Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all RARRES1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse RARRES1
Pro42-Phe283
Accession # F6ZIS7

Specificity

Detects mouse RARRES1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human RARRES1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse RARRES1 Antibody

Detection of Mouse RARRES1 antibody by Western Blot.

Detection of Mouse RARRES1 by Western Blot.

Western blot shows lysates of mouse spleen tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse RARRES1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4657) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for RARRES1 at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Mouse RARRES1 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse liver

Western Blot

1 µg/mL
Sample: Mouse spleen tissue

Reviewed Applications

Read 1 review rated 5 using AF4657 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: RARRES1

RARRES1 (retinoic acid receptor responder 1; also Tig1) is a member of the protease inhibitor I47 family of proteins. It is expressed in epithelium, including keratinocytes, plus prostate and breast epithelium. It is considered to be a tumor-suppressor molecule, presumably acting as an inhibitor of carboxypeptidase A. Mouse RARRES1 is 283 amino acids (aa) in length. Based on human, it is a type II transmembrane (TM) protein with a TM segment at, or near, aa 75‑95. Over
aa 42-283, mouse RARRES1 shares 85% and 74% aa identity with rat and human RARRES1, respectively.

Long Name

Retinoic Acid Receptor Responder [Tazarotene-induced] 1

Alternate Names

TIG1

Entrez Gene IDs

5918 (Human); 109222 (Mouse); 310486 (Rat)

Gene Symbol

RARRES1

UniProt

F6ZIS7

Additional RARRES1 Products

Product Documents for Mouse RARRES1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse RARRES1 Antibody

For research use only

Customer Reviews for Mouse RARRES1 Antibody (1)

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  • Mouse RARRES1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Liver tissue, Kidney tissue, Lung tissue and Spleen tissue
    Species: Mouse
    Verified Customer | Posted 11/01/2016
    Mouse RARRES1 Antibody AF4657

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Protocols

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