Mouse S5a/Angiocidin Antibody

(1 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse S5a/Angiocidin in direct ELISAs and Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant mouse S5a/Angiocidin isoform A
    Val2-Glu254
    Accession # O35226
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse S5a/Angiocidin by Western Blot. Western blot shows lysates of 3T3‑L1 mouse embryonic fibroblast adipose‑like cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse S5a/Angiocidin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5540) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for S5a/Angiocidin at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: S5a/Angiocidin

Angiocidin (also S5a, 26S proteasome regulatory subunit S5A, Psmd4, RPN10a and Mcb1) is a 41 kDa (predicted) member of the proteosome subunit S5A family of proteins. It runs anomalously in SDS-PAGE at 55 kDa. Intracellularly, Angiocidin is a component of the 26S proteosome that degrades ubiquitinated proteins. Extracellularly, it inhibits angiogenesis by binding to collagen and beta 1 integrins. Mouse Angiocidin is 376 amino acids (aa) in length. It contains a vWF-A domain (aa 5‑188), a beta 1 interacting site (aa 86‑105) and two ubiquitin-interacting motifs (aa 211‑230 and 282‑301). There are four splice variants. Three show a GlyGluArg insertion after Glu254, with two of these showing an additional three aa substitution for aa 255‑376, and a 45 aa substitution for aa 321‑376, respectfully. A fourth shows a 51 aa substitution for aa 299‑376. Over aa 1‑254, mouse Angiocidin shares > 99% aa identity with rat and human Angiocidin.

  • Long Name:
    26S Proteasome Regulatory Subunit S5a
  • Entrez Gene IDs:
    5710 (Human); 19185 (Mouse); 83499 (Rat)
  • Alternate Names:
    Angiocidin; ASF; Macropain; Mcb1; PSMD4; pUB-R5; Rpn10 ; S5a
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. The autism-linked UBE3A T485A mutant E3 ubiquitin ligase activates the Wnt/?-catenin pathway by inhibiting the proteasome
    Authors: JJ Yi, SR Paranjape, MP Walker, R Choudhury, JM Wolter, G Fragola, MJ Emanuele, MB Major, MJ Zylka
    J. Biol. Chem., 2017;0(0):.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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