Mouse SPARC Antibody

Catalog # Availability Size / Price Qty
AF942
AF942-SP
Mouse SPARC Antibody in Western Blot
3 Images
Product Details
Citations (17)
FAQs
Supplemental Products
Reviews (3)

Mouse SPARC Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse SPARC in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human SPARC is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse SPARC/Osteonectin
Ala18-Ile302
Accession # P07214
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below
Immunohistochemistry
1-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
See below
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
Balb/3T3 mouse embryonic fibroblast cell line fixed with paraformaldehyde and permeabilized with saponin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Mouse SPARC by Western Blot. View Larger

Detection of Mouse SPARC by Western Blot. Western blot shows lysates of C2C12 mouse myoblast cell line and mouse placenta tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse SPARC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF942) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for SPARC at approximately 35-37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry SPARC/Osteonectin in Mouse Embryo. View Larger

SPARC/Osteonectin in Mouse Embryo. SPARC/Osteonectin was detected in immersion fixed frozen sections of mouse embryo (E15) using Mouse SPARC/ Osteonectin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF942) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to developing cartilage. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Immunocytochemistry SPARC in C2C12 Mouse Cell Line. View Larger

SPARC in C2C12 Mouse Cell Line. SPARC was detected in immersion fixed C2C12 mouse myoblast cell line using Goat Anti-Mouse SPARC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF942) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to endoplasmic reticuli. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SPARC

SPARC, an acronym for “secreted protein, acidic and rich in cysteine”, is also known as osteonectin or BM-40 (1-5). It is the founding member of a family of secreted matricellular proteins with similar domain structure. The 302 amino acid (aa), 43 kDa protein contains a 17 aa signal sequence, an N-terminal acidic region that binds calcium, a follistatin domain containing Kazal-like sequences, and a C-terminal extracellular calcium (EC) binding domain with two EF-hand motifs (1-5). Crystal structure shows that residues implicated in cell binding, inhibition of cell spreading and disassembly of focal adhesions cluster on one face of SPARC, while a collagen binding epitope and an N-glycosylation site are opposite this face (6). SPARC is produced by fibroblasts, capillary endothelial cells, platelets, and macrophages, especially in areas of tissue morphogenesis and remodeling (3, 7). SPARC shows context-specific effects, but generally inhibits adhesion, spreading and proliferation, and promotes collagen matrix formation (3-5). For endothelial cells, SPARC disrupts focal adhesions and binds and sequesters PDGF and VEGF (3-5). SPARC is abundantly expressed in bone, where it promotes osteoblast differentiation and inhibits adipogenesis (5, 8). SPARC is potentially cleaved by metalloproteinases, producing an angiogenic peptide that includes the copper-binding sequence KGHK (7). Paradoxically, SPARC is highly expressed in many tumor types, yet expression mainly decreases the likelihood of metastasis and confers sensitivity to chemotherapy and radiation (4, 9, 10). Stabilin-1, which is expressed on alternately activated macrophages, is the first SPARC receptor to be identified. It binds the SPARC EC domain and mediates endocytosis for degradation (11). Mature mouse SPARC shows 97%, 92%, 92%, 92%, and 83% aa identity with rat, human, dog, cow, and chick SPARC, respectively.

References
  1. Lankat-Buttgereit, B. et al. (1988) FEBS Lett. 236:352.
  2. McVey, J.H. et al. (1988) J. Biol. Chem. 263:11111.
  3. Sage, H. et al. (1989) J. Cell Biol. 109:341.
  4. Framson, P.E. and E.H. Sage (2004) J. Cell. Biochem. 92:679.
  5. Alford, A.I. and K.D. Hankenson (2006) Bone 38:749.
  6. Hohenester, E. et al. (1997) EMBO J. 16:3778.
  7. Sage, E.H. et al. (2003) J. Biol. Chem. 278:37849.
  8. Delany, A.M. et al. (2003) Endocrinology 144:2588.
  9. Koblinski, J.E. et al. (2005) Cancer Res. 65:7370.
  10. Tai, I.T. et al. (2005) J. Clin. Invest. 115:1492.
  11. Kzhyshkowska, J. et al. (2006) J. Immunol. 176:5825.
Long Name
Secreted Protein Acidic and Rich in Cysteine
Entrez Gene IDs
6678 (Human); 20692 (Mouse)
Alternate Names
Basement-membrane protein 40; BM-40; ONcysteine-rich protein; Osteonectin; Secreted protein acidic and rich in cysteine; secreted protein, acidic, cysteine-rich (osteonectin); SPARC

Product Datasheets

Citations for Mouse SPARC Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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  1. Differential Proliferation and Maturation of Subcortical Astrocytes During Postnatal Development
    Authors: T Shoneye, AT Orrego, R Jarvis, Y Men, MSR Chiang, Y Yang
    Front Neurosci, 2020;14(0):435.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Bim expression modulates the pro-inflammatory phenotype of retinal astroglial cells
    Authors: J Falero-Per, N Sheibani, CM Sorenson
    PLoS ONE, 2020;15(5):e0232779.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. SPARC dependent collagen deposition and gemcitabine delivery in a genetically engineered mouse model of pancreas cancer
    Authors: I Ramu, SM Buchholz, MS Patzak, RG Goetze, SK Singh, FM Richards, DI Jodrell, B Sipos, P Ströbel, V Ellenriede, E Hessmann, A Neesse
    EBioMedicine, 2019;0(0):.
  4. Extracellular SPARC increases cardiomyocyte contraction during health and disease
    Authors: S Deckx, DM Johnson, M Rienks, P Carai, E Van Deel, J Van der Ve, KR Sipido, S Heymans, AP Papageorgi
    PLoS ONE, 2019;14(4):e0209534.
    Species: Mouse
    Sample Types: Cell Fraction
    Applications: Western Blot
  5. Cyp1b1 expression impacts the angiogenic and inflammatory properties of liver sinusoidal endothelial cells
    Authors: J Falero-Per, YS Song, Y Zhao, L Teixeira, CM Sorenson, N Sheibani
    PLoS ONE, 2018;13(10):e0206756.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. SPARC and GluA1-Containing AMPA Receptors Promote Neuronal Health Following CNS Injury
    Authors: EV Jones, Y Bernardine, JG Zarruk, S Chierzi, KK Murai
    Front Cell Neurosci, 2018;12(0):22.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  7. Molecular and Functional Properties of Regional Astrocytes in the Adult Brain
    Authors: L Morel, MSR Chiang, H Higashimor, T Shoneye, LK Iyer, J Yelick, A Tai, Y Yang
    J. Neurosci., 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. Stabilin-1 is expressed in human breast cancer and supports tumor growth in mammary adenocarcinoma mouse model
    Oncotarget, 2016;7(21):31097-110.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Proteolytic Isoforms of SPARC Induce Adipose Stromal Cell Mobilization in Obesity.
    Authors: Tseng C, Kolonin M
    Stem Cells, 2015;34(1):174-90.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  10. CSF1-ETS2-induced microRNA in myeloid cells promote metastatic tumor growth.
    Authors: Mathsyaraja H, Thies K, Taffany D, Deighan C, Liu T, Yu L, Fernandez S, Shapiro C, Otero J, Timmers C, Lustberg M, Chalmers J, Leone G, Ostrowski M
    Oncogene, 2015;34(28):3651-61.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. Stromal niche communalities underscore the contribution of the matricellular protein SPARC to B-cell development and lymphoid malignancies.
    Authors: Sangaletti, Sabina, Tripodo, Claudio, Portararo, Paola, Dugo, Matteo, Vitali, Caterina, Botti, Laura, Guarnotta, Carla, Cappetti, Barbara, Gulino, Alessand, Torselli, Ilaria, Casalini, Patrizia, Chiodoni, Claudia, Colombo, Mario P
    Oncoimmunology, 2014;3(0):e28989.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  12. Losartan slows pancreatic tumor progression and extends survival of SPARC-null mice by abrogating aberrant TGF beta activation.
    Authors: Arnold SA, Rivera LB, Carbon JG, Toombs JE, Chang CL, Bradshaw AD, Brekken RA
    PLoS ONE, 2012;7(2):e31384.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  13. Astrocytes control glutamate receptor levels at developing synapses through SPARC-beta-integrin interactions.
    Authors: Jones EV, Bernardinelli Y, Tse YC, Chierzi S, Wong TP, Murai KK
    J. Neurosci., 2011;31(11):4154-65.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  14. Absence of SPARC results in increased cardiac rupture and dysfunction after acute myocardial infarction.
    Authors: Schellings MW, Vanhoutte D, Swinnen M, Cleutjens JP, Debets J, van Leeuwen RE, d'Hooge J, Van de Werf F, Carmeliet P, Pinto YM, Sage EH, Heymans S
    J. Exp. Med., 2009;206(1):113-23.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  15. A conserved organization of transcription during embryonic stem cell differentiation and in cells with high C value.
    Authors: Faro-Trindade I, Cook PR
    Mol. Biol. Cell, 2006;17(7):2910-20.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  16. Loss of SPARC-mediated VEGFR-1 suppression after injury reveals a novel antiangiogenic activity of VEGF-A.
    Authors: Nozaki M, Sakurai E, Raisler BJ, Baffi JZ, Witta J, Ogura Y, Brekken RA, Sage EH, Ambati BK, Ambati J
    J. Clin. Invest., 2006;116(2):422-9.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  17. Functional analysis of the matricellular protein SPARC with novel monoclonal antibodies.
    Authors: Sweetwyne MT, Brekken RA, Workman G, Bradshaw AD, Carbon J, Siadak AW, Murri C, Sage EH
    J. Histochem. Cytochem., 2004;52(6):723-33.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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Reviews for Mouse SPARC Antibody

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Mouse SPARC Antibody
By Anonymous on 02/02/2018
Application: WB Sample Tested: Mouse hippocampal tissue Species: Mouse

Used at 1:3000 in 5% BSA and secondary goat-HRP antibody (R&D) at 1:5000


Mouse SPARC Antibody
By Anonymous on 01/12/2018
Application: WB Sample Tested: trabecular meshwork Species: Mouse

mouse SPARC was overexpressed by adeno-SPARC in mouse trabecular meshork (TM) cells.


Mouse SPARC Antibody
By Anonymous on 05/18/2017
Application: IHC Sample Tested: Colon cancer tissue,Colon tissue Species: Mouse

Worked well in methacarn fixed tissues