Mouse S5a/Angiocidin Antibody Summary
Accession # O35226
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Mouse S5a/Angiocidin by Western Blot. Western blot shows lysates of 3T3‑L1 mouse embryonic fibroblast adipose‑like cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse S5a/Angiocidin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5540) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for S5a/Angiocidin at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Angiocidin (also S5a, 26S proteasome regulatory subunit S5A, Psmd4, RPN10a and Mcb1) is a 41 kDa (predicted) member of the proteosome subunit S5A family of proteins. It runs anomalously in SDS-PAGE at 55 kDa. Intracellularly, Angiocidin is a component of the 26S proteosome that degrades ubiquitinated proteins. Extracellularly, it inhibits angiogenesis by binding to collagen and beta 1 integrins. Mouse Angiocidin is 376 amino acids (aa) in length. It contains a vWF-A domain (aa 5‑188), a beta 1 interacting site (aa 86‑105) and two ubiquitin-interacting motifs (aa 211‑230 and 282‑301). There are four splice variants. Three show a GlyGluArg insertion after Glu254, with two of these showing an additional three aa substitution for aa 255‑376, and a 45 aa substitution for aa 321‑376, respectfully. A fourth shows a 51 aa substitution for aa 299‑376. Over aa 1‑254, mouse Angiocidin shares > 99% aa identity with rat and human Angiocidin.
Citation for Mouse S5a/Angiocidin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
The autism-linked UBE3A T485A mutant E3 ubiquitin ligase activates the Wnt/?-catenin pathway by inhibiting the proteasome
Authors: JJ Yi, SR Paranjape, MP Walker, R Choudhury, JM Wolter, G Fragola, MJ Emanuele, MB Major, MJ Zylka
J. Biol. Chem., 2017;0(0):.
Sample Types: Cell Lysates
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