Mouse/Rat Jak2 Antibody

Name: Mouse/Rat Jak2 Antibody
Brand: R&D Systems
SKU: AF2988
Price: 159 USD
Availability: InStock

R&D Systems | Catalog # AF2988

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Cited:

Human, Rat

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

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Product Specifications

Immunogen

E. coli-derived recombinant mouse Jak2
Gly2-Val63
Accession # Q62120

Specificity

Detects endogenous mouse and rat Jak2 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse/Rat Jak2 Antibody

Detection of Mouse/Rat Jak2 by Western Blot.

Western blot shows lysates of DA3 mouse myeloma cell line, PT18 mouse mast/basophil cell line, and L6 rat myoblast cell lines. PVDF membrane was probed with 1 µg/mL of Mouse/Rat Jak2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2988) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Jak2 at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Mouse/Rat Jak2 Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: DA3 mouse myeloma cell line, PT18 mouse mast/basophil cell line, and L6 rat myoblast cell lines

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Jak2

Janus Kinase 2 (Jak2) belongs to a family of protein tyrosine kinases that couple to cytokine receptors and are activated by ligand binding to these receptors. Activation of Jak2 and other family members Jak1, Jak3, and Tyk2 occurs via phosphorylation at two adjacent tyrosine residues as well as potentially one or more serine residues. Jaks activate members of the STAT family of transcription factors by phosphorylating critical tyrosine regulatory sites. Jak2 plays an essential role in interferon gamma signaling.

Long Name

Janus Kinase 2

Alternate Names

EC 2.7.10, EC 2.7.10.2, JAK-2, Janus kinase 2Janus kinase 2 (a protein tyrosine kinase), JTK10, tyrosine-protein kinase JAK2

Entrez Gene IDs

3717 (Human)

Gene Symbol

JAK2

UniProt

Q62120

Additional Jak2 Products

Product Documents for Mouse/Rat Jak2 Antibody

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Certificate of Analysis

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Product Specific Notices for Mouse/Rat Jak2 Antibody

This product is sold under license from Millipore Corporation under the following US or foreign patents: 5,821,069; 5,658,791; EP0560890. This product shall not be used to commercially screen drug molecules being developed as JAK1 or JAK2 inhibitors. Any such activity will be outside the scope of the research use only label license.

For research use only