Mouse/Rat Tenascin R Antibody

Catalog #: MAB1624
5 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1624
MAB1624-SP

SAVE +FREE SHIPPING with ACADEMIA23 (See Promo Details)

2 Images

View All Products for Tenascin R

Product Details

Citations (5)

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse/Rat Tenascin R Antibody Summary

Species Reactivity

Mouse, Rat

Specificity

Detects mouse and rat Tenascin R in Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 619

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

“rest L2” fraction from bovine brain

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse and Rat Tenascin R

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

View Larger

Tenascin R in Rat Brain. Tenascin R was detected in perfusion fixed frozen sections of rat brain using Mouse Anti-Mouse/Rat Tenascin R Monoclonal Antibody (Catalog # MAB1624) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Western Blot

View Larger

Detection of Mouse/Rat Tenascin R by Western Blot. Western blot shows lysates of Mouse Brain and Rat Brain. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Mouse/Rat Tenascin R Monoclonal Antibody (Catalog # MAB1624) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Tenascin R at approximately 185-250 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tenascin R

Tenascin R is an extracellular matrix glycoprotein belonging to the Tenascin family of multidomain adhesion molecules. It is expressed exclusively in the central nervous system and affects neuronal cell migration and neurite extension. Mouse and rat Tenascin R share 97% amino acid sequence homology.

Entrez Gene IDs

7143 (Human); 21960 (Mouse); 25567 (Rat)

Alternate Names

Janusin; MGC149328; Restrictin; tenascin R (restrictin, janusin); Tenascin R; tenascin-R; TNR; TN-R

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Extracellular Matrix Molecules

Citations for Mouse/Rat Tenascin R Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
Filter your results:

Filter by:

Protein-protein interactions between tenascin-R and RPTP?/phosphacan are critical to maintain the architecture of perineuronal nets
Authors: Sinha, A;Kawakami, J;Cole, KS;Ladutska, A;Nguyen, MY;Zalmai, MS;Holder, BL;Broerman, VM;Matthews, RT;Bouyain, S;
The Journal of biological chemistry
Species: Mouse
Sample Types: Whole Cells
Applications: ICC
Antibody-directed extracellular proximity biotinylation reveals Contactin-1 regulates axo-axonic innervation of axon initial segments
Authors: Y Ogawa, BC Lim, S George, JA Oses-Priet, JM Rasband, Y Eshed-Eise, S Nair, F Boato, E Peles, AL Burlingame, LV Aelst, MN Rasband
bioRxiv : the preprint server for biology, 2023-03-06;0(0):.
Species: Rat
Sample Types: Whole Cells
Applications: ICC
Synapse type-specific proteomic dissection identifies IgSF8 as a hippocampal CA3 microcircuit organizer
Authors: N Apóstolo, SN Smukowski, J Vanderlind, G Condomitti, V Rybakin, J Ten Bos, L Trobiani, S Portegies, KM Vennekens, NV Gounko, D Comoletti, KD Wierda, JN Savas, J de Wit
Nat Commun, 2020-10-14;11(1):5171.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
The altered expression of perineuronal net elements during neural differentiation
Authors: NF Eskici, S Erdem-Ozda, D Dayangac-E
Cell. Mol. Biol. Lett., 2018-02-13;23(0):5.
Species: Mouse, Rat
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot
Quantitative Visualization of Dynamic Tracer Transportation in the Extracellular Space of Deep Brain Regions Using Tracer-Based Magnetic Resonance Imaging
Authors: J Hou, W Wang, X Quan, W Liang, Z Li, D Chen, H Han
Med. Sci. Monit., 2017-09-03;23(0):4260-4268.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot