Mouse TNF-alpha Antibody (TN3-19.12)

Name: Mouse TNF-alpha Antibody (TN3-19.12)
Brand: R&D Systems
SKU: MAB410
Price: 489 USD
Availability: InStock

R&D Systems | Catalog # MAB410

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Citations (12)

Product Documents

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Rat, Transgenic Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot, Neutralization, ELISA Capture

Label

Unconjugated

Antibody Source

Monoclonal Hamster IgG Clone # TN3-19.12

View all TNF-alpha Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse TNF-alpha

Specificity

Detects recombinant mouse (rm)TNF-alpha in direct ELISAs and Western blots. Shows 50% cross-reactivity with rrTNF-alpha and no cross-reactivity with 17 other tested proteins.

Clonality

Monoclonal

Host

Hamster

Isotype

IgG

Scientific Data Images for Mouse TNF-alpha Antibody (TN3-19.12)

Detection of Recombinant Mouse, and Rat TNF‑ alpha by Western Blot.

Western blot shows 25 ng of Recombinant Mouse TNF-a aa 80-235 (Catalog # 410-MT), Recombinant Human TNF-a (Catalog # 210-TA), Recombinant Rat TNF-a (Catalog # 510-RT) and Recombinant Mouse Lymphotoxin- a/TNF- beta. PVDF Membrane was probed with 1 µg/mL of Hamster Anti-Mouse TNF-a Monoclonal Antibody (Catalog # MAB410) followed by HRP-conjugated Anti-Hamster IgG Secondary Antibody. A specific band was detected for TNF-a at approximately 15 kDa (as indicated). This experiment was conducted under reducing and non reducing conditions using Immunoblot Buffer Group 3.

Detection of Mouse TNF-alpha by Immunohistochemistry

Inflammatory response induced by HI injury and maternal SE. Representative images of immunofluorescence staining of inflammatory cytokines IL-1 beta, IL-6, and TNF alpha in the cerebral cortex (A) and the hippocampus (E). The immunofluorescence intensity of IL-1 beta (B,F), IL-6 (C,G), and TNF alpha (D,H) in the cerebral cortex and hippocampus. Results are presented as mean ± SEM. *P < 0.05, **P < 0.01, n = 4, analyzed by two-way ANOVA followed by post hoc Turkey tests. SH, from sham exposed dams with sham surgery; HI, hypoxic-ischemic injury; SE, from smoke exposed dams with sham surgery; HI + SE, from smoke exposed dams with hypoxic-ischemic injury. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35250486), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse TNF-alpha Antibody (TN3-19.12)

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse TNF‑ alpha (Catalog # 410-MT)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: TNF-alpha

Tumor necrosis factor alpha (TNF-alpha, TNF- alpha, TNFA ), also known as Cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism. TNF-alpha is produced by several lymphoid cells as well as by astrocytes, endothelial cells, and smooth muscle cells. Mouse TNF-alpha consists of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 179 aa extracellular domain (ECD). Within the ECD, mouse TNF-alpha shares 94% aa sequence identity with rat and 70%-77% with bovine, canine, cotton rat, equine, feline, human, porcine, and rhesus TNF-alpha. TNF-alpha is produced by a wide variety of immune, epithelial, endothelial, and tumor cells. TNF-alpha is assembled intracellularly to form a noncovalently linked homotrimer which is expressed on the cell surface. Cell surface TNF-alpha can induce the lysis of neighboring tumor cells and virus infected cells, and it can generate its own downstream cell signaling following ligation by soluble TNFR I. Shedding of membrane bound TNF-alpha by TACE/ADAM17 releases the bioactive cytokine, a 55 kDa molecular weight soluble trimer of the TNF-alpha extracellular domain. TNF-alpha binds the ubiquitous 55-60 kDa TNF RI and the hematopoietic cell-restricted 80 kDa TNF RII, both of which are also expressed as homotrimers present on virtually all cell types. Both type I and type II receptors bind TNF-alpha with comparable affinity, although only TNF RI contains a cytoplasmic death domain which triggers the activation of apoptosis. Soluble forms of both types of receptors are released and can neutralize the biological activity of TNF-alpha.

Long Name

Tumor Necrosis Factor alpha

Alternate Names

Cachetin, DIF, TNF, TNF-A, TNFA, TNFalpha, TNFG1F, TNFSF1A, TNFSF2

Entrez Gene IDs

7124 (Human); 21926 (Mouse); 24835 (Rat); 397086 (Porcine); 280943 (Bovine); 403922 (Canine); 102139631 (Cynomolgus Monkey); 100033834 (Equine); 493755 (Feline); 100009088 (Rabbit)