Mouse TNF-alpha Fluorescein-conjugated Antibody Summary
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of TNF-alpha in EL‑4 Mouse Cell Line by Flow Cytometry. EL‑4 mouse lymphoblast cell line treated with anti-CD3/anti-CD28, PMA, and Calcium Ionomycin were stained with Rat Anti-Mouse TNF-alpha Fluorescein‑conjugated Monoclonal Antibody (Catalog # IC410F, filled histogram) or isotype control antibody (Catalog # IC005F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
TNF-alpha is a trimeric glycoprotein active in both membrane bound and secreted forms. TNF-alpha is produced by several lymphoid cells as well as by astrocytes, endothelial cells, and smooth muscle cells. TNF-alpha binds to TNF RI and TNF RII present on virtually all cell types, through which it triggers the activation of multiple signal transduction pathways and modulates the expression of a wide variety of genes.
Citation for Mouse TNF-alpha Fluorescein-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Tumor necrosis factor-alpha does not modulate ischemia/reperfusion injury in naÃƒÂ¯ve myocardium but is essential for the development of late preconditioning.
Authors: Dawn B, Guo Y, Rezazadeh A, Wang OL, Stein AB, Hunt G, Varma J, Xuan YT, Wu WJ, Tan W, Zhu X, Bolli R
J. Mol. Cell. Cardiol., 2004;37(1):51-61.
Sample Types: Whole Tissue
Applications: IHC Paraffin-embedded
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