Mouse TMEFF1/Tomoregulin-1 Antibody

Catalog # Availability Size / Price Qty
TMEFF1/Tomoregulin‑1 in Mouse Spinal Cord.
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Product Details
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Mouse TMEFF1/Tomoregulin-1 Antibody Summary

Species Reactivity
Detects mouse TMEFF1/Tomoregulin‑1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 40% cross‑reactivity with recombinant human (rh) TMEFF1 is observed and less than 5% cross-reactivity with rhTMEFF2 is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant mouse TMEFF1/Tomoregulin‑1
Accession # Q6PFE7
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Mouse TMEFF1/Tomoregulin‑1
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry TMEFF1/Tomoregulin‑1 antibody in Mouse Spinal Cord by Immunohistochemistry (IHC-Fr). View Larger

TMEFF1/Tomoregulin‑1 in Mouse Spinal Cord. TMEFF1/Tomoregulin-1 was detected in perfusion fixed frozen sections of mouse spinal cord using 15 µg/mL Goat Anti-Mouse TMEFF1/Tomoregulin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1995) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TMEFF1/Tomoregulin-1

TMEFF1, also known as tomoregulin 1 is a type I transmembrane glycoprotein that contains two follistatin modules and an EGF domain in its extracellular domain, a transmembrane domain and a short cytoplasmic tail. The extracellular domain can be released as a soluble protein. TMEFF1 selectively regulates nodal but not activin signaling through direct binding to the nodal co-receptor, Cripto.

Long Name
Transmembrane Protein with EFG-like and two Follistatin-like Domains
Entrez Gene IDs
8577 (Human); 230157 (Mouse)
Alternate Names
C9orf2; H7365member 1; TMEFF1; Tomoregulin1; Tomoregulin-1; TR-1; Transmembrane protein with EGF-like and one follistatin-like domain; transmembrane protein with EGF-like and two follistatin-like domains 1

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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