Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Cited:

Mouse, Rat, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 619
View all Tenascin R Primary Antibodies »

Product Specifications

Immunogen

“rest L2” fraction from bovine brain

Specificity

Detects mouse and rat Tenascin R in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Mouse/Rat Tenascin R Antibody

Tenascin R antibody in Rat Brain by Immunohistochemistry (IHC-Fr).

Tenascin R in Rat Brain.

Tenascin R was detected in perfusion fixed frozen sections of rat brain using Mouse Anti-Mouse/Rat Tenascin R Monoclonal Antibody (Catalog # MAB1624) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Detection of Mouse/Rat Tenascin R by Western Blot.

Western blot shows lysates of Mouse Brain and Rat Brain. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Mouse/Rat Tenascin R Monoclonal Antibody (Catalog # MAB1624) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Tenascin R at approximately 185-250 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Applications for Mouse/Rat Tenascin R Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Perfusion fixed frozen sections of rat brain (hippocampus)

Western Blot

2 µg/mL
Sample: Mouse Brain and Rat Brain

Reviewed Applications

Read 1 review rated 3 using MAB1624 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Tenascin R

Tenascin R is an extracellular matrix glycoprotein belonging to the Tenascin family of multidomain adhesion molecules. It is expressed exclusively in the central nervous system and affects neuronal cell migration and neurite extension. Mouse and rat Tenascin R share 97% amino acid sequence homology.

Alternate Names

Janusin, Restrictin, TNR

Entrez Gene IDs

7143 (Human); 21960 (Mouse); 25567 (Rat)

Gene Symbol

TNR

Additional Tenascin R Products

Product Documents for Mouse/Rat Tenascin R Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse/Rat Tenascin R Antibody

For research use only

Related Research Areas

Extracellular Matrix Molecules

Citations for Mouse/Rat Tenascin R Antibody

Customer Reviews for Mouse/Rat Tenascin R Antibody (1)

3 out of 5
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  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: See PMID 23069754
    Species: Mouse
    Verified Customer | Posted 02/10/2015

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Protocols

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