Mouse SPARC Antibody

(11 citations)
(1 Review)
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse SPARC in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human SPARC is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse SPARC/Osteonectin
    Ala18-Ile302
    Accession # P07214
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.2 µg/mL
    See below
  • Immunohistochemistry
    1-15 µg/mL
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Intracellular Staining by Flow Cytometry
    0.25 µg/106 cells
    Balb/3T3 mouse embryonic fibroblast cell line fixed with paraformaldehyde and permeabilized with saponin
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse SPARC by Western Blot. Western blot shows lysates of C2C12 mouse myoblast cell line and mouse placenta tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse SPARC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF942) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for SPARC at approximately 35-37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
SPARC/Osteonectin in Mouse Embryo. SPARC/Osteonectin was detected in immersion fixed frozen sections of mouse embryo (E15) using Mouse SPARC/
Osteonectin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF942) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to developing cartilage. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SPARC

SPARC, an acronym for “secreted protein, acidic and rich in cysteine”, is also known as osteonectin or BM-40 (1-5). It is the founding member of a family of secreted matricellular proteins with similar domain structure. The 302 amino acid (aa), 43 kDa protein contains a 17 aa signal sequence, an N-terminal acidic region that binds calcium, a follistatin domain containing Kazal-like sequences, and a C-terminal extracellular calcium (EC) binding domain with two EF-hand motifs (1-5). Crystal structure shows that residues implicated in cell binding, inhibition of cell spreading and disassembly of focal adhesions cluster on one face of SPARC, while a collagen binding epitope and an N-glycosylation site are opposite this face (6). SPARC is produced by fibroblasts, capillary endothelial cells, platelets, and macrophages, especially in areas of tissue morphogenesis and remodeling (3, 7). SPARC shows context-specific effects, but generally inhibits adhesion, spreading and proliferation, and promotes collagen matrix formation (3-5). For endothelial cells, SPARC disrupts focal adhesions and binds and sequesters PDGF and VEGF (3-5). SPARC is abundantly expressed in bone, where it promotes osteoblast differentiation and inhibits adipogenesis (5, 8). SPARC is potentially cleaved by metalloproteinases, producing an angiogenic peptide that includes the copper-binding sequence KGHK (7). Paradoxically, SPARC is highly expressed in many tumor types, yet expression mainly decreases the likelihood of metastasis and confers sensitivity to chemotherapy and radiation (4, 9, 10). Stabilin-1, which is expressed on alternately activated macrophages, is the first SPARC receptor to be identified. It binds the SPARC EC domain and mediates endocytosis for degradation (11). Mature mouse SPARC shows 97%, 92%, 92%, 92%, and 83% aa identity with rat, human, dog, cow, and chick SPARC, respectively.

  • References:
    1. Lankat-Buttgereit, B. et al. (1988) FEBS Lett. 236:352.
    2. McVey, J.H. et al. (1988) J. Biol. Chem. 263:11111.
    3. Sage, H. et al. (1989) J. Cell Biol. 109:341.
    4. Framson, P.E. and E.H. Sage (2004) J. Cell. Biochem. 92:679.
    5. Alford, A.I. and K.D. Hankenson (2006) Bone 38:749.
    6. Hohenester, E. et al. (1997) EMBO J. 16:3778.
    7. Sage, E.H. et al. (2003) J. Biol. Chem. 278:37849.
    8. Delany, A.M. et al. (2003) Endocrinology 144:2588.
    9. Koblinski, J.E. et al. (2005) Cancer Res. 65:7370.
    10. Tai, I.T. et al. (2005) J. Clin. Invest. 115:1492.
    11. Kzhyshkowska, J. et al. (2006) J. Immunol. 176:5825.
  • Long Name:
    Secreted Protein Acidic and Rich in Cysteine
  • Entrez Gene IDs:
    6678 (Human); 20692 (Mouse)
  • Alternate Names:
    Basement-membrane protein 40; BM-40; ONcysteine-rich protein; Osteonectin; Secreted protein acidic and rich in cysteine; secreted protein, acidic, cysteine-rich (osteonectin); SPARC
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Molecular and Functional Properties of Regional Astrocytes in the Adult Brain
    Authors: L Morel, MSR Chiang, H Higashimor, T Shoneye, LK Iyer, J Yelick, A Tai, Y Yang
    J. Neurosci., 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  2. Stabilin-1 is expressed in human breast cancer and supports tumor growth in mammary adenocarcinoma mouse model
    Oncotarget, 2016;7(21):31097-110.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  3. CSF1-ETS2-induced microRNA in myeloid cells promote metastatic tumor growth.
    Authors: Mathsyaraja H, Thies K, Taffany D, Deighan C, Liu T, Yu L, Fernandez S, Shapiro C, Otero J, Timmers C, Lustberg M, Chalmers J, Leone G, Ostrowski M
    Oncogene, 2015;34(28):3651-61.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  4. Proteolytic Isoforms of SPARC Induce Adipose Stromal Cell Mobilization in Obesity.
    Authors: Tseng C, Kolonin M
    Stem Cells, 2015;34(1):174-90.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  5. Stromal niche communalities underscore the contribution of the matricellular protein SPARC to B-cell development and lymphoid malignancies.
    Authors: Sangaletti, Sabina, Tripodo, Claudio, Portararo, Paola, Dugo, Matteo, Vitali, Caterina, Botti, Laura, Guarnotta, Carla, Cappetti, Barbara, Gulino, Alessand, Torselli, Ilaria, Casalini, Patrizia, Chiodoni, Claudia, Colombo, Mario P
    Oncoimmunology, 2014;3(0):e28989.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Not Specified
  6. Losartan slows pancreatic tumor progression and extends survival of SPARC-null mice by abrogating aberrant TGF beta activation.
    Authors: Arnold SA, Rivera LB, Carbon JG, Toombs JE, Chang CL, Bradshaw AD, Brekken RA
    PLoS ONE, 2012;7(2):e31384.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  7. Astrocytes control glutamate receptor levels at developing synapses through SPARC-beta-integrin interactions.
    Authors: Jones EV, Bernardinelli Y, Tse YC, Chierzi S, Wong TP, Murai KK
    J. Neurosci., 2011;31(11):4154-65.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  8. Absence of SPARC results in increased cardiac rupture and dysfunction after acute myocardial infarction.
    Authors: Schellings MW, Vanhoutte D, Swinnen M, Cleutjens JP, Debets J, van Leeuwen RE, d'Hooge J, Van de Werf F, Carmeliet P, Pinto YM, Sage EH, Heymans S
    J. Exp. Med., 2009;206(1):113-23.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  9. A conserved organization of transcription during embryonic stem cell differentiation and in cells with high C value.
    Authors: Faro-Trindade I, Cook PR
    Mol. Biol. Cell, 2006;17(7):2910-20.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  10. Loss of SPARC-mediated VEGFR-1 suppression after injury reveals a novel antiangiogenic activity of VEGF-A.
    Authors: Nozaki M, Sakurai E, Raisler BJ, Baffi JZ, Witta J, Ogura Y, Brekken RA, Sage EH, Ambati BK, Ambati J
    J. Clin. Invest., 2006;116(2):422-9.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  11. Functional analysis of the matricellular protein SPARC with novel monoclonal antibodies.
    Authors: Sweetwyne MT, Brekken RA, Workman G, Bradshaw AD, Carbon J, Siadak AW, Murri C, Sage EH
    J. Histochem. Cytochem., 2004;52(6):723-33.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: WB
Expand to show all 11 Citations
Cell and Tissue Staining Kits
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Immunohistochemistry Reagents
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VisUCyte Antigen Retrieval Reagent-Basic

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Isotype Controls
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Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
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Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

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Goat IgG (H+L) PE-conjugated Antibody

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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

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Goat IgG (H+L) APC-conjugated Antibody

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Goat IgG Horseradish Peroxidase-conjugated Antibody

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Donkey Anti-Goat IgG Antibody

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Donkey Anti-Goat IgG Biotinylated Antibody

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Goat IgG VisUCyte HRP Polymer

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Goat IgG (H+L) Fluorescein-conjugated Antibody

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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

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Chicken Anti-Goat IgG Biotinylated Antibody

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Rabbit Anti-Goat IgG Biotinylated Antibody

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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

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Average Rating: 4 (Based on 1 review)

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We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: Immunohistochemistry.

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Images Ratings Applications Species Reviewed By Date Details
Immunohistochemistry Mouse SPARC Antibody AF942
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Very Good
 IHC Mouse Anonymous 05/18/2017
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Immunohistochemistry Mouse SPARC Antibody AF942
Immunohistochemistry: Mouse SPARC Antibody [AF942]

Summary

ApplicationImmunohistochemistry
Sample TestedColon cancer tissue,Colon tissue
SpeciesMouse

Other Experimental Details

Other Experimental DetailsWorked well in methacarn fixed tissues

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