Mouse TNF-alpha Antibody

(10 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse TNF-alpha.
  • Source
    Monoclonal Rat IgG1 Clone # MP6-XT22
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    mouse TNF-alpha
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Neutralization
    Measured by its ability to neutralize TNF‑ alpha -induced cytotoxicity in the L‑929 mouse fibroblast cell line. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The Neutralization Dose (ND50) is typically 0.15-0.75 µg/mL in the presence of 0.25 ng/mL Recombinant Mouse TNF‑ alpha and 1 µg/mL actinomycin D.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cytotoxicity Induced by TNF‑ alpha and Neutralization by Mouse TNF‑ alpha Antibody. Recombinant Mouse TNF‑ alpha (Catalog # 410-MT) induces cytotoxicity in the the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Mouse TNF‑ alpha (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse TNF‑ alpha Monoclonal Antibody (Catalog # MAB4101). The ND50 is typically 0.15-0.75 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TNF-alpha

TNF-alpha is a trimeric glycoprotein active in both membrane bound and secreted forms. TNF-alpha is produced by several lymphoid cells as well as by astrocytes, endothelial cells, and smooth muscle cells. TNF-alpha binds to TNF RI and TNF RII present on virtually all cell types, through which it triggers the activation of multiple signal transduction pathways and modulates the expression of a wide variety of genes.

  • Long Name:
    Tumor Necrosis Factor alpha
  • Entrez Gene IDs:
    7124 (Human); 21926 (Mouse); 24835 (Rat); 397086 (Porcine); 280943 (Bovine); 403922 (Canine); 100033834 (Equine); 493755 (Feline); 100009088 (Rabbit)
  • Alternate Names:
    APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; tnfa; tnf-a; TNFalpha; TNF-alpha; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Induction of necroptotic cell death by viral activation of the RIG-I or STING pathway
    Authors: SN Schock, NV Chandra, Y Sun, T Irie, Y Kitagawa, B Gotoh, L Coscoy, A Winoto
    Cell Death Differ, 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  2. Presentation of Cryptic Peptides by MHC Class I Is Enhanced by Inflammatory Stimuli
    J Immunol, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  3. Myostatin is a direct regulator of osteoclast differentiation and its inhibition reduces inflammatory joint destruction in mice.
    Authors: Dankbar B, Fennen M, Brunert D, Hayer S, Frank S, Wehmeyer C, Beckmann D, Paruzel P, Bertrand J, Redlich K, Koers-Wunrau C, Stratis A, Korb-Pap A, Pap T
    Nat Med, 2015;21(9):1085-90.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  4. MyD88 signalling in myeloid cells is sufficient to prevent chronic mycobacterial infection.
    Authors: Berod L, Stuve P, Swallow M, Arnold-Schrauf C, Kruse F, Gentilini M, Freitag J, Holzmann B, Sparwasser T
    Eur J Immunol, 2014;44(5):1399-409.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  5. A paracrine network regulates the cross-talk between human lung stem cells and the stroma.
    Authors: Ruiz E, Oeztuerk-Winder F, Ventura J
    Nat Commun, 2014;5(0):3175.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  6. Acute lung injury and acute kidney injury are established by four hours in experimental sepsis and are improved with pre, but not post, sepsis administration of TNF-alpha antibodies.
    Authors: Bhargava R, Altmann C, Andres-Hernando A, Webb R, Okamura K, Yang Y, Falk S, Schmidt E, Faubel S
    PLoS ONE, 2013;8(11):e79037.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  7. Critical involvement of extracellular ATP acting on P2RX7 purinergic receptors in photoreceptor cell death.
    Authors: Notomi S, Hisatomi T, Kanemaru T, Takeda A, Ikeda Y, Enaida H, Kroemer G, Ishibashi T
    Am. J. Pathol., 2011;179(6):2798-809.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  8. Toll-like receptor 3 ligand dampens liver inflammation by stimulating Valpha 14 invariant natural killer T cells to negatively regulate gammadeltaT cells.
    Authors: Gardner TR, Chen Q, Jin Y, Ajuebor MN
    Am. J. Pathol., 2010;176(4):1779-89.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  9. Relative importance of T-cell subsets in monocytotropic ehrlichiosis: a novel effector mechanism involved in Ehrlichia-induced immunopathology in murine ehrlichiosis.
    Authors: Ismail N, Crossley EC, Stevenson HL, Walker DH
    Infect. Immun., 2007;75(9):4608-20.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  10. CCL16/LEC powerfully triggers effector and antigen-presenting functions of macrophages and enhances T cell cytotoxicity.
    Authors: Cappello P, Caorsi C, Bosticardo M
    J. Leukoc. Biol., 2004;75(1):135-42.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
Isotype Controls
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Rat IgG1 Isotype Control

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Normal Rat IgG Control

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Normal Rat IgG Control (Azide Free)

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Rat IgG1 Isotype Control

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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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