|Detection of Mouse S5a/Angiocidin by Western Blot. Western blot shows lysates of 3T3‑L1 mouse embryonic fibroblast adipose‑like cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse S5a/Angiocidin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5540) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for S5a/Angiocidin at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.|
Angiocidin (also S5a, 26S proteasome regulatory subunit S5A, Psmd4, RPN10a and Mcb1) is a 41 kDa (predicted) member of the proteosome subunit S5A family of proteins. It runs anomalously in SDS-PAGE at 55 kDa. Intracellularly, Angiocidin is a component of the 26S proteosome that degrades ubiquitinated proteins. Extracellularly, it inhibits angiogenesis by binding to collagen and beta 1 integrins. Mouse Angiocidin is 376 amino acids (aa) in length. It contains a vWF-A domain (aa 5‑188), a beta 1 interacting site (aa 86‑105) and two ubiquitin-interacting motifs (aa 211‑230 and 282‑301). There are four splice variants. Three show a GlyGluArg insertion after Glu254, with two of these showing an additional three aa substitution for aa 255‑376, and a 45 aa substitution for aa 321‑376, respectfully. A fourth shows a 51 aa substitution for aa 299‑376. Over aa 1‑254, mouse Angiocidin shares > 99% aa identity with rat and human Angiocidin.
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