Mouse RARRES1 Antibody

Catalog # Availability Size / Price Qty
AF4657
AF4657-SP
Detection of Mouse RARRES1 by Western Blot.
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Mouse RARRES1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse RARRES1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human RARRES1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse RARRES1
Pro42-Phe283
Accession # F6ZIS7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
Perfusion fixed frozen sections of mouse liver

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse RARRES1 antibody by Western Blot. View Larger

Detection of Mouse RARRES1 by Western Blot. Western blot shows lysates of mouse spleen tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse RARRES1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4657) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for RARRES1 at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RARRES1

RARRES1 (retinoic acid receptor responder 1; also Tig1) is a member of the protease inhibitor I47 family of proteins. It is expressed in epithelium, including keratinocytes, plus prostate and breast epithelium. It is considered to be a tumor-suppressor molecule, presumably acting as an inhibitor of carboxypeptidase A. Mouse RARRES1 is 283 amino acids (aa) in length. Based on human, it is a type II transmembrane (TM) protein with a TM segment at, or near, aa 75‑95. Over
aa 42-283, mouse RARRES1 shares 85% and 74% aa identity with rat and human RARRES1, respectively.

Long Name
Retinoic Acid Receptor Responder [Tazarotene-induced] 1
Entrez Gene IDs
5918 (Human); 109222 (Mouse); 310486 (Rat)
Alternate Names
RARRES1; RAR-responsive protein TIG1; retinoic acid receptor responder (tazarotene induced) 1; retinoic acid receptor responder protein 1; TIG1; TIG1Tazarotene-induced gene 1 protein

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Mouse RARRES1 Antibody
By Anonymous on 11/01/2016
Application: WB Sample Tested: Kidney tissue,Liver tissue,Lung tissue,Spleen tissue Species: Mouse