Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all RELM beta Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse RELM beta
Gly21-Ala105
Accession # Q99P86

Specificity

Detects mouse RELM beta in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant rat RELM gamma is observed and 10% cross-reactivity with recombinant mouse (rm) Resistin and rmRELM alpha is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse RELM beta Antibody

Detection of Mouse RELM beta antibody by Western Blot.

Detection of Mouse RELM beta by Western Blot.

Western blot shows lysates of mouse small intestine tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse RELM beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2730) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for RELM beta at approximately 19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Mouse RELM beta Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Mouse small intestine tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: RELM beta

RELM beta, also known as FIZZ2 (found in inflammatory zone 2) is a 9 kDa secreted, cysteine-rich protein belonging to the RELM family. By analogy to Resistin, RELM beta is expected to exist in two distinct multimeric structures corresponding to hexamers and trimers. RELM beta is synthesized by intestinal goblet cells and is secreted into the intestinal lumen. Mouse RELM beta shares 61% and 77% amino acid (aa) sequence identity with human and rat RELM beta, respectively. It also shares 52%, 71% and 46% aa sequence identity with mouse RELM alpha, RELM gamma and Resistin, respectively.

Long Name

Resistin-like Molecule beta

Alternate Names

C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich proteinprecursor 2, CCRG, Colon and small intestine-specific cysteine-rich protein, Colon carcinoma-related gene protein, cysteine-rich secreted A12-alpha-like protein 1, Cysteine-rich secreted protein A12-alpha-like 1, Cysteine-rich secreted protein FIZZ2, FIZZ1, FIZZ2RELM-beta, found in inflammatory zone 1, HXCP2XCP2, RELMb, RELMbeta, resistin like beta, resistin-like beta, RETNL2

Entrez Gene IDs

84666 (Human); 57263 (Mouse)

Gene Symbol

RETNLB

UniProt

Q99P86

Additional RELM beta Products

Product Documents for Mouse RELM beta Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse RELM beta Antibody

For research use only

Related Research Areas

Obesity

Citations for Mouse RELM beta Antibody

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