, Swartz M, Dustin M, Zawieja D, Randolph G
J Immunol, 2015;194(11):5200-10.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

Ly6Chi monocyte recruitment is responsible for Th2 associated host-protective macrophage accumulation in liver inflammation due to schistosomiasis.
Authors: Nascimento M, Huang S, Smith A, Everts B, Lam W, Bassity E, Gautier E, Randolph G, Pearce E
PLoS Pathog, 2014;10(8):e1004282.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

A new mouse model for female genital schistosomiasis.
Authors: Richardson, Monica L, Fu, Chi-Ling, Pennington, Luke F, Honeycutt, Jared D, Odegaard, Justin I, Hsieh, Yi-Ju, Hammam, Olfat, Conti, Simon L, Hsieh, Michael
PLoS Negl Trop Dis, 2014;8(5):e2825.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Flow Cytometry

Diversification of TAM receptor tyrosine kinase function.
Authors: Zagorska A, Traves P, Lew E, Dransfield I, Lemke G
Nat Immunol, 2014;15(10):920-8.
Species: Mouse
Sample Types: Cell Lysates
Applications: Immunoprecipitation

Minimal differentiation of classical monocytes as they survey steady-state tissues and transport antigen to lymph nodes.
Authors: Jakubzick C, Gautier E, Gibbings S, Sojka D, Schlitzer A, Johnson T, Ivanov S, Duan Q, Bala S, Condon T, Van Rooijen N, Grainger J, Belkaid Y, Ma'ayan A, Riches D, Yokoyama W, Ginhoux F, Henson P, Randolph G
Immunity, 2013;39(3):599-610.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

Complement component C1q regulates macrophage expression of Mer tyrosine kinase to promote clearance of apoptotic cells.
Authors: Galvan MD, Foreman DB, Zeng E
J. Immunol., 2012;188(8):3716-23.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Inhibiting Mer receptor tyrosine kinase suppresses STAT1, SOCS1/3, and NF-kappaB activation and enhances inflammatory responses in lipopolysaccharide-induced acute lung injury.
J. Cell. Sci., 2012;91(6):921-32.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization

Cross-phosphorylation, signaling and proliferative functions of the Tyro3 and Axl receptors in Rat2 cells.
Authors: Brown JE, Krodel M, Pazos M
PLoS ONE, 2012;7(5):e36800.
Species: Rat
Sample Types: Cell Lysates
Applications: Immunoprecipitation

Protein S controls hypoxic/ischemic blood-brain barrier disruption through the TAM receptor Tyro3 and sphingosine 1-phosphate receptor.
Authors: Zhu D, Wang Y, Singh I
Blood, 2010;115(23):4963-72.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Protein S protects neurons from excitotoxic injury by activating the TAM receptor Tyro3-phosphatidylinositol 3-kinase-Akt pathway through its sex hormone-binding globulin-like region.
Authors: Zhong Z, Wang Y, Guo H, Sagare A, Fernandez JA, Bell RD, Barrett TM, Griffin JH, Freeman RS, Zlokovic BV
J. Neurosci., 2010;30(46):15521-34.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Mertk receptor mutation reduces efferocytosis efficiency and promotes apoptotic cell accumulation and plaque necrosis in atherosclerotic lesions of apoe-/- mice.
Authors: Thorp E, Cui D, Schrijvers DM, Kuriakose G, Tabas I
Arterioscler. Thromb. Vasc. Biol., 2008;28(8):1421-8.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P

Tetraspanin CD81 is required for the alpha v beta5-integrin-dependent particle-binding step of RPE phagocytosis.
Authors: Chang Y, Finnemann SC
J. Cell. Sci., 2007;120(0):3053-63.
Species: Rat
Sample Types: Cell Lysates
Applications: Immunoprecipitation

A soluble form of the Mer receptor tyrosine kinase inhibits macrophage clearance of apoptotic cells and platelet aggregation.
Authors: Sather S, Kenyon KD, Lefkowitz JB, Liang X, Varnum BC, Henson PM, Graham DK
Blood, 2007;109(3):1026-33.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Gas6 receptors Axl, Sky and Mer enhance platelet activation and regulate thrombotic responses.
Authors: Gould WR, Baxi SM, Schroeder R, Peng YW, Leadley RJ, Peterson JT, Perrin LA
J. Thromb. Haemost., 2005;3(4):733-41.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry