Mouse/Rat Tenascin R Antibody

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Tenascin R in Rat Brain.
2 Images
Product Details
Citations (5)
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Mouse/Rat Tenascin R Antibody Summary

Species Reactivity
Mouse, Rat
Detects mouse and rat Tenascin R in Western blots.
Monoclonal Mouse IgG1 Clone # 619
Protein A or G purified from hybridoma culture supernatant
“rest L2” fraction from bovine brain
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
Recombinant Mouse and Rat Tenascin R
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Tenascin R antibody in Rat Brain  by Immunohistochemistry (IHC-Fr). View Larger

Tenascin R in Rat Brain. Tenascin R was detected in perfusion fixed frozen sections of rat brain using Mouse Anti-Mouse/Rat Tenascin R Monoclonal Antibody (Catalog # MAB1624) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Western Blot View Larger

Detection of Mouse/Rat Tenascin R by Western Blot. Western blot shows lysates of Mouse Brain and Rat Brain. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Mouse/Rat Tenascin R Monoclonal Antibody (Catalog # MAB1624) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Tenascin R at approximately 185-250 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tenascin R

Tenascin R is an extracellular matrix glycoprotein belonging to the Tenascin family of multidomain adhesion molecules. It is expressed exclusively in the central nervous system and affects neuronal cell migration and neurite extension. Mouse and rat Tenascin R share 97% amino acid sequence homology.

Entrez Gene IDs
7143 (Human); 21960 (Mouse); 25567 (Rat)
Alternate Names
Janusin; MGC149328; Restrictin; tenascin R (restrictin, janusin); Tenascin R; tenascin-R; TNR; TN-R

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Citations for Mouse/Rat Tenascin R Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Protein-protein interactions between tenascin-R and RPTP?/phosphacan are critical to maintain the architecture of perineuronal nets
    Authors: Sinha, A;Kawakami, J;Cole, KS;Ladutska, A;Nguyen, MY;Zalmai, MS;Holder, BL;Broerman, VM;Matthews, RT;Bouyain, S;
    The Journal of biological chemistry
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  2. Antibody-directed extracellular proximity biotinylation reveals Contactin-1 regulates axo-axonic innervation of axon initial segments
    Authors: Y Ogawa, BC Lim, S George, JA Oses-Priet, JM Rasband, Y Eshed-Eise, S Nair, F Boato, E Peles, AL Burlingame, LV Aelst, MN Rasband
    bioRxiv : the preprint server for biology, 2023-03-06;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  3. Synapse type-specific proteomic dissection identifies IgSF8 as a hippocampal CA3 microcircuit organizer
    Authors: N Apóstolo, SN Smukowski, J Vanderlind, G Condomitti, V Rybakin, J Ten Bos, L Trobiani, S Portegies, KM Vennekens, NV Gounko, D Comoletti, KD Wierda, JN Savas, J de Wit
    Nat Commun, 2020-10-14;11(1):5171.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. The altered expression of perineuronal net elements during neural differentiation
    Authors: NF Eskici, S Erdem-Ozda, D Dayangac-E
    Cell. Mol. Biol. Lett., 2018-02-13;23(0):5.
    Species: Mouse, Rat
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  5. Quantitative Visualization of Dynamic Tracer Transportation in the Extracellular Space of Deep Brain Regions Using Tracer-Based Magnetic Resonance Imaging
    Authors: J Hou, W Wang, X Quan, W Liang, Z Li, D Chen, H Han
    Med. Sci. Monit., 2017-09-03;23(0):4260-4268.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot


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