Mouse TMEFF1/Tomoregulin-1 Antibody

AF1995 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF1995
AF1995-SP

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Mouse TMEFF1/Tomoregulin-1 Antibody in Immunohistochemistry

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Mouse TMEFF1/Tomoregulin-1 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse TMEFF1/Tomoregulin‑1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 40% cross‑reactivity with recombinant human (rh) TMEFF1 is observed and less than 5% cross-reactivity with rhTMEFF2 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse TMEFF1/Tomoregulin‑1
Ser37-Thr320
Accession # Q6PFE7

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse TMEFF1/Tomoregulin‑1

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry

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TMEFF1/Tomoregulin‑1 in Mouse Spinal Cord. TMEFF1/Tomoregulin-1 was detected in perfusion fixed frozen sections of mouse spinal cord using 15 µg/mL Goat Anti-Mouse TMEFF1/Tomoregulin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1995) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TMEFF1/Tomoregulin-1

TMEFF1, also known as tomoregulin 1 is a type I transmembrane glycoprotein that contains two follistatin modules and an EGF domain in its extracellular domain, a transmembrane domain and a short cytoplasmic tail. The extracellular domain can be released as a soluble protein. TMEFF1 selectively regulates nodal but not activin signaling through direct binding to the nodal co-receptor, Cripto.

Long Name

Transmembrane Protein with EFG-like and two Follistatin-like Domains

Entrez Gene IDs

8577 (Human); 230157 (Mouse)

Alternate Names

C9orf2; H7365member 1; TMEFF1; Tomoregulin1; Tomoregulin-1; TR-1; Transmembrane protein with EGF-like and one follistatin-like domain; transmembrane protein with EGF-like and two follistatin-like domains 1