Oxidized PTP Active Site Antibody

(6 citations)   
  • Specificity
    Detects Oxidized PTP Active Site. Will react with any oxidized phosphatase containing the consensus sequence. Detects oxidized but not unoxidized DEP-1, PTP1B, TC-PTP, and SHP-2. Other phosphatases have not been tested.
  • Source
    Monoclonal Mouse IgG1 Clone # 335636
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    KLH-coupled perfomic acid oxidized synthetic peptide
    VHCSAG
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Oxidized PTP by Western Blot. Western blot shows oxidized PTP immunoprecipitated from lysates of HepG2 human hepatocellular carcinoma cell line using Rat Anti-SHP-2 antibody. PVDF membrane was probed with 1 µg/mL Mouse Anti-Oxidized PTP Active Site Monoclonal Antibody (Catalog # MAB2844) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for Oxidized SHP-2 was detected at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Oxidized PTP Active Site

Oxidation of the essential cysteine in the active site of protein tyrosine phosphatases (PTPs) by exogenous or intracellular oxidants such as hydrogen peroxide is believed to play an important role in regulating the activity of these enzymes. The initial oxidation to a sulfenic acid (-SOH) is reversible, but the oxidation often progresses to an irreversible sulfonic acid (-SO3H) that can be detected by modification-specific antibodies. The active sites of PTPs contain the consensus amino acid sequence (V/I)HCSXG, a sequence essential for catalytic activity.

  • Long Name:
    Oxidized Protein Tyrosine Phosphatase Active Site
  • Alternate Names:
    Oxidized PTP Active Site
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Species
Applications
Sample Type
  1. THEMIS enhances TCR signaling and enables positive selection by selective inhibition of the phosphatase SHP-1
    Authors: S Choi, C Warzecha, E Zvezdova, J Lee, J Argenty, R Lesourne, L Aravind, PE Love
    Nat. Immunol, 2017;0(0):.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. Differential oxidation of protein-tyrosine phosphatases during zebrafish caudal fin regeneration
    Authors: W Wu, AJ Hale, S Lemeer, J den Hertog
    Sci Rep, 2017;7(1):8460.
    Species: Zebrafish
    Sample Type: Tissue Homogenates
    Application: IP
  3. Dual oxidase 1 induced by Th2 cytokines promotes STAT6 phosphorylation via oxidative inactivation of protein tyrosine phosphatase 1B in human epidermal keratinocytes.
    Authors: Hirakawa S, Saito R, Ohara H
    J. Immunol., 2011;186(8):4762-70.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  4. Redox regulation of interleukin-4 signaling.
    Authors: Sharma P, Chakraborty R, Wang L, Min B, Tremblay ML, Kawahara T, Lambeth JD, Haque SJ
    Immunity, 2008;29(4):551-64.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  5. Cysteine S-nitrosylation protects protein-tyrosine phosphatase 1B against oxidation-induced permanent inactivation.
    Authors: Chen YY, Chu HM, Pan KT, Teng CH, Wang DL, Wang AH, Khoo KH, Meng TC
    J. Biol. Chem., 2008;283(50):35265-72.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
Isotype Controls
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Mouse IgG1 Isotype Control

Ctrl MAB002 138  
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Mouse IgG1 Isotype Control

Ctrl MAB002R 1
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Secondary Antibodies
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Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse IgG Antibody

WB AF007 4
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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