Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse, Hamster

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Neutralization, Immunocytochemistry, Blocking, IHC fixed frozen, IHC Paraffin-embedded

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all VLDLR Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse VLDL R
Thr25-Ala798
Accession # AAA59384

Specificity

Detects mouse VLDL R in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse VLDLR Antibody

VLDL R antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr).

VLDL R in Mouse Embryo.

VLDL R was detected in immersion fixed frozen sections of mouse embryo using Mouse VLDL R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2258) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

VLDL R antibody in Mouse Kidney by Immunohistochemistry (IHC-Fr).

VLDL R in Mouse Kidney.

VLDL R was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse VLDL R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2258) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes of convoluted tubules. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of VLDLR by Western Blot

Detection of VLDLR by Western Blot

The increase in CD36 levels caused by lipids in Sirt3-deficient hepatocytes is mediated by Nrf2. VLDLR mRNA abundance (a) and protein levels of VLDLR and NQO1, an Nrf-2-target gene, (b) were assessed in Huh-7 cells incubated with fatty acid free-BSA or BSA-palmitate (0.3 mM) and exposed to either vehicle or the Sirt3 inhibitor AAPBO (100 μM) for 16 h. a, p < 0.05 vs. CT. b, p < 0.05 vs. CT cells incubated with palmitate. c, p < 0.05 vs. CT cells treated with AAPBO. c, fatty acid uptake in Huh-7 cells incubated with fatty acid free-BSA or BSA-palmitate (0.3 mM) and exposed to either vehicle or the Sirt3 inhibitor AAPBO (100 μM) for 16 h was measured by the uptake of BODIPY-C16. a, p < 0.05 vs. CT. b, p < 0.05 vs. CT cells incubated with palmitate. c, p < 0.05 vs. CT cells treated with AAPBO. mRNA abundance (d) and protein levels of VLDLR (e) in Huh-7 cells transfected with control (CT) or SIRT3 siRNA and incubated in the presence or absence 0.3 mM palmitate (Pal) for 24 h. Protein levels of CD36 (f), NQO1 (g) and PPAR gamma (h) in Huh-7 cells transfected with control (CT) or SIRT3 siRNA and incubated in the presence or absence 0.3 mM palmitate (Pal) or the Nrf2 inhibitor ML385 (10 μM) for 24 h. a, p < 0.05 vs. CT siRNA cells. b, p < 0.05 vs. CT siRNA cells incubated with palmitate. c, p < 0.05 vs. SIRT3 siRNA cells. d, p < 0.05 vs. CT siRNA cells incubated with palmitate and ML385 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32912335), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse VLDLR by Western Blot

Detection of Mouse VLDLR by Western Blot

SIRT1 inhibition increases VLDLR levels and VLDL uptake in human Huh-7 cells. (A) mRNA and (B) immunoblot analysis of VLDLR in human Huh-7 cells in the absence (control, CT) or presence of 10 µM EX-527 for 24 h. Immunoblot analysis of (C) VLDLR and (D) VLDL uptake in human Huh-7 cells in the absence (control, CT) or presence of 10 µM EX-527, or in the presence of both 10 µM EX-527 and 20 µM PX-478 for 24 h. (E) Immunoblot analysis of SIRT1 and VLDLR in Huh-7 cells transfected with control siRNA or SIRT1 siRNA in the absence or presence of 20 µM PX-478. Data are presented as the mean ± SEM. Significant differences were established by Student’s t-test or one-way ANOVA with Tukey’s post-hoc test. *p < 0.05 and **p < 0.01 vs. CT. #p < 0.05, ##p < 0.01, and ###p < 0.001 vs. EX-527 or SIRT1 siRNA. n = 3 or 4 per group Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38807218), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse VLDLR Antibody

Application
Recommended Usage

Immunohistochemistry

3-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (E13.5-15.5) and perfusion fixed frozen sections of mouse kidney

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse VLDL R (Catalog # 2258-VL)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VLDLR

VLDL R is a 105 kDa type I integral membrane protein that belongs to the LDL receptor family. It plays a significant role in lipid metabolism and in nervous system development and function (1, 2). Mouse VLDL R has a 770 amino acid (aa) extracellular domain (ECD) and a 54 aa cytoplasmic region. The ECD contains eight LDLR class A repeats, three EGF-like repeats, six LDLR class B repeats, and a juxtamembrane region that is rich in O-linked glycosylation (3, 4). The cytoplasmic domain contains one NPXY internalization motif. VLDL R is predominantly expressed in striated muscle, adipose tissue, brain, and endothelial cells lining capillaries and small arterioles (3-6). VLDL R participates in the tissue uptake of fatty acids from plasma by mediating the internalization of ApoE-containing lipoparticles (i.e. VLDL, beta ‑VLDL, and chylomicron remnants) (5, 7). VLDL R binds and internalizes lipoprotein lipase (LPL) and mediates its transport from the basolateral to the lumenal face of endothelial cells (6, 8). VLDL R knockout mice are characterized by reduced LPL activity, reduced serum triglyceride clearance, and a resistance to developing obesity (7, 9, 10). VLDL R influences breast cancer cell motility by mediating the uptake of uPAR-PAI1 complexes (6, 11). Lipoprotein accumulation via macrophage VLDL R is instrumental in promoting the formation of atherosclerotic plaques (12). In the nervous system, VLDL R and ApoE R2 interactions with Reelin are critical for neuronal migration and positioning in the developing brain (13). VLDL R also functions in adult hippocampal synapse maturation, synaptic plasticity, and memory formation (14, 15). The ECD of mouse VLDL R shares 95% aa sequence identity with human and rat VLDL R. Within shared regions, mouse VLDL R shares 55% and 53% aa sequence identity with ApoE R2 and LDL R, respectively.

References

  1. Qiu, S. et al. (2006) Neurobiol. Learn. Mem. 85:16.
  2. May, P. et al. (2005) Cell. Mol. Life Sci. 62:2325.
  3. Gafvels, M.E. et al. (1994) Endocrinology 135:387.
  4. Oka, K. et al. (1994) Eur. J. Biochem. 224:975.
  5. Wyne, K.L. et al. (1996) Arterioscler. Thromb. Vasc. Biol. 16:407.
  6. Argraves, K.M. et al. (1995) J. Biol. Chem. 270:26550.
  7. Goudriaan, J.R. et al. (2001) Arterioscler. Thromb. Vasc. Biol. 21:1488.
  8. Obunike, J.C. et al. (2001) J. Biol. Chem. 276:8934.
  9. Yagyu, H. et al. (2002) J. Biol. Chem. 277:10037.
  10. Goudriaan, J.R. et al. (2004) J. Lipid Res. 45:1475.
  11. Webb, D.J. et al. (1999) J. Biol. Chem. 274:7412.
  12. van Eck, M. et al. (2005) Atherosclerosis 183:230.
  13. Jossin, Y. et al. (2004) J. Neurosci. 24:514.
  14. Niu, S. et al. (2004) Neuron 41:71.
  15. Weeber, E.J. et al. (2002) J. Biol. Chem. 277:39944.

Long Name

Very Low Density Lipoprotein Receptor

Alternate Names

CHRMQ1, VLDL R, VLDLRCH

Entrez Gene IDs

7436 (Human); 22359 (Mouse)

Gene Symbol

VLDLR

UniProt

AAA59384

Additional VLDLR Products

Product Documents for Mouse VLDLR Antibody

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Product Specific Notices for Mouse VLDLR Antibody

For research use only

Related Research Areas

Obesity

Citations for Mouse VLDLR Antibody

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Protocols

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