Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Electron Microscopy

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all MFRP Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse MFRP
Gln91-Pro584
Accession # Q8K480

Specificity

Detects mouse MFRP in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 30% cross-reactivity with recombinant human (rh) MFRP is observed, 15% cross-reactivity with recombinant mouse (rm) Frizzled-4 is observed, 10% cross-reactivity with rmFrizzled-1, rhFrizzled-5, rmFrizzled-8, and rmFrizzled-10 is observed, and 5% cross-reactivity with rmFrizzled-3, rmFrizzled-6, and rmFrizzled-7 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse MFRP Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse brain (cerebellum and medulla)

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse MFRP

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MFRP

MFRP is a type II transmembrane protein that is related to a small family of soluble frizzled-related proteins. It is the product of a dicistronic mRNA transcript that codes for two proteins; MFRP and C1qTNF5. Mouse MFRP is 584 amino acids (aa) in length. Its extracellular domain (ECD) of 494 aa contains two CUB and two LDLR class A segments, plus one frizzled domain. At least two splice variants are known in mouse. In isoform 2, amino acid residues 425‑431 of isoform 1 is substituted with Lys. Mouse MFRP ECD shares 72% aa identity with human MFRP ECD. In mouse retinal degeneration 6, MFRP is mutated.

Long Name

Membrane Frizzled-related Protein

Alternate Names

MCOP5, membrane frizzled-related protein, Membrane-type frizzled-related protein, MGC32938, NNO2, RD6

Entrez Gene IDs

83552 (Human); 259172 (Mouse)

Gene Symbol

MFRP

UniProt

Q8K480

Additional MFRP Products

Product Documents for Mouse MFRP Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse MFRP Antibody

For research use only

Related Research Areas

Wnt Family
Wnt Signaling Modulators

Citations for Mouse MFRP Antibody

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Protocols

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