Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Agonist Activity

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Neutralization, Flow Cytometry, Functional Assay

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all RANK/TNFRSF11A Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse RANK/TNFRSF11A
Gln30-Pro213
Accession # O35305

Specificity

Detects mouse RANK/TNFRSF11A in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human RANK is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse RANK/TNFRSF11A Antibody

RANK/TNFRSF11A antibody in Rat Embryo by Immunohistochemistry (IHC-Fr).

RANK/TNFRSF11A in Rat Embryo.

RANK/TNFRSF11A was detected in immersion fixed frozen sections of rat embryo (cartillage primordium, 7 days p.c.) using 10 µg/mL Goat Anti-Mouse RANK/TNFRSF11A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF692) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Mouse RANK/TNFRSF11A Antibody Agonist Activity.

Mouse RANK/TNFRSF11A Antibody (Catalog # AF692) induces osteoclast differentiation of RAW 264.7 mouse monocyte/macrophage cells in the presence of recombinant mouse M-CSF (416ML). The ED50 for this effect is typically 0.0200 – 0.400 μg/mL.
Detection of RANK/TNFRSF11A by Immunohistochemistry

Detection of RANK/TNFRSF11A by Immunohistochemistry

RANK loss in tumor cells leads to increased CD8+ Tcell tumor infiltration that mediates the delayed tumor latency of RANK−/− tumors. Representative images (b) of CD3+ (n = 4 tumors, ***p = 0.0009) and CD8+ cells (n = 6 tumors, ***p = 0.0001) in RANK+/+ and RANK−/− tumor transplants as assessed by IHC. Scale = 25 μm. Tumors derived from three independent primary tumors were used. Each dot represents one picture#. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33303745), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse RANK/TNFRSF11A by Immunohistochemistry

Detection of Mouse RANK/TNFRSF11A by Immunohistochemistry

RANKL, RANK, OPG and LGR4 expression patterns in the mandible first molar of 5-day-old wild-type mice. (A) Immunohistochemistry experiments were carried out on 5-µm-thick frontal sections of 5-day-old wild-type C57BL/6 mouse heads for RANKL, RANK, OPG and LGR4. RANKL and OPG expressions were observed in some mesenchymal cells in the pulp, mainly facing Hertwig’s epithelial root sheath, in mesenchymal cells in the apical papilla, and in certain alveolar bone cells (arrowheads). RANK expression was high in the pulp, in Hertwig’s epithelial root sheath and in the cells at the bone surface. Mild LGR4 expression was evidenced in some cells in Hertwig’s epithelial root sheath and the apical papilla (arrowheads), and in most cells at the alveolar bone surface. Scale: 20X/100 µm. (B) Schematic representations of established RANKL, RANK, OPG and LGR4 expression patterns in 5-day-old wild-type C57BL/6 mice mandibular first molar roots. The cells in Hertwig’s epithelial root sheath expressed only RANK and LGR4, with a significant difference in the number of stained cells and the staining intensity in favor of RANK. DE: dental epithelium; E: enamel; D: dentin; P: pulp; HERS: Hertwig’s epithelial root sheath. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32209985), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse RANK/TNFRSF11A Antibody

Application
Recommended Usage

Agonist Activity

Mouse RANK/TNFRSF11A Antibody (Catalog # AF692) induces osteoclast differentiation of RAW 264.7 mouse monocyte/macrophage cells in the presence of recombinant mouse M-CSF (Catalog # 416ML). The ED50 for this effect is typically 0.0200 – 0.400 μg/mL.

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed frozen sections of rat embryo (cartillage primordium, 7 d.p.c.) subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse RANK/TNFRSF11A Fc Chimera (Catalog # 692-RK)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: RANK/TNFRSF11A

RANK (receptor activator of NF-kappa B, also known as TRANCE receptor, osteoclast differentiation factor receptor [ODFR] and TNFRSF11A) is a member of the tumor necrosis factor receptor family. The full length mouse RANK cDNA encodes a type I transmembrane protein of 625 amino acids (aa) with a predicted 184 aa extracellular domain and a 391 aa cytoplasmic domain. The extracellular domain contains two potential N-linked glycosylation sites. RANK shares significant amino acid homology with other members of the TNF R family in its extracellular four cysteine-rich repeats. Human and murine RANK share 81% aa identity in their extracellular domains. RANK is widely expressed with the highest levels in skeletal muscle, thymus, liver, colon, small intestine and adrenal gland. RANK is expressed in dendritic cells. In activated human peripheral blood T lymphocytes, RANK expression is induced by IL-4 and TGF-beta. Multiple tumor necrosis factor receptor-associated factors (TRAFs) are involved in the signaling of RANK. TRANCE (TNF-related activation-induced cytokines, also known as RANK ligand [RANKL], osteoprotegerin ligand [OPGL], and osteoclast differentiation factor [ODF]) is the ligand for RANK. The biological functions mediated through RANK include activation of NF-kappa B and c-jun N-terminal kinase, enhancement of T cell growth and dendritic cell function, induction of osteoclastogenesis, and lymph node organogenesis. Soluble RANK is able to block TRANCE induced biological activity.

References

  1. Anderson, D.M. et al. (1997) Nature 390:175.
  2. Nakagawa, N. et al. (1998) Biochem. Biophys. Res. Commun. 245:382.

Long Name

Receptor Activator of NF-kappa-B

Alternate Names

CD265, ODFR, TNFRSF11A, TRANCE R

Entrez Gene IDs

8792 (Human); 21934 (Mouse)

Gene Symbol

TNFRSF11A

UniProt

O35305

Additional RANK/TNFRSF11A Products

Product Documents for Mouse RANK/TNFRSF11A Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse RANK/TNFRSF11A Antibody

For research use only

Citations for Mouse RANK/TNFRSF11A Antibody

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Protocols

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