Mouse MFRP Antibody

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Product Details
Citations (2)
Supplemental Products

Mouse MFRP Antibody Summary

Species Reactivity
Detects mouse MFRP in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 30% cross-reactivity with recombinant human (rh) MFRP is observed, 15% cross-reactivity with recombinant mouse (rm) Frizzled-4 is observed, 10% cross-reactivity with rmFrizzled-1, rhFrizzled-5, rmFrizzled-8, and rmFrizzled-10 is observed, and 5% cross-reactivity with rmFrizzled-3, rmFrizzled-6, and rmFrizzled-7 is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant mouse MFRP
Accession # Q8K480
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Mouse MFRP
5-15 µg/mL
Perfusion fixed frozen sections of mouse brain (cerebellum and medulla)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MFRP

MFRP is a type II transmembrane protein that is related to a small family of soluble frizzled-related proteins. It is the product of a dicistronic mRNA transcript that codes for two proteins; MFRP and C1qTNF5. Mouse MFRP is 584 amino acids (aa) in length. Its extracellular domain (ECD) of 494 aa contains two CUB and two LDLR class A segments, plus one frizzled domain. At least two splice variants are known in mouse. In isoform 2, amino acid residues 425‑431 of isoform 1 is substituted with Lys. Mouse MFRP ECD shares 72% aa identity with human MFRP ECD. In mouse retinal degeneration 6, MFRP is mutated.

Long Name
Membrane Frizzled-related Protein
Entrez Gene IDs
83552 (Human); 259172 (Mouse)
Alternate Names
MCOP5; membrane frizzled-related protein; Membrane-type frizzled-related protein; MFRP; MGC32938; NNO2; rd6

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Citations for Mouse MFRP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. ADIPOR1 is essential for vision and its RPE expression is lost in the Mfrprd6 mouse
    Authors: VM Sluch, A Banks, H Li, MA Crowley, V Davis, C Xiang, J Yang, JT Demirs, J Vrouvliani, B Leehy, S Hanks, AM Hyman, J Aranda, B Chang, CE Bigelow, DS Rice
    Sci Rep, 2018-09-25;8(1):14339.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Spatial and temporal expression of MFRP and its interaction with CTRP5.
    Authors: Mandal MN, Vasireddy V, Jablonski MM
    Invest. Ophthalmol. Vis. Sci., 2006-12-01;47(12):5514-21.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: Electron Microscopy, IHC-Fr, Western Blot


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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody


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