Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2190A
View all TIGIT Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse TIGIT
Met1-Thr143
Accession # P86176

Specificity

Detects mouse TIGIT in direct ELISAs.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Mouse TIGIT Antibody

Detection of TIGIT on CD4+ Mouse Splenocytes antibody by Flow Cytometry.

Detection of TIGIT on CD4+ Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were surface stained with Rat anti-Mouse CD4 FITC-conjugated Monoclonal Antibody (Catalog # FAB554F) and either (A) Rabbit Anti-Mouse TIGIT Monoclonal Antibody (Catalog # MAB72671) or (B) Rabbit IgG control antibody (Catalog # MAB1050) followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). Then stained with Rabbit anti-Human/Mouse FoxP3 PE-conjugated Monoclonal Antibody (Catalog # IC8214P).

Applications for Mouse TIGIT Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Mouse CD4+ splenocytes

Reviewed Applications

Read 4 reviews rated 5 using MAB72671 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TIGIT

TIGIT (T cell Immunoreceptor with Ig and ITIM domains; also Vstm3 and Vsig9) is a 30-34 kDa (in human) member of the CD28 family, Ig superfamily of molecules. It is expressed by NK cells and multiple subsets of mature T cells, and binds to PVR/CD155 and PVR2/CD112 that appear on dendritic cells (DC) and endothelium. Along with CD226, the TIGIT:CD226/DNAM1 and PVR:PVR2 pairings appear to form a network that parallels the well-characterized B7-1:B7-2 and CD28:CTLA4 system. Binding of TIGIT by DC induces DC IL-10 release and inhibits IL-12 production. Ligation of TIGIT on T cells dampens TCR-mediated activation, while NK cell TIGIT ligation blocks NK cell cytotoxicity. Mature mouse TIGIT is a type I transmembrane protein 215 amino acids (aa) in length. It contains a 116 aa extracellular region (aa 26-141) with a V-type Ig-like domain (aa 27-125), and a 79 aa cytoplasmic domain with one ITIM motif. There is one isoform variant that is quite unusual and shows an addition of nine amino acids spread over three insertion sites (SwissProt #:P86176). Mouse and human TIGIT are highly divergent, and over aa 26-143, mouse TIGIT shares only 68% aa sequence identity with human TIGIT.

Long Name

T Cell Immunoreceptor with Ig and ITIM Domains

Alternate Names

VSIG9, VSTM3, WUCAM

Entrez Gene IDs

201633 (Human); 100043314 (Mouse); 363784 (Rat); 102121588 (Cynomolgus Monkey)

Gene Symbol

TIGIT

UniProt

P86176 (Mouse)

Additional TIGIT Products

Product Documents for Mouse TIGIT Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse TIGIT Antibody

For research use only

Customer Reviews for Mouse TIGIT Antibody (4)

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4 Customer Ratings
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Showing  1 - 4 of 4 reviews Showing All
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  • Mouse TIGIT Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: 4T1 mouse breast cancer cell line and 4T1.2 mouse mammary carcinoma cells
    Species: Mouse
    Verified Customer | Posted 01/31/2019
    Antibodies primary and secondary incubated for 60 min at 4 C. The antibody was tested along rabbit isotype control. The analysis was done in FL1 channel using flow cytometry
    Mouse TIGIT Antibody MAB72671
  • Mouse TIGIT Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: CT-26 cell line
    Species: Mouse
    Verified Customer | Posted 12/16/2018
    Mouse TIGIT Antibody MAB72671
  • Mouse TIGIT Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: 4T1 mouse breast cancer cell line
    Species: Mouse
    Verified Customer | Posted 11/16/2018
    Antibodies primary and secondary incubated for 60 min at 4 C. The antibody was tested along rabbit isotype control
    Mouse TIGIT Antibody MAB72671
  • Mouse TIGIT Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: MOuse cell line CT26
    Species: Mouse
    Verified Customer | Posted 10/24/2018
    Mouse TIGIT Antibody MAB72671

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