Mouse TIGIT Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB72671-100
MAB72671-SP
Mouse TIGIT Antibody in Data
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Product Details
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Mouse TIGIT Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse TIGIT in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2190A
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse TIGIT
Met1-Thr143
Accession # P86176
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of TIGIT on CD4+ Mouse Splenocytes by Flow Cytometry. View Larger

Detection of TIGIT on CD4+ Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were surface stained with Rat anti-Mouse CD4 FITC-conjugated Monoclonal Antibody (Catalog # FAB554F) and either (A) Rabbit Anti-Mouse TIGIT Monoclonal Antibody (Catalog # MAB72671) or (B) Rabbit IgG control antibody (Catalog # MAB1050) followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). Then stained with Rabbit anti-Human/Mouse FoxP3 PE-conjugated Monoclonal Antibody (Catalog # IC8214P).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TIGIT

TIGIT (T cell Immunoreceptor with Ig and ITIM domains; also Vstm3 and Vsig9) is a 30-34 kDa (in human) member of the CD28 family, Ig superfamily of molecules. It is expressed by NK cells and multiple subsets of mature T cells, and binds to PVR/CD155 and PVR2/CD112 that appear on dendritic cells (DC) and endothelium. Along with CD226, the TIGIT:CD226/DNAM1 and PVR:PVR2 pairings appear to form a network that parallels the well-characterized B7-1:B7-2 and CD28:CTLA4 system. Binding of TIGIT by DC induces DC IL-10 release and inhibits IL-12 production. Ligation of TIGIT on T cells dampens TCR-mediated activation, while NK cell TIGIT ligation blocks NK cell cytotoxicity. Mature mouse TIGIT is a type I transmembrane protein 215 amino acids (aa) in length. It contains a 116 aa extracellular region (aa 26-141) with a V-type Ig-like domain (aa 27-125), and a 79 aa cytoplasmic domain with one ITIM motif. There is one isoform variant that is quite unusual and shows an addition of nine amino acids spread over three insertion sites (SwissProt #:P86176). Mouse and human TIGIT are highly divergent, and over aa 26-143, mouse TIGIT shares only 68% aa sequence identity with human TIGIT.

Long Name
T Cell Immunoreceptor with Ig and ITIM Domains
Entrez Gene IDs
201633 (Human); 100043314 (Mouse); 363784 (Rat); 102121588 (Cynomolgus Monkey)
Alternate Names
T cell immunoreceptor with Ig and ITIM domains; TIGIT; VSIG9; VSTM3; WUCAM

Product Datasheets

FAQs

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Reviews for Mouse TIGIT Antibody

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Mouse TIGIT Antibody
By Anonymous on 01/31/2019
Application: Flow Sample Tested: 4T1 mouse breast cancer cell line,4T1.2 mouse mammary carcinoma cells Species: Mouse

Antibodies primary and secondary incubated for 60 min at 4 C. The antibody was tested along rabbit isotype control. The analysis was done in FL1 channel using flow cytometry


Mouse TIGIT Antibody
By Anonymous on 12/16/2018
Application: Flow Sample Tested: CT-26 cell line Species: Mouse