Detects Oxidized PTP Active Site. Will react with any oxidized phosphatase containing the consensus sequence. Detects oxidized but not unoxidized DEP-1, PTP1B, TC-PTP, and SHP-2. Other phosphatases have not been tested.
Monoclonal Mouse IgG1 Clone # 335636
Protein A or G purified from hybridoma culture supernatant
Detection of Oxidized PTP by Western Blot. Western blot shows oxidized PTP immunoprecipitated from lysates of HepG2 human hepatocellular carcinoma cell line using Rat Anti-SHP-2 antibody. PVDF membrane was probed with 1 µg/mL Mouse Anti-Oxidized PTP Active Site Monoclonal Antibody (Catalog # MAB2844) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for Oxidized SHP-2 was detected at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Oxidized PTP Active Site
Oxidation of the essential cysteine in the active site of protein tyrosine phosphatases (PTPs) by exogenous or intracellular oxidants such as hydrogen peroxide is believed to play an important role in regulating the activity of these enzymes. The initial oxidation to a sulfenic acid (-SOH) is reversible, but the oxidation often progresses to an irreversible sulfonic acid (-SO3H) that can be detected by modification-specific antibodies. The active sites of PTPs contain the consensus amino acid sequence (V/I)HCSXG, a sequence essential for catalytic activity.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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