Mouse MUP-1 Antibody

Catalog # Availability Size / Price Qty
MAB6560
MAB6560-SP
MUP‑1 in NCTC 1469 Mouse Cell Line.
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Mouse MUP-1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse MUP-1 in direct ELISAs.
Source
Monoclonal Mouse IgM Clone # 663323
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse MUP-1 synthetic peptide
Accession # NP_112465
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry MUP-1 antibody in NCTC 1469 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

MUP‑1 in NCTC 1469 Mouse Cell Line. MUP-1 was detected in immersion fixed NCTC 1469 mouse liver cell line using Mouse MUP-1 Monoclonal Antibody (Catalog # MAB6560) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI. Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MUP-1

Major urinary protein 1 (MUP-1) is a 20 kDa lipocalin family glycoprotein that is secreted by the liver into the circulation and then excreted in the urine of male mice. Its beta -barrel structure with an internal hydrophobic cavity enables MUP-1 to serve as a carrier for a variety of pheromones. In the circulation, MUP-1 promotes energy expenditure, mitochondrial biogenesis, and oxidative function, and it inhibits hepatic glucose production, triglyceride accumulation, glucose intolerance, and insulin resistance. MUP-1 is downregulated in the liver and circulation of diabetic obese mice.

Long Name
Major Urinary Protein 1
Entrez Gene IDs
17840 (Mouse)
Alternate Names
Ltn-1; Lvtn-1; major urinary protein 1; major urinary protein 10; major urinary protein 7; MUP 1; Mup1 major urinary protein 1; MUP1; MUP-1; Mup10; Mup7; Mup-a; Up-1

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Reconstitution Buffers

Secondary Antibodies

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