Detects mouse S100A13 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 40% cross-reactivity with recombinant human (rh) S100A13 is observed, and less than 1% cross-reactivity with rhS100A1 and rhS100A11 is observed.
Polyclonal Goat IgG
E. coli-derived recombinant mouse S100A13 Ala2-Lys98 Accession # P97352
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
S100A13 in Mouse Embryo. S100A13 was detected in immersion fixed frozen sections of mouse embryo using 15 µg/mL Goat Anti-Mouse S100A13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4328) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
S100A13 is an 11 kDa member of the S100 (soluble in 100% saturated ammonium sulfate) family of vertebrate EF-hand Ca++-binding proteins (1-3). It is widely expressed as a homodimer with 98 amino acid (aa) long subunits (2, 3). Mouse S100A13 shares 87%, 83%, 91%, 86%, 81%, and 53% aa identity with rat, human, bovine, canine, opossum, and chicken S100A13, respectively. Like other S100 proteins, S100A13 is small and generally acidic, but it contains a basic residue-rich sequence at the C-terminus, and two EF hand motifs that bind Ca++ with differing affinities (2-4). Some S100 proteins, including S100A13, are able to bind the cell surface receptor for advanced glycation end-products (RAGE) (5). Despite lacking a signal sequence, S100A13 plays an important role in Cu++-dependent export of FGF-1 (FGF acidic) and IL-1 alpha from the cell in response to stresses such as heat shock, anoxia, and starvation (6-8). Binding of copper is necessary for formation of a multi-protein complex between S100A13, FGF-1 and p40 synaptotagmin-1 (syt-1) (9, 10). Cu++ ions supplied by S100A13 are thought to oxidize and downregulate the activity of FGF-1 prior to export (10). Calcium influx may also play a similar role in FGF-1 release from neuronal cells (11). S100A13 is composed of four amphiphilic helices that may interact with acidic phospholipid headgroups. With FGF-1 and syt-1, S100A13 likely perturbs the membrane, which allows the S100A13 protein complex to exit the cell (4, 12). S100A13 has been proposed as a marker for angiogenesis in tumors and endometrium, due to its role in stress-induced export of FGF‑1 (13, 14). Based on in house studies, S100A13 has also been found to promote neurite outgrowth from rat cortical embryonic neurons (15).
Santamaria-Kisiel, L. et al. (2006) Biochem. J. 396:201.
Wicki, R. et al. (1996) Biochem. Biophys. Res. Commun. 227:594.
Ridinger, K. et al. (2000) J. Biol. Chem. 275:8686.
Li, M. et al. (2007) Biochem. Biophys. Res. Commun. 356:616.
Hsieh, H-L. et al. (2004) Biochem. Biophys. Res. Commun. 316:949.
Landriscina, M. et al. (2001) J. Biol. Chem. 276:22544.
Sivaraja, V. et al. (2006) Biophys. J. 91:1832.
Mandinova, A. et al. (2003) J. Cell Sci. 116:2687.
Prudovsky, I. et al. (2002) J. Cell Biol. 158:201.
Landriscina, M. et al. (2001) J. Biol. Chem. 276:25549.
Matsunaga, H. and H. Ueda (2006) Cell. Mol. Neurobiol. 26:237.
Graziani, I. et al. (2006) Biochem. Biophys. Res. Commun. 349:192.
Landriscina, M. et al. (2006) J. Neurooncol. 80:251.
Hayrabedyan, S. et al. (2005) Reprod. Biol. 5:51.
R&D Sytems (2007) In-house data.
S100 Calcium Binding Protein
Entrez Gene IDs:
6284 (Human); 20196 (Mouse); 295213 (Rat)
S100 calcium binding protein A13; S100 calcium-binding protein A13protein S100-A13; S100A13
Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.