Mouse/Rat Tenascin R Antibody
Mouse/Rat Tenascin R Antibody Summary
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Tenascin R in Rat Brain. Tenascin R was detected in perfusion fixed frozen sections of rat brain using Mouse Anti-Mouse/Rat Tenascin R Monoclonal Antibody (Catalog # MAB1624) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Detection of Mouse/Rat Tenascin R by Western Blot. Western blot shows lysates of Mouse Brain and Rat Brain. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Mouse/Rat Tenascin R Monoclonal Antibody (Catalog # MAB1624) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Tenascin R at approximately 185-250 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Tenascin R
Tenascin R is an extracellular matrix glycoprotein belonging to the Tenascin family of multidomain adhesion molecules. It is expressed exclusively in the central nervous system and affects neuronal cell migration and neurite extension. Mouse and rat Tenascin R share 97% amino acid sequence homology.
Citations for Mouse/Rat Tenascin R Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 5
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Protein-protein interactions between tenascin-R and RPTP?/phosphacan are critical to maintain the architecture of perineuronal nets
Authors: Sinha, A;Kawakami, J;Cole, KS;Ladutska, A;Nguyen, MY;Zalmai, MS;Holder, BL;Broerman, VM;Matthews, RT;Bouyain, S;
The Journal of biological chemistry
Sample Types: Whole Cells
Antibody-directed extracellular proximity biotinylation reveals Contactin-1 regulates axo-axonic innervation of axon initial segments
Authors: Y Ogawa, BC Lim, S George, JA Oses-Priet, JM Rasband, Y Eshed-Eise, S Nair, F Boato, E Peles, AL Burlingame, LV Aelst, MN Rasband
bioRxiv : the preprint server for biology, 2023-03-06;0(0):.
Sample Types: Whole Cells
Synapse type-specific proteomic dissection identifies IgSF8 as a hippocampal CA3 microcircuit organizer
Authors: N Apóstolo, SN Smukowski, J Vanderlind, G Condomitti, V Rybakin, J Ten Bos, L Trobiani, S Portegies, KM Vennekens, NV Gounko, D Comoletti, KD Wierda, JN Savas, J de Wit
Nat Commun, 2020-10-14;11(1):5171.
Sample Types: Whole Tissue
The altered expression of perineuronal net elements during neural differentiation
Authors: NF Eskici, S Erdem-Ozda, D Dayangac-E
Cell. Mol. Biol. Lett., 2018-02-13;23(0):5.
Species: Mouse, Rat
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot
Quantitative Visualization of Dynamic Tracer Transportation in the Extracellular Space of Deep Brain Regions Using Tracer-Based Magnetic Resonance Imaging
Authors: J Hou, W Wang, X Quan, W Liang, Z Li, D Chen, H Han
Med. Sci. Monit., 2017-09-03;23(0):4260-4268.
Sample Types: Tissue Homogenates
Applications: Western Blot
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