Mouse/Rat Carboxylesterase 3/CES3 Antibody

Catalog # Availability Size / Price Qty
AF5985
AF5985-SP

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Detection of Mouse and Rat Carboxylesterase 3/CES3 by Western Blot.
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Mouse/Rat Carboxylesterase 3/CES3 Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects recombinant mouse Carboxylesterase 3/CES3 in direct ELISAs and Western blots. Detects intrinsic CES3 in mouse and rat tissues in Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) CES1, rhCES2, recombinant mouse (rm) CES2, and rmCES5 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Carboxylesterase 3/CES3
Tyr19-Glu561
Accession # Q8VCT4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Mouse Carboxylesterase 3/CES3 (Catalog # 5428-CE), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse and Rat Carboxylesterase 3/CES3 antibody by Western Blot. View Larger

Detection of Mouse and Rat Carboxylesterase 3/CES3 by Western Blot. Western blot shows lysates of mouse lung tissue and rat lung tissue. PVDF Membrane was probed with 1 µg/mL of Mouse Carboxylesterase 3/CES3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5985) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Carboxylesterase 3/CES3 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carboxylesterase 3/CES3

Carboxylesterase 3 is a member of a large family of carboxylesterases that are responsible for the hydrolysis of ester and amide bonds (1, 2). These carboxylesterases are widely distributed in mammalian tissues and have broad substrate specificity ranging from small molecule esters such as phenylester to long-chain fatty acid esters and thioesters. Because many ester-containing drugs require the ester linkage to improve the bioavailability of therapeutic agents, CESs play a major role in drug metabolism and activation. CES3 is also known as triacylglycerol hydrolase for its lipolytic activity. It has been reported to be the major lipase in white adipose tissue (3). Mouse CES3 shares 93% homology with rat CES3.

References
  1. Redinbo, M. R. and Potter, P.M. (2005) Drug Discovery Today. 10:313.
  2. Satoh, T. and Hosokawa, M. (2006) Chem.-Biol. Interactions. 162:195.
  3. Soni, K. G. et al. (2004) J. Biol. Chem. 279:40683.
 
Entrez Gene IDs
23491 (Human); 104158 (Mouse); 113902 (Rat)
Alternate Names
carboxylesterase 3 (brain); Carboxylesterase 3; CES3; EC 3.1.1; EC 3.1.1.1; ES31FLJ21736; esterase 31; Liver carboxylesterase 31 homolog

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody

WB,ELISA

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