Mouse RELM beta Antibody Summary
Accession # Q99P86
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Mouse RELM beta by Western Blot. Western blot shows lysates of mouse small intestine tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse RELM beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2730) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for RELM beta at approximately 19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: RELM beta
RELM beta, also known as FIZZ2 (found in inflammatory zone 2) is a 9 kDa secreted, cysteine-rich protein belonging to the RELM family. By analogy to Resistin, RELM beta is expected to exist in two distinct multimeric structures corresponding to hexamers and trimers. RELM beta is synthesized by intestinal goblet cells and is secreted into the intestinal lumen. Mouse RELM beta shares 61% and 77% amino acid (aa) sequence identity with human and rat RELM beta, respectively. It also shares 52%, 71% and 46% aa sequence identity with mouse RELM alpha, RELM gamma and Resistin, respectively.
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